BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0263.0021.p (268 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C3FB73.1 C3FB73_BACTU Peptidase S8 and S53 subtilisin kexin sedo... 187 1e-45 C3HT26.1 C3HT26_BACTU Peptidase S8 and S53 subtilisin kexin sedo... 182 3e-44 B0J6R9.1 B0J6R9_RHILT Peptidase S8 and S53 subtilisin kexin sedo... 134 1e-29 A7HBB8.1 A7HBB8_ANADF Peptidase S8 and S53 subtilisin kexin sedo... 132 4e-29 A0NNN6.1 A0NNN6_9RHOB Peptidase S8 and S53, subtilisin, kexin, s... 85 6e-15 >C3FB73.1 C3FB73_BACTU Peptidase S8 and S53 subtilisin kexin sedolisin Length = 609 Score = 187 bits (474), Expect = 1e-45, Method: Compositional matrix adjust. Identities = 114/274 (41%), Positives = 156/274 (56%), Gaps = 30/274 (10%) Query: 14 PSHLDPRLQKTIARSQAGXXXXXXXXXXXXXLVSVIAKVKNVQTFLDLPGVYQGKRITLA 73 PS +DPRLQ+ I +SQ VSVIAKVK++ + ++PGV+Q I+ A Sbjct: 11 PSSIDPRLQRIIEQSQREVEIREVSTDAAKD-VSVIAKVKDINEWSNIPGVHQTNPISSA 69 Query: 74 PDKSGHIVTAKVSVSRLEQIQQSSVVMTLSSAQPIKLMLKDTLREIG----LSAPGPLEM 129 PDKS IVTAKVSV+ LE I+QS V++L +AQP++ LK T+ E+ L PG Sbjct: 70 PDKSSQIVTAKVSVNELEAIRQSPTVISLKAAQPVQTALKKTIEEVSARNDLLPPGVARD 129 Query: 130 XXXXXXXXXXXDHGCDFAHQNFRNSDGTTRLLSLMTQSVNLINGQTKEI--IYTPDKINT 187 D GCDF H+NFRN+DG+TR+L++ Q+ IN + ++ ++IN Sbjct: 130 HGGQGVVVGIIDSGCDFVHKNFRNTDGSTRILTIWDQAAERINDSSIPYGRVFNREEINN 189 Query: 188 ALQNINPYQQLGY------------DPGYSSHGTHVMDIAAGNGLAKMSPGVAPKADIIF 235 AL + PY+ LGY PG+ HGTHVMDIAAGNG +PGVAP AD++F Sbjct: 190 ALSSSRPYKTLGYPASGSFPANPPKPPGH-KHGTHVMDIAAGNGQGTGTPGVAPSADLVF 248 Query: 236 VD-------PDYRDIQNGELGNFADSRNLLNSVK 262 V+ + RD+ LG DS LL++VK Sbjct: 249 VELAANDIPWEGRDVVGSTLG---DSAQLLDAVK 279 >C3HT26.1 C3HT26_BACTU Peptidase S8 and S53 subtilisin kexin sedolisin Length = 613 Score = 182 bits (462), Expect = 3e-44, Method: Compositional matrix adjust. Identities = 114/277 (41%), Positives = 154/277 (55%), Gaps = 32/277 (11%) Query: 14 PSHLDPRLQKTIARSQ---AGXXXXXXXXXXXXXLVSVIAKVKNVQTFLDLPGVYQGKRI 70 PS +DPRLQ+ I +SQ A VSVIAKVK++ + +PGV Q I Sbjct: 11 PSSIDPRLQRIIEQSQREVAREMEIRTSSTDAVKNVSVIAKVKDIDEWNGIPGVLQTNPI 70 Query: 71 TLAPDKSGHIVTAKVSVSRLEQIQQSSVVMTLSSAQPIKLMLKDTLREIG----LSAPGP 126 + APDKS IVTAKVSV+ LE I+QS V++L +AQP++ LK T+ E+ L PG Sbjct: 71 SSAPDKSSQIVTAKVSVNELEAIRQSPTVISLKAAQPVQTALKKTIEEVSARNDLLPPGV 130 Query: 127 LEMXXXXXXXXXXXDHGCDFAHQNFRNSDGTTRLLSLMTQSVNLINGQTKEI--IYTPDK 184 D GCDF H+NFRN+DG+TR+L++ Q+ IN + ++ ++ Sbjct: 131 ARDHGGQGVVVGIIDSGCDFVHKNFRNTDGSTRILTIWDQAAERINDSSIPYGRVFNREE 190 Query: 185 INTALQNINPYQQLGY------------DPGYSSHGTHVMDIAAGNGLAKMSPGVAPKAD 232 IN AL + PY+ L Y PG+ HGTHVMDIAAGNG +PGVAP AD Sbjct: 191 INNALSSSRPYKTLSYPASGSFPANPPKPPGH-KHGTHVMDIAAGNGQGTGTPGVAPSAD 249 Query: 233 IIFVD-------PDYRDIQNGELGNFADSRNLLNSVK 262 ++FV+ + RD+ LG DS LL++VK Sbjct: 250 LVFVELAANDIPWEGRDVVGSTLG---DSAQLLDAVK 283 >B0J6R9.1 B0J6R9_RHILT Peptidase S8 and S53 subtilisin kexin sedolisin Length = 654 Score = 134 bits (337), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 96/273 (35%), Positives = 136/273 (49%), Gaps = 24/273 (8%) Query: 12 NPPSHLDPRLQKTIARSQAGXXXXXXXXXXXXXLVSVIAKVKNVQTFLDL----PGVYQG 67 N + +DPRLQ I +G V+VIA+V +V +++L PG G Sbjct: 62 NVTASMDPRLQLAIINRLSGKPGLSLTSTAGNE-VAVIARVDSVDDWINLEDVIPGAMLG 120 Query: 68 KRITLAP-DKSGHIVTAKVSVSRLEQIQQSSVVMTLSSAQPIKLMLKDTLREI-----GL 121 K ++ D IVT ++ + R+E ++ V++L ++QP++ L TL + L Sbjct: 121 KANPISDNDPECWIVTGRLPIERIEHVRTQPSVISLKASQPVQPTLAATLPAMQVTAADL 180 Query: 122 SAPGPLEMXXXXXXXXXXXDHGCDFAHQNFRNSDGTTRLLSLMTQSVNLINGQTKEI--I 179 +A G ++ D GCDF H NFR +DG+TRLL++ Q G + Sbjct: 181 AAAG-VDPAGGAGVVIGIVDFGCDFMHANFRRADGSTRLLAIWNQGAVAQVGSPFGYGQL 239 Query: 180 YTPDKINTALQNINPYQQLGYDP------GYSSHGTHVMDIAAGNGLAKMSPGVAPKADI 233 Y IN AL NPY LGY P +HGTHVMDIAAGNGL PG+AP AD+ Sbjct: 240 YDSASINAALGKANPYDALGYGPRPDNPSQTGTHGTHVMDIAAGNGLGSNQPGMAPAADL 299 Query: 234 IFVDPDYRDI--QNGELGN--FADSRNLLNSVK 262 +FV+ DI Q + N F DS LL +V+ Sbjct: 300 VFVEASTTDIAWQGPSVVNQSFGDSVQLLEAVR 332 >A7HBB8.1 A7HBB8_ANADF Peptidase S8 and S53 subtilisin kexin sedolisin Length = 618 Score = 132 bits (331), Expect = 4e-29, Method: Compositional matrix adjust. Identities = 90/255 (35%), Positives = 130/255 (50%), Gaps = 11/255 (4%) Query: 17 LDPRLQKTIARSQAGXXXXXXXXXXXXXLVSVIAKVKNVQTFLDLPGVYQGKRITLAPDK 76 LDPRLQ I R + G + VIA+V + + L + +G + A D Sbjct: 12 LDPRLQALIERKRRGFIPRATASTDRGE-IPVIARVADPAAWRALADIREGTELGPARD- 69 Query: 77 SGHIVTAKVSVSRLEQIQQSSVVMTLSSAQPIKLMLKDTLREIGLSA---PGPLEMXXXX 133 G +VT +V V R+E ++Q V++L +A ++ L T R +G P Sbjct: 70 GGVLVTGRVPVDRIESLRQEPTVLSLKAATLLRPTLDATTRSMGARKTLLPAGARGAQGQ 129 Query: 134 XXXXXXXDHGCDFAHQNFRNSDGTTRLLSLMTQ-SVNLINGQTK-EIIYTPDKINTALQN 191 D GCDF H+NFRN DG+TRLL+L Q G+ ++ I+ AL+ Sbjct: 130 GVVIGVIDFGCDFVHRNFRNPDGSTRLLALWDQDGTPRAKGRAGYGRVHERADIDKALRA 189 Query: 192 INPYQQLGYDPGYSSHGTHVMDIAAGNGLAKMSPGVAPKADIIFVDPDYRDI-QNGE--L 248 +PY L Y+P SHGTHV DIAAGNG +PGVAP AD++FV+ D+ +G + Sbjct: 190 KDPYAALRYEPEPGSHGTHVTDIAAGNGRGTGTPGVAPNADLVFVELSAGDLPWDGPDVV 249 Query: 249 G-NFADSRNLLNSVK 262 G F DS +L+ ++K Sbjct: 250 GRTFGDSVHLVEALK 264 >A0NNN6.1 A0NNN6_9RHOB Peptidase S8 and S53, subtilisin, kexin, sedolisin Length = 641 Score = 85.1 bits (209), Expect = 6e-15, Method: Compositional matrix adjust. Identities = 63/206 (30%), Positives = 97/206 (47%), Gaps = 29/206 (14%) Query: 77 SGHIVTAKVSVSRLEQIQQSSVVMTLSSAQPIKLMLK--------DTLREIGLSAPGPLE 128 +G I + + ++RL ++ +V T+ + + ML+ DT+ + PG + Sbjct: 80 AGKIYSVSLPMNRLVELNGHPLVETVEAGRRWFPMLESSLAETKADTVHQGSSGVPGRM- 138 Query: 129 MXXXXXXXXXXXDHGCDFAHQNFRNSDGTTRLLSLMTQSVNLINGQTKE------IIYTP 182 D+G DF +FRN DGTTRL L Q ++ G+ + Y Sbjct: 139 ---GAGVLVGIIDYGMDFTLDDFRNPDGTTRLAFLWDQRLDARQGERAPDDFAYGVEYGA 195 Query: 183 DKINTALQNINPYQQLGYDPGYSSHGTHVMDIAAGNGLAK-------MSPGVAPKADIIF 235 D+IN AL +P+ + + SHGTHV IAAGNG + G AP+A +IF Sbjct: 196 DQINQALNATDPFSVVRHRAEAGSHGTHVAGIAAGNGRSHDDNFAEGTHLGAAPEATLIF 255 Query: 236 VDPDYRDIQNGELGNFADSRNLLNSV 261 V PD RD N F DS +++++V Sbjct: 256 VQPDTRDGSN----TFTDSSHVVDAV 277 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.133 0.373 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,352,242,266 Number of extensions: 46741643 Number of successful extensions: 112869 Number of sequences better than 1.0e-05: 20 Number of HSP's gapped: 112916 Number of HSP's successfully gapped: 20 Length of query: 268 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 135 Effective length of database: 1,807,701,208 Effective search space: 244039663080 Effective search space used: 244039663080 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 130 (54.7 bits)