BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0263.0011.p (398 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q8TNL4.1 Q8TNL4_METAC Putative uncharacterized protein 341 6e-92 Q1ZCQ6.1 Q1ZCQ6_9GAMM Twin-arginine translocation pathway signal... 201 7e-50 Q28QE0.1 Q28QE0_JANSC Twin-arginine translocation pathway signal 168 9e-40 B5J893.1 B5J893_9RHOB Putative uncharacterized protein 152 5e-35 A9CWU2.1 A9CWU2_9RHIZ Twin-arginine translocation pathway signal... 147 1e-33 >Q8TNL4.1 Q8TNL4_METAC Putative uncharacterized protein Length = 393 Score = 341 bits (875), Expect = 6e-92, Method: Compositional matrix adjust. Identities = 171/374 (45%), Positives = 240/374 (64%), Gaps = 7/374 (1%) Query: 27 ALFTASGVFKKAKYLDPWKRDYVDQFKDPRVKLAAYGLLAPNGHNLQPWKIRLDKQNPLK 86 +L GVF KYL+PW Y QF+DPRV++ A+G+LAPN HNLQ WK+ L+ ++ Sbjct: 21 SLAMVDGVFLPKKYLEPWSGTYHTQFEDPRVQVLAHGVLAPNSHNLQSWKVVLEGED--S 78 Query: 87 FSLYTDSSRLALESDPVARQTMVSQGTFLEYLRVGGEQLGYRAAIRLFPEGEYDEQKLSG 146 F LY D SRL+ ++DP RQ +SQGTFLEY+RV LGY A + LFP GEY + + Sbjct: 79 FFLYVDPSRLSPKADPPGRQVTISQGTFLEYVRVAAGNLGYEAEMELFPGGEYGPEGTAI 138 Query: 147 SMDQKPVAEITLSK----AQPAANPLFDYMFLPDTNRMAYKPDPLTAEQRQRLEQTNADG 202 ++ KPVA + L + +P ++PL+ MF+PDT R+ Y+ L+AE + LE + + Sbjct: 139 NLGSKPVARVILKENSRETEPESSPLYKSMFVPDTYRVPYRETQLSAEDIETLEALSTEN 198 Query: 203 ALKLIFLDAPEDIKKLGEYSLEGTDIETGLKRMNDESADIFRSNESQKNKYRSGFSFEGQ 262 LIF D PE ++ LG L ++E G + E ++FR NE +KN+YR GFS +GQ Sbjct: 199 VTILIFQD-PESLESLGNIVLRAAEVEAGNDGIQQEGFELFRPNEWKKNEYRYGFSLDGQ 257 Query: 263 GTTGVKKHLLQGMITIVPSFNNEEASTKLAVDAARTAAEHSPAYALILSKDNSRTSQVKA 322 G + HL+QG+I++ P N+ E S K + +A E++PAYALI++K+NSR +QV+A Sbjct: 258 GLPALNVHLMQGLISLFPFLNSPEVSGKSFLSQTESAVENTPAYALIITKNNSRAAQVEA 317 Query: 323 GMLYSRLLLAGHEQGLVMQPLSQVLEEYPEMKAPYTRIHQEYAPGGETIQFLIRLGQPAQ 382 G+LYSR LL + G MQP+SQ LEEYPEM YT IH+ YA ETIQ LIR+G P + Sbjct: 318 GILYSRFLLTATDMGYAMQPMSQALEEYPEMAEIYTEIHEAYAGEDETIQMLIRVGVPEK 377 Query: 383 DTPLSMRREVTDLI 396 + +MR +V D+I Sbjct: 378 EVFPTMRLDVIDII 391 >Q1ZCQ6.1 Q1ZCQ6_9GAMM Twin-arginine translocation pathway signal sequence domain protein Length = 386 Score = 201 bits (512), Expect = 7e-50, Method: Compositional matrix adjust. Identities = 124/350 (35%), Positives = 182/350 (52%), Gaps = 10/350 (2%) Query: 50 DQFKDPRVKLAAYGLLAPNGHNLQPWKIRLDKQNPLKFSLYTDSSRLALESDPVARQTMV 109 D D +K+ AY +L PN HN QPW I+++ +P F LY D +RL E+DP RQ + Sbjct: 42 DSKSDIYLKVLAYAILCPNPHNKQPWLIKVN--HPKSFDLYIDQTRLLPETDPFYRQIHI 99 Query: 110 SQGTFLEYLRVGGEQLGYRAAIRLFPEGEYDEQKLSGSMDQKPVAEITLSK-AQPAANPL 168 QG FLE L + LGY A I FP+GEY L KP+A I L+K + +PL Sbjct: 100 GQGCFLEMLSIAATGLGYHAEIEYFPQGEYKNTVLKS----KPIARIVLTKHIKIDIDPL 155 Query: 169 FDYMFLPDTNRMAYKPDPLTAEQRQRLEQTNADGALKLIFLDAPEDIKKLGEYSLEGTDI 228 F+Y+ +N+ Y LT +Q+ +L+ L D+ D + L E I Sbjct: 156 FNYILTRQSNKREYDNSRLTPDQKYQLQMLFMKTQSSLTIHDSKSDNRYLQHRLTEAMVI 215 Query: 229 ETGLKRMNDESADIFRSNESQKNKYRSGFSFEGQGTTGVKKHLLQGMITIVPSFNNEEAS 288 E K + E+ +FR N+ + +YR GF E G TG+KK +L+ + + ++ Sbjct: 216 EVANKARDLETIKMFRFNDQEVQQYRDGFGLEHSGVTGIKKFMLETLFLDRKETEKDPSN 275 Query: 289 -TKLAVDAARTAAEHSPAYALILSKDNSRTSQVKAGMLYSRLLLAGHEQGLVMQPLSQVL 347 +K +VD R+ + S +A I +K NSR QVK G Y RL L GL P+SQ+L Sbjct: 276 FSKQSVDITRSISGSSSTFAWITTKGNSRLQQVKVGRDYCRLNLTTTAMGLAQHPMSQIL 335 Query: 348 EEYPEMKAPYTRIHQEYAPGGE-TIQFLIRLGQPAQDTPLSMRREVTDLI 396 +EY +M + Q + E T+Q L+RLG+ A+ T + RR V+ L+ Sbjct: 336 QEYDDMLPLQAQFKQRFNIKAEQTVQMLVRLGK-AKKTTHTPRRLVSSLL 384 >Q28QE0.1 Q28QE0_JANSC Twin-arginine translocation pathway signal Length = 378 Score = 168 bits (425), Expect = 9e-40, Method: Compositional matrix adjust. Identities = 114/363 (31%), Positives = 170/363 (46%), Gaps = 24/363 (6%) Query: 41 LDPWKRDYVDQFKDPRVKLAAYGLLAPNGHNLQPWKIRLDKQNPLKFSLYTDSSRLALES 100 L PW + DPR+ +Y +LAPN HN QPW + LD + L +LY D+ R + Sbjct: 34 LAPWT--LAGSYSDPRMNALSYAILAPNPHNRQPWIVSLDGDDGL--TLYFDTERQLPHT 89 Query: 101 DPVARQTMVSQGTFLEYLRVGGEQLGYRAAIRLFPEGEYDEQKLSGSMDQKPVAEITLSK 160 DP RQ + G FLE +R+ GY + LFPEGE E +DQ+PVA T ++ Sbjct: 90 DPFDRQLTIGLGCFLELMRMAANANGYDVDVSLFPEGEDREH-----LDQRPVARATFAR 144 Query: 161 AQPAANPLFDYMFLPDTNRMAYK-----PDPLTAEQRQRLEQTNADGALKLIFLDAPEDI 215 A PAA+PLF ++ +N+ ++ P A T G+ + DI Sbjct: 145 ATPAADPLFAHVMDRRSNKEPFETARAVPANALARILTVARHTEIGGSTTEV------DI 198 Query: 216 KKLGEYSLEGTDIETGLKRMNDESADIFRSNESQKNKYRSGFSFEGQGTTGVKKHLLQGM 275 + S + IE ES D+ R +++ N G F G + G+ Sbjct: 199 AYWRQVSSQALTIEIETPDTYRESVDLMRIGKAEVNANPDGIDFSG---AMFETLAFAGV 255 Query: 276 ITIVPSFNNEEASTKLAVDAARTAAEHSPAYALILSKDNSRTSQVKAGMLYSRLLLAGHE 335 +T + + + A E + + ++++ N+R +Q+ AG + R+ LA Sbjct: 256 MTRDALLDTSSIGFQQGMAAVLANTETAMGHLWMVTQGNTRRNQIAAGGDWLRINLACTA 315 Query: 336 QGLVMQPLSQVLEEYPEMKAPYTRIHQEYAPGGETIQFLIRLGQPAQDTPLSMRREVTDL 395 +GL QPLSQ L+EYPEM A Y HQ AP G T+Q L R+G + P+S R + Sbjct: 316 EGLGFQPLSQALQEYPEMTALYDETHQRLAPNGGTVQMLARIGY-GPEVPVSPRWPIDAK 374 Query: 396 IAD 398 I D Sbjct: 375 IGD 377 >B5J893.1 B5J893_9RHOB Putative uncharacterized protein Length = 380 Score = 152 bits (384), Expect = 5e-35, Method: Compositional matrix adjust. Identities = 108/358 (30%), Positives = 164/358 (45%), Gaps = 35/358 (9%) Query: 43 PWKRDYVDQFKDPRVKLAAYGLLAPNGHNLQPWKIRLDKQNPLKFSLYTDSSRLALESDP 102 PW + Q+ DPR++ ++ +LAPN HN QPW + D P LY D RL +DP Sbjct: 38 PWTQ--AGQYDDPRMRALSWAILAPNPHNRQPWMV--DLSQPDTAVLYVDPDRLLPHTDP 93 Query: 103 VARQTMVSQGTFLEYLRVGGEQLGYRAAIRLFPEGEYDEQKLSGSMDQKPVAEITLSKAQ 162 RQ + G FLE LR+ Q G LFP+G + ++D +PVA +++ Sbjct: 94 FNRQITIGLGCFLEVLRMAALQDGLDVDFDLFPDGSDPD-----ALDTRPVAICRFAQSV 148 Query: 163 PAANPLFDYMFLPDTNRMAYKPDPLTAEQRQRLEQTNADGALKLIF-----------LDA 211 A +PLF A+ P+ + ++ + + D AL IF +A Sbjct: 149 AAPDPLF-----------AHVPNRRSLKEPFDITRAVPDAALASIFSAAIHGSVTSTTNA 197 Query: 212 PEDIKKLGEYSLEGTDIETGLKRMNDESADIFRSNESQKNKYRSGFSFEGQGTTGVKKHL 271 D+ ++ S E IE R ES D+FR + + G +F G G+ + Sbjct: 198 QVDVDEMRILSREALRIEIETPRTYKESVDLFRIGRYEVDADPDGIAFTGPMFEGLSR-- 255 Query: 272 LQGMITIVPSFNNEEASTKLAVDAARTAAEHSPAYALILSKDNSRTSQVKAGMLYSRLLL 331 GM + + + + + A + + Y ++ N+R Q++AG + R+ L Sbjct: 256 -AGMFNREIALDTSSTAYQQGLMAVYANTDTAMGYVWQVTPTNTREDQIRAGQDWVRINL 314 Query: 332 AGHEQGLVMQPLSQVLEEYPEMKAPYTRIHQEYAPGGETIQFLIRLGQPAQDTPLSMR 389 A G+ QPLSQ L+EY EM+ Y IH+ AP G T+Q RLG Q TP S R Sbjct: 315 AATALGIATQPLSQALQEYHEMQGSYADIHRRLAPEGGTVQMWARLGYGPQ-TPASPR 371 >A9CWU2.1 A9CWU2_9RHIZ Twin-arginine translocation pathway signal sequence domain protein Length = 386 Score = 147 bits (372), Expect = 1e-33, Method: Compositional matrix adjust. Identities = 109/350 (31%), Positives = 161/350 (46%), Gaps = 27/350 (7%) Query: 38 AKYLDPWKRDYVDQFKDPRVKLAAYGLLAPNGHNLQPWKIRLDKQNPLKFSLYTDSSRLA 97 ++ L PW+R + DPR++ +Y +LAPN HN QPW++ L ++ L D RL Sbjct: 35 SRALAPWQRAGGSTYADPRMRALSYAILAPNPHNRQPWQVELQGED--TVVLTVDLDRLL 92 Query: 98 LESDPVARQTMVSQGTFLEYLRVGGEQLGYRAAIRLFPEGEYDEQKLSGSMDQKPVAEIT 157 +DP +RQ + G FLE + + Q G+R I FP+G D ++L+ + PVA I Sbjct: 93 PHTDPFSRQITIGLGCFLELMVMAAAQTGHRVEIEPFPQGA-DSERLTTA----PVA-IA 146 Query: 158 LSKAQPAA--NPLFDYMFLPDTNRMAYK-----PDPLTAEQRQRLEQTNADGALKLIFLD 210 P +PLF ++ +N+ + PD A G D Sbjct: 147 RFVQDPGIRPDPLFAHVMARRSNKEPFDTARAVPDAALAAIVASARHGGRLGGTN----D 202 Query: 211 APE--DIKKLGEYSLEGTDIETGLKRMNDESADIFRSNESQKNKYRSGFSFEGQGTTGVK 268 A E + L E +L+ IE ES D+FR +++ N G F G + Sbjct: 203 AAEVASWRTLTETALQ---IEIDTPHTYKESVDLFRIGKAEVNANPDGIDFSG---PMFE 256 Query: 269 KHLLQGMITIVPSFNNEEASTKLAVDAARTAAEHSPAYALILSKDNSRTSQVKAGMLYSR 328 L G + + A K VDA + A+ +++ NSR Q+ AG + R Sbjct: 257 TLALFGQFNRELALDRTSAGFKQGVDAVLENTRTAMAHVWMVTDGNSRADQIAAGADWLR 316 Query: 329 LLLAGHEQGLVMQPLSQVLEEYPEMKAPYTRIHQEYAPGGETIQFLIRLG 378 LA QG+ QPLSQ L+E+PEM Y +H AP G T+Q L+RLG Sbjct: 317 ANLAATSQGIGFQPLSQALQEFPEMAELYRDVHARLAPNGGTVQMLVRLG 366 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.315 0.132 0.373 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,211,344,911 Number of extensions: 92588278 Number of successful extensions: 222879 Number of sequences better than 1.0e-05: 64 Number of HSP's gapped: 222884 Number of HSP's successfully gapped: 64 Length of query: 398 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 260 Effective length of database: 1,760,596,133 Effective search space: 457754994580 Effective search space used: 457754994580 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)