BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0263.0002.p (293 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C1I9J6.1 C1I9J6_9CLOT Acetyl esterase family enzyme 188 6e-46 A8ME37.1 A8ME37_CALMQ Putative esterase 173 2e-41 C0EFB9.1 C0EFB9_9CLOT Putative uncharacterized protein 168 5e-40 C4IC39.1 C4IC39_CLOBU Acetyl esterase family enzyme 168 7e-40 B1R1I2.1 B1R1I2_CLOBU Acetyl esterase family enzyme 168 7e-40 >C1I9J6.1 C1I9J6_9CLOT Acetyl esterase family enzyme Length = 275 Score = 188 bits (477), Expect = 6e-46, Method: Compositional matrix adjust. Identities = 105/296 (35%), Positives = 161/296 (54%), Gaps = 30/296 (10%) Query: 1 VIHQTIRL------LENSAATLTTYIQDDGEFDRKNIVRPAVLICPGGGYTVISQNEGEP 54 +I++ I+L L+ S A ++ YI ++ E N R +++CPGGGY + S E EP Sbjct: 1 MIYEKIKLKDISSKLKESNAVISIYIPENSEEININKKRETIIMCPGGGYVMTSDREAEP 60 Query: 55 VALAYARMGYHAFVLTYSVKIDNPFPAALLELAYAMAVIRERAEEWLVDKDNISVIGFSA 114 VAL + G++ VL YSV N FP AL+ELA + +R +++EW VDKD I V GFSA Sbjct: 61 VALKFVAEGFNVVVLRYSVA-PNKFPKALIELAATVDYVRSKSKEWNVDKDKIIVCGFSA 119 Query: 115 GGNLALSLGIYYNQPLLTGNTGLSADQIKPNQLLLGYPAVTLEPRSEKTPDFVIELMEKG 174 GG+LA SLG+ ++ +L ++ D IKPN ++L YP +T E KG Sbjct: 120 GGHLAGSLGVLWDNKILEEALEINKDNIKPNAMILCYPVITSG-----------EFAHKG 168 Query: 175 LMPDMRGPNIREILMGKENLTDEEKASLNLLHKIHSELPRTFIWGTYEDSVILPTDLFGL 234 ++ G N+ E+ E+ L+L + P TF+W T++D + + Sbjct: 169 SFDNLLGENVSEV----------EREKLSLEKLVSKNTPTTFLWHTFDDGAVPVQNSLFF 218 Query: 235 AEALFRHNVPCELHMFEKGPHGMSLGDETVKPPEQVQHL--HLSEWFNLSVKWLRQ 288 A +L +NVP ELH++ G HG+SL +E Q +H+ H WF L+V+W++ Sbjct: 219 ANSLASNNVPFELHIYPNGVHGLSLCEELTAMNGQSEHINQHAGTWFKLAVEWIKN 274 >A8ME37.1 A8ME37_CALMQ Putative esterase Length = 287 Score = 173 bits (439), Expect = 2e-41, Method: Compositional matrix adjust. Identities = 98/293 (33%), Positives = 155/293 (52%), Gaps = 16/293 (5%) Query: 4 QTIRLLENSAATLTTYIQDDG---EFDRKNIVRPAVLICPGGGYTVISQNEGEPVALAYA 60 + IRL ++ TLT+YI D + R RPA++ICPGGG+ S E EPVA + Sbjct: 4 EVIRLRDDRPVTLTSYILDTSPEISWGR----RPAIIICPGGGFVRTSDREAEPVASIFL 59 Query: 61 RMGYHAFVLTYSVK---IDNPFPAALLELAYAMAVIRERAEEWLVDKDNISVIGFSAGGN 117 GYHAFVL YS + + +P ++ELA A+ IR A++W +D I++IGFSAGG Sbjct: 60 SRGYHAFVLRYSTESMGVSKVYPDVVIELANAVVSIRRNADKWNIDPGRIAIIGFSAGGT 119 Query: 118 LALSLGIYYNQPLLTGNTGLSADQIKPNQLLLGYPAVTLEPRSEKTPDFVIELMEKGLMP 177 +A + +++ L+ D +KP+ ++L YP V +E T + G++ Sbjct: 120 VAALYSVNWHRDWLSKLVNAPKDTLKPSAVILAYPVVDFAVMNEVTKNNR-NTPAAGVLF 178 Query: 178 DMRGPNIREILMGKENLTDEEKASLNLLHKIHSELPRTFIWGTYEDSVILPTDLFGLAEA 237 M + +G T+++ L+ + + P TFIW T +D V+ + A Sbjct: 179 KMMS-----LALGSGKFTEDDLRELSATYHVDENTPPTFIWTTADDDVVPVESIISYVNA 233 Query: 238 LFRHNVPCELHMFEKGPHGMSLGDETVKPPEQVQHLHLSEWFNLSVKWLRQGV 290 L R+ VP E H+FEKG HG+SL D+T Q + +++W L++ WL + + Sbjct: 234 LARNKVPFEFHVFEKGVHGLSLADKTTASNPQHVNPPVAKWIELALSWLERHI 286 >C0EFB9.1 C0EFB9_9CLOT Putative uncharacterized protein Length = 277 Score = 168 bits (426), Expect = 5e-40, Method: Compositional matrix adjust. Identities = 103/277 (37%), Positives = 151/277 (54%), Gaps = 28/277 (10%) Query: 15 TLTTYIQDDG-EFDRKNIVRPAVLICPGGGYTVISQNEGEPVALAYARMGYHAFVLTYSV 73 L Y+ + G EF+ RPAV++CPGGGY S E EPVAL G +AFVL YSV Sbjct: 25 VLQAYVIERGPEFNVLQ-KRPAVILCPGGGYGFTSDREAEPVALRLVSAGINAFVLRYSV 83 Query: 74 KIDNPFPAALLELAYAMAVIRERAEEWLVDKDNISVIGFSAGGNLALSLGIYYNQPLLTG 133 +P ALLELA A+ ++R+ AE+W +D++ + V GFSAGG+LA +LG+ + QP L Sbjct: 84 APAR-YPCALLELARAVELVRQNAEKWHIDRNRVIVCGFSAGGHLAANLGVAWEQPFLCE 142 Query: 134 NTGLSADQIKPNQLLLGYPAVTLEPRSEKTPDFVIELMEKGLMPDMRGPNIREILMGKEN 193 G + ++PN ++L YP +T E G ++ G E LM Sbjct: 143 TLGAKSRDLRPNGMILSYPVITAG-----------EYAHIGSFENLLGDTKNEELM---- 187 Query: 194 LTDEEKASLNLLHKIHSELPRTFIWGTYEDSVILPTDLFGLAEALFRHNVPCELHMFEKG 253 AS++L ++ P +F+W T D V+ + A AL + VP ELH++E+G Sbjct: 188 ------ASVSLEKLVNRSTPPSFLWHTQADPVVPLENTVLFAMALRNNKVPFELHIYERG 241 Query: 254 PHGMSLGDET--VKPPEQVQHLHLSEWFNLSVKWLRQ 288 HG+SL DET V P V ++ W +++KWL++ Sbjct: 242 GHGLSLADETSAVLPRHIVP--DVTNWIEMAIKWLKR 276 >C4IC39.1 C4IC39_CLOBU Acetyl esterase family enzyme Length = 273 Score = 168 bits (425), Expect = 7e-40, Method: Compositional matrix adjust. Identities = 102/278 (36%), Positives = 150/278 (53%), Gaps = 28/278 (10%) Query: 14 ATLTTYIQDDG-EFDRKNIVRPAVLICPGGGYTVISQNEGEPVALAYARMGYHAFVLTYS 72 + L TYI D+ E K RP +L+CPGGGY + S EGE + + + MGYH +L YS Sbjct: 17 SKLCTYILDNSPEIGIKK--RPIILMCPGGGYCMTSDREGEILGMQFLSMGYHVAILRYS 74 Query: 73 VKIDNPFPAALLELAYAMAVIRERAEEWLVDKDNISVIGFSAGGNLALSLGIYYNQPLLT 132 + +P ALLELA AM +I E AEEW V+++NI V G SAGG+LA SLG+++++ L Sbjct: 75 CA-PSVYPTALLELAQAMKLIHEHAEEWHVEENNIIVQGASAGGHLAASLGVFWHEKWLA 133 Query: 133 GNTGLSADQIKPNQLLLGYPAVTLEPRSEKTPDFVIELMEKGLMPDMRGPNIREILMGKE 192 TG+ +Q+KP +LL YP +T + RG + +L G+ Sbjct: 134 DATGILCEQLKPAAMLLCYPVITSGKYAH------------------RG-SFESLLNGQ- 173 Query: 193 NLTDEEKASLNLLHKIHSELPRTFIWGTYEDSVILPTDLFGLAEALFRHNVPCELHMFEK 252 TDE ++L ++ ++ P+ FIW TY D + + L EA+ ++ +P E HM+ Sbjct: 174 -YTDEMLNKVSLEKQVTNDTPQAFIWHTYTDDCVPVQNSLLLIEAMKQYEIPVEFHMYPV 232 Query: 253 GPHGMSLGDETVKPPE--QVQHLHLSEWFNLSVKWLRQ 288 G HG+S DE P+ VQ W L+ WL+ Sbjct: 233 GGHGLSTCDELTATPDGYGVQE-ECQSWLPLAKSWLKH 269 >B1R1I2.1 B1R1I2_CLOBU Acetyl esterase family enzyme Length = 273 Score = 168 bits (425), Expect = 7e-40, Method: Compositional matrix adjust. Identities = 102/278 (36%), Positives = 150/278 (53%), Gaps = 28/278 (10%) Query: 14 ATLTTYIQDDG-EFDRKNIVRPAVLICPGGGYTVISQNEGEPVALAYARMGYHAFVLTYS 72 + L TYI D+ E K RP +L+CPGGGY + S EGE + + + MGYH +L YS Sbjct: 17 SKLCTYILDNSPEIGIKK--RPIILMCPGGGYCMTSDREGEILGMQFLSMGYHVAILRYS 74 Query: 73 VKIDNPFPAALLELAYAMAVIRERAEEWLVDKDNISVIGFSAGGNLALSLGIYYNQPLLT 132 + +P ALLELA AM +I E AEEW V+++NI V G SAGG+LA SLG+++++ L Sbjct: 75 CA-PSVYPTALLELAQAMKLIHEHAEEWHVEENNIIVQGASAGGHLAASLGVFWHEKWLA 133 Query: 133 GNTGLSADQIKPNQLLLGYPAVTLEPRSEKTPDFVIELMEKGLMPDMRGPNIREILMGKE 192 TG+ +Q+KP +LL YP +T + RG + +L G+ Sbjct: 134 DATGILCEQLKPAAMLLCYPVITSGKYAH------------------RG-SFESLLNGQ- 173 Query: 193 NLTDEEKASLNLLHKIHSELPRTFIWGTYEDSVILPTDLFGLAEALFRHNVPCELHMFEK 252 TDE ++L ++ ++ P+ FIW TY D + + L EA+ ++ +P E HM+ Sbjct: 174 -YTDEMLNKVSLEKQVTNDTPQAFIWHTYTDDCVPVQNSLLLIEAMKQYEIPVEFHMYPV 232 Query: 253 GPHGMSLGDETVKPPE--QVQHLHLSEWFNLSVKWLRQ 288 G HG+S DE P+ VQ W L+ WL+ Sbjct: 233 GGHGLSTCDELTATPDGYGVQE-ECQSWLPLAKSWLKH 269 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.137 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,887,776,085 Number of extensions: 82023836 Number of successful extensions: 180950 Number of sequences better than 1.0e-05: 337 Number of HSP's gapped: 180802 Number of HSP's successfully gapped: 361 Length of query: 293 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 159 Effective length of database: 1,798,280,193 Effective search space: 285926550687 Effective search space used: 285926550687 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)