BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0261.0007.p (377 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KFK5.1 B3KFK5_9BACI tRNA (5-methylaminomethyl-2-thiouridylate)... 592 e-167 B1DIX0.1 B1DIX0_9BACL tRNA (5-methylaminomethyl-2-thiouridylate)... 550 e-154 A8MEV9.1 MNMA_ALKOO tRNA-specific 2-thiouridylase mnmA EC=2.8.1.- 482 e-134 C0ZEA8.1 C0ZEA8_BREBN tRNA U34-sulfurtransferase 481 e-134 A6TUB2.1 MNMA_ALKMQ tRNA-specific 2-thiouridylase mnmA EC=2.8.1.- 471 e-131 >B3KFK5.1 B3KFK5_9BACI tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase EC=2.1.1.61 Length = 375 Score = 592 bits (1526), Expect = e-167, Method: Compositional matrix adjust. Identities = 288/372 (77%), Positives = 310/372 (83%), Gaps = 2/372 (0%) Query: 1 MVISMTKANQDTRXXXXXXXXXXXXXTALLLKQQGYDVIGIFMKNWDDTDEFGVCTAETD 60 MVI+M+K +TR TALLLKQQGY+VIGIFMKNWDDTDE G CTAE D Sbjct: 3 MVITMSKEKHNTRIVVGMSGGVDSSVTALLLKQQGYEVIGIFMKNWDDTDELGYCTAEAD 62 Query: 61 AEDVRRVCEQIDIPYYTVNFEKEYFDKVFSYFLDEYKAGRTPNPDVMCNREIKFGEFLNK 120 AEDVRRVCEQ+DIPYYTVNFEKEYFDKVFSYFLDEYKAGRTPNPDVMCNREIKFGEFLNK Sbjct: 63 AEDVRRVCEQLDIPYYTVNFEKEYFDKVFSYFLDEYKAGRTPNPDVMCNREIKFGEFLNK 122 Query: 121 ALQLGADYVATGHYARVIEEDDVFKLLRGVDSNKDQTYFLNALNQYQLSKAMFPIGHLPK 180 A+ LGADYVATGHYARVIEED ++LLRGVDSNKDQTYFLNAL+Q QL+KAMFPIGHLPK Sbjct: 123 AMDLGADYVATGHYARVIEEDGQYRLLRGVDSNKDQTYFLNALSQEQLAKAMFPIGHLPK 182 Query: 181 PEVRRIAEEAGLYTAKKKDSTGVCFIGERNFREFLSQYLPAQSGDMVDIATGEVKGRHEG 240 PEVRR+AEEAGLYTAKKKDSTGVCFIGERNF+EFL QYLPAQSGDMVDI TGEVKGRH+G Sbjct: 183 PEVRRLAEEAGLYTAKKKDSTGVCFIGERNFKEFLGQYLPAQSGDMVDIVTGEVKGRHDG 242 Query: 241 LMYYXXXXXXXXXXXXXXXXEPWFVAEKDLNRNILYVVQGDKHPSLYSTSLTASGVNWIE 300 LMYY EPWFVA+KDL NILYVVQGD HPSLYST L ASG+NWI Sbjct: 243 LMYYTLGQRQGLGIGGSGSGEPWFVADKDLTNNILYVVQGD-HPSLYSTGLVASGINWIA 301 Query: 301 -EGTLGTAPLKCTAKFRYRQPDQGVTLTAQEDGTVHVAFDVPQKAITPGQAVVFYLGEQC 359 + + CTAKFRYRQPDQ VT+T +EDGTVHVAF PQKAITPGQAVVFY G++C Sbjct: 302 GQDAMPKDAYTCTAKFRYRQPDQEVTITPREDGTVHVAFAKPQKAITPGQAVVFYQGDEC 361 Query: 360 LGGGTIEAAEKV 371 LGGGTIE A+KV Sbjct: 362 LGGGTIETADKV 373 >B1DIX0.1 B1DIX0_9BACL tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase EC=2.1.1.61 Length = 377 Score = 550 bits (1417), Expect = e-154, Method: Compositional matrix adjust. Identities = 271/372 (72%), Positives = 295/372 (79%), Gaps = 10/372 (2%) Query: 5 MTKANQDTRXXXXXXXXXXXXXTALLLKQQGYDVIGIFMKNWDDTDEFGVCTAETDAEDV 64 M K+ Q+TR TALLLKQQGYDV+GIFMKNWDDTDEFG CTAE DAEDV Sbjct: 1 MVKSKQETRVVVGMSGGVDSSVTALLLKQQGYDVVGIFMKNWDDTDEFGHCTAEEDAEDV 60 Query: 65 RRVCEQIDIPYYTVNFEKEYFDKVFSYFLDEYKAGRTPNPDVMCNREIKFGEFLNKALQL 124 RRVC+QI IPYYTVNFEK+YFDKVF+YFLDEYK GRTPNPDVMCNREIKFG+FL KA +L Sbjct: 61 RRVCDQIGIPYYTVNFEKQYFDKVFTYFLDEYKRGRTPNPDVMCNREIKFGDFLKKANEL 120 Query: 125 GADYVATGHYARV-IEEDDVFKLLRGVDSNKDQTYFLNALNQYQLSKAMFPIGHLPKPEV 183 GADY+ATGHYARV ED +LLRGVD NKDQTYFL+ALNQ QL KAMFPIGHLPKPEV Sbjct: 121 GADYLATGHYARVERNEDGETRLLRGVDGNKDQTYFLSALNQKQLEKAMFPIGHLPKPEV 180 Query: 184 RRIAEEAGLYTAKKKDSTGVCFIGERNFREFLSQYLPAQSGDMVDIATGEVKGRHEGLMY 243 RRIAEEAGLYTAKKKDSTGVCFIGERNF+EFLS YLPA+ GDMVDI +GEVKGRH+GLMY Sbjct: 181 RRIAEEAGLYTAKKKDSTGVCFIGERNFKEFLSGYLPARPGDMVDIRSGEVKGRHDGLMY 240 Query: 244 YXXXXXXXXXXXXXXXXEPWFVAEKDLNRNILYVVQGDKHPSLYSTSLTASGVNWIE--- 300 Y EPWFVA+KDL RNILYVVQG+ HPSLYST LTASG+NWI Sbjct: 241 YTLGQRQGLGIGGSGNGEPWFVADKDLERNILYVVQGESHPSLYSTGLTASGMNWIAPLR 300 Query: 301 -EGTLGTAPLKCTAKFRYRQPDQGVTLTAQEDGTVHVAFDVPQKAITPGQAVVFYLGEQC 359 EGT +CTAKFRYRQPDQGV++T DGT V FD PQKAITPGQAVV Y G+ C Sbjct: 301 PEGT-----FRCTAKFRYRQPDQGVSVTLHTDGTAEVVFDYPQKAITPGQAVVLYDGDVC 355 Query: 360 LGGGTIEAAEKV 371 L GGTI+ K+ Sbjct: 356 LAGGTIDIVHKI 367 >A8MEV9.1 MNMA_ALKOO tRNA-specific 2-thiouridylase mnmA EC=2.8.1.- Length = 364 Score = 482 bits (1240), Expect = e-134, Method: Compositional matrix adjust. Identities = 230/362 (63%), Positives = 276/362 (76%), Gaps = 2/362 (0%) Query: 5 MTKANQDTRXXXXXXXXXXXXXTALLLKQQGYDVIGIFMKNWDDTDEFGVCTAETDAEDV 64 M+KA +DT+ ALLLKQQGYDV+GIFMKNWD++DE G CT+ D EDV Sbjct: 1 MSKAPKDTKVVIGMSGGVDSSVAALLLKQQGYDVVGIFMKNWDESDELGYCTSAEDYEDV 60 Query: 65 RRVCEQIDIPYYTVNFEKEYFDKVFSYFLDEYKAGRTPNPDVMCNREIKFGEFLNKALQL 124 RRVC+QI IPYY+VNFEKEY+D+VF+YFL+EYKAGRTPNPDVMCN+EIKF FL+ A++L Sbjct: 61 RRVCDQIGIPYYSVNFEKEYWDRVFTYFLNEYKAGRTPNPDVMCNKEIKFKAFLDHAIKL 120 Query: 125 GADYVATGHYARVIEEDDVFKLLRGVDSNKDQTYFLNALNQYQLSKAMFPIGHLPKPEVR 184 GADY+ATGHYA+V D ++LLRGVD+NKDQTYFLN LNQYQLSKAMFP+GHL K ++R Sbjct: 121 GADYLATGHYAQVDYSDGEYRLLRGVDTNKDQTYFLNQLNQYQLSKAMFPVGHLQKKDLR 180 Query: 185 RIAEEAGLYTAKKKDSTGVCFIGERNFREFLSQYLPAQSGDMVDIATGEVKGRHEGLMYY 244 +IA +AGL TA KKDSTG+CFIGERNF+EFLS YLPA+ G ++ + GEVKG+H+GLMYY Sbjct: 181 QIALDAGLATASKKDSTGICFIGERNFKEFLSNYLPAKPGKIISL-DGEVKGKHDGLMYY 239 Query: 245 XXXXXXXXXXXXXXXXEPWFVAEKDLNRNILYVVQGDKHPSLYSTSLTASGVNWIEEGTL 304 EPWFV +KDL +NILYV QG+ HPSLYS L + + W+ E Sbjct: 240 TLGQRKGLGIGGAGTGEPWFVVDKDLEKNILYVTQGETHPSLYSRGLIGADLQWVSENPK 299 Query: 305 GTAPLKCTAKFRYRQPDQGVTLTAQEDGTVHVAFDVPQKAITPGQAVVFYLGEQCLGGGT 364 + +KCTAKFRYRQPDQGVT+ +E T V FD PQKAITPGQAVVFY G CLGG T Sbjct: 300 PSV-IKCTAKFRYRQPDQGVTVYVEEGNTCRVMFDEPQKAITPGQAVVFYDGVVCLGGAT 358 Query: 365 IE 366 I+ Sbjct: 359 ID 360 >C0ZEA8.1 C0ZEA8_BREBN tRNA U34-sulfurtransferase Length = 370 Score = 481 bits (1238), Expect = e-134, Method: Compositional matrix adjust. Identities = 240/367 (65%), Positives = 267/367 (72%), Gaps = 4/367 (1%) Query: 7 KANQDTRXXXXXXXXXXXXXTALLLKQQGYDVIGIFMKNWDDTDEFGVCTAETDAEDVRR 66 K +DTR TA LLKQQGYDVIGIFMKNWDDTDEFG CTAE D +DVRR Sbjct: 2 KRPEDTRVVVGMSGGVDSSVTAYLLKQQGYDVIGIFMKNWDDTDEFGHCTAEEDFQDVRR 61 Query: 67 VCEQIDIPYYTVNFEKEYFDKVFSYFLDEYKAGRTPNPDVMCNREIKFGEFLNKALQLGA 126 VCEQI IPYYTVNFEKEY DKVF YFLDEYK GRTPNPDVMCNREIKFGE L K + LGA Sbjct: 62 VCEQIGIPYYTVNFEKEYMDKVFQYFLDEYKRGRTPNPDVMCNREIKFGELLAKVMDLGA 121 Query: 127 DYVATGHYARVIEEDDVFKLLRGVDSNKDQTYFLNALNQYQLSKAMFPIGHLPKPEVRRI 186 DY+ATGHYA+V D +KL+RG D+NKDQTYFLN L Q QLSK MFPIGHLPKP+VR I Sbjct: 122 DYIATGHYAQVKYIDGEYKLIRGADNNKDQTYFLNVLGQEQLSKTMFPIGHLPKPQVREI 181 Query: 187 AEEAGLYTAKKKDSTGVCFIGERNFREFLSQYLPAQSGDMVDIATGEVKGRHEGLMYYX- 245 AE+AGLYTAKKKDSTG+CFIGERNFREFL YLPA+ G+ ++ G V G H+GLMYY Sbjct: 182 AEKAGLYTAKKKDSTGICFIGERNFREFLQNYLPAKQGN-IETVDGAVIGTHDGLMYYTL 240 Query: 246 -XXXXXXXXXXXXXXXEPWFVAEKDLNRNILYVVQGDKHPSLYSTSLTASGVNWIEEGTL 304 +PWFV +KDL RN L V +G H LYS SL A+ V+W+ + Sbjct: 241 GQRQGLGIGGGHGTSGQPWFVVDKDLERNALIVGEGSDHIRLYSKSLLATNVSWVSDKK- 299 Query: 305 GTAPLKCTAKFRYRQPDQGVTLTAQEDGTVHVAFDVPQKAITPGQAVVFYLGEQCLGGGT 364 + CTAKFRYRQPDQGVT+ EDG V V FD PQKA+TPGQAVVFY GE CLGGGT Sbjct: 300 PSETFTCTAKFRYRQPDQGVTVHLLEDGNVEVVFDQPQKAVTPGQAVVFYNGEVCLGGGT 359 Query: 365 IEAAEKV 371 I+ V Sbjct: 360 IDKVRLV 366 >A6TUB2.1 MNMA_ALKMQ tRNA-specific 2-thiouridylase mnmA EC=2.8.1.- Length = 369 Score = 471 bits (1212), Expect = e-131, Method: Compositional matrix adjust. Identities = 233/368 (63%), Positives = 268/368 (72%), Gaps = 2/368 (0%) Query: 5 MTKANQDTRXXXXXXXXXXXXXTALLLKQQGYDVIGIFMKNWDDTDEFGVCTAETDAEDV 64 M K +DT+ AL LK+QGYDVIGIFMKNWD+ DE+G CTA D EDV Sbjct: 1 MKKDPKDTKVVIGMSGGVDSSVAALKLKEQGYDVIGIFMKNWDEPDEYGYCTAAEDYEDV 60 Query: 65 RRVCEQIDIPYYTVNFEKEYFDKVFSYFLDEYKAGRTPNPDVMCNREIKFGEFLNKALQL 124 RRVCEQI IPYYTVNFEKEY++KVF YFLDEYK GRTPNPDVMCN+EIKF FL AL+L Sbjct: 61 RRVCEQIGIPYYTVNFEKEYWEKVFQYFLDEYKKGRTPNPDVMCNKEIKFKAFLEHALKL 120 Query: 125 GADYVATGHYARVIEEDDVFKLLRGVDSNKDQTYFLNALNQYQLSKAMFPIGHLPKPEVR 184 GADY+ATGHYA+V +D ++LLRGVDSNKDQTYFLN L QYQLSKAMFPIGH+ K +VR Sbjct: 121 GADYLATGHYAQVDYKDGEYRLLRGVDSNKDQTYFLNTLGQYQLSKAMFPIGHIDKKKVR 180 Query: 185 RIAEEAGLYTAKKKDSTGVCFIGERNFREFLSQYLPAQSGDMVDIATGEVKGRHEGLMYY 244 +A L TAKKKDSTG+CFIGERNF+EFLSQYLPAQ G+M D+ +G VKG+H+GLMY+ Sbjct: 181 EMALAHDLVTAKKKDSTGICFIGERNFKEFLSQYLPAQPGEMRDL-SGNVKGKHDGLMYH 239 Query: 245 XXXXXXXXXXXXXXXXEPWFVAEKDLNRNILYVVQGDKHPSLYSTSLTASGVNWIEEGTL 304 EPWFV KDL NILYVVQG+ HPSLY+ L A+ ++W+ E + Sbjct: 240 TLGQRKGLGIGGSGSGEPWFVVSKDLKNNILYVVQGEDHPSLYTYGLLATDLHWVSEKEI 299 Query: 305 GTAPLKCTAKFRYRQPDQGVTLTAQEDGTVHVAFDVPQKAITPGQAVVFYLGEQCLGGGT 364 CTAKFRYRQPDQ VT+ T V FD PQKA+TPGQAVVFY GE CLGGG Sbjct: 300 -PQTFTCTAKFRYRQPDQNVTVQLTGGDTCKVLFDGPQKAVTPGQAVVFYNGEACLGGGI 358 Query: 365 IEAAEKVV 372 I+ K V Sbjct: 359 IDETIKTV 366 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.137 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,129,065,020 Number of extensions: 87947266 Number of successful extensions: 186468 Number of sequences better than 1.0e-05: 1661 Number of HSP's gapped: 180907 Number of HSP's successfully gapped: 1695 Length of query: 377 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 240 Effective length of database: 1,770,017,148 Effective search space: 424804115520 Effective search space used: 424804115520 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)