BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0260.0033.p (289 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q03DB0.1 Q03DB0_PEDPA Permease of the major facilitator superfamily 259 2e-67 Q38XK6.1 Q38XK6_LACSS Putative drug:H(+) antiporter 257 1e-66 C2L2Q3.1 C2L2Q3_LACSK MFS family major facilitator transporter 254 4e-66 Q88ZH1.1 Q88ZH1_LACPL Transport protein 251 7e-65 C2FR63.1 C2FR63_LACPL MFS family major facilitator transporter 250 1e-64 >Q03DB0.1 Q03DB0_PEDPA Permease of the major facilitator superfamily Length = 480 Score = 259 bits (662), Expect = 2e-67, Method: Compositional matrix adjust. Identities = 123/247 (49%), Positives = 177/247 (71%) Query: 9 LPLLIASRVIQAIGASMTMANSQGIVTDIFPATERGKALGLIGTFVSLGSIAGPSLGGII 68 L L+ +R+IQA+GA+MTM+ + GI+T++FPA ERG+ALG+IG+FV+LGSIAGP +GG+I Sbjct: 110 LTFLLIARIIQAVGAAMTMSTNNGIITEVFPAQERGRALGMIGSFVALGSIAGPGIGGLI 169 Query: 69 VSTLGWEYIFWVNVPIGLIAIVLGWKVLPKDLIRVKSKIDLPGSLLFAVFIVTLFAGMLL 128 ++ W YIFW+NVPIGL+AI++G K+LPKD+I KID PG++LFA+ ++TLF G+ + Sbjct: 170 LAHFNWGYIFWINVPIGLLAILVGHKILPKDVIMTHQKIDKPGTILFAITMITLFVGISM 229 Query: 129 GQQVGYGDSRIISXXXXXXXXXXXXXXXEXXXXXXXXXXXXFKNPLFSLSILCAFLVFVA 188 GQ+VG+ I++ E FKN F+LSI+CAFL+F+ Sbjct: 230 GQEVGFTQPLILTMLFVALIALGTFLYVELHVETPILALYIFKNMRFTLSIICAFLIFIT 289 Query: 189 NFCFNIIAPFYAQNMLNLSPFYAGFLLMLFPISMVIVAPLSGALSDKIGSEFLTFAGLIV 248 F FN+I+PFY +N L L YAG+ LM++P+ V+ AP++GA+SDKIGSE LTF GL++ Sbjct: 290 GFFFNVISPFYLENTLGLPANYAGYTLMIYPLINVVTAPVAGAISDKIGSEKLTFVGLLI 349 Query: 249 MVIAQFG 255 ++ AQ G Sbjct: 350 ILAAQLG 356 >Q38XK6.1 Q38XK6_LACSS Putative drug:H(+) antiporter Length = 475 Score = 257 bits (656), Expect = 1e-66, Method: Compositional matrix adjust. Identities = 126/247 (51%), Positives = 174/247 (70%) Query: 9 LPLLIASRVIQAIGASMTMANSQGIVTDIFPATERGKALGLIGTFVSLGSIAGPSLGGII 68 LPLL+ +RV+QA+GASMTM+N+ GI+T++FP ERG+ALGL G+FV+LGSIAGP +GG+I Sbjct: 105 LPLLLGARVVQALGASMTMSNNNGIITEVFPLKERGRALGLTGSFVALGSIAGPGIGGLI 164 Query: 69 VSTLGWEYIFWVNVPIGLIAIVLGWKVLPKDLIRVKSKIDLPGSLLFAVFIVTLFAGMLL 128 ++ W YIFW+NVPIG++ I+ G +LPKD+I+ KID G + FA IV+LF G+ + Sbjct: 165 LAHFSWGYIFWINVPIGILTIIFGHFILPKDIIKTHEKIDYAGFVSFAALIVSLFLGIFI 224 Query: 129 GQQVGYGDSRIISXXXXXXXXXXXXXXXEXXXXXXXXXXXXFKNPLFSLSILCAFLVFVA 188 GQQ+G+ I++ E FKN FSLS+LCAFL+F+ Sbjct: 225 GQQIGFTSPIILTAFVVALISAGIFVWIEQHVEQPLLSFRLFKNVDFSLSLLCAFLIFIV 284 Query: 189 NFCFNIIAPFYAQNMLNLSPFYAGFLLMLFPISMVIVAPLSGALSDKIGSEFLTFAGLIV 248 NF FN+I+PFY +N L L+P AG+ LM+FPI V+VAP++G++SDKIG E LTF GLI+ Sbjct: 285 NFFFNVISPFYLENALGLAPNLAGYSLMIFPIVQVVVAPIAGSISDKIGPELLTFVGLIL 344 Query: 249 MVIAQFG 255 + I+Q G Sbjct: 345 ISISQVG 351 >C2L2Q3.1 C2L2Q3_LACSK MFS family major facilitator transporter Length = 483 Score = 254 bits (650), Expect = 4e-66, Method: Compositional matrix adjust. Identities = 126/247 (51%), Positives = 174/247 (70%) Query: 9 LPLLIASRVIQAIGASMTMANSQGIVTDIFPATERGKALGLIGTFVSLGSIAGPSLGGII 68 LPLL+ +RV+QA+GASMTM+N+ GI+T++FP ERG+ALGL G+FV+LGSIAGP +GG+I Sbjct: 105 LPLLLGARVVQALGASMTMSNNNGIITEVFPLKERGRALGLTGSFVALGSIAGPGIGGLI 164 Query: 69 VSTLGWEYIFWVNVPIGLIAIVLGWKVLPKDLIRVKSKIDLPGSLLFAVFIVTLFAGMLL 128 ++ W YIFW+NVPIG++ I+ G +LPKD+I+ KID G + FA IV+LF G+ + Sbjct: 165 LTHFSWGYIFWINVPIGILTIIFGHFILPKDIIKTHEKIDSAGFVSFAALIVSLFLGIFI 224 Query: 129 GQQVGYGDSRIISXXXXXXXXXXXXXXXEXXXXXXXXXXXXFKNPLFSLSILCAFLVFVA 188 GQQ+G+ I++ E FKN FSLS+LCAFL+F+ Sbjct: 225 GQQIGFISPIILTAFVVALISAGIFVWIEQHVEQPLLSFRLFKNIDFSLSLLCAFLIFIV 284 Query: 189 NFCFNIIAPFYAQNMLNLSPFYAGFLLMLFPISMVIVAPLSGALSDKIGSEFLTFAGLIV 248 NF FN+I+PFY +N L L+P AG+ LM+FPI V+VAP++G++SDKIG E LTF GLI+ Sbjct: 285 NFFFNVISPFYLENALGLAPNLAGYSLMIFPIVQVVVAPIAGSISDKIGPELLTFVGLIL 344 Query: 249 MVIAQFG 255 + I+Q G Sbjct: 345 ISISQVG 351 >Q88ZH1.1 Q88ZH1_LACPL Transport protein Length = 481 Score = 251 bits (640), Expect = 7e-65, Method: Compositional matrix adjust. Identities = 122/247 (49%), Positives = 173/247 (70%) Query: 9 LPLLIASRVIQAIGASMTMANSQGIVTDIFPATERGKALGLIGTFVSLGSIAGPSLGGII 68 LP L+ +R IQA+GA+MTM+ + GI+T++FP ERG+ALG+IG+FV+LGSIAGP +GG+I Sbjct: 106 LPFLLIARSIQAVGAAMTMSTNNGIITEVFPFKERGRALGMIGSFVALGSIAGPGIGGLI 165 Query: 69 VSTLGWEYIFWVNVPIGLIAIVLGWKVLPKDLIRVKSKIDLPGSLLFAVFIVTLFAGMLL 128 ++ L W YIFW+NVP+G++A++LG +LPKD+ + +D G+ LFAV +V LFAG+ + Sbjct: 166 LAHLSWGYIFWINVPVGILAMILGAMILPKDVTTTQQPLDKTGAGLFAVLMVALFAGVFI 225 Query: 129 GQQVGYGDSRIISXXXXXXXXXXXXXXXEXXXXXXXXXXXXFKNPLFSLSILCAFLVFVA 188 GQQ+G+ I++ E FKN FS+SILCA L+F+A Sbjct: 226 GQQIGFLQPVILALFAVAIVTLIIFVRVELRHENPLLALQLFKNARFSISILCALLIFIA 285 Query: 189 NFCFNIIAPFYAQNMLNLSPFYAGFLLMLFPISMVIVAPLSGALSDKIGSEFLTFAGLIV 248 NF FN+IAPFY +N L+ YAG+ LM+FPI V+VAP++G +SDKIG E LTF GL++ Sbjct: 286 NFFFNVIAPFYLENARGLAANYAGYALMVFPIVQVVVAPIAGTISDKIGPELLTFVGLVL 345 Query: 249 MVIAQFG 255 + I+Q G Sbjct: 346 ISISQVG 352 >C2FR63.1 C2FR63_LACPL MFS family major facilitator transporter Length = 481 Score = 250 bits (638), Expect = 1e-64, Method: Compositional matrix adjust. Identities = 121/247 (48%), Positives = 173/247 (70%) Query: 9 LPLLIASRVIQAIGASMTMANSQGIVTDIFPATERGKALGLIGTFVSLGSIAGPSLGGII 68 LP L+ +R IQA+GA+MTM+ + GI+T++FP ERG+ALG+IG+FV+LGSIAGP +GG+I Sbjct: 106 LPFLLIARSIQAVGAAMTMSTNNGIITEVFPFKERGRALGMIGSFVALGSIAGPGIGGLI 165 Query: 69 VSTLGWEYIFWVNVPIGLIAIVLGWKVLPKDLIRVKSKIDLPGSLLFAVFIVTLFAGMLL 128 ++ L W YIFW+NVP+G++A++LG +LPKD+ + +D G+ LFAV +V LFAG+ + Sbjct: 166 LAHLSWGYIFWINVPVGILAMILGAMILPKDVTTTQQPLDKTGAGLFAVLMVALFAGVFI 225 Query: 129 GQQVGYGDSRIISXXXXXXXXXXXXXXXEXXXXXXXXXXXXFKNPLFSLSILCAFLVFVA 188 GQQ+G+ I++ E FKN FS+S+LCA L+F+A Sbjct: 226 GQQIGFLQPVILALFAVAIVTLIIFVRVELRHENPLLALQLFKNARFSISVLCALLIFIA 285 Query: 189 NFCFNIIAPFYAQNMLNLSPFYAGFLLMLFPISMVIVAPLSGALSDKIGSEFLTFAGLIV 248 NF FN+IAPFY +N L+ YAG+ LM+FPI V+VAP++G +SDKIG E LTF GL++ Sbjct: 286 NFFFNVIAPFYLENARGLAANYAGYALMVFPIVQVVVAPIAGTISDKIGPELLTFVGLVL 345 Query: 249 MVIAQFG 255 + I+Q G Sbjct: 346 ISISQVG 352 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.328 0.144 0.428 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,428,905,029 Number of extensions: 54672748 Number of successful extensions: 276548 Number of sequences better than 1.0e-05: 6968 Number of HSP's gapped: 273684 Number of HSP's successfully gapped: 7031 Length of query: 289 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 155 Effective length of database: 1,798,280,193 Effective search space: 278733429915 Effective search space used: 278733429915 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 130 (54.7 bits)