BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0260.0030.p (386 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DCR6.1 B1DCR6_9BACL Major facilitator superfamily MFS_1 442 e-122 C0ZDD7.1 C0ZDD7_BREBN Putative transporter 434 e-120 B3K8C9.1 B3K8C9_9BACI Major facilitator superfamily MFS_1 417 e-114 A8FIK1.1 A8FIK1_BACP2 MFS family major facilitator transporter 346 3e-93 B4AJG8.1 B4AJG8_BACPU Inner membrane transport protein YdhP 344 9e-93 >B1DCR6.1 B1DCR6_9BACL Major facilitator superfamily MFS_1 Length = 405 Score = 442 bits (1136), Expect = e-122, Method: Compositional matrix adjust. Identities = 212/347 (61%), Positives = 284/347 (81%) Query: 1 LGAFAIGMTEFIIMGLLPNVARDLGVSIPQAGQLITSYALGVAIGAPVLTIFTHRLPQKK 60 +GAFAIGMTEF+IMG+LPNVA DL VSI AGQLIT YALGVA+GAP++TI T R+PQKK Sbjct: 31 VGAFAIGMTEFVIMGILPNVASDLNVSISAAGQLITMYALGVAVGAPIMTILTSRIPQKK 90 Query: 61 LLMILMCIFIFGNAVSVIAPTYTLLILARILTAFAHGTFLGVGTIIAARLVRPEKRAGAV 120 LL ILM +FI GN +SV+AP Y +L++ARI+TA HGTF GVG +IA++LVR +K+AGAV Sbjct: 91 LLCILMLLFILGNGLSVVAPNYAVLMIARIITALTHGTFFGVGAVIASKLVRADKQAGAV 150 Query: 121 SVVLAGLTVANIIGVPFGTFIGQQLGWRASFGAITVLGVVSLFGIIRFIPVIVQEQTANL 180 S+++AGLT+ANIIGVP GT+IGQ GWRASFGAI V+GV++L GII FIP + + ++ Sbjct: 151 SIMMAGLTIANIIGVPIGTYIGQNWGWRASFGAIAVMGVLALIGIIIFIPKLKLSKQVSV 210 Query: 181 SEQVRGLLNPKVLLILLTGALGCASLFSLFTYITPMLEEITGFAEHNVTWILVLFGAGVT 240 +Q+ + PK+L+ LL ALG A LF++FTYITP+L +ITGFAEH+VTWIL+LFG GVT Sbjct: 211 LKQISAIARPKLLVYLLIAALGNAGLFAVFTYITPLLTDITGFAEHSVTWILILFGIGVT 270 Query: 241 LGNLIGGKLADWKLMPSLIVNFAILAIMISLFSWTLHNPVLTIINVFMWGIAAFGIMPGI 300 +GN++GGKLADWKLMPS++ + +A+++++F++T+HN + +I +F+WG A+F + PG+ Sbjct: 271 VGNMVGGKLADWKLMPSILGLYLTIAVVLTIFTFTVHNEIAAVITIFLWGAASFAVFPGL 330 Query: 301 QLRIMNLAHKAPLLATTSSHSALNLGNATGAYLGGAAITQWGLSSIP 347 Q+RIM+LA AP LA+TSSHSA NLGNA+GA++GG IT L ++P Sbjct: 331 QVRIMSLAQSAPALASTSSHSAGNLGNASGAFIGGWVITHLSLGALP 377 >C0ZDD7.1 C0ZDD7_BREBN Putative transporter Length = 399 Score = 434 bits (1117), Expect = e-120, Method: Compositional matrix adjust. Identities = 215/374 (57%), Positives = 291/374 (77%) Query: 1 LGAFAIGMTEFIIMGLLPNVARDLGVSIPQAGQLITSYALGVAIGAPVLTIFTHRLPQKK 60 +G+FAIGMTEF+IMGLLPNVA DL VSI AGQLIT YALGVA+GAP+LT+ THR+PQKK Sbjct: 26 VGSFAIGMTEFVIMGLLPNVAEDLHVSISSAGQLITMYALGVAVGAPILTVLTHRIPQKK 85 Query: 61 LLMILMCIFIFGNAVSVIAPTYTLLILARILTAFAHGTFLGVGTIIAARLVRPEKRAGAV 120 LL +LM +FI GN +SV AP Y +L+ AR++TA HGTF GVG ++A+ LV P+KRA AV Sbjct: 86 LLCLLMILFILGNGISVFAPNYDILMGARLITALTHGTFFGVGAVVASSLVSPDKRAAAV 145 Query: 121 SVVLAGLTVANIIGVPFGTFIGQQLGWRASFGAITVLGVVSLFGIIRFIPVIVQEQTANL 180 S+++AGLT+ANIIGVP GTFIGQ +GWRASFG I ++G+++L GI+ F+P + QE T ++ Sbjct: 146 SIMMAGLTIANIIGVPLGTFIGQHMGWRASFGTIAIMGMIALVGILVFVPNMRQENTGSI 205 Query: 181 SEQVRGLLNPKVLLILLTGALGCASLFSLFTYITPMLEEITGFAEHNVTWILVLFGAGVT 240 + Q+ LL PK+LL LL GALG A LF++FTYITP+L +ITGFAE++VTWILVLFG GVT Sbjct: 206 TGQITALLKPKLLLYLLIGALGNAGLFTVFTYITPLLTQITGFAEYHVTWILVLFGCGVT 265 Query: 241 LGNLIGGKLADWKLMPSLIVNFAILAIMISLFSWTLHNPVLTIINVFMWGIAAFGIMPGI 300 +GN++GGKLADWKLMPS++ + + I+++LF++T+++P+ ++ +F+WG A+F + PG+ Sbjct: 266 IGNIVGGKLADWKLMPSILGLYFTICIILTLFTFTMYSPIAAVLTIFLWGAASFAVFPGL 325 Query: 301 QLRIMNLAHKAPLLATTSSHSALNLGNATGAYLGGAAITQWGLSSIPXXXXXXXXXXXXX 360 Q+R+MNLA AP LA+TSSHSA NLGNA GA++GG IT ++S+P Sbjct: 326 QVRVMNLAQNAPALASTSSHSAGNLGNAAGAFIGGWVITHLDITSLPWVGAVLVALGLIL 385 Query: 361 XXXXYLSTRSRQAA 374 Y++ R Q + Sbjct: 386 GIASYMAERKLQQS 399 >B3K8C9.1 B3K8C9_9BACI Major facilitator superfamily MFS_1 Length = 401 Score = 417 bits (1072), Expect = e-114, Method: Compositional matrix adjust. Identities = 210/347 (60%), Positives = 285/347 (82%) Query: 1 LGAFAIGMTEFIIMGLLPNVARDLGVSIPQAGQLITSYALGVAIGAPVLTIFTHRLPQKK 60 +GAFAIGMTEF+IMG+LPNVA DL VSI AGQLIT YALGVA+GAP+LT+ T+++PQKK Sbjct: 26 VGAFAIGMTEFVIMGILPNVADDLNVSIASAGQLITMYALGVAVGAPILTVLTNQIPQKK 85 Query: 61 LLMILMCIFIFGNAVSVIAPTYTLLILARILTAFAHGTFLGVGTIIAARLVRPEKRAGAV 120 LL++LM +FI GN +SV AP Y +L+ AR+LTA HGTF GVG +IA+ LVRP+KRAGAV Sbjct: 86 LLLLLMGLFILGNGISVFAPNYAVLMGARMLTALTHGTFFGVGAVIASNLVRPDKRAGAV 145 Query: 121 SVVLAGLTVANIIGVPFGTFIGQQLGWRASFGAITVLGVVSLFGIIRFIPVIVQEQTANL 180 S+++AGLT+ANIIGVP GTFIGQ +GWRASFGAI V+GV++L GI+ FIP I QEQ ++ Sbjct: 146 SIMMAGLTIANIIGVPIGTFIGQTMGWRASFGAIAVMGVLALIGILIFIPKIRQEQATSI 205 Query: 181 SEQVRGLLNPKVLLILLTGALGCASLFSLFTYITPMLEEITGFAEHNVTWILVLFGAGVT 240 +Q+ ++ P++LL LL GALG A LF++FTYI P+L EITGFAE +VTWIL+LFG GVT Sbjct: 206 VQQITAIIRPQLLLFLLIGALGNAGLFAVFTYIAPLLIEITGFAEPSVTWILILFGIGVT 265 Query: 241 LGNLIGGKLADWKLMPSLIVNFAILAIMISLFSWTLHNPVLTIINVFMWGIAAFGIMPGI 300 +GN++GG+LADWKL+PS++ + A+++++ + T+H+PV ++ + +WG A+F +MPG+ Sbjct: 266 VGNIVGGRLADWKLLPSILGVYFATAVILTILTATIHHPVTAVLTIVLWGAASFAVMPGM 325 Query: 301 QLRIMNLAHKAPLLATTSSHSALNLGNATGAYLGGAAITQWGLSSIP 347 Q+R+M+LA AP LA+T++HSA NLGNA GA++GG IT L+++P Sbjct: 326 QVRVMSLAQSAPALASTATHSAGNLGNAAGAFIGGLVITHLSLTALP 372 >A8FIK1.1 A8FIK1_BACP2 MFS family major facilitator transporter Length = 386 Score = 346 bits (887), Expect = 3e-93, Method: Compositional matrix adjust. Identities = 184/327 (56%), Positives = 247/327 (75%) Query: 1 LGAFAIGMTEFIIMGLLPNVARDLGVSIPQAGQLITSYALGVAIGAPVLTIFTHRLPQKK 60 LGAF+IGMTEF+IMGLLPNVA DL VSI +GQLIT YALGVA+G P+LT+ T ++ +K+ Sbjct: 13 LGAFSIGMTEFVIMGLLPNVANDLHVSISASGQLITGYALGVAVGGPILTLSTGKMSRKR 72 Query: 61 LLMILMCIFIFGNAVSVIAPTYTLLILARILTAFAHGTFLGVGTIIAARLVRPEKRAGAV 120 LL++LM +F+ GNAV+ ++ +Y LL+ ARILT+FAHGTF GVG I+AARLV EK A A+ Sbjct: 73 LLILLMILFVAGNAVAAVSTSYGLLMAARILTSFAHGTFFGVGAIMAARLVPKEKSASAI 132 Query: 121 SVVLAGLTVANIIGVPFGTFIGQQLGWRASFGAITVLGVVSLFGIIRFIPVIVQEQTANL 180 S + GLTVAN++GVPFGTFIG Q GWR SF I V+G++SL GII +P +E+ ++ Sbjct: 133 SFMFTGLTVANVLGVPFGTFIGNQFGWRMSFLVIAVIGLISLIGIISLVPNQSKEEVLDI 192 Query: 181 SEQVRGLLNPKVLLILLTGALGCASLFSLFTYITPMLEEITGFAEHNVTWILVLFGAGVT 240 +V L P+VL+ G A +F+ FTYI+P+L ++TGF E+ VT ILVLFG GVT Sbjct: 193 RNEVSVLKKPQVLVSFAMTIFGFAGVFTAFTYISPILLQVTGFQENGVTLILVLFGLGVT 252 Query: 241 LGNLIGGKLADWKLMPSLIVNFAILAIMISLFSWTLHNPVLTIINVFMWGIAAFGIMPGI 300 +GNL+GGKLADWKLMPS+IV+ +L ++ LF+ T V ++ +F+WGI +F ++P + Sbjct: 253 IGNLVGGKLADWKLMPSIIVSLILLIAVLLLFTITSEFKVPAVLTIFVWGIISFILVPTL 312 Query: 301 QLRIMNLAHKAPLLATTSSHSALNLGN 327 Q R MN+AH+AP LA+T SHSA N+GN Sbjct: 313 QYRTMNMAHEAPTLASTLSHSAFNIGN 339 >B4AJG8.1 B4AJG8_BACPU Inner membrane transport protein YdhP Length = 386 Score = 344 bits (882), Expect = 9e-93, Method: Compositional matrix adjust. Identities = 183/327 (55%), Positives = 246/327 (75%) Query: 1 LGAFAIGMTEFIIMGLLPNVARDLGVSIPQAGQLITSYALGVAIGAPVLTIFTHRLPQKK 60 LGAF+IGMTEF+IMGLLPNVA DL VSI +GQLIT YALGVA+G P+LT+ T ++ +K+ Sbjct: 13 LGAFSIGMTEFVIMGLLPNVANDLHVSISASGQLITGYALGVAVGGPILTLSTGKMSRKR 72 Query: 61 LLMILMCIFIFGNAVSVIAPTYTLLILARILTAFAHGTFLGVGTIIAARLVRPEKRAGAV 120 LL++LM +F+ GNAV+ ++ +Y LL+ ARILT+FAHGTF GVG I+ ARLV EK A A+ Sbjct: 73 LLILLMILFVAGNAVAAVSTSYGLLMAARILTSFAHGTFFGVGAIMVARLVPKEKSASAI 132 Query: 121 SVVLAGLTVANIIGVPFGTFIGQQLGWRASFGAITVLGVVSLFGIIRFIPVIVQEQTANL 180 S + GLTVAN++GVPFGTFIG Q GWR SF I V+G++SL GII +P +E+ ++ Sbjct: 133 SFMFTGLTVANVLGVPFGTFIGNQFGWRMSFLVIAVIGLISLIGIISLVPNQSKEEVLDI 192 Query: 181 SEQVRGLLNPKVLLILLTGALGCASLFSLFTYITPMLEEITGFAEHNVTWILVLFGAGVT 240 +V L P+VL+ G A +F+ FTYI+P+L ++TGF E+ VT ILVLFG GVT Sbjct: 193 RNEVSVLKKPQVLVSFAMTIFGFAGVFTAFTYISPILLQVTGFQENGVTLILVLFGLGVT 252 Query: 241 LGNLIGGKLADWKLMPSLIVNFAILAIMISLFSWTLHNPVLTIINVFMWGIAAFGIMPGI 300 +GNL+GGKLADWKLMPS+IV+ +L ++ LF+ T V ++ +F+WGI +F ++P + Sbjct: 253 IGNLVGGKLADWKLMPSIIVSLILLMAVLLLFTITSEFKVPAVMTIFVWGIISFILVPTL 312 Query: 301 QLRIMNLAHKAPLLATTSSHSALNLGN 327 Q R MN+AH+AP LA+T SHSA N+GN Sbjct: 313 QYRTMNMAHEAPTLASTLSHSAFNIGN 339 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.327 0.141 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,056,791,468 Number of extensions: 85021064 Number of successful extensions: 390176 Number of sequences better than 1.0e-05: 6419 Number of HSP's gapped: 389998 Number of HSP's successfully gapped: 6421 Length of query: 386 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 249 Effective length of database: 1,770,017,148 Effective search space: 440734269852 Effective search space used: 440734269852 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)