BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0260.0029.p (565 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1D9Q5.1 B1D9Q5_9BACL Extracellular solute-binding protein family 1 409 e-112 B3KEX3.1 B3KEX3_9BACI Extracellular solute-binding protein family 1 366 3e-99 A7VHE6.1 A7VHE6_9CLOT Putative uncharacterized protein 319 6e-85 A8SQM2.1 A8SQM2_9FIRM Putative uncharacterized protein 306 3e-81 C4Z3A7.1 C4Z3A7_9FIRM Multiple sugar transport system substrate-... 288 9e-76 >B1D9Q5.1 B1D9Q5_9BACL Extracellular solute-binding protein family 1 Length = 543 Score = 409 bits (1051), Expect = e-112, Method: Compositional matrix adjust. Identities = 216/517 (41%), Positives = 314/517 (60%), Gaps = 31/517 (5%) Query: 45 YGRYEEPVVMRIGFKVP-DSRLNTGDSNDNNPISRYLESLTNIKVIHSWEAKGEEPFTQK 103 Y E V + IG + D +L +G + ++N ++RY N+K + W+A G+ +TQK Sbjct: 48 YAPMSETVTISIGKEAAVDPKLPSGSTIEDNELTRYFAEKLNVKYTNEWQATGD-AYTQK 106 Query: 104 VQLAIDSNDLPDAMVVDRDQLEKLIDSDMIADLTQTYKEFGSELIKDMYDSTQGEALGDA 163 V L + + D+PD M+VD+ QL +L+++D+I DLT TYK F S +K YDST G +L A Sbjct: 107 VGLTLSTGDIPDVMIVDKQQLVQLVNADLIEDLTSTYKSFVSPSLKAAYDSTNGYSLASA 166 Query: 164 TRNGRLYGLPNVAIHADSPSLLWVRQDWLXXXXXPAPRSFEDIEAIAKAFIEQDPDGNGK 223 T +G+L PNV+ AD+ +++WVRQDWL AP++ +D+ + KAF++ K Sbjct: 167 TFDGKLMAFPNVSPGADAQNMVWVRQDWLDELQLEAPKTMDDVINVTKAFMD-------K 219 Query: 224 RDTVGLSAYNNIVYGTKPHVNGLDTLFSALHAFPTNWIRDSRGNVVYGSITPETKEALGK 283 + +VGL N ++ V G D++FSA +A+P W +D+ GN VYGSI PE KEAL K Sbjct: 220 KGSVGLLG-NQVIVNIGTSVFGFDSIFSAYNAYPQLWTKDASGNTVYGSIQPEVKEALAK 278 Query: 284 LAGWYKRGLIDPDFALYKETQ--EPIISGKAGMFFGPWWMPYYPLSEAVALDTRAEWRAY 341 + GW + G+ID +FA+ K Q E ++SGKAG+FFGPWW+P+ L+++VA D++AEW +Y Sbjct: 279 IRGWVQEGVIDKEFAVKKNEQVNELVVSGKAGIFFGPWWLPFGVLADSVAQDSKAEWVSY 338 Query: 342 AAPLDASGKFVTHMAPVTDRYLVVRKGYEHPEAVVKLLNVFTRLERRRDPNLEAVRSLDD 401 APLD GK THMA T RYLVV+KG++HPEAV+K LN+ D Sbjct: 339 LAPLDQEGKMNTHMASPTTRYLVVKKGFKHPEAVLKTLNM--------------QFDTDQ 384 Query: 402 FSAQTGIQPRAY----YPFDLLIDYADAIEKHYADMQQALHGKIDPDSLDPDTHLLYDLW 457 + +Q AY PF LL+ D EK +Q + GK D+L + +Y+ + Sbjct: 385 LQGEGIVQKDAYNWMNMPFSLLLSNYDDKEKKAKLVQDVIDGKAGADTLQGEAKQIYNSY 444 Query: 458 LADEEQPKKNLEGWKAANAYKYG-VSVLSTTAIDRVRSVFYGSTLHMRSNWPELQKLESE 516 L D+E PKK+L W +A+ G + ++ I+R V+Y T MR+ W L+KLE E Sbjct: 445 LKDKENPKKDLAAWSQRHAFLTGALPLIQDDKINRNVGVYYDQTDTMRTKWANLRKLEDE 504 Query: 517 TFLNIIVGDSPLSDFDLFAAKWRSLGGDAITQEVAKA 553 TFL II+G+ P+ FD F KW+S GGD IT+EVA+A Sbjct: 505 TFLKIIMGNEPVDSFDSFVGKWKSQGGDEITKEVAEA 541 >B3KEX3.1 B3KEX3_9BACI Extracellular solute-binding protein family 1 Length = 566 Score = 366 bits (940), Expect = 3e-99, Method: Compositional matrix adjust. Identities = 217/557 (38%), Positives = 307/557 (55%), Gaps = 45/557 (8%) Query: 24 NAPAAGSGSADIRAIKAESA-AYGRYEEPVVMRIGFKV--PDSRLNTGDSNDNNPISRYL 80 N PAA SA+ +E A +GRYEEPV + I ++ D+ L GD+ +N +R + Sbjct: 27 NTPAAPEKSAE----SSEPADPFGRYEEPVQISISIEIDPTDNELPPGDTPLDNQYTRQI 82 Query: 81 ESLTNIKVIHSWEAKGEEPFTQKVQLAIDSNDLPDAMVVDRDQLEKLIDSDMIADLTQTY 140 + N+ V H + A G+ Q++ LAI SNDLPDAMVV+ +L +L+++D IADLT+ Y Sbjct: 83 KEALNVDVDHQFAASGQN-LRQRISLAIASNDLPDAMVVNAVELRQLVEADQIADLTEVY 141 Query: 141 KEFGSELIKDMYDSTQGEALGDATRNGRLYGLPNVAIHADSPSLLWVRQDWLXXXXXPAP 200 +++ S IK + ++T G AL T +G++ LP V +AD LLW+RQDWL P Sbjct: 142 EQYASPDIKTIIENTNGAALDAVTFDGKIMALPGVQANADGIHLLWIRQDWLDKLNLEPP 201 Query: 201 RSFEDIEAIAKAFIEQDPDGNGKRDTVGLSA-------YNNIVYGTKPHVNGLDTLFSAL 253 + ++E +A+AF+E DPDGNG+RDT+G++ Y N + +K ++ G D +FSA Sbjct: 202 TTMAELEEVARAFVENDPDGNGQRDTIGITGPESGGKLYANFLE-SKNNLYGFDAIFSAH 260 Query: 254 HAFPTNWIRDSRGNVVYGSITPETKEALGKLAGWYKRGLIDPDFALYKETQEPIISGKAG 313 +FP W+ G+ VYGSI PET+EAL L Y +GLIDP+ + + EPII+GK+G Sbjct: 261 DSFPGYWLEGEDGSPVYGSILPETREALASLRDLYSKGLIDPEMGVRNKADEPIIAGKSG 320 Query: 314 MFFGPWWMPYYPLSEAVALDTRAEWRAYAAPLDASGKFVTHMAPVTDRYLVVRKGYEHPE 373 MFF PWWM Y PL+ AV D A W+AYA PL+ +G+F H A + ++VVRK YEHPE Sbjct: 321 MFFAPWWMAYGPLTNAVKNDAEANWQAYALPLNQNGEFRPHAATPSTEFVVVRKDYEHPE 380 Query: 374 AVVKLLNVFTRLERRRDPNLEAVRSLDDFSAQTGIQPRAYYPFDLLIDYADAIEKHYADM 433 +KLLN + E+ + D G YYP ++ +D IE + Sbjct: 381 VPMKLLNNLFKNEQE--------NTFDPSKGGPG-----YYPLRVVYAPSDEIEFTVKAL 427 Query: 434 QQALHG-------------KIDPD---SLDPDTHLLYDLWLADEEQPKKNLEGWKAANAY 477 + L G K+ P S+ + YD P+ N W + + Sbjct: 428 RDVLAGNKTAEDFANDKAYKLLPSDIASIKTIKNEPYDSMDLSTWNPEANWGAWTRSYSI 487 Query: 478 KYGVSVLSTTAIDRVRSVFYGSTLHMRSNWPELQKLESETFLNIIVGDSPLSDFDLFAAK 537 G L T + V S+ Y T M S W L+KLE ETFL II+G +PL FD F Sbjct: 488 MVGGRPLVDTEFEEVPSLLYEQTPLMESRWVNLRKLEDETFLRIIMGAAPLETFDSFVEN 547 Query: 538 WRSLGGDAITQEVAKAV 554 W+ GGD ITQEV A+ Sbjct: 548 WKRQGGDQITQEVKDAL 564 >A7VHE6.1 A7VHE6_9CLOT Putative uncharacterized protein Length = 568 Score = 319 bits (817), Expect = 6e-85, Method: Compositional matrix adjust. Identities = 188/521 (36%), Positives = 282/521 (54%), Gaps = 20/521 (3%) Query: 40 AESAAYGRYEEPVVMRIG--FKVPDSRLNTGDSNDNNPISRYLESLTNIKVIHSWEAKGE 97 A + YG+Y E + +G +S L G++ +NN +R L N++ +EA + Sbjct: 53 ARTTPYGKYPEQLTYTLGKLSGANNSNLPDGETYENNAYTRLLNERLNVQNQDVFEAM-D 111 Query: 98 EPFTQKVQLAIDSNDLPDAMVV-DRDQLEKLIDSDMIADLTQTYKEFGSELIKDMYDSTQ 156 E +T V + I NDLPD M+V D D+L+ L+D+DMIADLT +Y S+ IK++Y S Sbjct: 112 EQYTDSVTMVIAQNDLPDVMIVEDLDELQYLVDNDMIADLTDSYNNCMSDTIKNIYGSYG 171 Query: 157 GEALGDATRNGRLYGLPNVAIHADSPSLLWVRQDWLXXXXXPAPRSFEDIEAIAKAFIEQ 216 + L T G++ +P I +D P+L+W+R+DW+ APR+ D+E I + F E+ Sbjct: 172 RDILDVVTFGGKIMAIPETNI-SDGPNLIWLRKDWMDALGLSAPRTLSDVEEIIRQFKEK 230 Query: 217 DPDGNGKRDTVGLSAYNNIVYGTKPHVN-GLDTLFSALHAFPTNWIRDSRGNVVYGSITP 275 DP NG +TVGL ++ G LD +F+A AFP WI D GNVVYGS+ P Sbjct: 231 DPGHNGAGNTVGLVCDTSLCGGCGYSSEYTLDIIFAAYGAFPKQWIYDEDGNVVYGSVQP 290 Query: 276 ETKEALGKLAGWYKRGLIDPDFALYKETQ--EPIISGKAGMFFGPWWMPYYPLSEAVALD 333 E KEAL + YK G++D DF + + E I+ G+ G FFGPWW P PL +AV + Sbjct: 291 EAKEALAHIHELYKEGILDQDFLMRTSSNLIELIVDGQCGSFFGPWWAPNNPLMQAVEQN 350 Query: 334 TRAEWRAYAAPLDASGKFVTHMAPVTDRYLVVRKGYEHPEAVVKLLNVFTRLERRRD-PN 392 AEW+ Y + SG H + +Y+VVRKGYE+PE K+++V R D N Sbjct: 351 KDAEWQPYLIATEKSGLTSYHTQNPSGKYIVVRKGYEYPEIACKIVSVLFDYLRYNDRDN 410 Query: 393 LEAVRSLDDFSAQTGIQPRAYYPFDLLIDYADAIEKHYADMQQALHGKIDPDSLDPDTHL 452 E V D+ + + P A PF + +DY +A++ Y ++ G D L+ + Sbjct: 411 QEIV----DYYKEN-VDPTA-RPFAINVDYNNALQICYGELNHVFAGDKSADDLNVLEYS 464 Query: 453 LYDL---WLADEEQPKKNLEGWKAANAYKYGVSVLSTTAIDRVRSVFYGSTLHMRSNWPE 509 Y+ +L D E E W A + +L+ ++V S+++G T M ++W Sbjct: 465 YYEACESYLKDAENASA--EDWAAYTSRITACKILNDGRTNKVESLYFGETETMVTDWWS 522 Query: 510 LQKLESETFLNIIVGDSPLSDFDLFAAKWRSLGGDAITQEV 550 L+ LES+T+L I+ G+S L +FD F W GG+ IT+EV Sbjct: 523 LENLESDTYLKIVTGESSLDEFDRFVENWYQNGGETITKEV 563 >A8SQM2.1 A8SQM2_9FIRM Putative uncharacterized protein Length = 569 Score = 306 bits (785), Expect = 3e-81, Method: Compositional matrix adjust. Identities = 177/524 (33%), Positives = 279/524 (53%), Gaps = 18/524 (3%) Query: 40 AESAAYGRYEEPVVMRIG--FKVPDSRLNTGDSNDNNPISRYLESLTNIKVIHSWEAKGE 97 AE+ GRY E + +G +S L G++ +NN ++ L + N++ +EA + Sbjct: 54 AETTPLGRYPEQLTYTLGKLSGANNSNLPQGETYENNAYTQILNEILNVQNQDIFEA-AD 112 Query: 98 EPFTQKVQLAIDSNDLPDAMVV-DRDQLEKLIDSDMIADLTQTYKEFGSELIKDMYDSTQ 156 + V + I DLPD M+V D D+L+ L+++DMI DLT +Y SE IK++Y S Sbjct: 113 NQYDDNVSMVIAQKDLPDVMIVQDMDELQYLVENDMIEDLTDSYNNCMSETIKNIYKSYG 172 Query: 157 GEALGDATRNGRLYGLPNVAIHADSPSLLWVRQDWLXXXXXPAPRSFEDIEAIAKAFIEQ 216 + T + R+ +P I +D P+L+W+R+DW+ APR+ D E I + FIE+ Sbjct: 173 SSIMDVVTFDDRIMAIPETNI-SDGPNLIWLRKDWMDKLGLDAPRTLSDAEEIIRQFIEK 231 Query: 217 DPDGNGKRDTVGLSAYNNIVYGTKPHVN-GLDTLFSALHAFPTNWIRDSRGNVVYGSITP 275 DP NG+ +TVGL A ++ G LD +F+A AFP WI+D G VVYGS+ P Sbjct: 232 DPGNNGEGNTVGLVADTSLCGGCGYSSEYTLDIVFAAYGAFPKQWIKDEDGKVVYGSVQP 291 Query: 276 ETKEALGKLAGWYKRGLIDPDFALYKETQ--EPIISGKAGMFFGPWWMPYYPLSEAVALD 333 E K+AL + Y+ ++D +F + + E I++G+ G FFGPWW P PL +AVA D Sbjct: 292 EAKKALEHIHEMYEEEILDQNFLMRTSSNIIELIVNGQCGSFFGPWWAPNNPLMDAVADD 351 Query: 334 TRAEWRAYAAPLDASGKFVTHMAPVTDRYLVVRKGYEHPEAVVKLLNVFTRLERRRDPNL 393 A+W Y + +G H ++VV+KGYEHPE V K+++V R D + Sbjct: 352 PTAKWEPYLLATEENGSTSYHTQVPYGNFVVVKKGYEHPEIVCKIISVLFDYVRYEDKDN 411 Query: 394 EAVRSLDDFSAQTGIQPRAYYPFDLLIDYADAIEKHYADMQQALHGKIDPDSLDPDTHLL 453 +A++ + + P A P + +DY +A++ Y ++ G PD L+ Sbjct: 412 QAIKDY----YKLNVDPTA-RPLAMNVDYNNALKICYGELSHVFSGVRQPDDLNLLEQSY 466 Query: 454 Y---DLWLADEEQPKKNLEGWKAANAYKYGVSVLSTTAIDRVRSVFYGSTLHMRSNWPEL 510 Y D +L +E+ E W A + +L+ ++V S+++G T M S+W L Sbjct: 467 YEACDSYLKNEDNASS--EDWAAYTSRITACKILNDARTNKVESLYFGETETMMSDWWHL 524 Query: 511 QKLESETFLNIIVGDSPLSDFDLFAAKWRSLGGDAITQEVAKAV 554 + LES+T+L I+ G++ L +FD F W GG IT+EV + Sbjct: 525 ENLESDTYLKIVTGEADLDEFDSFVDNWYKTGGTTITKEVRREC 568 >C4Z3A7.1 C4Z3A7_9FIRM Multiple sugar transport system substrate-binding protein Length = 566 Score = 288 bits (738), Expect = 9e-76, Method: Compositional matrix adjust. Identities = 176/532 (33%), Positives = 285/532 (53%), Gaps = 22/532 (4%) Query: 36 RAIKAESAAYGRYEEPVVMRIG--FKVPDSRLNTGDSNDNNPISRYLESLTNIKVIHSWE 93 R +A + +G Y E + +G V +S + D+ +N +RY++S+ N++ + +E Sbjct: 43 RMEEARTTPFGAYPETIEYTLGKMTSVNNSNMPENDTYTDNAYTRYIKSVINVQNVDVFE 102 Query: 94 AKGEEPFTQKVQLAIDSNDLPDAMVVD-RDQLEKLIDSDMIADLTQTYKEFGSELIKDMY 152 A + + V + I LPD MVV +D++E+L+++ +I DLT++Y S+ I+ MY Sbjct: 103 ANDSQ-YDTNVSMVISMGSLPDIMVVSSQDEVEQLVEAGLIEDLTESYNNCISDRIRKMY 161 Query: 153 DSTQGEALGD-ATRNGRLYGLPNVAIHADSPSLLWVRQDWLXXXXXPAPRSFEDIEAIAK 211 +S G++L D T +G++ LP I D P+L+W+R+DW+ P + +D+ I K Sbjct: 162 ES-YGDSLKDMVTYDGKIMALPETNI-TDGPNLVWLRKDWMDKLGLSEPHTIDDVVNIVK 219 Query: 212 AFIEQDP-----DGNGKRDTVGLSAYNNIVYGTKPHVNG--LDTLFSALHAFPTNWIRDS 264 FI +DP D +GK +TVGL A + V G + + LD +F+ A+P WI + Sbjct: 220 HFISEDPGNNGVDASGKPNTVGL-AVDTDVTGECGYSSEFLLDIIFACFGAYPKQWIMND 278 Query: 265 RGNVVYGSITPETKEALGKLAGWYKRGLIDPDFALYKETQ--EPIISGKAGMFFGPWWMP 322 G +VYGS+T E KEAL ++ Y +G+ID DF L T E I +G G FFGPWW P Sbjct: 279 DGEIVYGSVTNEAKEALSYISSLYNQGVIDNDFLLRTSTNICELIENGLCGSFFGPWWAP 338 Query: 323 YYPLSEAVALDTRAEWRAYAAPLDASGKFVTHMAPVTDRYLVVRKGYEHPEAVVKLLNVF 382 PL+ AV+ + A+W+ Y D+ G H +Y+VVRKGYEHPE K+++V Sbjct: 339 NNPLANAVSRNPDADWQPYLIATDSDGTTSYHSQNPCYKYVVVRKGYEHPEIAAKMISVM 398 Query: 383 TRLERRRDPNLEAVRSLDDFSAQTGIQPRAYYPFDLLIDYADAIEKHYADMQQALHGKID 442 R + E ++ Q ++P A P + +DY +A+ Y ++ + G+ + Sbjct: 399 FDKVRFDCTDSEEFKNY----YQLNVEPTA-RPLSINVDYNNALSICYRNIDATISGRKN 453 Query: 443 PDSLDPDTHLLYDLWLADEEQPKKNLEGWKAANAYKYGVSVLSTTAIDRVRSVFYGSTLH 502 PDSL+ YD + K W A + S+++ I V S+++ +T Sbjct: 454 PDSLELLERSFYDACSEYIKNANKTSTQWAAYMSRIKACSLIAQDNIKVVDSLYFKTTDT 513 Query: 503 MRSNWPELQKLESETFLNIIVGDSPLSDFDLFAAKWRSLGGDAITQEVAKAV 554 M+S+W L+ E E +L II G+ +S FD F +W GG IT EV++++ Sbjct: 514 MKSHWWRLKAKEKEAYLKIISGEEDISYFDRFVKEWNEQGGQTITSEVSESM 565 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.135 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,493,834,093 Number of extensions: 151057145 Number of successful extensions: 325663 Number of sequences better than 1.0e-05: 157 Number of HSP's gapped: 325458 Number of HSP's successfully gapped: 157 Length of query: 565 Length of database: 3,060,696,203 Length adjustment: 141 Effective length of query: 424 Effective length of database: 1,732,333,088 Effective search space: 734509229312 Effective search space used: 734509229312 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 134 (56.2 bits)