BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0260.0027.p (583 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KEX4.1 B3KEX4_9BACI Histidine kinase internal region 348 1e-93 B1D9Q3.1 B1D9Q3_9BACL Integral membrane sensor signal transducti... 320 2e-85 B1DFU1.1 B1DFU1_9BACL Integral membrane sensor signal transducti... 292 7e-77 B1D916.1 B1D916_9BACL Integral membrane sensor signal transducti... 236 4e-60 C4ZHY0.1 C4ZHY0_9FIRM Two-component sensor histidine kinase 174 2e-41 >B3KEX4.1 B3KEX4_9BACI Histidine kinase internal region Length = 623 Score = 348 bits (893), Expect = 1e-93, Method: Compositional matrix adjust. Identities = 192/514 (37%), Positives = 293/514 (57%), Gaps = 13/514 (2%) Query: 61 LQQEALVLENAIDNLDFASKQFALDGQIVEEMSSFLEEKQIYRKSQIMNSINQKINLVNF 120 L+Q L LEN +DNL+ S+Q G + ++ FL KQ Y +S + + I ++NL+ F Sbjct: 101 LKQVRLSLENTLDNLNHVSQQLTFKGNVGRQLEKFLMAKQPYDRSYLTDEIKNQLNLITF 160 Query: 121 TNPYIGLTAYIMPGSDDPVLFTNMSTRRDFDPGRLPAFMRYNGADYYGPHGTMYAVGDNV 180 TNP +GLT Y + D LF N ++ FD LP Y G Y+GPH + + Sbjct: 161 TNPNVGLTLYYLR-DDQTTLFENTGMKQPFDIDGLPIIAAYYGITYFGPHISKDRFNNQY 219 Query: 181 VFSERRIVRVNGQHELYIYLETNYSLLRKIFNPQAYGMQVNHLLVNQNNTDIHVIDGELP 240 V S R V + + + Y+Y+E+ + L + I + NHL+++ N + EL Sbjct: 220 VLSALRKVDLPQREDAYVYIESGFKLTQSILDLDEVSKHANHLILDNNG---RISYSELQ 276 Query: 241 ASVVEAARASTGPAHEVLDG----YHLFRYESLQGWKLVAAVKKSVFNSEISSWFYKMIX 296 E + S A ++ G Y+ +R S QGW +V+ + KS +N E + W +M+ Sbjct: 277 DIFPENSIFSDALAPSLVTGITSDYYWYRQVSNQGWSVVSLIAKSDYNREKNRWMMQMLG 336 Query: 297 XXXXXXXXXXXXXXXIWKRIYGPLRKVNLEIIRMA-ENVTAPVAFTNVAEFDFVLMNFQQ 355 +WK +Y PL + N EI +A N A + + EF+ VL FQ Sbjct: 337 LALLFAVVSLTIGWLLWKMVYRPLSRFNKEIKSIAGSNFNAAATQSRIPEFEMVLNQFQH 396 Query: 356 MKDQVNELILAVARNEKQKSQFEIEKLLSQINPHFLHNTLNTVQWLARLNGQKEIDKLVT 415 MK Q+ +L V EK+++ EIEKL QINPHFL NTL+T WLA +NGQ+EID+LVT Sbjct: 397 MKVQITQLYSEVKAKEKRRADLEIEKLQYQINPHFLMNTLDTAHWLAVMNGQEEIDRLVT 456 Query: 416 LLVKVLHYNLGKQSIIVTIGEEIEAIRNYMELQRIRYDYEFEFNVQADETVLDAAVPRFL 475 L K+L+YNL K + + TI +EI++++ Y+ LQ+IRY+++F+ N+ D+ +L+ VPRF+ Sbjct: 457 SLNKLLYYNLRKGNSVATIRDEIDSLKQYLILQQIRYNFQFDVNIHVDDALLEVTVPRFI 516 Query: 476 LQPLVENSIYHGLSDNGRVDVIITAHGPGEVQLCVRDSGTGMDEAQIAELLADDGVAGR- 534 LQPLVENS+YHGL DNGR++V++ + E++ +RD+G G+ E +I LL + Sbjct: 517 LQPLVENSLYHGLDDNGRIEVVVRKNDRLEIE--IRDNGGGISEEKIEALLEQEQTERHR 574 Query: 535 -GLGIGFSYVMRMLRTYYGEQMTLQIHSAANEGT 567 G+GIG +YV RML +Y + +L + S +GT Sbjct: 575 VGMGIGLNYVKRMLEFHYEGKASLTVTSELGKGT 608 >B1D9Q3.1 B1D9Q3_9BACL Integral membrane sensor signal transduction histidine kinase Length = 580 Score = 320 bits (821), Expect = 2e-85, Method: Compositional matrix adjust. Identities = 193/530 (36%), Positives = 292/530 (55%), Gaps = 24/530 (4%) Query: 61 LQQEALVLENAIDNLDFASKQFALDGQIVEEMSSFLEEKQIYRKSQIMNSINQKINLVNF 120 LQ+ + LE ++NL S+Q + G + +M + + Y KSQ++ +I + + ++N Sbjct: 61 LQRVSRSLEETLENLYDVSQQMSYQGFVGRDMEALQTSEDNYEKSQLVVNIQRNMGILNA 120 Query: 121 TNPYIGLTAYIMPGSDDPVLFTNMSTRR---DFDPGRLPAFMRYNGADYYGPHGTMYAVG 177 +NP IGL Y + + + + N TR DFD LP ++ + GPH T+Y Sbjct: 121 SNPTIGLVFYYDTTTGE-IQYNNYITRDLHPDFD--SLPKLSVHSDHVFNGPHLTLYKYN 177 Query: 178 DNVVFSERRIVRVNGQ-----HELYIYLETNYSLLRKIFNPQAYGMQVNHLLVNQNNTDI 232 D VFS IVR G + L +Y ETN S+++ I N + YGM+V+HLL++++ T Sbjct: 178 DIPVFS---IVRSVGGITDPGNPLLVYAETNVSVIKNILNKEQYGMKVSHLLMDKDGT-- 232 Query: 233 HVIDGELPASVVEAARASTGPAHEVLDGYHLFRYESLQGWKLVAAVKKSVFNSEISSWFY 292 V+ + P + G L Y+ QGW+L V+K + EI WF Sbjct: 233 -VLFSDKPELTPIGSTYDPGK----LKRYYFHEVTGSQGWRLGVFVEKGEYQKEIRDWFV 287 Query: 293 KMIXXXXXXXXXXXXXXXXIWKRIYGPLRKVNLEIIR-MAENVTAPVAFTNVAEFDFVLM 351 K WK + PL ++ I +++ A T + EFDF++ Sbjct: 288 KYAFIVVLGLLISIWSAFRTWKVVISPLHRIRKGISAVISQEPNANFQMTGLREFDFLIQ 347 Query: 352 NFQQMKDQVNELILAVARNEKQKSQFEIEKLLSQINPHFLHNTLNTVQWLARLNGQKEID 411 NF M+ ++ +LI+ VA+ +K + E+EKL++QINPHFL+NTLNT QWLARL GQ EID Sbjct: 348 NFNDMQFRIQKLIMEVAKEASEKQEIEMEKLMAQINPHFLYNTLNTAQWLARLKGQHEID 407 Query: 412 KLVTLLVKVLHYNLGKQSIIVTIGEEIEAIRNYMELQRIRYDYEFEFNVQADETVLDAAV 471 + ++L KVL YNL K IVT+ +EIE++ +Y+ELQ+IRYD+EF+ E L + Sbjct: 408 RFLSLFTKVLKYNLAKDGAIVTLKDEIESLNDYVELQKIRYDFEFDVRYSIPEPFLATPL 467 Query: 472 PRFLLQPLVENSIYHGLSDNGRVDVIITAHGPGEVQLCVRDSGTGMDEAQIAELLADDGV 531 PRF+LQP+VEN++YHG+ D + + G + L V+D+G G+ +I ELL D Sbjct: 468 PRFILQPIVENALYHGMMDEKGIIEVTAEEREGMLVLMVKDNGNGISPEKIKELLESDTR 527 Query: 532 AGR--GLGIGFSYVMRMLRTYYGEQMTLQIHSAANEGTTVSIIIPIKGKE 579 GLGIG +YV+R L ++ G L+I S EGTTVSI +P+ +E Sbjct: 528 KNNISGLGIGLNYVVRTLSSFTGGASQLRIDSQIGEGTTVSIHMPMHEEE 577 >B1DFU1.1 B1DFU1_9BACL Integral membrane sensor signal transduction histidine kinase Length = 582 Score = 292 bits (747), Expect = 7e-77, Method: Compositional matrix adjust. Identities = 174/522 (33%), Positives = 283/522 (54%), Gaps = 16/522 (3%) Query: 68 LENAIDNLDFASKQFALDGQIVEEMSSFLEEKQIYR-KSQIMNSINQKINLVNFTNPYIG 126 +++++DNL +AS+Q LDG I + + ++ KS+I + I ++ L+++ N +G Sbjct: 64 MQSSLDNLGYASRQLTLDGDIGKRLEQYMNPTIPNNLKSEIQSDIESRLTLISYANQGLG 123 Query: 127 LTAYIMPGSDDPVLFTNMSTRRDFDPGRLPAFMRYNGADYYGPHGTMYAVGDNVVFSERR 186 Y +P LF M +++ DP P Y GPH ++Y DN+VFS Sbjct: 124 PMYYFIP-EQQMNLFQTMLLKKNIDPMEYPTLFTDPLTLYQGPHPSIYLYSDNIVFS--I 180 Query: 187 IVRVNGQHEL---YIYLETNYSLLRKIFNPQAYGMQVNHLLVNQNNTDIHVIDGELPASV 243 + + G L Y+Y+ETN+ + + +F A+G VN+L+++ +NT V+ +L S Sbjct: 181 VQEMTGNKNLPKTYVYIETNFKVFQDLFPKDAFGFNVNYLILDSSNT---VLYSDLTDSY 237 Query: 244 -VEAARASTGPAHEVLD--GYHLFRYESLQGWKLVAAVKKSVFNSEISSWFYKMIXXXXX 300 + + + +G +V++ G LF W +VA V K + EI SW + Sbjct: 238 PINSEMSLSGSQSQVINDKGKKLFVQRGEGNWSIVAVVNKRDLSDEIISWSIRYGAIAVG 297 Query: 301 XXXXXXXXXXXIWKRIYGPLRKVNLEIIRMAE-NVTAPVAFTNVAEFDFVLMNFQQMKDQ 359 W+ I L + EI + + P N+ EFD V+ F M+ + Sbjct: 298 SLIISLLLAWMTWRFINQKLSLLKEEIKQTTDLQFDRPYVPMNIIEFDEVVDRFYLMRTR 357 Query: 360 VNELILAVARNEKQKSQFEIEKLLSQINPHFLHNTLNTVQWLARLNGQKEIDKLVTLLVK 419 + +LI+ V EK K + E++KL QINPHF+HNTLNT+QW+AR++ Q+EI LV++ + Sbjct: 358 IRDLIVEVEEKEKNKRKLEVQKLKYQINPHFIHNTLNTIQWIARMHKQEEIVNLVSIFSR 417 Query: 420 VLHYNLGKQSIIVTIGEEIEAIRNYMELQRIRYDYEFEFNVQADETVLDAAVPRFLLQPL 479 +LHYNLGK+ IV + EEI A+++Y+ LQ+IRY++ F + + + + RFL+QPL Sbjct: 418 ILHYNLGKEGEIVHLREEISALKDYVALQKIRYNHHFHVLFEIEPDTEELPIIRFLIQPL 477 Query: 480 VENSIYHGLS-DNGRVDVIITAHGPGEVQLCVRDSGTGMDEAQIAELL-ADDGVAGRGLG 537 VEN++YH + DNG++ V + + V D+G GMD I LL +D+ G+G Sbjct: 478 VENAMYHAFTDDNGQITVRVFKENEAYFIIEVEDNGQGMDTETIQRLLYSDNEKRKSGMG 537 Query: 538 IGFSYVMRMLRTYYGEQMTLQIHSAANEGTTVSIIIPIKGKE 579 IG +V M+R Y+GE+ +I S +GT + I +PI KE Sbjct: 538 IGLKFVNHMIREYFGEEHRFEISSELGKGTMIRIRLPILDKE 579 >B1D916.1 B1D916_9BACL Integral membrane sensor signal transduction histidine kinase Length = 592 Score = 236 bits (603), Expect = 4e-60, Method: Compositional matrix adjust. Identities = 156/514 (30%), Positives = 258/514 (50%), Gaps = 25/514 (4%) Query: 73 DNLDFASKQFALDGQIVEEMSSFLEEKQIYRKSQIMNSINQKINLVNFTNPYIGLTAYIM 132 +NL ++Q +G + M +++ IY + + I+ I L+ F+NP + L Y Sbjct: 82 NNLLQVTQQMTPEGTVGHLMETYVSTADIYSQRLLSRDISYNIGLITFSNPAMQLVMYYN 141 Query: 133 PGSDDPVLFTNMSTRRDFDPGRLPAFMRYNGADYYGPHGTMYAVGDNVVFSERRIVRVNG 192 + L TN+ R DF LP Y PH ++ D++V S R V + Sbjct: 142 EANGQAAL-TNLPVRADFKLESLPDVAGMGELRYQSPHKSLCRFSDDLVVSVTREVTFSD 200 Query: 193 QHELYIYLETNYSLLRKIFNPQAYGMQVNHLLVNQNNTDIHVIDGELPASVVEAA----- 247 +L +Y+E + + + +++ + LV + +G + S EAA Sbjct: 201 GTKLVVYVEAKTDIPTDM-SSVTESLKMPYSLVFTDP------EGYVTYSSNEAAFKAGQ 253 Query: 248 ----RASTGPAHEVLDGYHLFRYESLQGWKLVAAVKKSVFNSEISSWFYKMIXXXXXXXX 303 + +G D Y R G+ + + S +N E+ +W M Sbjct: 254 HLELKGQSGTT----DEYVWNRMTGDYGYDITMLLPVSYYNHELYTWKNHMYWILAIALL 309 Query: 304 XXXXXXXXIWKRIYGPLRKVNLEIIRMAENVTAPVAF-TNVAEFDFVLMNFQQMKDQVNE 362 + + I P R + E+ + + T + EFD + F MK Q+ + Sbjct: 310 MMFMLTLVLRRLINRPFRLIEKEMRTFGKGYMGTTQYHTGIDEFDRIFDQFNIMKRQIQK 369 Query: 363 LILAVARNEKQKSQFEIEKLLSQINPHFLHNTLNTVQWLARLNGQKEIDKLVTLLVKVLH 422 LI+ V + EK++ Q EIEKL QINPHFL NTLN+V+WLA ++ Q+EI+K ++ L+ +L+ Sbjct: 370 LIIDVEQKEKRRHQLEIEKLAYQINPHFLMNTLNSVRWLAVMHKQEEIEKFISSLIILLN 429 Query: 423 YNLGKQSIIVTIGEEIEAIRNYMELQRIRYDYEFEFNVQADETVLDAAVPRFLLQPLVEN 482 YNLGK T EI+ +R Y+ELQ++R+D+E + ++ A+ T LD+ V RF+LQP+VEN Sbjct: 430 YNLGKSEEKATFRSEIQVMRTYLELQQMRHDFEVKLDI-AEGTYLDSPVARFILQPIVEN 488 Query: 483 SIYHGLSDNGRVDV-IITAHGPGEVQLCVRDSGTGMDEAQIAELLADDGVAGR-GLGIGF 540 ++ HGL DNG +++ ++ V++ +RD G G+ + +A L D + G GIG Sbjct: 489 AVCHGLDDNGMLEISVVPDEAAQMVRITIRDDGKGLSQETLALLQRDTPDQQQTGWGIGL 548 Query: 541 SYVMRMLRTYYGEQMTLQIHSAANEGTTVSIIIP 574 YV ML + YG + + I S N+GTTV++ +P Sbjct: 549 RYVRSMLESSYGHKARMGIDSTPNQGTTVTLTLP 582 >C4ZHY0.1 C4ZHY0_9FIRM Two-component sensor histidine kinase Length = 383 Score = 174 bits (442), Expect = 2e-41, Method: Compositional matrix adjust. Identities = 103/325 (31%), Positives = 180/325 (55%), Gaps = 8/325 (2%) Query: 259 DGYHLFRYESLQGWKLVAAVKKSVFNSEISSWFYKMIXXXXXXXXXXXXXXXXIWKRIYG 318 +GY + Y S G+ A+ ++++ E++ W K++ +++ I Sbjct: 39 EGYKIMVYRSTIGYANAIALPENIYQKEMNMWRLKLVVIILSAFGIFSCSVIYLYRLICK 98 Query: 319 PLRKVNLEIIRMAEN-VTAPVAFTNVAEFDFVLMNFQQMKDQVNELILAVARNEKQKSQF 377 PL ++ +II++ + + EFD ++ +QMK+Q+ LI V EK + Sbjct: 99 PLNQLQKQIIQVGNGALHGERQEYEIKEFDDLMHEVEQMKEQIRNLIDDVVEKEKNIQRT 158 Query: 378 EIEKLLSQINPHFLHNTLNTVQWLARLNGQKEIDKLVTLLVKVLHYNLGKQSIIVTIGEE 437 E EKLL QINPHFL NTLN+VQW+AR++ Q I + V L ++L YNLGK+ + T+ E Sbjct: 159 EYEKLLYQINPHFLLNTLNSVQWMARMSKQNNIMEFVQRLKRLLSYNLGKEGMQTTLRTE 218 Query: 438 IEAIRNYMELQRIRYDYEFEFNVQADETVLDAAVPRFLLQPLVENSIYHGLSDNGRVDV- 496 I+ +++Y+ L+++RYD+ E NV+ + L+ R LLQPLVEN+I +GL ++ ++ + Sbjct: 219 IDIVKDYIALEQMRYDFVIEMNVE-EGRYLEQPTVRMLLQPLVENAIRYGLGNDEKITIQ 277 Query: 497 IITAHGPGEVQLCVRDSGTGMDEAQIAELLADDGVAGRGL-----GIGFSYVMRMLRTYY 551 + + G + + DSG G+ + +I ++ + + GIG YV ML ++Y Sbjct: 278 VFEDNIRGLAGITILDSGNGLTQEEIYQINEPFDYDVKKMQHGNRGIGLRYVKAMLDSFY 337 Query: 552 GEQMTLQIHSAANEGTTVSIIIPIK 576 + TL ++ GT ++I+IPI+ Sbjct: 338 EGETTLFVNCKKGYGTKITILIPIQ 362 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.137 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,082,487,403 Number of extensions: 127177286 Number of successful extensions: 317797 Number of sequences better than 1.0e-05: 1994 Number of HSP's gapped: 315084 Number of HSP's successfully gapped: 1994 Length of query: 583 Length of database: 3,060,696,203 Length adjustment: 141 Effective length of query: 442 Effective length of database: 1,732,333,088 Effective search space: 765691224896 Effective search space used: 765691224896 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)