BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0260.0025.p (300 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B8I621.1 B8I621_CLOCE Binding-protein-dependent transport system... 357 5e-97 B1S932.1 B1S932_9BIFI Putative uncharacterized protein 353 1e-95 C2AXC9.1 C2AXC9_9MICO Carbohydrate ABC transporter membrane prot... 337 1e-90 A9KQK9.1 A9KQK9_CLOPH Binding-protein-dependent transport system... 283 1e-74 A4XJ75.1 A4XJ75_CALS8 Binding-protein-dependent transport system... 196 1e-48 >B8I621.1 B8I621_CLOCE Binding-protein-dependent transport systems inner membrane component Length = 307 Score = 357 bits (917), Expect = 5e-97, Method: Compositional matrix adjust. Identities = 175/304 (57%), Positives = 227/304 (74%), Gaps = 4/304 (1%) Query: 1 VIHLRKKLKPGDALFQGIIYVLFGLFTLSCIYPFYYLFINTISSNDLSAAGDINFYPQQI 60 ++ +++ GD LF IIYV+F + T++CIYPFY+L INT+++ND+S+ + PQ+I Sbjct: 4 ILEKKQRYSLGDILFFIIIYVVFTVLTIACIYPFYFLIINTLTANDISSKAAVLLLPQKI 63 Query: 61 HFDNYSKVLRISGLSHAALVSVYRTVAGTFLTVGASAFLGYLFTKKEMWGRQIWYRSVVV 120 HFDNY K+L ++GL+ A +VSV RT+ G LTV ASAFLGYLFTKKEMWGR+ WYR V+V Sbjct: 64 HFDNYLKILNLNGLTDALIVSVARTIIGAALTVFASAFLGYLFTKKEMWGRKFWYRFVIV 123 Query: 121 TMYFSAGLIPWYITMHNLHLTNNYLAYILPTIITPFNIILVKTFVEAIPPSLEESAAMDG 180 TMYF+AGLIPWYITM NL L NN+LAYILP I+ PFNIILVKT++E P +L+E+A +DG Sbjct: 124 TMYFNAGLIPWYITMLNLGLRNNFLAYILPAIVAPFNIILVKTYIENTPQALQEAAQIDG 183 Query: 181 AGVLRSFWSIVVXXXXXXXXXXXXFSAVNQWNSFIDTAFLMTDTKYYTLQFLLWRYLNES 240 AG F +V F+AV QWNSF DT LMTD+ YTLQF+L+RYLN++ Sbjct: 184 AGYFTIFIKVVWPLCVPILATVTIFAAVGQWNSFTDTLLLMTDSHLYTLQFILYRYLNQA 243 Query: 241 SSLAALIRSS----PDMAASVQNMQTPTSVRMTVTMIVVLPILIVYPFFQRFFVKGIMIG 296 SSLA+++R+S +S+ QT SVRMTV+++V+LPIL VYPFFQRFFV GI+IG Sbjct: 244 SSLASIMRNSGAGIGQQVSSLAGSQTAQSVRMTVSVVVILPILFVYPFFQRFFVGGIIIG 303 Query: 297 AVKG 300 AVKG Sbjct: 304 AVKG 307 >B1S932.1 B1S932_9BIFI Putative uncharacterized protein Length = 310 Score = 353 bits (906), Expect = 1e-95, Method: Compositional matrix adjust. Identities = 171/293 (58%), Positives = 224/293 (76%), Gaps = 3/293 (1%) Query: 11 GDALFQGIIYVLFGLFTLSCIYPFYYLFINTISSNDLSAAGDINFYPQQIHFDNYSKVLR 70 G+ ++ + F +F C+YPFYYL IN+ISSN+ SAAG +N++PQ IH++NY +V Sbjct: 18 GEEVYNVFNILFFLVFAFICVYPFYYLIINSISSNNASAAGQVNWWPQGIHWENYKQVFA 77 Query: 71 ISGLSHAALVSVYRTVAGTFLTVGASAFLGYLFTKKEMWGRQIWYRSVVVTMYFSAGLIP 130 +SGL+ +A +SV RTV GT LTV ASAFLG++FT+++MW R+ WYR +V+TMYF+AGLIP Sbjct: 78 LSGLTTSAFISVARTVIGTVLTVIASAFLGFMFTQEKMWHRKFWYRFIVITMYFNAGLIP 137 Query: 131 WYITMHNLHLTNNYLAYILPTIITPFNIILVKTFVEAIPPSLEESAAMDGAGVLRSFWSI 190 +ITM NLHLTNN+ YILP I+ PFNIILVKTFVE+IP SL+E+A MDGAG L FW I Sbjct: 138 MFITMKNLHLTNNFWVYILPAIVQPFNIILVKTFVESIPASLQEAAEMDGAGTLTIFWKI 197 Query: 191 VVXXXXXXXXXXXXFSAVNQWNSFIDTAFLMTDTKYYTLQFLLWRYLNESSSLAALIRSS 250 V+ FSAV QWNSF DT MTD+K Y+LQ++L+ Y+N++++LAA +R+S Sbjct: 198 VLPMMAPILATVGIFSAVGQWNSFQDTLIYMTDSKLYSLQYVLYTYINQANALAASVRAS 257 Query: 251 PDMA---ASVQNMQTPTSVRMTVTMIVVLPILIVYPFFQRFFVKGIMIGAVKG 300 A A++ QTPTS+RMTV++IVVLPIL VYPFFQRFFVKG+M+GAVKG Sbjct: 258 AGSAMNVAALATQQTPTSIRMTVSVIVVLPILFVYPFFQRFFVKGMMLGAVKG 310 >C2AXC9.1 C2AXC9_9MICO Carbohydrate ABC transporter membrane protein 2, CUT1 family Length = 305 Score = 337 bits (863), Expect = 1e-90, Method: Compositional matrix adjust. Identities = 164/295 (55%), Positives = 220/295 (74%), Gaps = 3/295 (1%) Query: 9 KPGDALFQGIIYVLFGLFTLSCIYPFYYLFINTISSNDLSAAGDINFYPQQIHFDNYSKV 68 + GD +F G Y+ F LF L C YPF+YL +N++S+NDLSAAGDI +P+ +H NYS++ Sbjct: 11 EKGDRVFAGWNYLWFALFALLCAYPFWYLVVNSVSANDLSAAGDIRLWPRGLHLSNYSQI 70 Query: 69 LRISGLSHAALVSVYRTVAGTFLTVGASAFLGYLFTKKEMWGRQIWYRSVVVTMYFSAGL 128 L+++GLS AA+VSV RTV GT TV ASAFLG++FT++ MW R +WYR VV+TMYF+AGL Sbjct: 71 LQLNGLSTAAIVSVARTVIGTAATVLASAFLGFMFTQERMWKRSLWYRLVVITMYFNAGL 130 Query: 129 IPWYITMHNLHLTNNYLAYILPTIITPFNIILVKTFVEAIPPSLEESAAMDGAGVLRSFW 188 IP Y+TM L LTN++ YILP I+ PFN+ILVKT++E++P SL+E+A +DGA LR F+ Sbjct: 131 IPVYVTMKTLGLTNSFWVYILPAIVQPFNVILVKTYIESLPRSLQEAAEVDGASTLRIFF 190 Query: 189 SIVVXXXXXXXXXXXXFSAVNQWNSFIDTAFLMTDTKYYTLQFLLWRYLNESSSLAALIR 248 SI++ FSAV QWNSF DT +TD K Y+LQ+LL+ ++N++SSLA + Sbjct: 191 SIILPNTTPILATVAIFSAVAQWNSFQDTLLYITDQKLYSLQYLLYMFINQASSLAQAAQ 250 Query: 249 SSP---DMAASVQNMQTPTSVRMTVTMIVVLPILIVYPFFQRFFVKGIMIGAVKG 300 ++ + AS QTPTS+RMTV +IVVLPI+ VYP FQR+FVKGIM+GAVKG Sbjct: 251 NAQGNLGIIASAATSQTPTSIRMTVAVIVVLPIVFVYPLFQRYFVKGIMLGAVKG 305 >A9KQK9.1 A9KQK9_CLOPH Binding-protein-dependent transport systems inner membrane component Length = 330 Score = 283 bits (725), Expect = 1e-74, Method: Compositional matrix adjust. Identities = 141/299 (47%), Positives = 197/299 (65%), Gaps = 3/299 (1%) Query: 5 RKKLKPGDALFQGIIYVLFGLFTLSCIYPFYYLFINTISSNDLSAAGDINFYPQQIHFDN 64 R K+ D +F+ I + F +FT +CI+PFYY+FINT+S NDL G +NF PQ H N Sbjct: 32 RFKMTVKDLIFEIINHSFFLIFTFACIFPFYYIFINTVSDNDLVRKGAVNFIPQGFHIKN 91 Query: 65 YSKVLRISGLSHAALVSVYRTVAGTFLTVGASAFLGYLFTKKEMWGRQIWYRSVVVTMYF 124 Y + ++ L + V++ RT+ GT L V AS F+GYL TK EMWGR+IWYR +VVTMYF Sbjct: 92 YLALANVNDLITSLTVTLSRTIIGTILMVVASGFVGYLVTKNEMWGRKIWYRFIVVTMYF 151 Query: 125 SAGLIPWYITMHNLHLTNNYLAYILPTIITPFNIILVKTFVEAIPPSLEESAAMDGAGVL 184 +AGLIPWYI M L LT+N+LAYI+P I+ P+NIILVKT++E+IP LEESA +DGA + Sbjct: 152 NAGLIPWYINMSMLGLTDNFLAYIIPGIVAPYNIILVKTYIESIPAELEESAQIDGASYM 211 Query: 185 RSFWSIVVXXXXXXXXXXXXFSAVNQWNSFIDTAFLMTDT-KYYTLQFLLWRYLNESSSL 243 F I+ F AV WNSF+D+ LM + + +TLQ L+ YLN +++L Sbjct: 212 TVFRKIIWPLSKPILATIAIFGAVGNWNSFMDSLILMPNAPQLHTLQHKLYSYLNAATNL 271 Query: 244 A--ALIRSSPDMAASVQNMQTPTSVRMTVTMIVVLPILIVYPFFQRFFVKGIMIGAVKG 300 +++ N+ +++ T++M+ ++PIL+VYPF QR+F KGIM+GAVKG Sbjct: 272 KGVMSSGGGSVSGSALNNILNAKTIKYTISMVTIIPILLVYPFMQRYFEKGIMLGAVKG 330 >A4XJ75.1 A4XJ75_CALS8 Binding-protein-dependent transport systems inner membrane component Length = 296 Score = 196 bits (499), Expect = 1e-48, Method: Compositional matrix adjust. Identities = 107/295 (36%), Positives = 173/295 (58%), Gaps = 2/295 (0%) Query: 6 KKLKPGDALFQGIIYVLFGLFTLSCIYPFYYLFINTISSNDLSAAGDINFYPQQIHFDNY 65 KK GD +F+ Y+L + + +YPF ++ +++ + G I +P++ NY Sbjct: 2 KKKTTGDLVFEVFNYLLMIILAVVTLYPFLHVLAVSLNDPYDTVKGGITIFPRKFTLINY 61 Query: 66 SKVLRISGLSHAALVSVYRTVAGTFLTVGASAFLGYLFTKKEMWGRQIWYRSVVVTMYFS 125 + L + A L++V RTV GTF+ V ++A + Y+ +K+ R+ ++TMY Sbjct: 62 IETLNYPQIPWAVLITVLRTVIGTFVGVLSTAMVAYVINRKDFIARKPVAIMFIITMYVG 121 Query: 126 AGLIPWYITMHNLHLTNNYLAYILPTIITPFNIILVKTFVEAIPPSLEESAAMDGAGVLR 185 GLIP Y+ + L L NN+L YILP +I+PFN+I++K+++E IPP LEESA +DGA Sbjct: 122 GGLIPDYMLIRGLGLMNNFLVYILPGLISPFNVIVIKSYMEGIPPDLEESAMIDGANDFL 181 Query: 186 SFWSIVVXXXXXXXXXXXXFSAVNQWNSFIDT-AFLMTDTKYYTLQFLLWRYLNESSSLA 244 FW I++ F AV WNS+ DT + ++ K TLQ+ L + L+ +++ + Sbjct: 182 IFWKIILPLCAPVIATISLFIAVGHWNSWFDTYLYCSSEPKLTTLQYELQKILSNATA-S 240 Query: 245 ALIRSSPDMAASVQNMQTPTSVRMTVTMIVVLPILIVYPFFQRFFVKGIMIGAVK 299 + + ++ + TP S+RM +T+IV LPIL+VYPF QR+F+ G+ IGAVK Sbjct: 241 SQVDYYSNLDPNRTMKVTPHSLRMAMTIIVTLPILLVYPFIQRYFIHGMTIGAVK 295 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.328 0.140 0.428 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,640,931,053 Number of extensions: 60472225 Number of successful extensions: 239972 Number of sequences better than 1.0e-05: 4239 Number of HSP's gapped: 234555 Number of HSP's successfully gapped: 4242 Length of query: 300 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 165 Effective length of database: 1,788,859,178 Effective search space: 295161764370 Effective search space used: 295161764370 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 131 (55.1 bits)