BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0260.0023.p
         (311 letters)

Database: pfamseq 
           9,421,015 sequences; 3,060,696,203 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

B1D5M5.1 B1D5M5_9BACL 5-carboxymethyl-2-hydroxymuconate Delta-is...   404   e-111
B1DEQ5.1 B1DEQ5_9BACL 5-carboxymethyl-2-hydroxymuconate Delta-is...   351   4e-95
Q5WIZ5.1 Q5WIZ5_BACSK 2-keto-4-pentenoate hydratase/2-oxohepta-3...   292   3e-77
C1PUX7.1 C1PUX7_ALIAC 2-keto-4-pentenoate hydratase/2-oxohepta-3...   276   1e-72
B7DQ46.1 B7DQ46_9BACL 5-carboxymethyl-2-hydroxymuconate Delta-is...   270   1e-70

>B1D5M5.1 B1D5M5_9BACL 5-carboxymethyl-2-hydroxymuconate Delta-isomerase
           EC=5.3.3.10
          Length = 288

 Score =  404 bits (1039), Expect = e-111,   Method: Compositional matrix adjust.
 Identities = 205/312 (65%), Positives = 238/312 (76%), Gaps = 28/312 (8%)

Query: 1   MKLMNFWE---SGNLRLGVKKEEGILDVXXXXXXXXXXXXXXVPATLREVLDGGEAALDS 57
           MKL+NF E   +   RLGV+ ++GI+D                  TL  +L GGEA L  
Sbjct: 1   MKLVNFKEKAAASAYRLGVRTDKGIIDAGH--------------VTLNGLLQGGEAELSK 46

Query: 58  LRKFADSLPYHDEGAKAFYRDETLLEFAPCTADPGKIICIGLNYRRHAEETGMPLPEYPI 117
           LR++ +      +  +  Y DE+ +   P   +PGKIICIGLNYR+HAEET MPLPEYPI
Sbjct: 47  LRQYVE----ESKERQDRYLDESQVVVGPSVPEPGKIICIGLNYRKHAEETNMPLPEYPI 102

Query: 118 LFSKFSNSLSGHGMEVPLPYSTQKVDYEAELGIMIGRTAKNVSEEEALDAVFGYFAANDL 177
           LFSKFSN+L+ HG +VPLP STQKVDYEAEL I+IGRTAKNVS +EALD VFGY  +NDL
Sbjct: 103 LFSKFSNALAAHGEDVPLPRSTQKVDYEAELAIVIGRTAKNVSVDEALDYVFGYSTSNDL 162

Query: 178 SARDLQTRTSQWLGGEXXXXAGKSCDRFAPVGPYVVTEDEVGDPGLLRISCSVNGEVRQD 237
           SARDLQTRTSQWL       AGKSCD+FAPVGPY+V++DEVGDP  LRISC+VNGEVRQD
Sbjct: 163 SARDLQTRTSQWL-------AGKSCDKFAPVGPYLVSKDEVGDPNDLRISCTVNGEVRQD 215

Query: 238 SNTSDMIFDCRQIISYISSCMTLSPGDIILTGTPEGVMMGYPPEKQVYLQPGDEVTVEIE 297
           SNTSDMIF+C++I+SYIS CMTLSPGDIILTGTPEGV+MGYPPEKQVYLQ GD VT+EIE
Sbjct: 216 SNTSDMIFNCKEIVSYISQCMTLSPGDIILTGTPEGVVMGYPPEKQVYLQDGDVVTIEIE 275

Query: 298 KLGRLTNRMVAE 309
           KLG LTNRMV E
Sbjct: 276 KLGALTNRMVVE 287


>B1DEQ5.1 B1DEQ5_9BACL 5-carboxymethyl-2-hydroxymuconate Delta-isomerase
           EC=5.3.3.10
          Length = 300

 Score =  351 bits (901), Expect = 4e-95,   Method: Compositional matrix adjust.
 Identities = 175/310 (56%), Positives = 220/310 (70%), Gaps = 11/310 (3%)

Query: 1   MKLMNFWESGNLRLGVKKEEGILDVXXXXXXXXXXXXXXVPATLREVLDGGEAALDSLRK 60
           MKL+ F E+G  +LG+K + G+LDV              VP  +  V+  G+ A+++L  
Sbjct: 1   MKLLTFLENGQYKLGIKTDLGVLDVASSARELGMAGN--VPTDIHSVIIEGKPAVEALGN 58

Query: 61  FADSLPYHDEGAKAFYRDETLLEFAPCTADPGKIICIGLNYRRHAEETGMPLPEYPILFS 120
            AD     D     ++ +E  L + PC  +P KIICIGLNYRRHAEET   +PEYPILF+
Sbjct: 59  LADKASA-DAHLSPYWLNEDSLNYGPCVTNPQKIICIGLNYRRHAEETNATIPEYPILFN 117

Query: 121 KFSNSLSGHGMEVPLPYSTQKVDYEAELGIMIGRTAKNVSEEE-ALDAVFGYFAANDLSA 179
           KF+N+L+GHG +VPLP  + KVDYEAEL I+IG+ AK + EEE ALD VFGY   ND+SA
Sbjct: 118 KFNNTLTGHGHDVPLPRVSSKVDYEAELAIVIGKQAKYLPEEENALDHVFGYCCVNDISA 177

Query: 180 RDLQTRTSQWLGGEXXXXAGKSCDRFAPVGPYVVTEDEVGDPGLLRISCSVNGEVRQDSN 239
           RDLQ R+ QWL        GKSCD F+P+GPY+VT DEVGDP  L I  +VNGE+RQ+SN
Sbjct: 178 RDLQMRSQQWL-------LGKSCDGFSPLGPYLVTADEVGDPNNLAIRTTVNGEIRQNSN 230

Query: 240 TSDMIFDCRQIISYISSCMTLSPGDIILTGTPEGVMMGYPPEKQVYLQPGDEVTVEIEKL 299
           TSDMIF C++I+SY+S  MTL PGDIIL+GTPEGV++GYPPEKQVYL+ GD VT+ IEKL
Sbjct: 231 TSDMIFHCKEIVSYVSRHMTLLPGDIILSGTPEGVVLGYPPEKQVYLKDGDMVTISIEKL 290

Query: 300 GRLTNRMVAE 309
           G +TNRMV E
Sbjct: 291 GSITNRMVKE 300


>Q5WIZ5.1 Q5WIZ5_BACSK 2-keto-4-pentenoate
           hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase
          Length = 292

 Score =  292 bits (747), Expect = 3e-77,   Method: Compositional matrix adjust.
 Identities = 143/268 (53%), Positives = 190/268 (70%), Gaps = 12/268 (4%)

Query: 40  VPATLREVLDGGEAALDSLRKFADSLPYHDEGAKAFYRDETLLEFAPCTADPGKIICIGL 99
           VPAT+ E+++  E+ L +L  +  +L    E    +        F P   +P KIICIGL
Sbjct: 34  VPATMDELINQPES-LPALEAYMQTL----EQTGPYMHAIESCTFGPAIPNPEKIICIGL 88

Query: 100 NYRRHAEETGMPLPEYPILFSKFSNSLSGHGMEVPLPYSTQKVDYEAELGIMIGRTAKNV 159
           NYRRHA+ETG P PE PILFSKF+N+L+GHG  + +P  TQ +DYE EL I+IG+TAK V
Sbjct: 89  NYRRHADETGSPYPETPILFSKFNNTLTGHGSNIVVPPVTQALDYEVELAIVIGKTAKQV 148

Query: 160 SEEEALDAVFGYFAANDLSARDLQTRTSQWLGGEXXXXAGKSCDRFAPVGPYVVTEDEVG 219
           +E+EAL+ V GY  ANDLSARDLQ +T+QWL        GK+CD F P+GP++VT++++ 
Sbjct: 149 TEDEALNYVLGYCTANDLSARDLQMKTAQWL-------LGKTCDGFCPIGPFLVTKEDIA 201

Query: 220 DPGLLRISCSVNGEVRQDSNTSDMIFDCRQIISYISSCMTLSPGDIILTGTPEGVMMGYP 279
           DP  L +   VNGE++Q SNT+DMIF C +IISY+S   TL PGDI+LTGTPEGV++G P
Sbjct: 202 DPQNLALETRVNGEIKQQSNTADMIFTCAEIISYVSRHFTLKPGDILLTGTPEGVILGLP 261

Query: 280 PEKQVYLQPGDEVTVEIEKLGRLTNRMV 307
             ++ YLQPGD V+V IE+LG LTN++V
Sbjct: 262 EAERRYLQPGDTVSVHIEQLGTLTNKLV 289


>C1PUX7.1 C1PUX7_ALIAC 2-keto-4-pentenoate
           hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase
          Length = 290

 Score =  276 bits (707), Expect = 1e-72,   Method: Compositional matrix adjust.
 Identities = 148/308 (48%), Positives = 200/308 (64%), Gaps = 21/308 (6%)

Query: 1   MKLMNFWESGNLRLGVKKEEGILDVXXXXXXXXXXXXXXVPATLREVLDGGEAALDSLRK 60
           MKL  + E   LRLG+ + + ++DV              VP T+  V+    A    +R+
Sbjct: 3   MKLCTYREGSALRLGLVRGDEVVDVAASAAQLRIS----VPETMEAVI----AEYTHVRR 54

Query: 61  FADSLPYHDEGAKAFYRDETLLEFAPCTADPGKIICIGLNYRRHAEETGMPLPEYPILFS 120
             +++   ++G  A    +  L  AP    P KI+C+GLNYR+HAEE+ MP+P  P+ F+
Sbjct: 55  ALEAI--EEKGVPAGKLSD--LRLAPPVTRPQKILCVGLNYRKHAEESKMPIPSSPVYFA 110

Query: 121 KFSNSLSGHGMEVPLPYSTQKVDYEAELGIMIGRTAKNVSEEEALDAVFGYFAANDLSAR 180
           KF+NSL+ HG E+ +P   ++VDYE EL  +IGR  +NVSEEEAL AVFGY   NDLSAR
Sbjct: 111 KFANSLAAHGDEIAIPEVAREVDYEVELVAVIGRRCRNVSEEEALGAVFGYAIGNDLSAR 170

Query: 181 DLQTRTSQWLGGEXXXXAGKSCDRFAPVGPYVVTEDEVGDPGLLRISCSVNGEVRQDSNT 240
           DLQ R SQWL        GK+ D FAP+GPY+ T DEVGDP  L +   VNGE+RQ+S+T
Sbjct: 171 DLQMRASQWL-------YGKAIDGFAPLGPYIATADEVGDPQRLGLRLYVNGELRQNSST 223

Query: 241 SDMIFDCRQIISYISSCMTLSPGDIILTGTPEGVMMGYPPEKQVYLQPGDEVTVEIEKLG 300
           +DMIF   Q+I+ +S  MTL PGD++ TGTPEGV++G   EK+V+L+PGDE+  EI+ LG
Sbjct: 224 ADMIFSVAQVIADLSRIMTLEPGDLVYTGTPEGVILGR--EKKVWLKPGDEMACEIDGLG 281

Query: 301 RLTNRMVA 308
           RL NR+VA
Sbjct: 282 RLENRLVA 289


>B7DQ46.1 B7DQ46_9BACL 5-carboxymethyl-2-hydroxymuconate Delta-isomerase
           EC=5.3.3.10
          Length = 288

 Score =  270 bits (690), Expect = 1e-70,   Method: Compositional matrix adjust.
 Identities = 145/308 (47%), Positives = 199/308 (64%), Gaps = 21/308 (6%)

Query: 1   MKLMNFWESGNLRLGVKKEEGILDVXXXXXXXXXXXXXXVPATLREVLDGGEAALDSLRK 60
           MKL  + E   LRLG+ + + ++DV              VP T+  V+    A    +R+
Sbjct: 1   MKLCTYREGSALRLGLVRGDEVVDVAASAAQLGIS----VPETMEAVI----AEYTQVRR 52

Query: 61  FADSLPYHDEGAKAFYRDETLLEFAPCTADPGKIICIGLNYRRHAEETGMPLPEYPILFS 120
             +++   ++G  A    +  L  AP    P KI+C+GLNYR+HAEE+ MP+P  P+ F+
Sbjct: 53  ALEAI--EEKGVPAGKLSD--LRLAPPVTRPQKILCVGLNYRKHAEESKMPIPSSPVYFA 108

Query: 121 KFSNSLSGHGMEVPLPYSTQKVDYEAELGIMIGRTAKNVSEEEALDAVFGYFAANDLSAR 180
           KF+NSL+GH  E+ +P   ++VDYE EL  +IGR  +NVSEEEAL AV GY   NDLSAR
Sbjct: 109 KFANSLAGHRDEIAIPEVAREVDYEVELVAVIGRRCRNVSEEEALGAVLGYAIGNDLSAR 168

Query: 181 DLQTRTSQWLGGEXXXXAGKSCDRFAPVGPYVVTEDEVGDPGLLRISCSVNGEVRQDSNT 240
           DLQ R SQWL        GK+ D FAP+GPY+ T DEVGDP  L +   +NGE+RQ+S+T
Sbjct: 169 DLQMRASQWL-------YGKAIDGFAPLGPYIATADEVGDPQRLGLRLYLNGELRQNSST 221

Query: 241 SDMIFDCRQIISYISSCMTLSPGDIILTGTPEGVMMGYPPEKQVYLQPGDEVTVEIEKLG 300
           +DMIF   Q+I+ +S  MTL PGD++ TGTPEGV++G   E++V+L+PGDE+  EI+ LG
Sbjct: 222 ADMIFSVAQVIADLSRIMTLEPGDLVYTGTPEGVILGR--EEKVWLKPGDEMACEIDGLG 279

Query: 301 RLTNRMVA 308
           RL NR+VA
Sbjct: 280 RLENRLVA 287


  Database: pfamseq
    Posted date:  Aug 5, 2010  3:22 PM
  Number of letters in database: 3,060,696,203
  Number of sequences in database:  9,421,015
  
Lambda     K      H
   0.317    0.136    0.402 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 9421015
Number of Hits to DB: 1,855,386,672
Number of extensions: 76927271
Number of successful extensions: 157229
Number of sequences better than 1.0e-05: 2675
Number of HSP's gapped: 151704
Number of HSP's successfully gapped: 2720
Length of query: 311
Length of database: 3,060,696,203
Length adjustment: 135
Effective length of query: 176
Effective length of database: 1,788,859,178
Effective search space: 314839215328
Effective search space used: 314839215328
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 131 (55.1 bits)