BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0258.0009.p (714 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B9M1S6.1 B9M1S6_GEOSF Diguanylate cyclase/phosphodiesterase with... 351 2e-94 Q1K1R0.1 Q1K1R0_DESAC Response regulator receiver modulated digu... 349 6e-94 A1ATC4.1 A1ATC4_PELPD Periplasmic sensor diguanylate cyclase/pho... 346 5e-93 B3K086.1 B3K086_9DELT Diguanylate cyclase/phosphodiesterase 345 8e-93 B5E7Y8.1 B5E7Y8_GEOBB Response regulator receiver modulated digu... 345 9e-93 >B9M1S6.1 B9M1S6_GEOSF Diguanylate cyclase/phosphodiesterase with PAS/PAC sensor(S) Length = 749 Score = 351 bits (900), Expect = 2e-94, Method: Compositional matrix adjust. Identities = 175/427 (40%), Positives = 266/427 (62%), Gaps = 4/427 (0%) Query: 285 RVSKRLYEKLAHYDSLTGIPNRLYFMDEFERLLQSAEPTQT-YTLFFFDLNRFKEINDTL 343 +V++ +LA+YD+LTG+PNR MD E++L A +T L F DL+RFK INDT+ Sbjct: 291 KVNEEQIRRLAYYDNLTGLPNRALLMDRLEQMLTKARRDKTRVVLLFLDLDRFKVINDTM 350 Query: 344 GHSIGDQVIKTLANRLKNGKVPFKLFARTGGDEFVMVFSNLARE-HIPAIAEHISGLISE 402 GH+ GDQ++ +ANRL+ AR GGDEFV++F+ + ++ +A+H+ I+ Sbjct: 351 GHAHGDQLLVQVANRLRQALRSSDTLARIGGDEFVVLFTLEGPQLNVSPLAQHLIDSITS 410 Query: 403 TLLISGAHLSLSTSIGVSLYPEHSLNKDDLLKFADMSMYQAKSQEDANYFIFDWGLREKL 462 ISG + STSIG++ +P+ + + LL+ ADM+MY AK Y + + K Sbjct: 411 VYPISGRDVYASTSIGIATFPDDGGDCNSLLRCADMAMYAAKEGGGKGYHFYSPEMNLKA 470 Query: 463 EQKTMIAKYLHSALEREEFVLHYQPQINAVTGKMIGMEALVRWNHPEKGLIGPGTFITAV 522 + + L ALEREEF L +QP +NA G+++G EAL+RW+ PEKGLI P FI Sbjct: 471 HARMEMETNLRHALEREEFFLEFQPIVNARDGRLVGAEALLRWSDPEKGLIMPDMFIQVA 530 Query: 523 EEAGLMIQLTDWVLREVCRQLCEWRTMGLPLLRTSINISNSWFYNRNLIENLLAVLDQYG 582 EE+G ++ L +WVLR CR++ +WR GLP R S+N+S F N + + +++ + G Sbjct: 531 EESGFIVPLGEWVLRTACRKMKDWREAGLPPFRISVNVSGRQFCQANFTDTVCSIIRETG 590 Query: 583 LETSMLEFEITESTAL--LEEHYPLLQQMRDHGIVVSIDDFGTKYSSLNYLKHFPVNKIK 640 ++ S L E+TE++ + +E L Q+++ I + IDDFG YSSL YL+ FP+++IK Sbjct: 591 MDPSFLALEMTETSLMVDVEATAKALMQLKELDISIVIDDFGAGYSSLGYLQKFPIDRIK 650 Query: 641 IDRTFITGIGVSSIDETIIKSIVYVASQLGYDLIAEGVETQEQLDFLIKHNCPHIQGFLF 700 IDR+FI + + D+ I+++I+ +A++L ++AEGVET EQLDFL + C IQGF F Sbjct: 651 IDRSFIRDLSSHANDKAIVEAIIAMAARLKLQVVAEGVETSEQLDFLKEQGCHEIQGFYF 710 Query: 701 FPPLPAE 707 PL E Sbjct: 711 HRPLSEE 717 >Q1K1R0.1 Q1K1R0_DESAC Response regulator receiver modulated diguanylate cyclase/phosphodiesterase Length = 709 Score = 349 bits (896), Expect = 6e-94, Method: Compositional matrix adjust. Identities = 180/438 (41%), Positives = 268/438 (61%), Gaps = 8/438 (1%) Query: 280 RTRDLRVSKRLYEKLAHYDSLTGIPNRLYFMDEF-ERLLQSAEPTQTYTLFFFDLNRFKE 338 + D+ + E++ HYD+LTG+P R +F D ++ + +T L + DL+RFK Sbjct: 262 QKEDIAYKEMQLEQILHYDTLTGLPQRDFFFDLLHSHMVGCQKNNRTLALLYLDLDRFKN 321 Query: 339 INDTLGHSIGDQVIKTLANRLKNGKVPFKLFARTGGDEFVMVFS---NLAREHIPAIAEH 395 INDTLGH++GDQV++ +A RL+N +R GDEF++ NL+ + +A+ Sbjct: 322 INDTLGHTVGDQVLQEVAQRLQNNIRITDNLSRMSGDEFLITLDPADNLSE--VTQLAQK 379 Query: 396 ISGLISETLLISGAHLSLSTSIGVSLYPEHSLNKDDLLKFADMSMYQAKSQEDANYFIFD 455 +IS + I L+ SIG+SL+P + + + LL AD +MY+AK + + Sbjct: 380 FLSVISVPMKIEHHRFHLTASIGISLFPGDATSPEKLLSCADSAMYRAKESGRDAFRFYK 439 Query: 456 WGLREKLEQKTMIAKYLHSALEREEFVLHYQPQINAVTGKMIGMEALVRWNHPEKGLIGP 515 + EK + + L A+E+E+ LHYQPQ + TG+++GMEALVRWNHPEKG+I P Sbjct: 440 PHMNEKAQGLLWLETELRLAIEQEQLELHYQPQYDLNTGQLVGMEALVRWNHPEKGMIPP 499 Query: 516 GTFITAVEEAGLMIQLTDWVLREVCRQLCEWRTMGLPLLRTSINISNSWFYNRNLIENLL 575 G FI EE L+I L +WVL CRQ+ W MG+ +R ++NIS FY +L + + Sbjct: 500 GEFIPQAEETSLIIPLGEWVLNTACRQIAAWHAMGISPIRMAVNISAVQFYRIDLAQMVA 559 Query: 576 AVLDQYGLETSMLEFEITESTAL--LEEHYPLLQQMRDHGIVVSIDDFGTKYSSLNYLKH 633 +LD YGL ++LE EITES + +E + ++ GI +SIDDFGT YSSL+YLK Sbjct: 560 KLLDHYGLPATLLELEITESVLMDDVEAAAETMNKLTAMGIQLSIDDFGTGYSSLSYLKR 619 Query: 634 FPVNKIKIDRTFITGIGVSSIDETIIKSIVYVASQLGYDLIAEGVETQEQLDFLIKHNCP 693 FP++ +KIDR+FI + ++ D I SI+ +A +G +++AEGVET EQL FL +H C Sbjct: 620 FPISTLKIDRSFINDVTINEQDAAIALSIISLAGHMGIEVLAEGVETVEQLSFLQRHACQ 679 Query: 694 HIQGFLFFPPLPAEDIHK 711 + QG+LF PLPA+DI + Sbjct: 680 YGQGYLFNRPLPAKDIER 697 >A1ATC4.1 A1ATC4_PELPD Periplasmic sensor diguanylate cyclase/phosphodiesterase Length = 876 Score = 346 bits (887), Expect = 5e-93, Method: Compositional matrix adjust. Identities = 180/449 (40%), Positives = 268/449 (59%), Gaps = 13/449 (2%) Query: 274 ERVVSIRTRDLRVSKRLYEKL---AHYDSLTGIPNRLYFMDEFERLLQSAEPT-QTYTLF 329 ++V+ + RD+ KR +L AH+D+LTG+PNR F D + L E + + + Sbjct: 410 QQVICMVLRDISERKRFEGELMHMAHHDALTGLPNRTLFFDRLRQGLYKKERSGKMLAVI 469 Query: 330 FFDLNRFKEINDTLGHSIGDQVIKTLANRLKNGKVPFKLFARTGGDEFVMVFSNLAREHI 389 + DL+RFK INDTLGH GD ++K +A+RL+ +R GGDEF ++ +A Sbjct: 470 YLDLDRFKIINDTLGHHTGDMLLKEVADRLRGVVRKADTISRLGGDEFTIIIDEIA---T 526 Query: 390 PA----IAEHISGLISETLLISGAHLSLSTSIGVSLYPEHSLNKDDLLKFADMSMYQAKS 445 PA +AE I + S + + ++ S+GV+LYP + LLK AD +MY AK Sbjct: 527 PADSLLVAEKILHVFSAPFRLEKHEMFITASMGVTLYPNDGDTAEKLLKNADTAMYHAKE 586 Query: 446 QEDANYFIFDWGLREKLEQKTMIAKYLHSALEREEFVLHYQPQINAVTGKMIGMEALVRW 505 + Y F + ++ ++ + L AL R EF+LHYQP++N TG+++G+EAL+RW Sbjct: 587 EGRNTYQFFSEEMNSRVSERLSMETGLRHALARNEFLLHYQPRVNTTTGRIVGVEALIRW 646 Query: 506 NHPEKGLIGPGTFITAVEEAGLMIQLTDWVLREVCRQLCEWRTMGLPLLRTSINISNSWF 565 P+KGLI P FI EE GL+I + +WVLR C Q W+ G +R S+NIS F Sbjct: 647 QQPQKGLILPDAFIPLAEETGLIIPIGEWVLRNTCTQARAWQEAGFAQMRISVNISCRQF 706 Query: 566 YNRNLIENLLAVLDQYGLETSMLEFEITESTALL--EEHYPLLQQMRDHGIVVSIDDFGT 623 + NL + + +L + GL+ S LE EITES +L E LL ++++ GI ++IDDFGT Sbjct: 707 THDNLPDTIRGILRETGLQPSCLELEITESVIMLNPERAISLLNELKEMGISIAIDDFGT 766 Query: 624 KYSSLNYLKHFPVNKIKIDRTFITGIGVSSIDETIIKSIVYVASQLGYDLIAEGVETQEQ 683 YSSL+ LK P + +KID++F++GI + DET++ +I+ + +G L+AEGVE QEQ Sbjct: 767 GYSSLSLLKRLPADILKIDKSFVSGIPGNKSDETLVATIINLGHNMGLGLVAEGVERQEQ 826 Query: 684 LDFLIKHNCPHIQGFLFFPPLPAEDIHKL 712 L FL + NC +QG+ F PLPAE + L Sbjct: 827 LHFLEERNCQEVQGYYFSKPLPAETLQPL 855 >B3K086.1 B3K086_9DELT Diguanylate cyclase/phosphodiesterase Length = 721 Score = 345 bits (885), Expect = 8e-93, Method: Compositional matrix adjust. Identities = 182/445 (40%), Positives = 267/445 (60%), Gaps = 5/445 (1%) Query: 269 FNRELERVVSIRTRDLRVSKRLYEKLAHYDSLTGIPNRLYFMDEFERLLQSAEPT-QTYT 327 FN L + R + LR + ++LAHYD LT +PNR F D + L AE Q + Sbjct: 247 FNEMLSEIEE-RNQILRQRQAHLQQLAHYDPLTRLPNRTLFYDRLAQALNQAERNRQALS 305 Query: 328 LFFFDLNRFKEINDTLGHSIGDQVIKTLANRLKNGKVPFKLFARTGGDEFVMVFSNLARE 387 + F DL+ FK+INDTLGH GD ++ +ANRL AR GGDEF + N+ Sbjct: 306 VIFIDLDHFKDINDTLGHRTGDLLLIDVANRLSTVVRSCDTVARLGGDEFTVFTQNVMNA 365 Query: 388 HIPAI-AEHISGLISETLLISGAHLSLSTSIGVSLYPEHSLNKDDLLKFADMSMYQAKSQ 446 AI A ++ L ++ + + + ++ SIGV+L+P D+LL AD++MY AK+ Sbjct: 366 DNAAIVAAKLNALFAKPFQLGESEIYVTASIGVTLFPNDGKTVDELLMNADIAMYHAKAC 425 Query: 447 EDANYFIFDWGLREKLEQKTMIAKYLHSALEREEFVLHYQPQINAVTGKMIGMEALVRWN 506 Y +FD + E ++ + L ALEREEFVLHYQP+++ +TG + +EALVRW Sbjct: 426 GKNVYKMFDRKMNEHANERVTLLSDLRLALEREEFVLHYQPKVDLLTGNVTSVEALVRWM 485 Query: 507 HPEKGLIGPGTFITAVEEAGLMIQLTDWVLREVCRQLCEWRTMGLPLLRTSINISNSWFY 566 HP G++ P FI EE+G +++LTDWVLR C Q +W+ L +R ++N+S F Sbjct: 486 HPRLGMLAPDKFIRLSEESGFIVELTDWVLRTACTQAKKWQEQNLGSVRVAVNLSPYHFQ 545 Query: 567 NRNLIENLLAVLDQYGLETSMLEFEITESTALLEEHY--PLLQQMRDHGIVVSIDDFGTK 624 +++ E + +VLD GLE ++LE EITES + + Y L +++R+ G+ +S+DDFGT Sbjct: 546 RQDVKEAIFSVLDATGLEPALLEIEITESALMQNDEYTCALFRELRELGMTISVDDFGTG 605 Query: 625 YSSLNYLKHFPVNKIKIDRTFITGIGVSSIDETIIKSIVYVASQLGYDLIAEGVETQEQL 684 YSSL+YL FP+N +KIDRTFI + SS D+ I+ +I+ +A L ++AEGVET +QL Sbjct: 606 YSSLSYLHRFPINTLKIDRTFILNMMKSSEDQAIVTAIIAMAKSLNMQIVAEGVETVDQL 665 Query: 685 DFLIKHNCPHIQGFLFFPPLPAEDI 709 + L C IQGFL P+ AE + Sbjct: 666 EKLKDQGCHEIQGFLVSRPVAAESV 690 >B5E7Y8.1 B5E7Y8_GEOBB Response regulator receiver modulated diguanylate cyclase/phosphodiesterase with PAS/PAC sensor(S) Length = 704 Score = 345 bits (885), Expect = 9e-93, Method: Compositional matrix adjust. Identities = 181/450 (40%), Positives = 274/450 (60%), Gaps = 19/450 (4%) Query: 282 RDLRVSKRLYEK---LAHYDSLTGIPNRLYFMDEFERLLQSAEP-TQTYTLFFFDLNRFK 337 +D+ KR E+ LA+YD+LTG+PNR +F+ + E+ L A + + F DL+RFK Sbjct: 250 QDITERKRAEEQIRLLAYYDALTGLPNRRFFLQQLEQALVFANRYDRMLAVLFLDLDRFK 309 Query: 338 EINDTLGHSIGDQVIKTLANRL------------KNGKVPFKLFARTGGDEFVMVFSNLA 385 +NDTLGH +GD++++ +A+RL + + P L +R GGDEF ++ S++ Sbjct: 310 LVNDTLGHGVGDRLLQDVADRLLRCVRRGDCLARADEECPPSLVSRLGGDEFTIMLSDIE 369 Query: 386 R-EHIPAIAEHISGLISETLLISGAHLSLSTSIGVSLYPEHSLNKDDLLKFADMSMYQAK 444 + + IA I +S + G + +STSIG+SLYP + DL++ AD +MYQAK Sbjct: 370 HFQDVAKIARRILEAVSVPYSLEGQEVFVSTSIGISLYPFDATTASDLIRNADGAMYQAK 429 Query: 445 SQEDANYFIFDWGLREKLEQKTMIAKYLHSALEREEFVLHYQPQINAVTGKMIGMEALVR 504 Q Y I+D + K ++ ++ LH AL+ EEF+++YQPQ++ +G+++G+EALVR Sbjct: 430 EQGRNGYQIYDESMNAKALERIILESQLHKALKEEEFIVYYQPQVSNQSGRVVGIEALVR 489 Query: 505 WNHPEKGLIGPGTFITAVEEAGLMIQLTDWVLREVCRQLCEWRTMGLPLLRTSINISNSW 564 WN E GL+ PG F+ EE GL+IQ+ WV+RE CRQ W G P + +INIS Sbjct: 490 WNSKELGLVEPGRFLPLAEEIGLVIQIDQWVMREACRQHKLWLDAGFPPVTLAINISGQQ 549 Query: 565 FYNRNLIENLLAVLDQYGLETSMLEFEITEST--ALLEEHYPLLQQMRDHGIVVSIDDFG 622 F L+E + +VL + GL+ +LE E+TE A E LQ ++ G+ ++IDDFG Sbjct: 550 FMKNELLETVTSVLKESGLDPGLLELELTEGVLMAHTERTVKTLQALKGMGVRLAIDDFG 609 Query: 623 TKYSSLNYLKHFPVNKIKIDRTFITGIGVSSIDETIIKSIVYVASQLGYDLIAEGVETQE 682 T +SSL+YLK FP++ +KIDRTFI I D I + + +A + +IAEGVET+ Sbjct: 610 TGFSSLSYLKRFPLDVLKIDRTFINDITTDPDDAAITLATIEMAHTMKLKVIAEGVETKP 669 Query: 683 QLDFLIKHNCPHIQGFLFFPPLPAEDIHKL 712 QLDFL K+ C QG+LF P+P+ ++ +L Sbjct: 670 QLDFLTKNGCDMYQGYLFSKPVPSTELPRL 699 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.138 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,894,908,988 Number of extensions: 156542082 Number of successful extensions: 423873 Number of sequences better than 1.0e-05: 17279 Number of HSP's gapped: 392201 Number of HSP's successfully gapped: 17381 Length of query: 714 Length of database: 3,060,696,203 Length adjustment: 143 Effective length of query: 571 Effective length of database: 1,713,491,058 Effective search space: 978403394118 Effective search space used: 978403394118 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 135 (56.6 bits)