BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0258.0007.p (788 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B0U8I1.1 B0U8I1_METS4 Diguanylate cyclase/phosphodiesterase 325 8e-87 Q088N8.1 Q088N8_SHEFN Diguanylate cyclase/phosphodiesterase with... 321 2e-85 B2JUU8.1 B2JUU8_BURP8 Diguanylate cyclase/phosphodiesterase with... 319 9e-85 Q13LQ1.1 Q13LQ1_BURXL Putative diguanylate cyclase/phosphodieste... 317 3e-84 Q8EJW5.1 Q8EJW5_SHEON Sensory box protein 312 1e-82 >B0U8I1.1 B0U8I1_METS4 Diguanylate cyclase/phosphodiesterase Length = 569 Score = 325 bits (834), Expect = 8e-87, Method: Compositional matrix adjust. Identities = 173/422 (40%), Positives = 241/422 (57%), Gaps = 4/422 (0%) Query: 360 LDPLTHLPNRSLFFAKLNAAIGAAKGSNRQIALVFIDLDHFKTVNDTLGHDFGDAMLQEI 419 LDPLT LPNR L +L AA+ AL+ +DLD FK VNDTLGH GDA+L+++ Sbjct: 150 LDPLTRLPNRLLLRERLGAALDRLARRGEGCALLLVDLDRFKPVNDTLGHPVGDALLEKV 209 Query: 420 AFRLTQAVGTHDVVSRLGGDEFTILLSDIPDSAAISAQLSNIQDALSMPHQIQGHLFYCT 479 A RL V D V+R+GGDEF +L S + D+A +A + D + + I+GHL Sbjct: 210 ADRLRATVRAEDTVARIGGDEFVVLQSAVRDAADTTALAQRLVDLIGRAYMIEGHLVSIG 269 Query: 480 ASIGISIYPQNGDDADYLVKQADLAMLHVKGTGRNNIFQYSEDLEESIRRKKVXXXXXXX 539 AS+GI++ PQ+G D D L+K ADLA+ K GR + ++ ++ ++ Sbjct: 270 ASLGIALAPQDGTDPDRLLKSADLALYRAKRDGRATFRFFEPTMDAQMQARRQLELDMRQ 329 Query: 540 XXXNAEFEVHYQPILNAERLQVHKVEALLRWTSPIYGPVSPAEFIPLAETSGSIVSIGGW 599 EFE+HYQP LN ++ EALLRW P+ G VSPAEFIPLAE G IV IG W Sbjct: 330 ALARREFEIHYQPQLNLLEERLTGCEALLRWRHPVRGMVSPAEFIPLAEEIGLIVPIGDW 389 Query: 600 VLRQVCADMRRFRESGLFLTAAVNISAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 659 V+RQ C D R+ +T AVN+S Sbjct: 390 VIRQACHDAVRWPRP---ITVAVNVSPAQFKGDRLVDTVVSALARSGLPAPRLEIEITEG 446 Query: 660 VLVNGDS-ILESLQQLRAHGFRISLDDFGTGFSSLSYLRRFPVDVIKIDRSFVSEMTPHP 718 +L+ D+ L +L +LR G R+S+DDFGTG+SSLSYLR FP D IKIDRSFV +M P Sbjct: 447 LLLQDDAQTLRTLHRLRDLGVRVSMDDFGTGYSSLSYLRAFPFDKIKIDRSFVRDMAQKP 506 Query: 719 QGDVLVKAIIELSHNLGLSVVSEGIELQEQFDMLRSLGSDELQGFYISKPVRAGEIRSFL 778 GD +++AI L +LG++ V+EG+E EQ +R+ G ++QG+ +S+P+ A ++ + L Sbjct: 507 DGDAIIRAIAGLGRSLGMTTVAEGVETPEQMRRIRAEGCTDVQGYLVSRPIPADDLLALL 566 Query: 779 SR 780 +R Sbjct: 567 AR 568 >Q088N8.1 Q088N8_SHEFN Diguanylate cyclase/phosphodiesterase with PAS/PAC sensor(S) Length = 1486 Score = 321 bits (823), Expect = 2e-85, Method: Compositional matrix adjust. Identities = 168/425 (39%), Positives = 242/425 (56%), Gaps = 1/425 (0%) Query: 360 LDPLTHLPNRSLFFAKLNAAIGAAKGSNRQIALVFIDLDHFKTVNDTLGHDFGDAMLQEI 419 DPLT LPNR+LF +LN A+ A +N +AL+F+DLD FK +ND++GH GD +L+ + Sbjct: 1057 FDPLTGLPNRTLFQDRLNQAVTKAHRANSIVALLFLDLDRFKHINDSMGHHIGDLLLKAV 1116 Query: 420 AFRLTQAVGTHDVVSRLGGDEFTILLSDIPDSAAISAQLSNIQDALSMPHQIQGHLFYCT 479 A RL AV D V+RLGGDEFTI+L + + A + + A P + + + Sbjct: 1117 AHRLQNAVRDGDTVARLGGDEFTIILEGVAKTKAATIIAEKVLHAFQTPFLLDDKILTIS 1176 Query: 480 ASIGISIYPQNGDDADYLVKQADLAMLHVKGTGRNNIFQYSEDLEESIRRKKVXXXXXXX 539 SIGIS+YP++ DD L+K AD AM H K GRNN Y+E L + R Sbjct: 1177 PSIGISLYPEDADDTTSLIKYADTAMYHAKSLGRNNFQFYTEQLNQYATRHVQLEAGLKK 1236 Query: 540 XXXNAEFEVHYQPILNAERLQVHKVEALLRWTSPIYGPVSPAEFIPLAETSGSIVSIGGW 599 + E + YQP + + ++ +EAL+RW SP G +SP EFIPLAE +G I IG W Sbjct: 1237 AIQDNELYLVYQPKYDVKTEKIIGLEALMRWESPELGFISPVEFIPLAEETGIINQIGHW 1296 Query: 600 VLRQVCADMRRFRESGL-FLTAAVNISAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 658 ++Q C + ++ + G ++ AVN+SA Sbjct: 1297 AIQQACNQLAQWHQQGFEHISIAVNLSARQLKADIISTIEVALAVSGLPASALELELTES 1356 Query: 659 XVLVNGDSILESLQQLRAHGFRISLDDFGTGFSSLSYLRRFPVDVIKIDRSFVSEMTPHP 718 ++ N + L QL+A G I++DDFGTG+SSLSYL+RFP+D +KIDR FV ++T P Sbjct: 1357 MIMGNPQDSVTVLSQLKALGLTIAIDDFGTGYSSLSYLKRFPIDTLKIDREFVRDITEDP 1416 Query: 719 QGDVLVKAIIELSHNLGLSVVSEGIELQEQFDMLRSLGSDELQGFYISKPVRAGEIRSFL 778 + AII L+H+L L+VV+EG+E Q Q + LR G D++QGF +SKP+ A + + L Sbjct: 1417 DDAAITSAIITLAHSLELNVVAEGVETQAQLNFLREGGCDQVQGFLLSKPLSAVDCLALL 1476 Query: 779 SRKKS 783 ++ KS Sbjct: 1477 TQDKS 1481 >B2JUU8.1 B2JUU8_BURP8 Diguanylate cyclase/phosphodiesterase with PAS/PAC sensor(S) Length = 1061 Score = 319 bits (817), Expect = 9e-85, Method: Compositional matrix adjust. Identities = 173/462 (37%), Positives = 263/462 (56%), Gaps = 5/462 (1%) Query: 325 IISTGRDEFSDVEHEFNRMIDSXXXXXXXXXXXSML--DPLTHLPNRSLFFAKLNAAIGA 382 +IS RD+ V H +ID + D LT LPNR+LF +L + Sbjct: 594 MISAVRDKRGSVSHYICTLIDITDRKKSEARIEFLAEHDVLTELPNRALFTKRLGVVLDN 653 Query: 383 AKGSNRQIALVFIDLDHFKTVNDTLGHDFGDAMLQEIAFRLTQAVGTHDVVSRLGGDEFT 442 AK + +++A++FIDLD FK +ND+LGH GD +L+ ++ RL QAV + D VSRLGGDEFT Sbjct: 654 AKHAQQRVAVLFIDLDRFKDINDSLGHHIGDGLLRSVSRRLVQAVRSGDTVSRLGGDEFT 713 Query: 443 ILLSDIPDSAAISAQL-SNIQDALSMPHQIQGHLFYCTASIGISIYPQNGDDADYLVKQA 501 ++L+D +A ++ + + + PH I G S+G+++YP +G D + L++ A Sbjct: 714 MVLADAGSAADVARTIDERLLPLVREPHAIGGVTLQVACSVGVALYPDDGADIETLMQNA 773 Query: 502 DLAMLHVKGTGRNNIFQYSEDLEESIRRKKVXXXXXXXXXXNAEFEVHYQPILNAERLQV 561 D AM K GRN + +S D+ E R++ E + YQP L+A+ + Sbjct: 774 DAAMYQAKAAGRNLVKFFSPDMAERARQRLALEACMRTAIERRELRLAYQPCLDAQTGAL 833 Query: 562 HKVEALLRWTSPIYGPVSPAEFIPLAETSGSIVSIGGWVLRQVCADMRRFRESGLF-LTA 620 VE LLRWT P G V+P++FIP+AE + I+ IG WV+ Q C + R+R + + L Sbjct: 834 VSVEGLLRWTHPQLGAVTPSQFIPIAEETRLIIPIGAWVIDQACRQLARWRNAEMGELRM 893 Query: 621 AVNISAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVLVN-GDSILESLQQLRAHGF 679 ++N+SA VL++ ++ L+++ +RA G Sbjct: 894 SINLSAIQLRDADLVETLRHSLVTHGVAPQRLELEITETVLMDSAENYLDAICAIRALGV 953 Query: 680 RISLDDFGTGFSSLSYLRRFPVDVIKIDRSFVSEMTPHPQGDVLVKAIIELSHNLGLSVV 739 ++SLDDFGTG+SSLSYL RFP+D +KIDR+FV +M P ++KAIIEL H LGL VV Sbjct: 954 KLSLDDFGTGYSSLSYLNRFPLDRLKIDRAFVRDMLDAPADLAVIKAIIELGHELGLRVV 1013 Query: 740 SEGIELQEQFDMLRSLGSDELQGFYISKPVRAGEIRSFLSRK 781 +EG+E +++ + LRS+G DELQGF S + A E+ ++ ++ Sbjct: 1014 AEGVESEDEANTLRSIGCDELQGFLYSAALSAEELETWAQQR 1055 >Q13LQ1.1 Q13LQ1_BURXL Putative diguanylate cyclase/phosphodiesterase(GGDEF & EAL domains) with PAS/PAC sensor(S) Length = 1059 Score = 317 bits (812), Expect = 3e-84, Method: Compositional matrix adjust. Identities = 170/463 (36%), Positives = 265/463 (57%), Gaps = 5/463 (1%) Query: 325 IISTGRDEFSDVEHEFNRMIDSXXXXXXXXXXXSML--DPLTHLPNRSLFFAKLNAAIGA 382 ++S RD +++ H +ID + D LT LPNR LF +L +G Sbjct: 595 MVSAVRDRRANLSHYICTLIDITDRKKSEARIQFLAEHDVLTELPNRELFVKRLGVVLGN 654 Query: 383 AKGSNRQIALVFIDLDHFKTVNDTLGHDFGDAMLQEIAFRLTQAVGTHDVVSRLGGDEFT 442 A+ + +++A++FIDLD FK +ND+LGH GD++L+ ++ RL QAV T+D VSRLGGDEFT Sbjct: 655 ARRTGQRVAVLFIDLDRFKNINDSLGHHIGDSLLRSVSRRLLQAVRTNDTVSRLGGDEFT 714 Query: 443 ILLSDIPDSAAISAQLSN-IQDALSMPHQIQGHLFYCTASIGISIYPQNGDDADYLVKQA 501 ++L+ + ++A ++ + + + PH++ G + S+G++++P +G+D + L++ A Sbjct: 715 VILNSVSNAADVTRTIEERLLPLVRQPHEVGGVTLQVSCSVGVALFPDDGEDIETLMQNA 774 Query: 502 DLAMLHVKGTGRNNIFQYSEDLEESIRRKKVXXXXXXXXXXNAEFEVHYQPILNAERLQV 561 D AM KG GRN + ++ D+ E R + EF + YQP ++A ++ Sbjct: 775 DAAMYQAKGAGRNLVKFFAPDIAERTRYRLTLEACLRSAIAMHEFRLEYQPCVDAHTGEL 834 Query: 562 HKVEALLRWTSPIYGPVSPAEFIPLAETSGSIVSIGGWVLRQVCADMRRFRESGLF-LTA 620 VE LLRW SP G VSPA+FIP+AE + I+ IG WV+ + C + + GL L Sbjct: 835 VAVEGLLRWNSPQIGIVSPAQFIPVAEDTRLIIPIGAWVINEACQQIAAWDAEGLTPLRV 894 Query: 621 AVNISAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVLV-NGDSILESLQQLRAHGF 679 ++N+SA VL+ N ++ L + +RA G Sbjct: 895 SINLSAIQLRDPDLIGTLADSLARHGVAPWRLELEITETVLMDNAENYLTMINAIRALGV 954 Query: 680 RISLDDFGTGFSSLSYLRRFPVDVIKIDRSFVSEMTPHPQGDVLVKAIIELSHNLGLSVV 739 ++SLDDFG G+SSLSYL RFP+D +KIDR+FV +M +V+AIIEL H LGL VV Sbjct: 955 KLSLDDFGIGYSSLSYLNRFPLDRLKIDRAFVLDMLDARADLAIVRAIIELGHELGLRVV 1014 Query: 740 SEGIELQEQFDMLRSLGSDELQGFYISKPVRAGEIRSFLSRKK 782 +EG+E + Q +LR++G DELQGF SK + ++ S+ + +K Sbjct: 1015 AEGVESEHQARILRTIGCDELQGFLFSKALSPADLWSWTNARK 1057 >Q8EJW5.1 Q8EJW5_SHEON Sensory box protein Length = 1515 Score = 312 bits (799), Expect = 1e-82, Method: Compositional matrix adjust. Identities = 169/423 (39%), Positives = 233/423 (55%), Gaps = 1/423 (0%) Query: 360 LDPLTHLPNRSLFFAKLNAAIGAAKGSNRQIALVFIDLDHFKTVNDTLGHDFGDAMLQEI 419 D LT LPNR+LF +LN AI A SN +AL+F+DLD FK +ND++GH GD +L+ + Sbjct: 1084 FDTLTGLPNRTLFQDRLNHAISQAHRSNNIVALLFLDLDRFKHINDSMGHHIGDLLLKAV 1143 Query: 420 AFRLTQAVGTHDVVSRLGGDEFTILLSDIPDSAAISAQLSNIQDALSMPHQIQGHLFYCT 479 A RL AV D V+RLGGDEFTI+L + + A + + A P + + Sbjct: 1144 AHRLQSAVREGDTVARLGGDEFTIILEGVAKTKAATLISEKVLKAFQAPFLLDDKSLNIS 1203 Query: 480 ASIGISIYPQNGDDADYLVKQADLAMLHVKGTGRNNIFQYSEDLEESIRRKKVXXXXXXX 539 SIGIS+YP + D D L+K AD AM H K GRNN Y+ L E R Sbjct: 1204 TSIGISLYPNDAKDVDSLIKFADTAMYHAKALGRNNFQFYTNKLNEMATRHMQLETGLKQ 1263 Query: 540 XXXNAEFEVHYQPILNAERLQVHKVEALLRWTSPIYGPVSPAEFIPLAETSGSIVSIGGW 599 + E + YQP + +EALLRW GP+SPAEFIPLAE +G I IG W Sbjct: 1264 AISHNELSLVYQPKFCLRNGSLTGLEALLRWQHSELGPISPAEFIPLAEETGMINQIGHW 1323 Query: 600 VLRQVCADMRRFRESGLF-LTAAVNISAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 658 V+ Q C + + E G ++ AVN+SA Sbjct: 1324 VINQSCQQLAEWNELGFSDISMAVNLSARQLKADIISTIEVALAVSGLPAKALELELTES 1383 Query: 659 XVLVNGDSILESLQQLRAHGFRISLDDFGTGFSSLSYLRRFPVDVIKIDRSFVSEMTPHP 718 ++ N + L +L+A G I++DDFGTG+SSLSYL+RFP+D +KIDR FV ++T P Sbjct: 1384 MIMGNPQESVNILSKLKALGLTIAVDDFGTGYSSLSYLKRFPIDTLKIDREFVRDITNDP 1443 Query: 719 QGDVLVKAIIELSHNLGLSVVSEGIELQEQFDMLRSLGSDELQGFYISKPVRAGEIRSFL 778 + AII L+H+L L+VV+EG+E QEQ + L G D++QGF +SKP+ A + ++ L Sbjct: 1444 DDAAITSAIIALAHSLELNVVAEGVETQEQLNFLALQGCDQVQGFLLSKPLSAQDCQTLL 1503 Query: 779 SRK 781 ++ Sbjct: 1504 QQR 1506 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.137 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,979,048,191 Number of extensions: 152727729 Number of successful extensions: 401261 Number of sequences better than 1.0e-05: 17272 Number of HSP's gapped: 384376 Number of HSP's successfully gapped: 21613 Length of query: 788 Length of database: 3,060,696,203 Length adjustment: 144 Effective length of query: 644 Effective length of database: 1,704,070,043 Effective search space: 1097421107692 Effective search space used: 1097421107692 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 135 (56.6 bits)