BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0258.0004.p (455 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DCR9.1 B1DCR9_9BACL Sodium:dicarboxylate symporter 629 e-178 C1I2W9.1 C1I2W9_9CLOT Transmembrane symporter 306 4e-81 B1RH41.1 B1RH41_CLOPE Sodium:dicarboxylate symporter family 305 5e-81 Q0SU65.1 Q0SU65_CLOPS Sodium:dicarboxylate symporter family protein 303 2e-80 Q0TRV3.1 Q0TRV3_CLOP1 Sodium:dicarboxylate symporter family 303 2e-80 >B1DCR9.1 B1DCR9_9BACL Sodium:dicarboxylate symporter Length = 455 Score = 629 bits (1621), Expect = e-178, Method: Compositional matrix adjust. Identities = 326/456 (71%), Positives = 355/456 (77%), Gaps = 2/456 (0%) Query: 1 MNNIDNNLLSAIETNWQGFVISIFVFGILFLLARKRIGFGTRVXXXXXXXXXXXXXXXXX 60 M+ IDNNLL AIETNW FV +IFVF IL+ LARKR+GFGTRV Sbjct: 1 MDKIDNNLLLAIETNWYSFVTAIFVFAILYFLARKRVGFGTRVLVGLGLGLVVGILFQSL 60 Query: 61 D-LETEAIKTFGSIYVSLIRMLVIPLVFILVLNSISSLTNLEYLRKIGIKTFAWFLGTTG 119 D LE + I TFGSIY+SLIRMLVIPLVF+LVLNSI+SLT+L+YLRKIG KTFAWFLGTTG Sbjct: 61 DSLEIKGITTFGSIYISLIRMLVIPLVFVLVLNSIASLTSLDYLRKIGFKTFAWFLGTTG 120 Query: 120 VASIIGLSVALLFNPGKGIQQAVPEGFEAREIPTFSQVILDLVPSNPVNEAATGKVVPXX 179 +AS+IGL AL+FNPGKGIQQ REIPTFS VIL LVPSNPV+EAA GKVVP Sbjct: 121 IASVIGLLTALVFNPGKGIQQPAASDAATREIPTFSDVILSLVPSNPVSEAADGKVVPIL 180 Query: 180 XXXXXXXXXXXKVGSKKPEAVKPVRDLIESLTTVLHQVVKFVIRLTPYGVFALIAGITAR 239 KVGSK EAVKPVRDLI+S T VLHQVVKFVIRLTPYGVFALIAG+ A+ Sbjct: 181 IFAIFLAVAIIKVGSKNEEAVKPVRDLIKSATQVLHQVVKFVIRLTPYGVFALIAGVAAK 240 Query: 240 YGWETLQELGSVIITSYVALIIHXXXXXXXXXXXXAKVNPLRFFRKIYPTLTVAFTTRSS 299 YGW+TLQELGSVI+TSYVALIIH AKVNPL+FF+K+YPT+ VAFTTRSS Sbjct: 241 YGWDTLQELGSVIVTSYVALIIHFVLVFGGLVLFVAKVNPLKFFKKVYPTVAVAFTTRSS 300 Query: 300 YATLPVNLEVITKRLHVSPRIASFVAPLGASVNFNGCGGIWPAIVAVFTAQVFGIHLTGT 359 YATLP+NLEVITKRLHVSP+IASFVAPLGASVNFNGCGG+WPAIV VFT+Q FGI L T Sbjct: 301 YATLPINLEVITKRLHVSPKIASFVAPLGASVNFNGCGGVWPAIVVVFTSQAFGIELGLT 360 Query: 360 DYVTLVLVSIISSIGVAGVPGPAVISTTVVLTALGLPLEGIAIVAGVEALIDMGRXXXXX 419 DYV LVLVSIISSIGVAGVPGPAVISTTVVLTALGLPLE +AIVAGVEALIDM R Sbjct: 361 DYVVLVLVSIISSIGVAGVPGPAVISTTVVLTALGLPLEALAIVAGVEALIDMARTAVNA 420 Query: 420 XXXXXXXXXXXXSEGEFDREAFNRGDDDEEILLNAV 455 SEGEFDREAFN D+DEEI LNAV Sbjct: 421 TGTSVTALLVANSEGEFDREAFNN-DNDEEIALNAV 455 >C1I2W9.1 C1I2W9_9CLOT Transmembrane symporter Length = 433 Score = 306 bits (783), Expect = 4e-81, Method: Compositional matrix adjust. Identities = 157/344 (45%), Positives = 225/344 (65%) Query: 71 GSIYVSLIRMLVIPLVFILVLNSISSLTNLEYLRKIGIKTFAWFLGTTGVASIIGLSVAL 130 G Y+SLI+M+V+PLV + ++ SI L NL+ L+ IGIKT LGTTGVA+IIG+ + Sbjct: 58 GKGYISLIKMIVVPLVMVSIITSIIRLKNLDTLKSIGIKTLGILLGTTGVAAIIGIIIGK 117 Query: 131 LFNPGKGIQQAVPEGFEAREIPTFSQVILDLVPSNPVNEAATGKVVPXXXXXXXXXXXXX 190 +F G+G+ EGF+AREIP+FS+V+LD +PSNPV +++P Sbjct: 118 IFKLGQGLSFVGAEGFKAREIPSFSKVLLDFLPSNPVASVVNNQIIPIVIFSLFIAIALV 177 Query: 191 KVGSKKPEAVKPVRDLIESLTTVLHQVVKFVIRLTPYGVFALIAGITARYGWETLQELGS 250 +K PE VKP +D I S ++ + + V++ PYGVFALI A G +T++ L + Sbjct: 178 IEDNKNPEKVKPFKDFILSTYELVLSITRIVLKFIPYGVFALITTAAATNGMDTIKSLIN 237 Query: 251 VIITSYVALIIHXXXXXXXXXXXXAKVNPLRFFRKIYPTLTVAFTTRSSYATLPVNLEVI 310 VI+ Y+A I+ A+ NPL+FF+ I+P +AFT++SSY TLPV ++ + Sbjct: 238 VILAVYIACILQIVLVHTPLIAFVARKNPLKFFKDIFPAQVIAFTSQSSYGTLPVTIKSL 297 Query: 311 TKRLHVSPRIASFVAPLGASVNFNGCGGIWPAIVAVFTAQVFGIHLTGTDYVTLVLVSII 370 + VS IASFVAPLG+++ N CGG++PAIVA+F A VF + +T Y +VL ++I Sbjct: 298 VENAKVSENIASFVAPLGSTIGMNACGGLYPAIVAIFVANVFNVDMTMYHYFLIVLTTVI 357 Query: 371 SSIGVAGVPGPAVISTTVVLTALGLPLEGIAIVAGVEALIDMGR 414 SSIG+AGVPG A +STTVVL+ LGLP+EG+A+V V+A+IDM R Sbjct: 358 SSIGIAGVPGAATMSTTVVLSTLGLPIEGMAMVMAVDAIIDMMR 401 >B1RH41.1 B1RH41_CLOPE Sodium:dicarboxylate symporter family Length = 435 Score = 305 bits (782), Expect = 5e-81, Method: Compositional matrix adjust. Identities = 158/348 (45%), Positives = 226/348 (64%) Query: 67 IKTFGSIYVSLIRMLVIPLVFILVLNSISSLTNLEYLRKIGIKTFAWFLGTTGVASIIGL 126 + T G Y+SLI+M+V+PLV ++ SI L NL+ L+ IG+KTF LGTTG A++IG+ Sbjct: 55 LDTVGKAYMSLIKMIVVPLVVTSLITSIVRLENLDTLKSIGLKTFTVLLGTTGAAALIGI 114 Query: 127 SVALLFNPGKGIQQAVPEGFEAREIPTFSQVILDLVPSNPVNEAATGKVVPXXXXXXXXX 186 VA N G+G++ E F+AREIP FS+V++D++PSNP+ K++P Sbjct: 115 IVASSLNLGQGLRFIGAENFKAREIPGFSKVLIDMLPSNPLAAIVENKIIPIVIFSMFIA 174 Query: 187 XXXXKVGSKKPEAVKPVRDLIESLTTVLHQVVKFVIRLTPYGVFALIAGITARYGWETLQ 246 + E KP +D I S ++ ++ K V+R+ PYGVFALIA A+ G +TL Sbjct: 175 IALVIEDNTNKEKAKPFKDFILSAYDIVLRITKMVLRIIPYGVFALIATAAAKNGMDTLM 234 Query: 247 ELGSVIITSYVALIIHXXXXXXXXXXXXAKVNPLRFFRKIYPTLTVAFTTRSSYATLPVN 306 L VI+ Y+A + A++NPL+FF+ I+P VAFT++SSY TLPV Sbjct: 235 SLIWVILAVYIAAFLQFLFVYTPLISFVARMNPLKFFKGIFPAQVVAFTSQSSYGTLPVT 294 Query: 307 LEVITKRLHVSPRIASFVAPLGASVNFNGCGGIWPAIVAVFTAQVFGIHLTGTDYVTLVL 366 ++ + + + VS IASFVAPLG+++ NGCGG +PAIVA+F A VF + LT Y+ +VL Sbjct: 295 IKSLVEGVGVSENIASFVAPLGSTIGLNGCGGFYPAIVAIFAANVFNVELTSYSYILIVL 354 Query: 367 VSIISSIGVAGVPGPAVISTTVVLTALGLPLEGIAIVAGVEALIDMGR 414 +IISSIG+AGVPG A +STTV+L ALGLP+E +A+V V+++IDM R Sbjct: 355 TAIISSIGIAGVPGSATMSTTVMLAALGLPIEALAMVIAVDSIIDMIR 402 >Q0SU65.1 Q0SU65_CLOPS Sodium:dicarboxylate symporter family protein Length = 435 Score = 303 bits (776), Expect = 2e-80, Method: Compositional matrix adjust. Identities = 158/348 (45%), Positives = 225/348 (64%) Query: 67 IKTFGSIYVSLIRMLVIPLVFILVLNSISSLTNLEYLRKIGIKTFAWFLGTTGVASIIGL 126 + T G Y+SLI+M+V+PLV ++ SI L NL+ L+ IG+KTF LGTTG A+ IG+ Sbjct: 55 LDTVGKAYMSLIKMIVVPLVVTSLITSIVRLENLDTLKSIGLKTFTVLLGTTGAAAFIGI 114 Query: 127 SVALLFNPGKGIQQAVPEGFEAREIPTFSQVILDLVPSNPVNEAATGKVVPXXXXXXXXX 186 VA N G+G++ E F+AREIP FS+V++D++PSNP+ K++P Sbjct: 115 IVASSLNLGQGLRFIGAENFKAREIPGFSKVLIDMLPSNPLAAIVENKIIPIVIFSMFIA 174 Query: 187 XXXXKVGSKKPEAVKPVRDLIESLTTVLHQVVKFVIRLTPYGVFALIAGITARYGWETLQ 246 + E KP +D I S ++ ++ K V+R+ PYGVFALIA A+ G +TL Sbjct: 175 IALVIEDNTNKEKAKPFKDFILSAYDIVLRITKMVLRIIPYGVFALIATAAAKNGMDTLM 234 Query: 247 ELGSVIITSYVALIIHXXXXXXXXXXXXAKVNPLRFFRKIYPTLTVAFTTRSSYATLPVN 306 L VI+ Y+A + A++NPL+FF+ I+P VAFT++SSY TLPV Sbjct: 235 SLIWVILAVYIAAFLQFLFVYTPLISFVARMNPLKFFKGIFPAQVVAFTSQSSYGTLPVT 294 Query: 307 LEVITKRLHVSPRIASFVAPLGASVNFNGCGGIWPAIVAVFTAQVFGIHLTGTDYVTLVL 366 ++ + + + VS IASFVAPLG+++ NGCGG +PAIVA+F A VF + LT Y+ +VL Sbjct: 295 IKSLVEGVGVSENIASFVAPLGSTIGLNGCGGFYPAIVAIFAANVFNVELTIYSYILIVL 354 Query: 367 VSIISSIGVAGVPGPAVISTTVVLTALGLPLEGIAIVAGVEALIDMGR 414 +IISSIG+AGVPG A +STTV+L ALGLP+E +A+V V+++IDM R Sbjct: 355 TAIISSIGIAGVPGSATMSTTVMLAALGLPIEALAMVIAVDSIIDMIR 402 >Q0TRV3.1 Q0TRV3_CLOP1 Sodium:dicarboxylate symporter family Length = 435 Score = 303 bits (776), Expect = 2e-80, Method: Compositional matrix adjust. Identities = 158/348 (45%), Positives = 225/348 (64%) Query: 67 IKTFGSIYVSLIRMLVIPLVFILVLNSISSLTNLEYLRKIGIKTFAWFLGTTGVASIIGL 126 + T G Y+SLI+M+V+PLV ++ SI L NL+ L+ IG+KTF LGTTG A+ IG+ Sbjct: 55 LDTVGKAYMSLIKMIVVPLVVTSLITSIVRLENLDTLKSIGLKTFTVLLGTTGAAAFIGI 114 Query: 127 SVALLFNPGKGIQQAVPEGFEAREIPTFSQVILDLVPSNPVNEAATGKVVPXXXXXXXXX 186 VA N G+G++ E F+AREIP FS+V++D++PSNP+ K++P Sbjct: 115 IVASSLNLGQGLRFIGAENFKAREIPGFSKVLIDMLPSNPLAAIVENKIIPIVIFSMFIA 174 Query: 187 XXXXKVGSKKPEAVKPVRDLIESLTTVLHQVVKFVIRLTPYGVFALIAGITARYGWETLQ 246 + E KP +D I S ++ ++ K V+R+ PYGVFALIA A+ G +TL Sbjct: 175 IALVIEDNTNKEKAKPFKDFILSAYDIVLRITKMVLRIIPYGVFALIATAAAKNGMDTLM 234 Query: 247 ELGSVIITSYVALIIHXXXXXXXXXXXXAKVNPLRFFRKIYPTLTVAFTTRSSYATLPVN 306 L VI+ Y+A + A++NPL+FF+ I+P VAFT++SSY TLPV Sbjct: 235 SLIWVILAVYIAAFLQFLFVYTPLISFVARMNPLKFFKGIFPAQVVAFTSQSSYGTLPVT 294 Query: 307 LEVITKRLHVSPRIASFVAPLGASVNFNGCGGIWPAIVAVFTAQVFGIHLTGTDYVTLVL 366 ++ + + + VS IASFVAPLG+++ NGCGG +PAIVA+F A VF + LT Y+ +VL Sbjct: 295 IKSLVEGVGVSENIASFVAPLGSTIGLNGCGGFYPAIVAIFAANVFNVELTIYSYILIVL 354 Query: 367 VSIISSIGVAGVPGPAVISTTVVLTALGLPLEGIAIVAGVEALIDMGR 414 +IISSIG+AGVPG A +STTV+L ALGLP+E +A+V V+++IDM R Sbjct: 355 TAIISSIGIAGVPGSATMSTTVMLAALGLPIEALAMVIAVDSIIDMIR 402 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.324 0.141 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,207,745,817 Number of extensions: 79805591 Number of successful extensions: 220301 Number of sequences better than 1.0e-05: 3412 Number of HSP's gapped: 211051 Number of HSP's successfully gapped: 3416 Length of query: 455 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 316 Effective length of database: 1,751,175,118 Effective search space: 553371337288 Effective search space used: 553371337288 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 133 (55.8 bits)