BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0256.0067.p (247 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KKV6.1 B3KKV6_9BACI Polynucleotide adenylyltransferase region 262 2e-68 B1DM05.1 B1DM05_9BACL Polynucleotide adenylyltransferase region 254 6e-66 B4BM25.1 B4BM25_9BACI Polynucleotide adenylyltransferase region 229 2e-58 A4IQ63.1 CCA_GEOTN CCA-adding enzyme EC=2.7.7.25 EC=2.7.7.21 229 2e-58 C0XLT2.1 C0XLT2_LACHI tRNA nucleotidyltransferase/poly(A) polyme... 229 2e-58 >B3KKV6.1 B3KKV6_9BACI Polynucleotide adenylyltransferase region Length = 246 Score = 262 bits (669), Expect = 2e-68, Method: Compositional matrix adjust. Identities = 129/222 (58%), Positives = 160/222 (72%), Gaps = 3/222 (1%) Query: 3 WTMAPSGMXXXXXXXXXXXXEQGYEAYFVGGCVRDELLGRPVHDMDLTTSAWPEEVMAIF 62 W A M G+EAY+VGGCVRDE +GR V DMD+TTSA PE ++F Sbjct: 10 WRNADPDMAERSADVIRTLLRHGHEAYWVGGCVRDEYMGRRVSDMDITTSALPEVTCSVF 69 Query: 63 PRCVPTGLAHGTITVLQDGWSFEVTTYRTESGYADHRRPEQVLFVSDVKEDLRRRDFTIN 122 P+ +PTG+ HGT+TVL +G SFEVTTYR ESGY DHR P +V FV +VKEDLRRRDFT+N Sbjct: 70 PKVIPTGIKHGTVTVLMEGQSFEVTTYRVESGYEDHRHPTEVAFVGEVKEDLRRRDFTMN 129 Query: 123 AMCRGLDGVVVDPYGGQEDSERRLIXXXXCVGDAEARFGEDALRMLRCVRFASVLDFSIA 182 AM RG+DG VDP+GG+ED +R++I CVG+A RF EDALRM+RC+RFASV FSIA Sbjct: 130 AMARGIDGSWVDPFGGREDIDRKVI---RCVGEARLRFREDALRMVRCIRFASVFGFSIA 186 Query: 183 KNTWRGLLRQRDKLAHIAVERVRAELERIVLGPHPQRGLGLV 224 TWRG+L +RD L IA+ER+R+E E+I+ GP P +G V Sbjct: 187 LRTWRGILSERDTLGWIAMERIRSEFEKILAGPSPAARIGDV 228 >B1DM05.1 B1DM05_9BACL Polynucleotide adenylyltransferase region Length = 417 Score = 254 bits (648), Expect = 6e-66, Method: Compositional matrix adjust. Identities = 128/225 (56%), Positives = 159/225 (70%), Gaps = 10/225 (4%) Query: 23 EQGYEAYFVGGCVRDELLGRPVHDMDLTTSAWPEEVMAIFPRCVPTGLAHGTITVLQDGW 82 +QG+EA FVGGCVRD +G P+ D+D+ TSA PE+VM+IFPRC+PTGL HGT+TVL DG Sbjct: 23 QQGFEAVFVGGCVRDTAMGLPIKDVDIATSARPEQVMSIFPRCIPTGLQHGTVTVLHDGG 82 Query: 83 SFEVTTYRTESGYADHRRPEQVLFVSDVKEDLRRRDFTINAMCRGLDGVVVDPYGGQEDS 142 S+EVTT+RTES Y HRRP QV F+ + DL RRD TINAM G + DP+ G D Sbjct: 83 SYEVTTFRTESEYEKHRRPLQVEFIDRLDGDLLRRDLTINAMAMNAAGELYDPFRGLNDL 142 Query: 143 ERRLIXXXXCVGDAEARFGEDALRMLRCVRFASVLDFSIAKNTWRGLLRQRDKLAHIAVE 202 + R I CVGDA+ARF EDALRMLR +RFAS +F+IA TWR L+R R+ L HIA+E Sbjct: 143 KNRTI---RCVGDADARFQEDALRMLRTIRFASQFEFTIAHTTWRALVRHRELLRHIAME 199 Query: 203 RVRAELERIVLGPHPQRGLGLVARSGLLPRGKAPFPWTGSDMAAA 247 RV AEL+R++ G HP R L L+A S LL +TG+ + AA Sbjct: 200 RVHAELDRMIGGAHPGRALHLLAASKLL-------RYTGTVLPAA 237 >B4BM25.1 B4BM25_9BACI Polynucleotide adenylyltransferase region Length = 404 Score = 229 bits (584), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 111/206 (53%), Positives = 149/206 (72%), Gaps = 3/206 (1%) Query: 24 QGYEAYFVGGCVRDELLGRPVHDMDLTTSAWPEEVMAIFPRCVPTGLAHGTITVLQDGWS 83 GYEAYFVGG VRD LLGRP+ D+D+ TSA P+EVMAIFP+ + G HGT+ V+ +G + Sbjct: 18 HGYEAYFVGGAVRDLLLGRPIGDVDIATSALPDEVMAIFPKTIDVGSKHGTVVVVHEGTA 77 Query: 84 FEVTTYRTESGYADHRRPEQVLFVSDVKEDLRRRDFTINAMCRGLDGVVVDPYGGQEDSE 143 +EVTT+RT+ Y DHRRPE V FV ++EDL+RRDFT+NA+ G ++DP+GGQE E Sbjct: 78 YEVTTFRTDGDYEDHRRPESVTFVRSLEEDLKRRDFTMNAIAMDEHGTIIDPFGGQEAIE 137 Query: 144 RRLIXXXXCVGDAEARFGEDALRMLRCVRFASVLDFSIAKNTWRGLLRQRDKLAHIAVER 203 +RLI VG+A+ARF EDALRM+R VRF S L FS+A +T + ++ LAHI+VER Sbjct: 138 KRLI---RTVGEADARFREDALRMMRAVRFVSQLGFSLAIDTKKAIIANAPLLAHISVER 194 Query: 204 VRAELERIVLGPHPQRGLGLVARSGL 229 + E+E+++ GP L L+A +GL Sbjct: 195 MTMEMEKLLAGPFAAEALPLLAETGL 220 >A4IQ63.1 CCA_GEOTN CCA-adding enzyme EC=2.7.7.25 EC=2.7.7.21 Length = 404 Score = 229 bits (584), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 111/206 (53%), Positives = 149/206 (72%), Gaps = 3/206 (1%) Query: 24 QGYEAYFVGGCVRDELLGRPVHDMDLTTSAWPEEVMAIFPRCVPTGLAHGTITVLQDGWS 83 GYEAYFVGG VRD LLGRP+ D+D+ TSA P+EVMAIFP+ + G HGT+ V+ +G + Sbjct: 18 HGYEAYFVGGAVRDLLLGRPIGDVDIATSALPDEVMAIFPKTIDVGSKHGTVVVVHEGTA 77 Query: 84 FEVTTYRTESGYADHRRPEQVLFVSDVKEDLRRRDFTINAMCRGLDGVVVDPYGGQEDSE 143 +EVTT+RT+ Y DHRRPE V FV ++EDL+RRDFT+NA+ G ++DP+GGQE E Sbjct: 78 YEVTTFRTDGDYEDHRRPESVTFVRSLEEDLKRRDFTMNAIAMDEHGTIIDPFGGQEAIE 137 Query: 144 RRLIXXXXCVGDAEARFGEDALRMLRCVRFASVLDFSIAKNTWRGLLRQRDKLAHIAVER 203 +RLI VG+A+ARF EDALRM+R VRF S L FS+A +T + ++ LAHI+VER Sbjct: 138 KRLI---RTVGEADARFREDALRMMRAVRFVSQLGFSLAIDTKKAIIANAPLLAHISVER 194 Query: 204 VRAELERIVLGPHPQRGLGLVARSGL 229 + E+E+++ GP L L+A +GL Sbjct: 195 MTMEMEKLLAGPFAAEALPLLAETGL 220 >C0XLT2.1 C0XLT2_LACHI tRNA nucleotidyltransferase/poly(A) polymerase EC=2.7.7.21 Length = 405 Score = 229 bits (583), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 106/205 (51%), Positives = 142/205 (69%), Gaps = 3/205 (1%) Query: 25 GYEAYFVGGCVRDELLGRPVHDMDLTTSAWPEEVMAIFPRCVPTGLAHGTITVLQDGWSF 84 GYEAYFVGG VRD +L RP+HD+D+ TSA+P EV ++F R TG+ HGT+ +L G + Sbjct: 29 GYEAYFVGGSVRDTILQRPIHDVDIATSAYPSEVKSLFKRTADTGIEHGTVMILDHGTGY 88 Query: 85 EVTTYRTESGYADHRRPEQVLFVSDVKEDLRRRDFTINAMCRGLDGVVVDPYGGQEDSER 144 EVTT+RTESGY D+RRP++V FV +KEDL+RRDFTINA+ +G + D +GG D + Sbjct: 89 EVTTFRTESGYQDYRRPDKVTFVRSLKEDLKRRDFTINALALSENGKITDLFGGLNDMSK 148 Query: 145 RLIXXXXCVGDAEARFGEDALRMLRCVRFASVLDFSIAKNTWRGLLRQRDKLAHIAVERV 204 ++I VGD E RF EDALRM+R VRFAS LDF I + T G+ + L IAVER+ Sbjct: 149 KII---RAVGDPEERFNEDALRMMRAVRFASQLDFKIEQQTLTGIYDHSELLKKIAVERI 205 Query: 205 RAELERIVLGPHPQRGLGLVARSGL 229 +E +++LG + GL L+ +S L Sbjct: 206 HSEFVKMMLGVDAKNGLNLMIKSQL 230 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.323 0.139 0.439 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,450,973,417 Number of extensions: 57348470 Number of successful extensions: 111732 Number of sequences better than 1.0e-05: 2138 Number of HSP's gapped: 108016 Number of HSP's successfully gapped: 2140 Length of query: 247 Length of database: 3,060,696,203 Length adjustment: 132 Effective length of query: 115 Effective length of database: 1,817,122,223 Effective search space: 208969055645 Effective search space used: 208969055645 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 129 (54.3 bits)