BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0256.0066.p (221 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KL80.1 B3KL80_9BACI Conserved hypothetical membrane protein 353 5e-96 B1DJ78.1 B1DJ78_9BACL Conserved hypothetical membrane protein 271 3e-71 B7GHM8.1 B7GHM8_ANOFW Membrane proteinase PrsW, regulator of ant... 213 9e-54 P50738.1 PRSW_BACSU Protease prsW EC=3.4.-.- 201 3e-50 Q65I04.1 PRSW_BACLD Protease prsW EC=3.4.-.- 200 9e-50 >B3KL80.1 B3KL80_9BACI Conserved hypothetical membrane protein Length = 231 Score = 353 bits (907), Expect = 5e-96, Method: Compositional matrix adjust. Identities = 160/220 (72%), Positives = 198/220 (90%) Query: 1 VAPGLALLTFFYLKDKYDQEPLHMVLKVFLLGLLIVFPIMIIQRGLVLGLDGGPYVDSFL 60 +APGLALLT+FYLKD+YDQEPLHMV+KVF+LG+LIVFP+MI+QRG++L L P V+S Sbjct: 11 LAPGLALLTYFYLKDRYDQEPLHMVIKVFMLGVLIVFPVMILQRGMILWLGDNPVVNSLF 70 Query: 61 ISSGVEECLKWFVLYHMIYNHTEFDEPYDGILYAVAISLGFATIENIMYAWYSHASIGSM 120 +S+GVEE LKWFV+YH+I+NHTEFDEPYDGILYAVAISLGFAT+EN++YAWYS A+ G M Sbjct: 71 VSAGVEEFLKWFVMYHIIFNHTEFDEPYDGILYAVAISLGFATVENLLYAWYSQAAFGPM 130 Query: 121 FMRALLPVSGHAMFGVIMGYHMGRAKFSGGVRTRGILFMSLLLPWLWHGIYDFILSTMTN 180 FMRALLPVSGHAMFGVIMGY+MG A+F+ G R R L +S++LPW+WHG+YD IL+ + + Sbjct: 131 FMRALLPVSGHAMFGVIMGYYMGHARFTEGKRRRFFLVLSMVLPWMWHGLYDLILNLLIH 190 Query: 181 TWIWFIVPLMAVLWYGGMGKVARANSRSPFRFLKREEEIN 220 TW+WFIVPLM +LWYGGMGK++RAN+RSPFRF+KREEE+N Sbjct: 191 TWVWFIVPLMVLLWYGGMGKISRANNRSPFRFIKREEEVN 230 >B1DJ78.1 B1DJ78_9BACL Conserved hypothetical membrane protein Length = 232 Score = 271 bits (692), Expect = 3e-71, Method: Compositional matrix adjust. Identities = 136/222 (61%), Positives = 177/222 (79%), Gaps = 1/222 (0%) Query: 1 VAPGLALLTFFYLKDKYDQEPLHMVLKVFLLGLLIVFPIMIIQRGLVLGL-DGGPYVDSF 59 VAPG++LLT+ YLKD+YD EP+HMV+++FLLG+LIV PI++IQRGL L D P + SF Sbjct: 11 VAPGISLLTYLYLKDRYDAEPIHMVVRMFLLGVLIVVPIVVIQRGLQLWFGDHQPMLFSF 70 Query: 60 LISSGVEECLKWFVLYHMIYNHTEFDEPYDGILYAVAISLGFATIENIMYAWYSHASIGS 119 + S+G+EE LKWFVLYHMIYNHTEFDEPYDGI+YA +ISLGFAT+EN+MYA YS ++ G+ Sbjct: 71 VESAGIEEFLKWFVLYHMIYNHTEFDEPYDGIVYAASISLGFATLENVMYAIYSPSTFGT 130 Query: 120 MFMRALLPVSGHAMFGVIMGYHMGRAKFSGGVRTRGILFMSLLLPWLWHGIYDFILSTMT 179 + MRALLPVSGHA+FGV+MGY++G+AKF+ + L +SL LP L HG YD+I+ + Sbjct: 131 LLMRALLPVSGHALFGVMMGYYVGKAKFATSSKRNIYLAISLCLPLLLHGTYDYIMISFK 190 Query: 180 NTWIWFIVPLMAVLWYGGMGKVARANSRSPFRFLKREEEINL 221 WI+ I+P M LW G+ K+ RANSRSPFRF+ RE+E+ L Sbjct: 191 TNWIYVIIPFMVALWVWGLRKMNRANSRSPFRFISREDEVKL 232 >B7GHM8.1 B7GHM8_ANOFW Membrane proteinase PrsW, regulator of anti-sigma factor RsiW Length = 224 Score = 213 bits (542), Expect = 9e-54, Method: Compositional matrix adjust. Identities = 107/206 (51%), Positives = 152/206 (73%), Gaps = 4/206 (1%) Query: 1 VAPGLALLTFFYLKDKYDQEPLHMVLKVFLLGLLIVFPIMIIQRGL-VLGLDGGPYVDSF 59 +APGLALL++FYLKD+Y+ EPL +VL++FL G +VFP+M IQ L V + P+V++F Sbjct: 9 IAPGLALLSYFYLKDEYETEPLSLVLRMFLYGAFLVFPLMFIQHVLKVEHMLTSPFVEAF 68 Query: 60 LISSGVEECLKWFVLYHMIYNHTEFDEPYDGILYAVAISLGFATIENIMYAWYSHASIGS 119 L +S +EE KWFV Y+ IY+H EF+EPYDGI+Y V++SLGFAT+ENI+Y + + Sbjct: 69 LSTSLLEEFFKWFVFYYAIYDHQEFNEPYDGIVYGVSVSLGFATLENILYLFANGVEFAV 128 Query: 120 MFMRALLPVSGHAMFGVIMGYHMGRAKFSGGVRTRGILFMSLLLPWLWHGIYDFILSTMT 179 RALLPVS HA+FGVIMG+++GRAKFS + + +++S LLP+L+HGIYD+IL T+ Sbjct: 129 --TRALLPVSSHALFGVIMGFYLGRAKFSLPKKEKNYIWLSFLLPFLFHGIYDYILLTLE 186 Query: 180 NTWIWFIVPLMAVLWYGGMGKVARAN 205 WI++++P M LW+ + KV A Sbjct: 187 R-WIYYMLPFMIFLWWLALRKVKLAR 211 >P50738.1 PRSW_BACSU Protease prsW EC=3.4.-.- Length = 218 Score = 201 bits (512), Expect = 3e-50, Method: Compositional matrix adjust. Identities = 106/207 (51%), Positives = 147/207 (71%), Gaps = 4/207 (1%) Query: 1 VAPGLALLTFFYLKDKYDQEPLHMVLKVFLLGLLIVFPIMIIQRGLVL-GLDGGPYVDSF 59 +APG+ALL++FYLKD+YD EP+HMVL+ F LG+++VFPIM IQ L + GG + SF Sbjct: 9 IAPGIALLSYFYLKDQYDNEPVHMVLRSFFLGVVLVFPIMFIQYVLEKENVGGGSFFVSF 68 Query: 60 LISSGVEECLKWFVLYHMIYNHTEFDEPYDGILYAVAISLGFATIENIMYAWYSHASIGS 119 L S +EE LKWF+L +Y H FDE YDGI+Y ++SLGFAT+ENI+Y H + Sbjct: 69 LSSGFLEESLKWFILMISVYPHAHFDEHYDGIVYGASVSLGFATLENILYL-IGHG-VEH 126 Query: 120 MFMRALLPVSGHAMFGVIMGYHMGRAKFSGGVRTRGILFMSLLLPWLWHGIYDFILSTMT 179 F+RALLPVS HA+ GVIMG+++G+A+FS L +SL++P L HG YDFIL+ ++ Sbjct: 127 AFVRALLPVSCHALIGVIMGFYLGKARFSADKARVKWLTLSLVVPSLLHGSYDFILTALS 186 Query: 180 NTWIWFIVPLMAVLWYGGMGKVARANS 206 N WI++++P M LW+ G+ K +A S Sbjct: 187 N-WIYYMLPFMVFLWWFGLRKAKKARS 212 >Q65I04.1 PRSW_BACLD Protease prsW EC=3.4.-.- Length = 222 Score = 200 bits (508), Expect = 9e-50, Method: Compositional matrix adjust. Identities = 105/207 (50%), Positives = 145/207 (70%), Gaps = 4/207 (1%) Query: 1 VAPGLALLTFFYLKDKYDQEPLHMVLKVFLLGLLIVFPIMIIQRGLVLG-LDGGPYVDSF 59 +APG+ALL +FYLK+KY+ EP+HMV+++F+LG+L+VFP M IQ L + G +V SF Sbjct: 9 LAPGIALLMYFYLKEKYESEPIHMVIRLFILGVLLVFPTMFIQYVLEKEHISEGSFVVSF 68 Query: 60 LISSGVEECLKWFVLYHMIYNHTEFDEPYDGILYAVAISLGFATIENIMYAWYSHASIGS 119 L S +EE LKWF+L + H +FDE YDGI+Y ++SLGFAT+ENI+Y + Sbjct: 69 LTSGFLEEFLKWFLLMVSTFQHIDFDEHYDGIVYGTSLSLGFATLENILYL--IGNGVEY 126 Query: 120 MFMRALLPVSGHAMFGVIMGYHMGRAKFSGGVRTRGILFMSLLLPWLWHGIYDFILSTMT 179 FMRALLPVS HA+FGVIMG+++G+A+FS L SL +P L HG+YD+IL + Sbjct: 127 AFMRALLPVSSHALFGVIMGFYIGKARFSDSKTKMKWLLCSLFIPALLHGLYDYILLALK 186 Query: 180 NTWIWFIVPLMAVLWYGGMGKVARANS 206 N WI+F++P MA LW+ G+ K +A S Sbjct: 187 N-WIYFMLPFMAFLWWFGLRKAKKARS 212 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.330 0.146 0.475 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,404,907,157 Number of extensions: 59530136 Number of successful extensions: 197650 Number of sequences better than 1.0e-05: 92 Number of HSP's gapped: 197481 Number of HSP's successfully gapped: 92 Length of query: 221 Length of database: 3,060,696,203 Length adjustment: 131 Effective length of query: 90 Effective length of database: 1,826,543,238 Effective search space: 164388891420 Effective search space used: 164388891420 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 128 (53.9 bits)