BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0256.0064.p (453 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KL82.1 B3KL82_9BACI Germination protein YpeB 608 e-172 B1DJ76.1 B1DJ76_9BACL Germination protein YpeB 514 e-144 C0ZCA7.1 C0ZCA7_BREBN Sporulation protein 376 e-102 B7GHM6.1 B7GHM6_ANOFW Sporulation protein YpeB 371 e-101 C2XV00.1 C2XV00_BACCE Putative uncharacterized protein 352 6e-95 >B3KL82.1 B3KL82_9BACI Germination protein YpeB Length = 449 Score = 608 bits (1567), Expect = e-172, Method: Compositional matrix adjust. Identities = 293/445 (65%), Positives = 357/445 (80%), Gaps = 3/445 (0%) Query: 1 MYKRLSALMFPITALLLVGALVWGYQENQEKNSILIKAENQYQRAFHDLSYHVEQLHGEL 60 MYKRLSA++FP+ +LL+GALVWGYQEN+EKN+ILI AENQYQRAFHDLSY+++++H EL Sbjct: 1 MYKRLSAILFPVATILLIGALVWGYQENKEKNAILINAENQYQRAFHDLSYNMDRIHAEL 60 Query: 61 GNTLAVNSTSSNMHRKGLVNVWRLTSEAQXXXXXXXXXXXXXFSKTEEFLSKISNFSYKA 120 GNTLAV+STS MHRKGL+NVWRLTSEAQ F+KTEEFLS+ISNF+YK Sbjct: 61 GNTLAVSSTSQGMHRKGLMNVWRLTSEAQNEISQLPLTLLP-FNKTEEFLSRISNFAYKT 119 Query: 121 AVRDFTKKPLTDNELGNLKALYKNSGEISKDLQDVQHKVITNRLRWMDVESALATEDKAE 180 VRD TK+PL++NE+ LK+LY NSGEI+KDLQ VQ+KVI N LRWMDVE+ALAT +K E Sbjct: 120 GVRDLTKEPLSENEVKTLKSLYANSGEITKDLQHVQNKVIANSLRWMDVETALAT-NKVE 178 Query: 181 DNTIIDGFKTVDKRVAAYPELDYGPSVASIYDKRSVKKLGGKPVTAEDVKRKALKFADLG 240 DN+IIDGFKTVDK+V AYPELD+GPSV+SIYD+RSVK+L G PV+ EDVKRKALKF+D G Sbjct: 179 DNSIIDGFKTVDKKVEAYPELDWGPSVSSIYDRRSVKQLAGMPVSEEDVKRKALKFSDTG 238 Query: 241 GNANVEIKENGKGTEWASYTATVTSQKHQQPISMDFTVEGGLLISYNDNRSVGPAQVSMK 300 ANV++ +NG GTEW SYTA V K +SMDFT +GG LISY D R+VG + S++ Sbjct: 239 NQANVQVTKNGSGTEWVSYTAKVKHPKGHM-LSMDFTEKGGQLISYTDERNVGAKRASVQ 297 Query: 301 QAVAKAGTFLQQKGYPNMTAVSADKYDNLGNLTFVTSRDGVLIYPEKITVRVGLDTGEAT 360 A+ KA FL +KGY +MT VSAD+YDNL N +FV DGVLIYPEKITVR +D GE Sbjct: 298 DAIDKAQQFLTKKGYSDMTVVSADQYDNLANFSFVREEDGVLIYPEKITVRSAMDNGEVI 357 Query: 361 GFQASDYVRKRQEKRQIPKPGITLAAARKMLHPEFKEMYNRLALIENGDAEEVLTYEFGG 420 GFQASD+V + Q KR IP+P ++LA A K+L+PEFK +Y+R ALI+N +E+VL YEFGG Sbjct: 358 GFQASDFVYEHQVKRDIPQPKLSLAEAEKVLNPEFKVLYHRKALIKNDRSEDVLCYEFGG 417 Query: 421 RINGSQYRIYLNAADGHEETVEEIR 445 RINGSQYRIY+NA G EETVE ++ Sbjct: 418 RINGSQYRIYINADTGLEETVEVVK 442 >B1DJ76.1 B1DJ76_9BACL Germination protein YpeB Length = 452 Score = 514 bits (1325), Expect = e-144, Method: Compositional matrix adjust. Identities = 259/455 (56%), Positives = 329/455 (72%), Gaps = 10/455 (2%) Query: 1 MYKRLSALMFPITALLLVGALVWGYQENQEKNSILIKAENQYQRAFHDLSYHVEQLHGEL 60 MY+RLSA++FPI L L+G++ WGYQE+QEKNSILIKAENQYQRAFHDL+YHVEQLH +L Sbjct: 1 MYRRLSAVLFPIMTLFLIGSVYWGYQEHQEKNSILIKAENQYQRAFHDLTYHVEQLHQQL 60 Query: 61 GNTLAVNSTSSNMHRKGLVNVWRLTSEAQXXXXXXXXXXXXXFSKTEEFLSKISNFSYKA 120 GNTLAVNSTS + HRKGLVNVWRLTS+AQ F++ E+FLS ISNF+YK Sbjct: 61 GNTLAVNSTSQSYHRKGLVNVWRLTSQAQNEINQLPLTILP-FNEAEDFLSHISNFAYKT 119 Query: 121 AVRDFTKKPLTDNELGNLKALYKNSGEISKDLQDVQHKVITNRLRWMDVESALATEDKAE 180 +VRD TK+PL+ +E LK LY S +ISKDL +Q KV+++ LRWMDVE ALA ++ Sbjct: 120 SVRDLTKQPLSQDEFKTLKTLYAKSEDISKDLYKMQEKVLSDHLRWMDVEVALAKQNSPN 179 Query: 181 DNTIIDGFKTVDKRVAAYPELDYGPSVASIYDKRSVKKLGGKPVTAEDVKRKALKFADLG 240 DNTIIDGFKTVDK+V+ YPE+++GPSVAS+Y KR++ L G VTAEDVK+ +F LG Sbjct: 180 DNTIIDGFKTVDKKVSEYPEINWGPSVASMYQKRTINMLSGSMVTAEDVKKHVTEF--LG 237 Query: 241 GN-ANVEIKENGKGTEWASYTATVTSQKHQQPISMDFTVEGGLLISYNDNRSVGPAQVSM 299 A + + ENGKGTE++S++ATVT K Q + MD+T GG LI + D R +G ++ Sbjct: 238 KKPAEIRVVENGKGTEYSSFSATVTEPKSGQKLQMDYTQRGGKLIWFMDTRDIGEKKIDQ 297 Query: 300 KQAVAKAGTFLQQKGYPNMTAVSADKYDNLGNLTFVTSRDGVLIYPEKITVRVGLDTGEA 359 +A A A FL + G+ M AVS + +DN G TFV++++GVLIYPEK+TV+V LD GEA Sbjct: 298 NKAQASAEKFLDEHGFKGMRAVSYNMFDNTGAFTFVSTQNGVLIYPEKLTVKVALDNGEA 357 Query: 360 TGFQASDYVRKRQEKRQIPKPGITLAAARKMLHPEFKEMYNRLALIENGDAEEVLTYEFG 419 G QA+DYV + Q KR IP ++ A ARK L+PEFK +++ALI N EE L YEF Sbjct: 358 VGLQANDYVYE-QHKRSIPAAKLSKAEARKALNPEFKVANDQMALIMNELGEEALCYEFT 416 Query: 420 GRINGSQYRIYLNAADGHEETVEEI-----RTSSG 449 G INGS YRIY+NA G EE +EE+ RTSSG Sbjct: 417 GSINGSDYRIYINAESGVEEAIEELSPLDKRTSSG 451 >C0ZCA7.1 C0ZCA7_BREBN Sporulation protein Length = 457 Score = 376 bits (966), Expect = e-102, Method: Compositional matrix adjust. Identities = 196/454 (43%), Positives = 285/454 (62%), Gaps = 8/454 (1%) Query: 1 MYKRLSALMFPITALLLVGALVWGYQENQEKNSILIKAENQYQRAFHDLSYHVEQLHGEL 60 +Y + ++FP+ + L+GA VWGYQE+ EKNS+LIKAEN YQRAFHDL+YHV++LH EL Sbjct: 2 VYGATARVLFPVALVALIGAGVWGYQEHNEKNSVLIKAENSYQRAFHDLTYHVDKLHDEL 61 Query: 61 GNTLAVNSTSSNMHRKGLVNVWRLTSEAQXXXXXXXXXXXXXFSKTEEFLSKISNFSYKA 120 G +L VNS L +VWRL+ AQ F+KTEE LS++++FSY+ Sbjct: 62 GKSLVVNS--RRQMTPILTSVWRLSYAAQADVGQLPLTLMA-FNKTEEMLSRVADFSYRV 118 Query: 121 AVRDFTKKPLTDNELGNLKALYKNSGEISKDLQDVQHKVITNRLRWMDVESALATEDKAE 180 AVRD K+PL+ +E LK+L+ + EI +L+ VQ + +LRWMDVE+ALA+ E Sbjct: 119 AVRDLDKQPLSQSEYKTLKSLHTQAKEIQDELRKVQTVAMDKQLRWMDVETALASNKNKE 178 Query: 181 DNTIIDGFKTVDKRVAAYPELDYGPSVASIYDKRS--VKKLGGKPVTAEDVKRKALKFAD 238 DN+IIDGF+T++K+V YP+LD+G + S+ K+ +K + GKP T E+ ++ AL F Sbjct: 179 DNSIIDGFQTIEKKVQEYPDLDWGVGIQSLDKKKQQRIKGIDGKPATKEEAEKSALSFLG 238 Query: 239 LG-GNANVEIKENGKGTEWASYTATVTSQKHQQPISMDFTVEGGLLISYNDNRSVGPAQV 297 L G+A VE+ +NGKG E+++Y+ VT H+ I++D T GG ++ + R +G ++ Sbjct: 239 LKPGDAKVEVNDNGKGQEYSAYSVHVTPNGHKTGINLDVTKRGGKVVWMMNERGIGEEKL 298 Query: 298 SMKQAVAKAGTFLQQKGYPNMTAVSADKYDNLGNLTFVTSRDGVLIYPEKITVRVGLDTG 357 MKQA K FLQ G+ NM D YD TFV +DGV IYP+ + V + LD G Sbjct: 299 DMKQAEEKGRVFLQNHGFDNMQVSEIDSYDRNALFTFVPVQDGVRIYPDGVVVEIALDNG 358 Query: 358 EATGFQASDYVRKRQEKRQIPKPGITLAAARKMLHPEFKEMYNRLALIE-NGDAEEVLTY 416 E T + +++Y+ + KR + KP ++ ARK ++P K RLALIE D +EVL + Sbjct: 359 EVTAYNSTEYLFNHK-KRILSKPRLSKEEARKKVNPSVKVTDQRLALIEGKNDGKEVLAW 417 Query: 417 EFGGRINGSQYRIYLNAADGHEETVEEIRTSSGA 450 E G G+ Y +Y+NA +G EE V ++ T SG+ Sbjct: 418 EMTGSFEGNSYMVYINALNGEEEEVVKMETGSGS 451 >B7GHM6.1 B7GHM6_ANOFW Sporulation protein YpeB Length = 448 Score = 371 bits (952), Expect = e-101, Method: Compositional matrix adjust. Identities = 197/440 (44%), Positives = 272/440 (61%), Gaps = 13/440 (2%) Query: 15 LLLVGAL--------VWGYQENQEKNSILIKAENQYQRAFHDLSYHVEQLHGELGNTLAV 66 +LL+GAL WGY+E+Q+KN+ILI AEN YQRAFHDL+Y ++ LH ++G TLA+ Sbjct: 4 ILLIGALSIGIAGTAYWGYREHQQKNAILIHAENNYQRAFHDLTYQIDLLHDQIGTTLAM 63 Query: 67 NSTSSNMHRKGLVNVWRLTSEAQXXXXXXXXXXXXXFSKTEEFLSKISNFSYKAAVRDFT 126 NS S L VWRL SEA F+KTEEFLS I +FSY+AA+RD Sbjct: 64 NSRQS--LSPALAEVWRLASEAHATVGQLPLSLLP-FNKTEEFLSNIGSFSYRAAIRDLD 120 Query: 127 KKPLTDNELGNLKALYKNSGEISKDLQDVQHKVITNRLRWMDVESALATEDKAEDNTIID 186 K+PL E L+ LYK S +I ++L++VQH V+ N LRWMDVE ALAT ++ DNTIID Sbjct: 121 KQPLNAEEYKALQQLYKKSADIQQELRNVQHLVLENNLRWMDVELALATNNRPNDNTIID 180 Query: 187 GFKTVDKRVAAYPELDYGPSVASIYDK-RSVKKLGGKPVTAEDVKRKALKFADLGGNANV 245 GFKTV+K V +Y E D+GPS S+ + + + + GK +T E+ K+ A F L GN + Sbjct: 181 GFKTVEKNVQSYNETDFGPSFTSVEKREKGFEHISGKKITEEEAKQIAKTFLGLKGNEKI 240 Query: 246 EIKENGKGTEWASYTATVTSQKHQQPISMDFTVEGGLLISYNDNRSVGPAQVSMKQAVAK 305 E+ +GKG A Y+ T+ + K + MD T GG I +NR V +S+ +A + Sbjct: 241 EVVHSGKGASDAFYSVTIKNPKTKSETYMDITERGGYPIWVIENRPVNKQNISLNEATMR 300 Query: 306 AGTFLQQKGYPNMTAVSADKYDNLGNLTFVTSRDGVLIYPEKITVRVGLDTGEATGFQAS 365 FL+Q + N + +YD++ LTFVT++DGV IYPE I +++ LD G GF A Sbjct: 301 GLQFLRQHKFNNFELYESTQYDHVAVLTFVTAKDGVRIYPESIKMKIALDDGNIIGFSAR 360 Query: 366 DYVRKRQEKRQIPKPGITLAAARKMLHPEFKEMYNRLALIENGDAEEVLTYEFGGRINGS 425 DY+ ++E R IPKP I++ ARK ++P M R A+I N EEVL YEF G + Sbjct: 361 DYLSSKRE-RTIPKPAISIEQARKKMNPNVTIMEERKAMIMNNMNEEVLCYEFLGTLGDD 419 Query: 426 QYRIYLNAADGHEETVEEIR 445 YRI++NA +G EE VE+++ Sbjct: 420 TYRIFINAQNGVEEKVEKLQ 439 >C2XV00.1 C2XV00_BACCE Putative uncharacterized protein Length = 446 Score = 352 bits (902), Expect = 6e-95, Method: Compositional matrix adjust. Identities = 188/442 (42%), Positives = 270/442 (61%), Gaps = 5/442 (1%) Query: 5 LSALMFPITALLLVGALVWGYQENQEKNSILIKAENQYQRAFHDLSYHVEQLHGELGNTL 64 L ++ + + +VG WGY+E+QEKN++LI+AEN YQRAFHDL+Y V+ LH ++G TL Sbjct: 2 LRGIIIVLLTVGVVGTGYWGYKEHQEKNAVLIRAENSYQRAFHDLAYEVDLLHDKIGTTL 61 Query: 65 AVNSTSSNMHRKGLVNVWRLTSEAQXXXXXXXXXXXXXFSKTEEFLSKISNFSYKAAVRD 124 A+NS SS LV+VWRLTSEA+ F+KTEEFL+ I +FSY+AAVRD Sbjct: 62 AMNSRSS--LSPALVDVWRLTSEARSDVGQLPLTLMP-FNKTEEFLANIGDFSYRAAVRD 118 Query: 125 FTKKPLTDNELGNLKALYKNSGEISKDLQDVQHKVITNRLRWMDVESALATEDKAEDNTI 184 K PL D E L+ LY N+G I +L+ VQH V+ N LRWMDVE ALA+ DNTI Sbjct: 119 LEKDPLNDQEYKTLQGLYSNAGNIQDELRKVQHLVLKNNLRWMDVEMALASNQDPADNTI 178 Query: 185 IDGFKTVDKRVAAYPELDYGPSVASIY-DKRSVKKLGGKPVTAEDVKRKALKFADLGGNA 243 IDG KTV+K V +Y ++GP+ S +K+ + GK ++ E+ ++ A F +L GN Sbjct: 179 IDGLKTVEKNVTSYSSTNFGPTFTSAQKNKKGGFEAKGKAISKEEAEKIAKSFLNLKGNE 238 Query: 244 NVEIKENGKGTEWASYTATVTSQKHQQPISMDFTVEGGLLISYNDNRSVGPAQVSMKQAV 303 VE++++GKG + + Y+ + MD T +GG I +NR + ++S+ A Sbjct: 239 KVEVEKSGKGAKESFYSVKIQDPATNNEYYMDITGKGGYPIWVMNNREIKEQKISLNDAG 298 Query: 304 AKAGTFLQQKGYPNMTAVSADKYDNLGNLTFVTSRDGVLIYPEKITVRVGLDTGEATGFQ 363 +K TFL+ + NM + +YDN+G T+V S +GV IYPE I +++ LD G GF Sbjct: 299 SKGLTFLKDHKFTNMELFDSSQYDNIGVFTYVVSENGVRIYPEAIQMKIALDDGSIVGFS 358 Query: 364 ASDYVRKRQEKRQIPKPGITLAAARKMLHPEFKEMYNRLALIENGDAEEVLTYEFGGRIN 423 A +Y+ Q KR IP +T A ARK ++P+ K M R A++ N EVL YEF G + Sbjct: 359 AKEYLASHQ-KRTIPSAKLTAAEARKKINPDVKVMEERKAVVVNDLHNEVLCYEFVGTLG 417 Query: 424 GSQYRIYLNAADGHEETVEEIR 445 Y+I++NA G EE V++++ Sbjct: 418 KDTYQIFINANSGAEEKVKKMQ 439 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.313 0.130 0.364 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,524,120,312 Number of extensions: 102570540 Number of successful extensions: 216727 Number of sequences better than 1.0e-05: 148 Number of HSP's gapped: 216229 Number of HSP's successfully gapped: 148 Length of query: 453 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 314 Effective length of database: 1,751,175,118 Effective search space: 549868987052 Effective search space used: 549868987052 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 133 (55.8 bits)