BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0256.0063.p (222 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KL84.1 B3KL84_9BACI Type IV pilus assembly PilZ 277 5e-73 B1DJ75.1 B1DJ75_9BACL Type IV pilus assembly PilZ 239 9e-62 C0ZCA8.1 C0ZCA8_BREBN Putative uncharacterized protein 125 4e-27 A8VXS5.1 A8VXS5_9BACI Asparaginase/glutaminase 103 7e-21 Q2B633.1 Q2B633_9BACI Putative uncharacterized protein 102 3e-20 >B3KL84.1 B3KL84_9BACI Type IV pilus assembly PilZ Length = 217 Score = 277 bits (708), Expect = 5e-73, Method: Compositional matrix adjust. Identities = 129/217 (59%), Positives = 162/217 (74%) Query: 6 LYPKINEYLYIQXXXXXXXXXXXXYRSRIAEMEEEAFLIEIPMQEKNGRLKKLFIGDELS 65 +YPKIN+ LYIQ Y+SRI++M++E+ +E+P+QE GRLKKL+IGDELS Sbjct: 1 MYPKINDVLYIQLDTGDEKEASIVYKSRISDMDDESMYMEVPLQEGTGRLKKLYIGDELS 60 Query: 66 VYFLTEGGIKNYFNTHVIGFKEDVIRMVKIRKPAADSIFKIQRRSFLRVNAELELAVKDS 125 YFLTEGG+KN+FN++V+ EDVIR+V+I+KP D I K+QRRSFLRV AELELAV Sbjct: 61 AYFLTEGGVKNFFNSYVLSHHEDVIRLVRIKKPEPDQITKVQRRSFLRVQAELELAVMAK 120 Query: 126 LGGRFLVHTEDIGGGGTSFLSDAKTSMKIGDGLSCWVLINYRNGSTEHANFEGEIVRIKK 185 G R L T+D+GGGG SF + S G LSCW+LI ++NGS EH FEGE+VR+K+ Sbjct: 121 DGTRLLTKTDDVGGGGVSFYLEPDKSFAEGSLLSCWILIPFKNGSVEHVPFEGEVVRVKQ 180 Query: 186 LENGRSLVMLKFAEISDSERQKIIRYCFERQFDFRNR 222 L RS+VMLKF +I D ERQK+IRYCFERQFDFRNR Sbjct: 181 LPGNRSIVMLKFNQIVDMERQKLIRYCFERQFDFRNR 217 >B1DJ75.1 B1DJ75_9BACL Type IV pilus assembly PilZ Length = 217 Score = 239 bits (611), Expect = 9e-62, Method: Compositional matrix adjust. Identities = 110/217 (50%), Positives = 158/217 (72%) Query: 6 LYPKINEYLYIQXXXXXXXXXXXXYRSRIAEMEEEAFLIEIPMQEKNGRLKKLFIGDELS 65 + P++N+ L+ + Y++RIA+ E+ LIE P+ +GR+K+L++GDELS Sbjct: 1 MLPRVNQMLFFEIASADEEEAAVEYKARIADELEDGLLIENPLNITSGRMKRLYLGDELS 60 Query: 66 VYFLTEGGIKNYFNTHVIGFKEDVIRMVKIRKPAADSIFKIQRRSFLRVNAELELAVKDS 125 VY++++ GIK+YF++HVIGF++DV++++KIRKP + I KIQRR FLRV+A+LE+AV S Sbjct: 61 VYYVSDDGIKHYFDSHVIGFRDDVVKLIKIRKPDPERITKIQRRHFLRVSADLEIAVNLS 120 Query: 126 LGGRFLVHTEDIGGGGTSFLSDAKTSMKIGDGLSCWVLINYRNGSTEHANFEGEIVRIKK 185 RF+ T+D+GGGG SF+ D K S+ G + CW+LI Y+NG+ EHA+F+ E+VR+ Sbjct: 121 GNIRFVTVTDDVGGGGISFICDGKWSIASGMEMDCWLLIPYKNGTLEHASFKAEVVRVVD 180 Query: 186 LENGRSLVMLKFAEISDSERQKIIRYCFERQFDFRNR 222 L GR VM KF ISDSERQKIIR+CFERQ +FR R Sbjct: 181 LVTGRKQVMCKFNAISDSERQKIIRFCFERQLEFRGR 217 >C0ZCA8.1 C0ZCA8_BREBN Putative uncharacterized protein Length = 227 Score = 125 bits (313), Expect = 4e-27, Method: Compositional matrix adjust. Identities = 68/222 (30%), Positives = 118/222 (53%), Gaps = 5/222 (2%) Query: 6 LYPKINEYLYIQXXXXXXXXXXXXYRSRIAEMEEEAFLIEIPMQEKNGRLKKLFIGDELS 65 + P+I + + + ++SR+A+++E+ IE+P E GR G E Sbjct: 2 MLPRIGQIIRLNFTSSSEETELQTFKSRVADLKEDVASIELPTSENTGRTGVFQPGTECL 61 Query: 66 VYFLTEGGIKNYFNTHVIGFKEDVIRMVKIRKPAADSIFKIQRRSFLRVNAELELAVKDS 125 V+++ E G + F + ++G + + I ++ ++ PA + I + QRR++LR++ +++AVK Sbjct: 62 VWYVGEDGSRYEFRSAIVGRQNNHIPVLLLQLPAKEEIVRTQRRNYLRIDTTVDIAVKLD 121 Query: 126 LGGR---FLVHTEDIGGGGTSFLSDAKTSMKIGDGLSCWVLINYRNGSTEHANFEGEIVR 182 R FL T DI GGG SF + +K D L W+ + +NG +HA EIVR Sbjct: 122 DPIRRYHFLARTLDISGGGLSFSCEESYHLKEKDNLHVWISLPSKNGQVQHAFVVMEIVR 181 Query: 183 IKKLENG--RSLVMLKFAEISDSERQKIIRYCFERQFDFRNR 222 K E + KF +IS+ +R K++R C+ERQ + R + Sbjct: 182 QKPAEEKGLHQWISGKFTQISEQDRAKVVRACYERQLELRKK 223 >A8VXS5.1 A8VXS5_9BACI Asparaginase/glutaminase Length = 220 Score = 103 bits (258), Expect = 7e-21, Method: Compositional matrix adjust. Identities = 61/197 (30%), Positives = 105/197 (53%), Gaps = 8/197 (4%) Query: 30 YRSRIAEMEEEAFLIEIPMQEKNGRLKKLFIGDELSVYFLTEGGIKNYFNTHVIGFKEDV 89 ++S++ + E+ I+ P+ E+N + G E V+F + F T V+G + Sbjct: 23 FKSKVLDYEKTFVYIDFPVNEENKKPSYFMEGTEFRVWFAGKDQAVYSFETQVLGKVDRG 82 Query: 90 IRMVKIRKPAADSIFKIQRRSFLRVNAELELAVKDSLGG--RFLVHTEDIGGGGTSFLSD 147 I M+ ++ P D +IQRR ++RV+ +++AV G F T DI GGG + + Sbjct: 83 IPMLVLKDPGKDEYLRIQRREYVRVDTAVDVAVHPKREGLSPFTTVTLDISGGGAALVLQ 142 Query: 148 AKTSMKIGDGLSCWVLINYRNGSTEHANFEGEIVRIKKLENGRSLV--MLKFAEISDSER 205 +K D L W+ I +R G E+ E++RI +G++ + KF++I++ +R Sbjct: 143 GNHKLKEKDELDLWISIPFRVGGIEYVQATAEVIRII---DGKTPIKCSCKFSDINEHDR 199 Query: 206 QKIIRYCFERQFDFRNR 222 QK+IRY FE+Q + RNR Sbjct: 200 QKVIRYAFEKQLE-RNR 215 >Q2B633.1 Q2B633_9BACI Putative uncharacterized protein Length = 219 Score = 102 bits (253), Expect = 3e-20, Method: Compositional matrix adjust. Identities = 57/196 (29%), Positives = 103/196 (52%), Gaps = 4/196 (2%) Query: 30 YRSRIAEMEEEAFLIEIPMQEKNGRLKKLFIGDELSVYFLTEGGIKNYFNTHVIGFKEDV 89 YR ++AE + A I+ P+ K+G+ L G +L F+++ G F T V G + Sbjct: 21 YRCKLAEKNDNALFIDYPVNVKSGKTAFLLDGTQLKATFVSDDGSVFLFTTEVKGRVKQN 80 Query: 90 IRMVKIRKPAADSIFKIQRRSFLRVNAELELAVKDSLG---GRFLVHTEDIGGGGTSFLS 146 I ++ + P + KIQRR ++R+ A ++ AV+ +G F TEDI GG S L Sbjct: 81 IPLMVLSYPGPKQLIKIQRRQYVRIEAAIDAAVQ-PIGREFSPFKAVTEDISAGGASLLV 139 Query: 147 DAKTSMKIGDGLSCWVLINYRNGSTEHANFEGEIVRIKKLENGRSLVMLKFAEISDSERQ 206 D + + G + +++ +NG + + +IVRI + ++ ++FA++ S RQ Sbjct: 140 DKDSPLDAGIFIKVLLVLALQNGDYHYLKADAKIVRIIEHNELKNKASIEFADLGPSGRQ 199 Query: 207 KIIRYCFERQFDFRNR 222 ++R+CF+RQ + + Sbjct: 200 LLLRFCFDRQLALKKK 215 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.141 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,211,269,134 Number of extensions: 49674776 Number of successful extensions: 95020 Number of sequences better than 1.0e-05: 34 Number of HSP's gapped: 95004 Number of HSP's successfully gapped: 34 Length of query: 222 Length of database: 3,060,696,203 Length adjustment: 131 Effective length of query: 91 Effective length of database: 1,826,543,238 Effective search space: 166215434658 Effective search space used: 166215434658 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 128 (53.9 bits)