BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0256.0061.p (157 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KL87.1 B3KL87_9BACI Phospholipid/glycerol acyltransferase 182 8e-45 B1DJ72.1 B1DJ72_9BACL 1-acyl-sn-glycerol-3-phosphate acyltransfe... 146 7e-34 Q5WGU7.1 Q5WGU7_BACSK 1-acylglycerol-3-phosphate O-acyltransfera... 132 1e-29 C0ZCB0.1 C0ZCB0_BREBN 1-acylglycerol-3-phosphate O-acyltransfera... 132 1e-29 C0GDV1.1 C0GDV1_9FIRM 1-acyl-sn-glycerol-3-phosphate acyltransfe... 132 1e-29 >B3KL87.1 B3KL87_9BACI Phospholipid/glycerol acyltransferase Length = 196 Score = 182 bits (462), Expect = 8e-45, Method: Compositional matrix adjust. Identities = 84/140 (60%), Positives = 106/140 (75%) Query: 1 MIYVICRGLLRFIYAILFPLKIIGKENVPEEGGVLLCANHISLLDPMTIGIKLRRQVKYM 60 MIYV CR ++R ++A L+ + +G N+P EGGVL+C+NHIS+ DP+++GI ++RQVK+M Sbjct: 1 MIYVFCRAVVRGLFAFLYRFESVGVHNIPSEGGVLICSNHISVRDPISVGIHVKRQVKFM 60 Query: 61 AKAELFKVPVLGWLIDKLGAFPVKRGGVSKESIKTALNTLRSGHVMGIFPEGTRXXXXXX 120 AKAELF +PV GWL+ +LGAFPVKRGGVSK+SIKTAL LRSG +MGIFPEGTR Sbjct: 61 AKAELFNIPVFGWLVRQLGAFPVKRGGVSKDSIKTALTILRSGEIMGIFPEGTRNSDATA 120 Query: 121 XXXXXXXXXLRSGAAVVPAA 140 LRSGA VVPAA Sbjct: 121 AKRGAASFALRSGATVVPAA 140 >B1DJ72.1 B1DJ72_9BACL 1-acyl-sn-glycerol-3-phosphate acyltransferase Length = 191 Score = 146 bits (368), Expect = 7e-34, Method: Compositional matrix adjust. Identities = 75/140 (53%), Positives = 96/140 (68%), Gaps = 1/140 (0%) Query: 1 MIYVICRGLLRFIYAILFPLKIIGKENVPEEGGVLLCANHISLLDPMTIGIKLRRQVKYM 60 M+Y I + + +Y +LF L++ G EN+P+EG V+LC+NHISLLDP +GI++ R V YM Sbjct: 1 MLYGIFKAVFNVLYGLLFRLEVRGLENIPKEGSVILCSNHISLLDPPAVGIRVPRMVHYM 60 Query: 61 AKAELFKVPVLGWLIDKLGAFPVKRGGVSKESIKTALNTLRSGHVMGIFPEGTRXXXXXX 120 AKAELFK+PV G LI GAFPVKR GV K++I++ALN L G VMGIFPEGTR Sbjct: 61 AKAELFKIPVFGPLIRAWGAFPVKR-GVGKDAIRSALNLLGEGKVMGIFPEGTRNSDGAA 119 Query: 121 XXXXXXXXXLRSGAAVVPAA 140 RSGAA++P A Sbjct: 120 AKKGAAMIAYRSGAAIIPVA 139 >Q5WGU7.1 Q5WGU7_BACSK 1-acylglycerol-3-phosphate O-acyltransferase EC=2.3.1.51 Length = 196 Score = 132 bits (332), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 70/141 (49%), Positives = 91/141 (64%), Gaps = 2/141 (1%) Query: 2 IYVICRGLLRFIYAILFPLKIIGKENVPEEGGVLLCANHISLLDPMTIGIKLRRQVKYMA 61 +Y + + R LF ++I GKEN+P+EG VLLCANHIS DP +G ++R V+YMA Sbjct: 3 LYQFGQAVCRLYLNALFKVRIYGKENIPDEGAVLLCANHISNNDPPLLGAYIKRPVRYMA 62 Query: 62 KAELFKVPVLGWLIDKLGAFPVKRGGVSKESIKTALNTLRSGHVMGIFPEGTRXXXXXXX 121 K ELFKVPVL L+ KLGAFPV R G K+S++T+L+ L+SG ++GIFPEGTR Sbjct: 63 KKELFKVPVLKTLLRKLGAFPVNRAGGDKQSLRTSLDILKSGDMVGIFPEGTRSKTGEIG 122 Query: 122 XXXXXX--XXLRSGAAVVPAA 140 L+S A VVP A Sbjct: 123 KGLTGAGFFALKSTAHVVPCA 143 >C0ZCB0.1 C0ZCB0_BREBN 1-acylglycerol-3-phosphate O-acyltransferase EC=2.3.1.51 Length = 197 Score = 132 bits (332), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 67/140 (47%), Positives = 90/140 (64%), Gaps = 2/140 (1%) Query: 3 YVICRGLLRFIYAILFPLKIIGKENVPEEGGVLLCANHISLLDPMTIGIKLRRQVKYMAK 62 Y + RG R I++++F ++IG+E++P+EG V+LCANHISL DP +G + RQV +MAK Sbjct: 4 YRLFRGFFRIIFSLVFRWQVIGREHIPKEGPVILCANHISLWDPPLLGGGIERQVLFMAK 63 Query: 63 AELFKVPVLGWLIDKLGAFPVKRGGVSKESIKTALNTLRSGHVMGIFPEGTRXXXXX--X 120 ELFK+PVL +LI K GAFPVKRG + +I+T L L G + GIFPEGTR Sbjct: 64 EELFKIPVLSFLITKFGAFPVKRGAGDRAAIRTTLKLLEDGKIFGIFPEGTRSKTGEPGE 123 Query: 121 XXXXXXXXXLRSGAAVVPAA 140 L+S A V+P A Sbjct: 124 AMPGVAMFALKSEAVVIPVA 143 >C0GDV1.1 C0GDV1_9FIRM 1-acyl-sn-glycerol-3-phosphate acyltransferase Length = 193 Score = 132 bits (331), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 67/142 (47%), Positives = 90/142 (63%), Gaps = 2/142 (1%) Query: 1 MIYVICRGLLRFIYAILFPLKIIGKENVPEEGGVLLCANHISLLDPMTIGIKLRRQVKYM 60 M+Y + R L +I+ I + + G+EN+P+EG LLCANHIS DP +G R V++M Sbjct: 1 MLYKVIRQLFAWIFQIFYRWDVQGRENLPDEGAFLLCANHISWWDPPLMGAVTSRTVRFM 60 Query: 61 AKAELFKVPVLGWLIDKLGAFPVKRGGVSKESIKTALNTLRSGHVMGIFPEGTRXXXXXX 120 AK ELFK+PV G ++ K+ AFPVKR + +IKTAL+TL+SG V+GIFPEGTR Sbjct: 61 AKEELFKIPVFGKILPKVNAFPVKRDSADRRAIKTALDTLKSGGVVGIFPEGTRSKTDDL 120 Query: 121 XXXXXXX--XXLRSGAAVVPAA 140 ++S A VVPAA Sbjct: 121 LPPQAGVGLIAVKSEAPVVPAA 142 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.326 0.145 0.432 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 731,557,269 Number of extensions: 26328467 Number of successful extensions: 66163 Number of sequences better than 1.0e-05: 1252 Number of HSP's gapped: 66025 Number of HSP's successfully gapped: 1252 Length of query: 157 Length of database: 3,060,696,203 Length adjustment: 118 Effective length of query: 39 Effective length of database: 1,949,016,433 Effective search space: 76011640887 Effective search space used: 76011640887 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 125 (52.8 bits)