BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0256.0058.p (375 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KL89.1 B3KL89_9BACI Small GTP-binding protein 598 e-169 B1DJ67.1 B1DJ67_9BACL Small GTP-binding protein 582 e-164 C4L6M3.1 C4L6M3_EXISA Small GTP-binding protein 481 e-134 B7GHN9.1 B7GHN9_ANOFW Predicted GTPase 480 e-133 B4BL07.1 B4BL07_9BACI Small GTP-binding protein 471 e-131 >B3KL89.1 B3KL89_9BACI Small GTP-binding protein Length = 440 Score = 598 bits (1541), Expect = e-169, Method: Compositional matrix adjust. Identities = 295/355 (83%), Positives = 326/355 (91%) Query: 1 LAIVEDKPGITRDRIYGVSDWNGKSFSVIDTGGIEIDGEDAILKSIRVQAELAIEEADVI 60 LAIVEDKPGITRDRIYG ++WNGK+FSVIDTGGIEIDG+D ILKSIR+QAELAIEEADVI Sbjct: 28 LAIVEDKPGITRDRIYGSAEWNGKAFSVIDTGGIEIDGDDMILKSIRMQAELAIEEADVI 87 Query: 61 VFMCEAKSGLTNADEEVAQILFRSGKPVVLAINKVDNMKRTEDIYEFYSLGIGDPXXXXX 120 VFMC+AK+G+T +DEEVAQILFRSGKPV+L++NKVDN+KR +DIYEFYSLG GDP Sbjct: 88 VFMCDAKTGVTQSDEEVAQILFRSGKPVILSVNKVDNLKRADDIYEFYSLGFGDPVGISG 147 Query: 121 XXXXXXXDLLDAVTERLPEPVEEEYDDDIIRVALIGRPNVGKSSLVNAILGEERVIVSDV 180 DLLD V E LPE ++EYD+D+IRVALIGRPNVGKSSLVNAILGEERVIVSDV Sbjct: 148 SHGTGIGDLLDVVVENLPELEDDEYDEDVIRVALIGRPNVGKSSLVNAILGEERVIVSDV 207 Query: 181 AGTTRDAIDTPFERDGQRYVLIDTAGMRKRGKVYETTEKYSVMRAMRAIERADVVLVVIN 240 AGTTRDAIDTPFE+DGQRYVLIDTAGMRKRGKVYETTEKYSVMRAMRAIERADVVLV+IN Sbjct: 208 AGTTRDAIDTPFEKDGQRYVLIDTAGMRKRGKVYETTEKYSVMRAMRAIERADVVLVLIN 267 Query: 241 GEEGIIDQDKHIAGYAHDAGKASIFVVNKWDAVEKDDKTMQNFENTIRDHFLFMSYAPVV 300 GEEGII+QDKHIAGYA++AGKAS+FVVNKWD VEKDDKTMQ FE IRDHFLFM+YAPVV Sbjct: 268 GEEGIIEQDKHIAGYAYEAGKASLFVVNKWDMVEKDDKTMQQFEKKIRDHFLFMTYAPVV 327 Query: 301 FLSALTKQRLQKLLPVVQHVAQQHALRITTHLVNDVVSDAVAINPPPTDKGRRLR 355 FLSA TKQRLQKLLPVV+HVA QH+LR+ THL+NDV+SDA+AINPPPTDKGRRLR Sbjct: 328 FLSAKTKQRLQKLLPVVKHVADQHSLRVQTHLLNDVISDAIAINPPPTDKGRRLR 382 Score = 79.3 bits (194), Expect = 5e-13, Method: Compositional matrix adjust. Identities = 47/126 (37%), Positives = 73/126 (57%), Gaps = 2/126 (1%) Query: 152 VALIGRPNVGKSSLVNAILGEERVIVSDVAGTTRDAIDTPFERDGQRYVLIDTAGMRKRG 211 VA++GRPNVGKS++ N ++G+ IV D G TRD I E +G+ + +IDT G+ G Sbjct: 6 VAIVGRPNVGKSTIFNRLIGDRLAIVEDKPGITRDRIYGSAEWNGKAFSVIDTGGIEIDG 65 Query: 212 KVYETTEKYSVMRAMRAIERADVVLVVINGEEGIIDQDKHIAGYAHDAGKASIFVVNKWD 271 + K M+A AIE ADV++ + + + G+ D+ +A +GK I VNK D Sbjct: 66 D--DMILKSIRMQAELAIEEADVIVFMCDAKTGVTQSDEEVAQILFRSGKPVILSVNKVD 123 Query: 272 AVEKDD 277 +++ D Sbjct: 124 NLKRAD 129 >B1DJ67.1 B1DJ67_9BACL Small GTP-binding protein Length = 440 Score = 582 bits (1501), Expect = e-164, Method: Compositional matrix adjust. Identities = 275/355 (77%), Positives = 318/355 (89%) Query: 1 LAIVEDKPGITRDRIYGVSDWNGKSFSVIDTGGIEIDGEDAILKSIRVQAELAIEEADVI 60 LAIVEDKPG+TRDR+Y +WNGK+FS++DTGGIEIDGED I+KS+R+QAELAIEEADVI Sbjct: 28 LAIVEDKPGVTRDRLYSPGEWNGKAFSIVDTGGIEIDGEDEIMKSVRMQAELAIEEADVI 87 Query: 61 VFMCEAKSGLTNADEEVAQILFRSGKPVVLAINKVDNMKRTEDIYEFYSLGIGDPXXXXX 120 +FMC+AK+G+T+AD+EVAQ+LFRS KPVVLA+NKVDN+ R ++IYEFY LG G P Sbjct: 88 IFMCDAKTGVTHADDEVAQMLFRSHKPVVLAVNKVDNLNRMDEIYEFYGLGFGTPIAVSG 147 Query: 121 XXXXXXXDLLDAVTERLPEPVEEEYDDDIIRVALIGRPNVGKSSLVNAILGEERVIVSDV 180 D+LDA E+LPE ++ YDDD+IRVALIGRPNVGKSSLVNA+LGEERVIVS+V Sbjct: 148 SHGLGIGDMLDAAIEKLPELEDDGYDDDVIRVALIGRPNVGKSSLVNALLGEERVIVSNV 207 Query: 181 AGTTRDAIDTPFERDGQRYVLIDTAGMRKRGKVYETTEKYSVMRAMRAIERADVVLVVIN 240 AGTTRDAIDTPFERDGQ+YVLIDTAGMRKRGKVYE+TEKYSVMRA++AIERADVVLV+IN Sbjct: 208 AGTTRDAIDTPFERDGQKYVLIDTAGMRKRGKVYESTEKYSVMRALKAIERADVVLVLIN 267 Query: 241 GEEGIIDQDKHIAGYAHDAGKASIFVVNKWDAVEKDDKTMQNFENTIRDHFLFMSYAPVV 300 GEEGII+QDKHIAGYAH+AGKASIFVVNKWD V+KDDKTMQ F IRDHFLFM+YAP+V Sbjct: 268 GEEGIIEQDKHIAGYAHEAGKASIFVVNKWDVVDKDDKTMQEFTQNIRDHFLFMTYAPIV 327 Query: 301 FLSALTKQRLQKLLPVVQHVAQQHALRITTHLVNDVVSDAVAINPPPTDKGRRLR 355 FLSA TKQRL KLLPVV HV++QHA+RI THL+NDVVSDAVA NPPPTDKG+RL+ Sbjct: 328 FLSAKTKQRLHKLLPVVNHVSEQHAMRIQTHLLNDVVSDAVAYNPPPTDKGKRLK 382 Score = 79.3 bits (194), Expect = 5e-13, Method: Compositional matrix adjust. Identities = 43/132 (32%), Positives = 76/132 (57%), Gaps = 2/132 (1%) Query: 152 VALIGRPNVGKSSLVNAILGEERVIVSDVAGTTRDAIDTPFERDGQRYVLIDTAGMRKRG 211 +A++GRPNVGKS++ N ++G+ IV D G TRD + +P E +G+ + ++DT G+ G Sbjct: 6 IAIVGRPNVGKSTIFNRVVGDRLAIVEDKPGVTRDRLYSPGEWNGKAFSIVDTGGIEIDG 65 Query: 212 KVYETTEKYSVMRAMRAIERADVVLVVINGEEGIIDQDKHIAGYAHDAGKASIFVVNKWD 271 + + K M+A AIE ADV++ + + + G+ D +A + K + VNK D Sbjct: 66 E--DEIMKSVRMQAELAIEEADVIIFMCDAKTGVTHADDEVAQMLFRSHKPVVLAVNKVD 123 Query: 272 AVEKDDKTMQNF 283 + + D+ + + Sbjct: 124 NLNRMDEIYEFY 135 >C4L6M3.1 C4L6M3_EXISA Small GTP-binding protein Length = 436 Score = 481 bits (1239), Expect = e-134, Method: Compositional matrix adjust. Identities = 233/355 (65%), Positives = 288/355 (81%), Gaps = 1/355 (0%) Query: 1 LAIVEDKPGITRDRIYGVSDWNGKSFSVIDTGGIEIDGEDAILKSIRVQAELAIEEADVI 60 ++IV+DKPG+TRDRIYG +W + F +IDTGGIE+ G++ +L +R QAELAI+EADVI Sbjct: 28 VSIVDDKPGVTRDRIYGTGEWLNRKFHLIDTGGIEV-GDEPLLVQMRHQAELAIDEADVI 86 Query: 61 VFMCEAKSGLTNADEEVAQILFRSGKPVVLAINKVDNMKRTEDIYEFYSLGIGDPXXXXX 120 +FM + G+T ADEEVA +LFRS KP+VLA+NKVDN + + +YEFYSLG GDP Sbjct: 87 IFMVNGREGITAADEEVANLLFRSNKPIVLAVNKVDNFEMRDLMYEFYSLGFGDPFPISG 146 Query: 121 XXXXXXXDLLDAVTERLPEPVEEEYDDDIIRVALIGRPNVGKSSLVNAILGEERVIVSDV 180 DLLD V E PE E EY++D+I+ +LIGRPNVGKSSL N+ILGEERVIVS++ Sbjct: 147 THGLGLGDLLDQVFELAPEKDEYEYNEDVIQFSLIGRPNVGKSSLTNSILGEERVIVSNI 206 Query: 181 AGTTRDAIDTPFERDGQRYVLIDTAGMRKRGKVYETTEKYSVMRAMRAIERADVVLVVIN 240 AGTTRDAIDTPF RDGQ YV+IDTAGMRKRGKVYE+TE+YSVMRA +AIER++VVLVV++ Sbjct: 207 AGTTRDAIDTPFTRDGQEYVIIDTAGMRKRGKVYESTERYSVMRAQKAIERSNVVLVVLD 266 Query: 241 GEEGIIDQDKHIAGYAHDAGKASIFVVNKWDAVEKDDKTMQNFENTIRDHFLFMSYAPVV 300 GEEGII+QDK +AG AH+AGKA + VVNKWDAVEKDDKTM+ E IR FLF+ YAPVV Sbjct: 267 GEEGIIEQDKRVAGLAHEAGKAIVIVVNKWDAVEKDDKTMKKMEEKIRQEFLFLDYAPVV 326 Query: 301 FLSALTKQRLQKLLPVVQHVAQQHALRITTHLVNDVVSDAVAINPPPTDKGRRLR 355 FLSALT +RLQ LLPV++ VA+ H RI+T ++NDV+ DAVA+NP PTDKG+RL+ Sbjct: 327 FLSALTSKRLQTLLPVIKQVAETHRRRISTSVLNDVIVDAVAMNPTPTDKGQRLK 381 Score = 77.0 bits (188), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 48/140 (34%), Positives = 74/140 (52%), Gaps = 3/140 (2%) Query: 152 VALIGRPNVGKSSLVNAILGEERVIVSDVAGTTRDAIDTPFERDGQRYVLIDTAGMRKRG 211 VA++GRPN+GKS++ N I+G+ IV D G TRD I E +++ LIDT G+ Sbjct: 6 VAIVGRPNIGKSTIFNRIIGDRVSIVDDKPGVTRDRIYGTGEWLNRKFHLIDTGGIEVGD 65 Query: 212 KVYETTEKYSVMRAMRAIERADVVLVVINGEEGIIDQDKHIAGYAHDAGKASIFVVNKWD 271 + ++ +A AI+ ADV++ ++NG EGI D+ +A + K + VNK D Sbjct: 66 EPLLVQMRH---QAELAIDEADVIIFMVNGREGITAADEEVANLLFRSNKPIVLAVNKVD 122 Query: 272 AVEKDDKTMQNFENTIRDHF 291 E D + + D F Sbjct: 123 NFEMRDLMYEFYSLGFGDPF 142 >B7GHN9.1 B7GHN9_ANOFW Predicted GTPase Length = 449 Score = 480 bits (1235), Expect = e-133, Method: Compositional matrix adjust. Identities = 229/355 (64%), Positives = 289/355 (81%), Gaps = 1/355 (0%) Query: 1 LAIVEDKPGITRDRIYGVSDWNGKSFSVIDTGGIEIDGEDAILKSIRVQAELAIEEADVI 60 ++IVED PG+TRDRIY ++W +F++IDTGGI+I G++ +L IR QAE+AI+EADVI Sbjct: 41 ISIVEDVPGVTRDRIYSSAEWLNTTFNMIDTGGIDI-GDEPLLTQIRQQAEIAIDEADVI 99 Query: 61 VFMCEAKSGLTNADEEVAQILFRSGKPVVLAINKVDNMKRTEDIYEFYSLGIGDPXXXXX 120 +FM + G+T ADEEVA+IL+RS KPVVLA+NK+DN E+IY+FY+LG G+P Sbjct: 100 IFMTNGRDGVTAADEEVAKILYRSNKPVVLAVNKIDNPDMRENIYDFYTLGFGEPIPISG 159 Query: 121 XXXXXXXDLLDAVTERLPEPVEEEYDDDIIRVALIGRPNVGKSSLVNAILGEERVIVSDV 180 DLLDAV P+ +EYD D+I+ LIGRPNVGKSSLVNAILGEERVIVSD+ Sbjct: 160 THGLGIGDLLDAVVAHFPKRETKEYDADVIKFCLIGRPNVGKSSLVNAILGEERVIVSDI 219 Query: 181 AGTTRDAIDTPFERDGQRYVLIDTAGMRKRGKVYETTEKYSVMRAMRAIERADVVLVVIN 240 AGTTRDAIDT F+RDGQ YV+IDTAGMRKRGKVYE+TEKYSV+RA++AIER+DVVLVVIN Sbjct: 220 AGTTRDAIDTTFKRDGQEYVIIDTAGMRKRGKVYESTEKYSVLRALKAIERSDVVLVVIN 279 Query: 241 GEEGIIDQDKHIAGYAHDAGKASIFVVNKWDAVEKDDKTMQNFENTIRDHFLFMSYAPVV 300 EEGII+QDK IAGYAH+AG+ + VVNKWDA+EKD+KTM FE IRDHF F+ YAP+V Sbjct: 280 AEEGIIEQDKKIAGYAHEAGRGVVIVVNKWDAIEKDEKTMNEFEKNIRDHFQFLDYAPIV 339 Query: 301 FLSALTKQRLQKLLPVVQHVAQQHALRITTHLVNDVVSDAVAINPPPTDKGRRLR 355 F+SA TKQRL KLLPV+Q V++ HA+R+ T+++N+V+ DA+A+NP PT G+RL+ Sbjct: 340 FVSAKTKQRLHKLLPVIQMVSENHAMRVQTNVLNEVLMDAIAMNPTPTHNGQRLK 394 Score = 73.2 bits (178), Expect = 4e-11, Method: Compositional matrix adjust. Identities = 45/120 (37%), Positives = 68/120 (56%), Gaps = 3/120 (2%) Query: 152 VALIGRPNVGKSSLVNAILGEERVIVSDVAGTTRDAIDTPFERDGQRYVLIDTAGMRKRG 211 VA++GRPNVGKS++ N I+GE IV DV G TRD I + E + +IDT G+ Sbjct: 19 VAIVGRPNVGKSTIFNRIVGERISIVEDVPGVTRDRIYSSAEWLNTTFNMIDTGGIDIGD 78 Query: 212 KVYETTEKYSVMRAMRAIERADVVLVVINGEEGIIDQDKHIAGYAHDAGKASIFVVNKWD 271 + T + +A AI+ ADV++ + NG +G+ D+ +A + + K + VNK D Sbjct: 79 EPLLTQIR---QQAEIAIDEADVIIFMTNGRDGVTAADEEVAKILYRSNKPVVLAVNKID 135 >B4BL07.1 B4BL07_9BACI Small GTP-binding protein Length = 436 Score = 471 bits (1211), Expect = e-131, Method: Compositional matrix adjust. Identities = 228/355 (64%), Positives = 287/355 (80%), Gaps = 1/355 (0%) Query: 1 LAIVEDKPGITRDRIYGVSDWNGKSFSVIDTGGIEIDGEDAILKSIRVQAELAIEEADVI 60 ++IVED PG+TRDRIY ++W SF +IDTGGI+I G++ +L IR QAE+AI+EADVI Sbjct: 28 ISIVEDVPGVTRDRIYSSAEWLNHSFYLIDTGGIDI-GDEPLLVQIRQQAEIAIDEADVI 86 Query: 61 VFMCEAKSGLTNADEEVAQILFRSGKPVVLAINKVDNMKRTEDIYEFYSLGIGDPXXXXX 120 +FM + G+T ADEEVA++L RS KPVVLA+NK+DN + + IY+FY+LG G+P Sbjct: 87 IFMTNGRDGVTAADEEVAKLLRRSNKPVVLAVNKIDNPEMRDLIYDFYALGFGEPYPISG 146 Query: 121 XXXXXXXDLLDAVTERLPEPVEEEYDDDIIRVALIGRPNVGKSSLVNAILGEERVIVSDV 180 DLLDAV P+ +EY++D+I+ LIGRPNVGKSSLVNAILGEERVIVSD+ Sbjct: 147 AHGTGLGDLLDAVARHFPKDHGQEYEEDVIKFCLIGRPNVGKSSLVNAILGEERVIVSDI 206 Query: 181 AGTTRDAIDTPFERDGQRYVLIDTAGMRKRGKVYETTEKYSVMRAMRAIERADVVLVVIN 240 AGTTRDA+DT F R+GQ+YV+IDTAGMRKRGK+YE+TEKYSV+RA+RAIER+DVVLVV+N Sbjct: 207 AGTTRDAVDTSFVREGQKYVIIDTAGMRKRGKIYESTEKYSVLRALRAIERSDVVLVVLN 266 Query: 241 GEEGIIDQDKHIAGYAHDAGKASIFVVNKWDAVEKDDKTMQNFENTIRDHFLFMSYAPVV 300 EEGII+QDK IAGYAH+AG+ I VVNKWDAVEKDDKTM FE IRDHF F+ YAP++ Sbjct: 267 AEEGIIEQDKKIAGYAHEAGRGVILVVNKWDAVEKDDKTMVEFERKIRDHFPFLDYAPIL 326 Query: 301 FLSALTKQRLQKLLPVVQHVAQQHALRITTHLVNDVVSDAVAINPPPTDKGRRLR 355 F+SA TKQRL KLLP+VQ V+ HA+R+ T+++N+V+ DAVA+NP PT GRRL+ Sbjct: 327 FVSAKTKQRLHKLLPLVQLVSDNHAMRVQTNVLNEVIMDAVAMNPTPTHNGRRLK 381 Score = 73.9 bits (180), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 47/126 (37%), Positives = 68/126 (53%), Gaps = 3/126 (2%) Query: 152 VALIGRPNVGKSSLVNAILGEERVIVSDVAGTTRDAIDTPFERDGQRYVLIDTAGMRKRG 211 VA++GRPNVGKS++ N I+GE IV DV G TRD I + E + LIDT G+ Sbjct: 6 VAIVGRPNVGKSTIFNRIVGERISIVEDVPGVTRDRIYSSAEWLNHSFYLIDTGGIDIGD 65 Query: 212 KVYETTEKYSVMRAMRAIERADVVLVVINGEEGIIDQDKHIAGYAHDAGKASIFVVNKWD 271 + + +A AI+ ADV++ + NG +G+ D+ +A + K + VNK D Sbjct: 66 EPLLVQIR---QQAEIAIDEADVIIFMTNGRDGVTAADEEVAKLLRRSNKPVVLAVNKID 122 Query: 272 AVEKDD 277 E D Sbjct: 123 NPEMRD 128 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.137 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,110,405,274 Number of extensions: 84844831 Number of successful extensions: 334075 Number of sequences better than 1.0e-05: 4819 Number of HSP's gapped: 326437 Number of HSP's successfully gapped: 7046 Length of query: 375 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 238 Effective length of database: 1,770,017,148 Effective search space: 421264081224 Effective search space used: 421264081224 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)