BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0256.0055.p (340 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KL91.1 B3KL91_9BACI Glycerol-3-phosphate dehydrogenase EC=1.1.... 516 e-144 B1DJ65.1 B1DJ65_9BACL Glycerol-3-phosphate dehydrogenase EC=1.1.... 420 e-116 C0ZCB8.1 C0ZCB8_BREBN Glycerol-3-phosphate dehydrogenase [NAD(P)... 385 e-105 C1PEY7.1 C1PEY7_BACCO Glycerol-3-phosphate dehydrogenase (NAD(P)... 366 2e-99 Q2B621.1 Q2B621_9BACI Glycerol-3-phosphate dehydrogenase EC=1.1.... 364 7e-99 >B3KL91.1 B3KL91_9BACI Glycerol-3-phosphate dehydrogenase EC=1.1.1.94 Length = 346 Score = 516 bits (1328), Expect = e-144, Method: Compositional matrix adjust. Identities = 253/340 (74%), Positives = 280/340 (82%) Query: 1 VLVAGSWGTALASVLAANHPEVYLWTRKPEQAAEINNSHTNQHFLPGITLPPNIIATTDM 60 VLVAGSWGTALASVLA+N +V +WTR EQ EIN HTN +LPG+ L NI ATTDM Sbjct: 7 VLVAGSWGTALASVLASNDLDVVVWTRTEEQTREINELHTNNRYLPGVELSSNIRATTDM 66 Query: 61 ETAVKDSKAVVIVAPSSAMRQVAGALKPFWKEGMLCIHATKGFETETVKRMSTVISEELG 120 E AV + AVVIVAPSSAMR+V+G LK +W E ML IHATKGFE E++KRMSTVISEELG Sbjct: 67 EEAVSSAAAVVIVAPSSAMREVSGQLKQYWSEDMLVIHATKGFEIESLKRMSTVISEELG 126 Query: 121 CSEGEIVVLSGPSHAEEVVRLCPTTVVVXXXXXXXXXXXXXLFINNDFRVYTNRDQVGVE 180 C EG +VVLSGPSHAEEVVR CPTTVVV LF+N FRVYTNRD +GVE Sbjct: 127 CEEGRVVVLSGPSHAEEVVRRCPTTVVVASLDAKAAAAAQDLFMNTYFRVYTNRDMIGVE 186 Query: 181 LAGALKNIIALGAGLSDGLGFGDNAKAALLTRGLAEISRVGVELGANPLTFSGLAGLGDL 240 LAGALKNIIALGAG+SDGLGFGDNAKAAL+TRGLAEI R+G+E+GANPLTF+GLAG+GDL Sbjct: 187 LAGALKNIIALGAGMSDGLGFGDNAKAALITRGLAEILRIGMEMGANPLTFAGLAGIGDL 246 Query: 241 VVTATSKHSRNWRAGSMLGQGKPLAEVLESMGMVVEGIRTTKAAYAISLNLNVQMPITDQ 300 VVTATS+HSRNWRAGSMLGQGK L EVL SMGMVVEGIRTTKAA+AIS VQMPI DQ Sbjct: 247 VVTATSQHSRNWRAGSMLGQGKKLDEVLASMGMVVEGIRTTKAAHAISEKYGVQMPIADQ 306 Query: 301 IYHVLFQGRTPRSAVEALMGRDRKTEMEAISQETWEQWHS 340 +YHVLF+ R PR AVEALMGRD KTEME + ETWEQWH+ Sbjct: 307 LYHVLFKDRNPRVAVEALMGRDPKTEMEMMPLETWEQWHT 346 >B1DJ65.1 B1DJ65_9BACL Glycerol-3-phosphate dehydrogenase EC=1.1.1.94 Length = 348 Score = 420 bits (1080), Expect = e-116, Method: Compositional matrix adjust. Identities = 209/331 (63%), Positives = 253/331 (76%) Query: 1 VLVAGSWGTALASVLAANHPEVYLWTRKPEQAAEINNSHTNQHFLPGITLPPNIIATTDM 60 VLVAGSWGTALA+VLA N +V LWTR E A ++N +H N+ +LPG+ LP ++ ATTDM Sbjct: 18 VLVAGSWGTALAAVLAGNGYDVQLWTRSAEHADQMNANHKNEKYLPGVALPASLHATTDM 77 Query: 61 ETAVKDSKAVVIVAPSSAMRQVAGALKPFWKEGMLCIHATKGFETETVKRMSTVISEELG 120 + A++D + + VAPSSAMR VA P+ E L +HATKGFE +T+KRM+TV+SEEL Sbjct: 78 QEALRDVELALFVAPSSAMRAVAKQAAPYLAEEALVVHATKGFEADTLKRMTTVLSEELN 137 Query: 121 CSEGEIVVLSGPSHAEEVVRLCPTTVVVXXXXXXXXXXXXXLFINNDFRVYTNRDQVGVE 180 +IVVLSGPSHAEEV+R PTTVVV F++ FRVYTN D VGVE Sbjct: 138 RPADKIVVLSGPSHAEEVIRKQPTTVVVASADMDAAEQAQDAFMSTYFRVYTNPDVVGVE 197 Query: 181 LAGALKNIIALGAGLSDGLGFGDNAKAALLTRGLAEISRVGVELGANPLTFSGLAGLGDL 240 +AGA+KNIIALGAGLSDGLGFGDNAKAALLTRGLAEISR+G +GANPLTF+GLAG+GDL Sbjct: 198 VAGAIKNIIALGAGLSDGLGFGDNAKAALLTRGLAEISRLGTAMGANPLTFAGLAGVGDL 257 Query: 241 VVTATSKHSRNWRAGSMLGQGKPLAEVLESMGMVVEGIRTTKAAYAISLNLNVQMPITDQ 300 VVT TS+HSRNWRAGSML G PL EVLE MGMVVEG+RTT+AA ++ +VQMPIT + Sbjct: 258 VVTCTSQHSRNWRAGSMLADGTPLDEVLERMGMVVEGVRTTRAANELAKQYDVQMPITAE 317 Query: 301 IYHVLFQGRTPRSAVEALMGRDRKTEMEAIS 331 +Y VLF +P+SAVE LMGR + E+E +S Sbjct: 318 LYSVLFANNSPKSAVENLMGRLKTHEIEQLS 348 >C0ZCB8.1 C0ZCB8_BREBN Glycerol-3-phosphate dehydrogenase [NAD(P)+] EC=1.1.1.94 Length = 350 Score = 385 bits (988), Expect = e-105, Method: Compositional matrix adjust. Identities = 194/328 (59%), Positives = 240/328 (73%), Gaps = 1/328 (0%) Query: 1 VLVAGSWGTALASVLAANHPEVYLWTRKPEQAAEINNSHTNQHFLPGITLPPNIIATTDM 60 V+ AGSWGTALA+VLA N +V LW R A EIN+ H N+ +LPG L NI+ATT++ Sbjct: 15 VIGAGSWGTALATVLADNGHQVTLWVRDTRLAEEINSKHVNEKYLPGEKLSANIVATTNI 74 Query: 61 ETAVKDSKAVVIVAPSSAMRQVAGALKPFWKEGMLCIHATKGFETETVKRMSTVISEELG 120 + AV + V++V PS AMRQ+ + P + +L IHATKGFE +++KRMS VISEE+ Sbjct: 75 QEAVVGKRIVLLVVPSHAMRQICQQVAPHLEPDVLLIHATKGFELQSLKRMSEVISEEVP 134 Query: 121 CS-EGEIVVLSGPSHAEEVVRLCPTTVVVXXXXXXXXXXXXXLFINNDFRVYTNRDQVGV 179 + VLSGPSHAEEVV+ PTTVV+ L +N +FRVYTN D +G Sbjct: 135 KEIASRLAVLSGPSHAEEVVKRQPTTVVMASESKESMLRSQDLLMNTNFRVYTNPDLIGA 194 Query: 180 ELAGALKNIIALGAGLSDGLGFGDNAKAALLTRGLAEISRVGVELGANPLTFSGLAGLGD 239 E+AGALKNIIALGAG+SDGLG+GDNAKAALLTRGLAEISR+G++LGA PLTFSGLAG+GD Sbjct: 195 EIAGALKNIIALGAGMSDGLGYGDNAKAALLTRGLAEISRLGIKLGAKPLTFSGLAGVGD 254 Query: 240 LVVTATSKHSRNWRAGSMLGQGKPLAEVLESMGMVVEGIRTTKAAYAISLNLNVQMPITD 299 L+ T TSKHSRNWRAG MLGQGK L +VL MGMVVEG+RTT AA+++S V+MPIT Sbjct: 255 LIATCTSKHSRNWRAGYMLGQGKKLDDVLAQMGMVVEGVRTTNAAFSLSQREQVEMPITT 314 Query: 300 QIYHVLFQGRTPRSAVEALMGRDRKTEM 327 +Y +LF+G+ R VE LMGR + EM Sbjct: 315 VLYGMLFEGKEARQGVEELMGRGKTFEM 342 >C1PEY7.1 C1PEY7_BACCO Glycerol-3-phosphate dehydrogenase (NAD(P)(+)) EC=1.1.1.94 Length = 346 Score = 366 bits (940), Expect = 2e-99, Method: Compositional matrix adjust. Identities = 190/339 (56%), Positives = 235/339 (69%), Gaps = 1/339 (0%) Query: 1 VLVAGSWGTALASVLAANHPEVYLWTRKPEQAAEINNSHTNQHFLPGITLPPNIIATTDM 60 VL AGSWGTAL+ VLA N +V LW K Q EIN SHTN +LPGI L I + Sbjct: 8 VLGAGSWGTALSIVLADNGFQVRLWGNKKGQIDEINTSHTNAFYLPGIQLQSAITGIFSL 67 Query: 61 ETAVKDSKAVVIVAPSSAMRQVAGALKPFWKEGMLCIHATKGFETETVKRMSTVISEELG 120 E A+ D V+ P+ A+R V +K K + IH TKG E ++ R+S +I EE+ Sbjct: 68 EEALDDIDTAVLAVPTKAVRDVCRQVKAVRKSPLSIIHVTKGIEPGSLMRISEMIEEEMP 127 Query: 121 CSE-GEIVVLSGPSHAEEVVRLCPTTVVVXXXXXXXXXXXXXLFINNDFRVYTNRDQVGV 179 S ++ VLSGPSHAEEV PTTV V +F+N++FRVYTN D +GV Sbjct: 128 ESLLRDLTVLSGPSHAEEVSLRHPTTVTVSSKKMAAAKKAQDIFMNSNFRVYTNPDIIGV 187 Query: 180 ELAGALKNIIALGAGLSDGLGFGDNAKAALLTRGLAEISRVGVELGANPLTFSGLAGLGD 239 EL G+LKNIIAL AG+SDGLG+GDNAKAAL+TRGLAEI+R+G ++GANPLTF GLAG+GD Sbjct: 188 ELGGSLKNIIALAAGISDGLGYGDNAKAALMTRGLAEIARLGTKMGANPLTFLGLAGIGD 247 Query: 240 LVVTATSKHSRNWRAGSMLGQGKPLAEVLESMGMVVEGIRTTKAAYAISLNLNVQMPITD 299 L+VT TS HSRNWRAG+MLG+GK L +VLE MGMVVEG+RTTKAAY +S V+MPITD Sbjct: 248 LIVTCTSVHSRNWRAGNMLGKGKKLDDVLEQMGMVVEGVRTTKAAYQLSSRYEVKMPITD 307 Query: 300 QIYHVLFQGRTPRSAVEALMGRDRKTEMEAISQETWEQW 338 +YHVLF+G P+ AV+ LMGR +K EME + EQ+ Sbjct: 308 ALYHVLFEGLDPKEAVDNLMGRLKKHEMEGLMDMLGEQY 346 >Q2B621.1 Q2B621_9BACI Glycerol-3-phosphate dehydrogenase EC=1.1.1.94 Length = 350 Score = 364 bits (934), Expect = 7e-99, Method: Compositional matrix adjust. Identities = 181/331 (54%), Positives = 235/331 (70%), Gaps = 3/331 (0%) Query: 4 AGSWGTALASVLAANHPEVYLWTRKPEQAAEINNSHTNQHFLPGITLPPNIIATTDMETA 63 AGSWGTALA VLA N +V LW P Q EIN HTNQ +LP I LP I + +E A Sbjct: 13 AGSWGTALAMVLADNGRQVRLWGHNPVQIKEINEQHTNQKYLPDIKLPSGIQGFSSLEEA 72 Query: 64 VKDSKAVVIVAPSSAMRQVAGALKPFWKEGMLCIHATKGFETETVKRMSTVISEELGCSE 123 + +++ P+ A+R+VAG + F + + +H +KG E +T+ R+S +I EE+ SE Sbjct: 73 LDGVDTLILAVPTKAIREVAGKINEFQRVPLTIVHVSKGIEPDTLLRISEMIEEEI-TSE 131 Query: 124 --GEIVVLSGPSHAEEVVRLCPTTVVVXXXXXXXXXXXXXLFINNDFRVYTNRDQVGVEL 181 G +VVLSGPSHAEEV PTTV V LFIN++FRVYTN D +GVE+ Sbjct: 132 ALGSVVVLSGPSHAEEVSLRHPTTVTVSSKNIEAAEHVQDLFINHNFRVYTNPDIIGVEI 191 Query: 182 AGALKNIIALGAGLSDGLGFGDNAKAALLTRGLAEISRVGVELGANPLTFSGLAGLGDLV 241 GALKNIIAL AG++DGLG+GDNAKAAL+TRGLAEI+R+G ++GA+PLTFSGL G+GDL+ Sbjct: 192 GGALKNIIALAAGITDGLGYGDNAKAALITRGLAEIARLGTKMGASPLTFSGLTGIGDLI 251 Query: 242 VTATSKHSRNWRAGSMLGQGKPLAEVLESMGMVVEGIRTTKAAYAISLNLNVQMPITDQI 301 VT TS HSRNWRAG++LG+G L EVL++MGMVVEG+RTTKAAY ++ NV+MPIT+ + Sbjct: 252 VTCTSVHSRNWRAGNLLGKGHSLQEVLDNMGMVVEGVRTTKAAYQLAEKYNVKMPITEAL 311 Query: 302 YHVLFQGRTPRSAVEALMGRDRKTEMEAISQ 332 Y VLF G P+ +V+ LM R + EME ++ Sbjct: 312 YEVLFNGADPKESVDKLMARGKTHEMEDLAN 342 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.131 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,821,413,517 Number of extensions: 69077576 Number of successful extensions: 206141 Number of sequences better than 1.0e-05: 2040 Number of HSP's gapped: 202601 Number of HSP's successfully gapped: 2082 Length of query: 340 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 204 Effective length of database: 1,779,438,163 Effective search space: 363005385252 Effective search space used: 363005385252 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)