BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0256.0048.p (528 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KL98.1 B3KL98_9BACI Stage IV sporulation protein A 932 0.0 B1DMC6.1 B1DMC6_9BACL Stage IV sporulation protein A 899 0.0 Q5KXT4.1 Q5KXT4_GEOKA Stage IV sporulation protein A (Spore cort... 816 0.0 C3IYS9.1 C3IYS9_9BACI Stage IV sporulation protein A 816 0.0 C0ZCC5.1 C0ZCC5_BREBN Stage IV sporulation protein A 812 0.0 >B3KL98.1 B3KL98_9BACI Stage IV sporulation protein A Length = 492 Score = 932 bits (2408), Expect = 0.0, Method: Compositional matrix adjust. Identities = 452/492 (91%), Positives = 473/492 (96%) Query: 37 LEKVDIFKDIAERTGGDIYLGVVGAVRTGKSTFIKRFMETIVLPNITSEADRVRAIDELP 96 +EKVDIFKDIAERTGGDIYLGVVGAVRTGKSTFIKRFMET+VLPNIT+EADRVRA+DELP Sbjct: 1 MEKVDIFKDIAERTGGDIYLGVVGAVRTGKSTFIKRFMETVVLPNITNEADRVRAVDELP 60 Query: 97 QSAAGKTIMTTEPKFVPNNAVQIKVTEGLEVNVRLVDCVGYAVEGAKGYEDENGPRMIST 156 QSAAGKTIMTTEPKFVPNNAVQIKVT+GLEVNVRLVDCVGYAVEGAKGYEDENGPRMIST Sbjct: 61 QSAAGKTIMTTEPKFVPNNAVQIKVTDGLEVNVRLVDCVGYAVEGAKGYEDENGPRMIST 120 Query: 157 PWFEEPIPFQEAAEIGTRKVIQEHSTLGVVVTTDGTIAEIPRHSYVDSEERVIAELKEVG 216 PWFEEPIPFQEAAEIGTRKVIQEHSTLGVVVTTDG+IAEIPR SYV++EERVI ELKEVG Sbjct: 121 PWFEEPIPFQEAAEIGTRKVIQEHSTLGVVVTTDGSIAEIPRSSYVEAEERVIEELKEVG 180 Query: 217 KPFVLVINSTRPRSEEAQQLRSELAAKYDIPVMTLSAANMSEDDVTGVLREVLYEFPVHE 276 KPFV+VINSTRPRSEEA QLR+ELA KYDIPVMTLSAA M++DDVTGVLREVLYEFPVHE Sbjct: 181 KPFVVVINSTRPRSEEALQLRNELAEKYDIPVMTLSAATMTDDDVTGVLREVLYEFPVHE 240 Query: 277 VNVNLPSWVMVLGENHWLRSSYENSVRDTVKDIXXXXXXXXXXSQFTEYDFIERAGLSGM 336 VNVNLPSWVMVL ENHWLRS+YENSVRDTVKDI SQF EYDFI AGLSGM Sbjct: 241 VNVNLPSWVMVLNENHWLRSNYENSVRDTVKDIRRLRDVDRVVSQFMEYDFINHAGLSGM 300 Query: 337 NMGQGVAEIDLYAPEELYDQVLMEIVGVEIRGKDHLLQLMQDFSHAKREYDRFSEALEMV 396 NMGQGVAEIDL+AP+ELYD++LME+VGVEIRGKDHLLQLMQ+FSHAKREYDRF+EALEMV Sbjct: 301 NMGQGVAEIDLFAPDELYDRILMEVVGVEIRGKDHLLQLMQEFSHAKREYDRFAEALEMV 360 Query: 397 KTTGYGIAAPSLAEMALDEPELIRQGSRFGVRLKATAPSIHMIRVDVESEFSPIIGTEKQ 456 KTTGYGIAAPSLAEMALDEPELIRQG+RFGVRLKATAPSIHMIRVDVESEFSPIIGTEKQ Sbjct: 361 KTTGYGIAAPSLAEMALDEPELIRQGTRFGVRLKATAPSIHMIRVDVESEFSPIIGTEKQ 420 Query: 457 SEELVRYLMQDFENDPIKIWESDIFGRSLHSIVREGIQGKIAMMPDNARYKLQETLGRII 516 SEELVRYLMQDFENDPIKIWESDIFGRSLHSIVREGIQGKIAMMPDNARYKLQETLGRII Sbjct: 421 SEELVRYLMQDFENDPIKIWESDIFGRSLHSIVREGIQGKIAMMPDNARYKLQETLGRII 480 Query: 517 NEGSGGLIAIIL 528 NEGSGGLIAIIL Sbjct: 481 NEGSGGLIAIIL 492 >B1DMC6.1 B1DMC6_9BACL Stage IV sporulation protein A Length = 492 Score = 899 bits (2324), Expect = 0.0, Method: Compositional matrix adjust. Identities = 431/492 (87%), Positives = 466/492 (94%) Query: 37 LEKVDIFKDIAERTGGDIYLGVVGAVRTGKSTFIKRFMETIVLPNITSEADRVRAIDELP 96 +EKVDIFKDIAERTGGDIYLGVVGAVRTGKSTFIKRFMET+VLPNI SEA+RVRA+DELP Sbjct: 1 MEKVDIFKDIAERTGGDIYLGVVGAVRTGKSTFIKRFMETVVLPNIASEAERVRAVDELP 60 Query: 97 QSAAGKTIMTTEPKFVPNNAVQIKVTEGLEVNVRLVDCVGYAVEGAKGYEDENGPRMIST 156 QSAAGKTIMTTEPKFVPN AVQ++V+EGL+VN+RLVDCVGYAVEGAKGYEDENGPRMI+T Sbjct: 61 QSAAGKTIMTTEPKFVPNQAVQLRVSEGLDVNIRLVDCVGYAVEGAKGYEDENGPRMITT 120 Query: 157 PWFEEPIPFQEAAEIGTRKVIQEHSTLGVVVTTDGTIAEIPRHSYVDSEERVIAELKEVG 216 PWF+EPIPFQEAAEIGTRKVIQEHSTLGVVVTTDG+IAEIPR SYV++EERVIAELKEVG Sbjct: 121 PWFDEPIPFQEAAEIGTRKVIQEHSTLGVVVTTDGSIAEIPRASYVEAEERVIAELKEVG 180 Query: 217 KPFVLVINSTRPRSEEAQQLRSELAAKYDIPVMTLSAANMSEDDVTGVLREVLYEFPVHE 276 KPFVL+INSTRPR +EA QLR EL AKYDIPV+T+S A M E++V VLREVLYEFPVHE Sbjct: 181 KPFVLIINSTRPRGDEAIQLRGELQAKYDIPVITMSVATMGEEEVMSVLREVLYEFPVHE 240 Query: 277 VNVNLPSWVMVLGENHWLRSSYENSVRDTVKDIXXXXXXXXXXSQFTEYDFIERAGLSGM 336 VNVNLPSWVMVL ENHWLRS+YENSVRDTVKDI SQF++YDFI RAGLSGM Sbjct: 241 VNVNLPSWVMVLNENHWLRSNYENSVRDTVKDIRRLRDVDRVVSQFSDYDFIARAGLSGM 300 Query: 337 NMGQGVAEIDLYAPEELYDQVLMEIVGVEIRGKDHLLQLMQDFSHAKREYDRFSEALEMV 396 NMGQGVAEIDL+AP+ELYD++LME+VGVEIRGKDHLLQLMQ+FSHAKREYDRF+EALEMV Sbjct: 301 NMGQGVAEIDLFAPDELYDRILMEVVGVEIRGKDHLLQLMQEFSHAKREYDRFAEALEMV 360 Query: 397 KTTGYGIAAPSLAEMALDEPELIRQGSRFGVRLKATAPSIHMIRVDVESEFSPIIGTEKQ 456 K TGYGIAAP+LAEMALDEPELIRQGSRFGVRLKATAPSIHMIRVDVESEF+PIIG+EKQ Sbjct: 361 KATGYGIAAPTLAEMALDEPELIRQGSRFGVRLKATAPSIHMIRVDVESEFAPIIGSEKQ 420 Query: 457 SEELVRYLMQDFENDPIKIWESDIFGRSLHSIVREGIQGKIAMMPDNARYKLQETLGRII 516 +EELVRYLMQDFE DPIKIWESDIFGRSLHS+VREGIQGKIAMMPDNARYKLQETLGRII Sbjct: 421 TEELVRYLMQDFEADPIKIWESDIFGRSLHSLVREGIQGKIAMMPDNARYKLQETLGRII 480 Query: 517 NEGSGGLIAIIL 528 NEGSGGLIAIIL Sbjct: 481 NEGSGGLIAIIL 492 >Q5KXT4.1 Q5KXT4_GEOKA Stage IV sporulation protein A (Spore cortex formation and coat assembly) Length = 492 Score = 816 bits (2107), Expect = 0.0, Method: Compositional matrix adjust. Identities = 387/492 (78%), Positives = 436/492 (88%) Query: 37 LEKVDIFKDIAERTGGDIYLGVVGAVRTGKSTFIKRFMETIVLPNITSEADRVRAIDELP 96 +EKVDIFKDIAERTGGDIYLGVVGAVRTGKSTFIKRFME +V+PNI +EAD+ RA DELP Sbjct: 1 MEKVDIFKDIAERTGGDIYLGVVGAVRTGKSTFIKRFMELVVIPNIKNEADKARAQDELP 60 Query: 97 QSAAGKTIMTTEPKFVPNNAVQIKVTEGLEVNVRLVDCVGYAVEGAKGYEDENGPRMIST 156 QSAAGKTIMTTEPKFVPN AV +KV EGLEVN+RLVDCVGYAV GAKGYEDENGPRMI T Sbjct: 61 QSAAGKTIMTTEPKFVPNQAVTVKVDEGLEVNIRLVDCVGYAVPGAKGYEDENGPRMIHT 120 Query: 157 PWFEEPIPFQEAAEIGTRKVIQEHSTLGVVVTTDGTIAEIPRHSYVDSEERVIAELKEVG 216 PW+EEPIPFQEAAEIGTRKVIQEHST+GVV+TTDGTI EIPR YV++EERVI+ELKEVG Sbjct: 121 PWYEEPIPFQEAAEIGTRKVIQEHSTIGVVITTDGTIGEIPRQDYVEAEERVISELKEVG 180 Query: 217 KPFVLVINSTRPRSEEAQQLRSELAAKYDIPVMTLSAANMSEDDVTGVLREVLYEFPVHE 276 KPF++++N+ RP E + LR ELA KYDIPV+ +S +M E DV VLRE LYEFPV E Sbjct: 181 KPFIMIVNTVRPHHPETEALRRELAEKYDIPVLAMSVESMREADVYNVLREALYEFPVLE 240 Query: 277 VNVNLPSWVMVLGENHWLRSSYENSVRDTVKDIXXXXXXXXXXSQFTEYDFIERAGLSGM 336 VNVNLPSWVMVL E+HWLR SY+ +VRDTVKDI QF EYDFIE+A L+G+ Sbjct: 241 VNVNLPSWVMVLREDHWLRESYQEAVRDTVKDIKRLRDVDRVVQQFAEYDFIEKAALAGI 300 Query: 337 NMGQGVAEIDLYAPEELYDQVLMEIVGVEIRGKDHLLQLMQDFSHAKREYDRFSEALEMV 396 MGQG+AEIDLYAP+ELYDQ+L EIVGVEIRGKDHLLQLMQDF+HAK EYD+ ++AL+MV Sbjct: 301 EMGQGIAEIDLYAPDELYDQILKEIVGVEIRGKDHLLQLMQDFAHAKAEYDQIADALKMV 360 Query: 397 KTTGYGIAAPSLAEMALDEPELIRQGSRFGVRLKATAPSIHMIRVDVESEFSPIIGTEKQ 456 K TGYGIAAP+L++M+LDEPE+IRQGSRFGVRLKA APSIHMI+VDVESEF+PIIGTEKQ Sbjct: 361 KQTGYGIAAPALSDMSLDEPEIIRQGSRFGVRLKAVAPSIHMIKVDVESEFAPIIGTEKQ 420 Query: 457 SEELVRYLMQDFENDPIKIWESDIFGRSLHSIVREGIQGKIAMMPDNARYKLQETLGRII 516 SEELVRYLMQDFE+DP+ IW SDIFGRSL SIVREGIQ K+A+MP+NARYKL+ETL RII Sbjct: 421 SEELVRYLMQDFEDDPLSIWNSDIFGRSLSSIVREGIQAKLALMPENARYKLKETLERII 480 Query: 517 NEGSGGLIAIIL 528 NEGSGGLIAIIL Sbjct: 481 NEGSGGLIAIIL 492 >C3IYS9.1 C3IYS9_9BACI Stage IV sporulation protein A Length = 492 Score = 816 bits (2107), Expect = 0.0, Method: Compositional matrix adjust. Identities = 387/492 (78%), Positives = 436/492 (88%) Query: 37 LEKVDIFKDIAERTGGDIYLGVVGAVRTGKSTFIKRFMETIVLPNITSEADRVRAIDELP 96 +EKVDIFKDIAERTGGDIYLGVVGAVRTGKSTFIKRFME +V+PNI +EAD+ RA DELP Sbjct: 1 MEKVDIFKDIAERTGGDIYLGVVGAVRTGKSTFIKRFMELVVIPNIKNEADKARAQDELP 60 Query: 97 QSAAGKTIMTTEPKFVPNNAVQIKVTEGLEVNVRLVDCVGYAVEGAKGYEDENGPRMIST 156 QSAAGKTIMTTEPKFVPN AV +KV EGLEVN+RLVDCVGYAV GAKGYEDENGPRMI T Sbjct: 61 QSAAGKTIMTTEPKFVPNQAVTVKVDEGLEVNIRLVDCVGYAVPGAKGYEDENGPRMIHT 120 Query: 157 PWFEEPIPFQEAAEIGTRKVIQEHSTLGVVVTTDGTIAEIPRHSYVDSEERVIAELKEVG 216 PW+EEPIPFQEAAEIGTRKVIQEHST+GVV+TTDGTI EIPR YV++EERVI+ELKEVG Sbjct: 121 PWYEEPIPFQEAAEIGTRKVIQEHSTIGVVITTDGTIGEIPRQDYVEAEERVISELKEVG 180 Query: 217 KPFVLVINSTRPRSEEAQQLRSELAAKYDIPVMTLSAANMSEDDVTGVLREVLYEFPVHE 276 KPF++++N+ RP E + LR ELA KYDIPV+ +S +M E DV VLRE LYEFPV E Sbjct: 181 KPFIMIVNTVRPHHPETEALRRELAEKYDIPVLAMSVESMREADVYNVLREALYEFPVLE 240 Query: 277 VNVNLPSWVMVLGENHWLRSSYENSVRDTVKDIXXXXXXXXXXSQFTEYDFIERAGLSGM 336 VNVNLPSWVMVL E+HWLR SY+ +VRDTVKDI QF EYDFIE+A L+G+ Sbjct: 241 VNVNLPSWVMVLREDHWLRESYQEAVRDTVKDIKRLRDVDRVVQQFAEYDFIEKAALAGI 300 Query: 337 NMGQGVAEIDLYAPEELYDQVLMEIVGVEIRGKDHLLQLMQDFSHAKREYDRFSEALEMV 396 MGQG+AEIDLYAP+ELYDQ+L EIVGVEIRGKDHLLQLMQDF+HAK EYD+ ++AL+MV Sbjct: 301 EMGQGIAEIDLYAPDELYDQILKEIVGVEIRGKDHLLQLMQDFAHAKAEYDQIADALKMV 360 Query: 397 KTTGYGIAAPSLAEMALDEPELIRQGSRFGVRLKATAPSIHMIRVDVESEFSPIIGTEKQ 456 K TGYGIAAP+L++M+LDEPE+IRQGSRFGVRLKA APSIHMI+VDVESEF+PIIGTEKQ Sbjct: 361 KQTGYGIAAPALSDMSLDEPEIIRQGSRFGVRLKAVAPSIHMIKVDVESEFAPIIGTEKQ 420 Query: 457 SEELVRYLMQDFENDPIKIWESDIFGRSLHSIVREGIQGKIAMMPDNARYKLQETLGRII 516 SEELVRYLMQDFE+DP+ IW SDIFGRSL SIVREGIQ K+A+MP+NARYKL+ETL RII Sbjct: 421 SEELVRYLMQDFEDDPLSIWNSDIFGRSLSSIVREGIQAKLALMPENARYKLKETLERII 480 Query: 517 NEGSGGLIAIIL 528 NEGSGGLIAIIL Sbjct: 481 NEGSGGLIAIIL 492 >C0ZCC5.1 C0ZCC5_BREBN Stage IV sporulation protein A Length = 493 Score = 812 bits (2098), Expect = 0.0, Method: Compositional matrix adjust. Identities = 379/493 (76%), Positives = 441/493 (89%) Query: 36 LLEKVDIFKDIAERTGGDIYLGVVGAVRTGKSTFIKRFMETIVLPNITSEADRVRAIDEL 95 ++E+VDIFKDIAERTGGDIYLGVVG VRTGKSTFIKRFME +V+PNI SEA+R+RA DEL Sbjct: 1 MVERVDIFKDIAERTGGDIYLGVVGPVRTGKSTFIKRFMEQVVIPNINSEAERIRATDEL 60 Query: 96 PQSAAGKTIMTTEPKFVPNNAVQIKVTEGLEVNVRLVDCVGYAVEGAKGYEDENGPRMIS 155 PQSA+G+TIMTTEPKFVPN AV + VTEGL +NVRLVDCVGY VEGAKG+ED+NGPRM++ Sbjct: 61 PQSASGRTIMTTEPKFVPNQAVNVHVTEGLSINVRLVDCVGYTVEGAKGFEDDNGPRMVN 120 Query: 156 TPWFEEPIPFQEAAEIGTRKVIQEHSTLGVVVTTDGTIAEIPRHSYVDSEERVIAELKEV 215 TPW+EEP+PF+EAAE+GTRKVIQEHST+G+VVTTDG+IAEIPR Y+D+EERV+ ELKEV Sbjct: 121 TPWYEEPVPFEEAAEVGTRKVIQEHSTIGIVVTTDGSIAEIPRVGYIDAEERVVNELKEV 180 Query: 216 GKPFVLVINSTRPRSEEAQQLRSELAAKYDIPVMTLSAANMSEDDVTGVLREVLYEFPVH 275 GKPFV+V+NSTRPRS+EAQ LR +L KYD+PV+ LS +M+E ++ ++REVL+EFPVH Sbjct: 181 GKPFVIVVNSTRPRSDEAQNLRVQLQEKYDVPVVALSVDHMTEQEILLLMREVLFEFPVH 240 Query: 276 EVNVNLPSWVMVLGENHWLRSSYENSVRDTVKDIXXXXXXXXXXSQFTEYDFIERAGLSG 335 EVNVNLPSWVMVL HWLR +YE +VR+TV+DI F +YDF+ERA L+G Sbjct: 241 EVNVNLPSWVMVLENGHWLRQNYEEAVRETVQDIRRLRDVDRVVGFFNDYDFVERASLAG 300 Query: 336 MNMGQGVAEIDLYAPEELYDQVLMEIVGVEIRGKDHLLQLMQDFSHAKREYDRFSEALEM 395 M+MGQG+AEIDLYAP+ELYD++LMEIVGVEI GKDHLL++MQ+F+HAKRE+D+ +EAL M Sbjct: 301 MHMGQGIAEIDLYAPDELYDRILMEIVGVEINGKDHLLKIMQEFAHAKREFDQVAEALHM 360 Query: 396 VKTTGYGIAAPSLAEMALDEPELIRQGSRFGVRLKATAPSIHMIRVDVESEFSPIIGTEK 455 V++TGYGIAAPSL EM LDEPELIRQG RFGVRLKATAPSIHMIRVDVESEF+PIIGTEK Sbjct: 361 VRSTGYGIAAPSLHEMTLDEPELIRQGPRFGVRLKATAPSIHMIRVDVESEFAPIIGTEK 420 Query: 456 QSEELVRYLMQDFENDPIKIWESDIFGRSLHSIVREGIQGKIAMMPDNARYKLQETLGRI 515 QSEELVRYLMQDF+ DP+ IW SDIFGRSLHSIVREGIQ KI MMPDNARYKLQETLGRI Sbjct: 421 QSEELVRYLMQDFDKDPLSIWNSDIFGRSLHSIVREGIQAKITMMPDNARYKLQETLGRI 480 Query: 516 INEGSGGLIAIIL 528 INEGSGGLIAIIL Sbjct: 481 INEGSGGLIAIIL 493 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.135 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,980,019,511 Number of extensions: 122834973 Number of successful extensions: 361797 Number of sequences better than 1.0e-05: 197 Number of HSP's gapped: 361707 Number of HSP's successfully gapped: 197 Length of query: 528 Length of database: 3,060,696,203 Length adjustment: 141 Effective length of query: 387 Effective length of database: 1,732,333,088 Effective search space: 670412905056 Effective search space used: 670412905056 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 134 (56.2 bits)