BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0256.0043.p (250 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KLR2.1 B3KLR2_9BACI Ubiquinone/menaquinone biosynthesis methyl... 394 e-108 B1DMB8.1 B1DMB8_9BACL Ubiquinone/menaquinone biosynthesis methyl... 348 3e-94 Q73AY2.1 UBIE_BACC1 Menaquinone biosynthesis methyltransferase u... 282 2e-74 C2PCN1.1 C2PCN1_BACCE Menaquinone biosynthesis methyltransferase... 280 6e-74 C3C018.1 C3C018_BACTU Menaquinone biosynthesis methyltransferase... 280 7e-74 >B3KLR2.1 B3KLR2_9BACI Ubiquinone/menaquinone biosynthesis methyltransferase Length = 252 Score = 394 bits (1011), Expect = e-108, Method: Compositional matrix adjust. Identities = 181/246 (73%), Positives = 211/246 (85%) Query: 5 KTTTIGDQGTKPKEQFVHSVFESIAGKYDLMNDILSFRRHKAWRKFTMRKMGMKRGDSAV 64 K I G PKEQ+VHSVFESI+GKYDLMNDILSFRRHKAWR++TM+KM M+ GD+A+ Sbjct: 7 KLDAIKHDGKNPKEQYVHSVFESISGKYDLMNDILSFRRHKAWRRYTMKKMNMRHGDTAI 66 Query: 65 DLCCGTCDWSIALAEASKTGCVMGLDFSAGMLEVGRRKVEARNLQDRISLVQGNAMELPF 124 DLCCGTCDW+I++A+AS++G ++GLDFS GML VGR KV L+ +I LVQGNAM LPF Sbjct: 67 DLCCGTCDWTISMAQASESGHIVGLDFSEGMLNVGREKVAKHGLEPQIELVQGNAMSLPF 126 Query: 125 GDNSFDYATIGFGLRNVPDPVRVLNEMKRVVKPGGMVVCLELSKPMKQPFKGIYYFYFQR 184 DN FDYATIGFGLRNVPD ++VL EMKRVVKPGGMVVCLELSKP QPFKGIYYFYFQ+ Sbjct: 127 EDNQFDYATIGFGLRNVPDYMQVLREMKRVVKPGGMVVCLELSKPTWQPFKGIYYFYFQQ 186 Query: 185 VLPLLGKLFAKRYEQYKWLPESLALFPDRKQLEVIFRETGLQKVESFPLTGGIAALHIGL 244 VLP +GKL AKRYEQYKWLPESLALFP R++L FR+TGLQ+V++FPLTGGIAALHIG+ Sbjct: 187 VLPRMGKLVAKRYEQYKWLPESLALFPGREELAEAFRQTGLQQVQAFPLTGGIAALHIGI 246 Query: 245 KENCNA 250 KEN + Sbjct: 247 KENLDV 252 >B1DMB8.1 B1DMB8_9BACL Ubiquinone/menaquinone biosynthesis methyltransferase Length = 248 Score = 348 bits (892), Expect = 3e-94, Method: Compositional matrix adjust. Identities = 157/229 (68%), Positives = 195/229 (85%) Query: 18 EQFVHSVFESIAGKYDLMNDILSFRRHKAWRKFTMRKMGMKRGDSAVDLCCGTCDWSIAL 77 ++ VH++FE+IA KYD+MND+LSFRRHKAWRKFTMRKM +++G +A+DLCCGTCDW+IAL Sbjct: 16 KEHVHALFENIAPKYDMMNDLLSFRRHKAWRKFTMRKMSVQQGQTALDLCCGTCDWTIAL 75 Query: 78 AEASKTGCVMGLDFSAGMLEVGRRKVEARNLQDRISLVQGNAMELPFGDNSFDYATIGFG 137 A+AS TG V GLDFS ML+VG KV L +I+L+QGNAMELPF DNSFD+ TIGFG Sbjct: 76 AKASGTGQVTGLDFSQNMLDVGSVKVNGEGLNKQITLIQGNAMELPFADNSFDFVTIGFG 135 Query: 138 LRNVPDPVRVLNEMKRVVKPGGMVVCLELSKPMKQPFKGIYYFYFQRVLPLLGKLFAKRY 197 LRNVPD ++VL EM+RVVKPGG VVCLELSKP+ QPFKG+YY YF+R+LP +GK AKR+ Sbjct: 136 LRNVPDYLQVLKEMERVVKPGGKVVCLELSKPLWQPFKGMYYLYFERMLPTIGKWVAKRF 195 Query: 198 EQYKWLPESLALFPDRKQLEVIFRETGLQKVESFPLTGGIAALHIGLKE 246 ++YKWLP+SL LFPD QL +FR+ GL++V+++PLTGGIAALHIG KE Sbjct: 196 DEYKWLPDSLKLFPDANQLSELFRQAGLREVQAYPLTGGIAALHIGTKE 244 >Q73AY2.1 UBIE_BACC1 Menaquinone biosynthesis methyltransferase ubiE EC=2.1.1.- Length = 237 Score = 282 bits (722), Expect = 2e-74, Method: Compositional matrix adjust. Identities = 134/232 (57%), Positives = 174/232 (75%), Gaps = 2/232 (0%) Query: 15 KPKEQFVHSVFESIAGKYDLMNDILSFRRHKAWRKFTMRKMGMKRGDSAVDLCCGTCDWS 74 + KE+ VH VFE I+ KYD+MN ++SF+RHKAWRK TMR M +K G A+D+CCGT DW+ Sbjct: 3 QSKEERVHDVFEKISDKYDVMNSVISFQRHKAWRKETMRIMDVKPGSKALDVCCGTADWT 62 Query: 75 IALAEA-SKTGCVMGLDFSAGMLEVGRRKVEARNLQDRISLVQGNAMELPFGDNSFDYAT 133 IALAEA + G V+GLDFS ML VG++KVEA L+ ++ L+ GNAMELPF DN+FDY T Sbjct: 63 IALAEAVGEQGKVVGLDFSENMLSVGKQKVEALQLK-QVELLHGNAMELPFEDNTFDYVT 121 Query: 134 IGFGLRNVPDPVRVLNEMKRVVKPGGMVVCLELSKPMKQPFKGIYYFYFQRVLPLLGKLF 193 IGFGLRNVPD + VL EM RVVKPGG V+CLE S+P F+ Y YF+ ++PL GKLF Sbjct: 122 IGFGLRNVPDYMHVLKEMTRVVKPGGKVICLETSQPTMIGFRQGYILYFKYIMPLFGKLF 181 Query: 194 AKRYEQYKWLPESLALFPDRKQLEVIFRETGLQKVESFPLTGGIAALHIGLK 245 AK Y++Y WL ES + FP K+L +F + GL++V+ P T G+AA+H+G+K Sbjct: 182 AKSYKEYSWLQESASTFPGMKELANMFEKAGLERVQVKPFTFGVAAMHLGMK 233 >C2PCN1.1 C2PCN1_BACCE Menaquinone biosynthesis methyltransferase ubiE Length = 243 Score = 280 bits (717), Expect = 6e-74, Method: Compositional matrix adjust. Identities = 134/237 (56%), Positives = 175/237 (73%), Gaps = 2/237 (0%) Query: 10 GDQGTKPKEQFVHSVFESIAGKYDLMNDILSFRRHKAWRKFTMRKMGMKRGDSAVDLCCG 69 G + KE+ VH VFE I+ KYD+MN ++SF+RHKAWRK TMR M +K G+ A+D+CCG Sbjct: 4 GTSMQQSKEERVHDVFEKISDKYDVMNSVISFQRHKAWRKETMRIMDVKPGNKALDVCCG 63 Query: 70 TCDWSIALAEA-SKTGCVMGLDFSAGMLEVGRRKVEARNLQDRISLVQGNAMELPFGDNS 128 T DW+IALA A + G V+GLDFS ML VG++KVEA L+ ++ L+ GNAMELPF DN+ Sbjct: 64 TADWTIALAGAVGEQGKVVGLDFSENMLSVGKQKVEALQLK-QVELLHGNAMELPFEDNT 122 Query: 129 FDYATIGFGLRNVPDPVRVLNEMKRVVKPGGMVVCLELSKPMKQPFKGIYYFYFQRVLPL 188 FDY TIGFGLRNVPD + VL EM RVVKPGG V+CLE S+P F+ Y YF+ ++PL Sbjct: 123 FDYVTIGFGLRNVPDYMHVLKEMTRVVKPGGKVICLETSQPTMIGFRQGYILYFKYIMPL 182 Query: 189 LGKLFAKRYEQYKWLPESLALFPDRKQLEVIFRETGLQKVESFPLTGGIAALHIGLK 245 GKLFAK +++Y WL ES + FP K+L +F E GL++V+ P T G+AA+H+G+K Sbjct: 183 FGKLFAKSFKEYSWLQESASTFPGMKELAHMFEEAGLERVQVKPFTFGVAAMHLGMK 239 >C3C018.1 C3C018_BACTU Menaquinone biosynthesis methyltransferase ubiE Length = 243 Score = 280 bits (716), Expect = 7e-74, Method: Compositional matrix adjust. Identities = 134/237 (56%), Positives = 175/237 (73%), Gaps = 2/237 (0%) Query: 10 GDQGTKPKEQFVHSVFESIAGKYDLMNDILSFRRHKAWRKFTMRKMGMKRGDSAVDLCCG 69 G + KE+ VH VFE I+ KYD+MN ++SF+RHKAWRK TMR M +K G+ A+D+CCG Sbjct: 4 GTSMQQSKEERVHDVFEKISDKYDVMNSVISFQRHKAWRKETMRIMDVKPGNKALDVCCG 63 Query: 70 TCDWSIALAEA-SKTGCVMGLDFSAGMLEVGRRKVEARNLQDRISLVQGNAMELPFGDNS 128 T DW+IALA A + G V+GLDFS ML VG++KVEA L+ ++ L+ GNAMELPF DN+ Sbjct: 64 TADWTIALAGAVGEQGKVVGLDFSENMLSVGKQKVEALQLK-QVELLHGNAMELPFEDNT 122 Query: 129 FDYATIGFGLRNVPDPVRVLNEMKRVVKPGGMVVCLELSKPMKQPFKGIYYFYFQRVLPL 188 FDY TIGFGLRNVPD + VL EM RVVKPGG V+CLE S+P F+ Y YF+ ++PL Sbjct: 123 FDYVTIGFGLRNVPDYMHVLKEMTRVVKPGGKVICLETSQPTMIGFRQGYILYFKYIMPL 182 Query: 189 LGKLFAKRYEQYKWLPESLALFPDRKQLEVIFRETGLQKVESFPLTGGIAALHIGLK 245 GKLFAK Y++Y WL ES + FP K+L +F + GL++V+ P T G+AA+H+G+K Sbjct: 183 FGKLFAKSYKEYSWLQESASTFPGMKELANMFEKAGLERVQVKPFTFGVAAMHLGMK 239 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.323 0.139 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,479,350,716 Number of extensions: 58134114 Number of successful extensions: 134063 Number of sequences better than 1.0e-05: 2152 Number of HSP's gapped: 133660 Number of HSP's successfully gapped: 2154 Length of query: 250 Length of database: 3,060,696,203 Length adjustment: 132 Effective length of query: 118 Effective length of database: 1,817,122,223 Effective search space: 214420422314 Effective search space used: 214420422314 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 129 (54.3 bits)