BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0256.0041.p (200 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KLR4.1 B3KLR4_9BACI 3-octaprenyl-4-hydroxybenzoate carboxy-lyase 310 4e-83 B1DMB6.1 B1DMB6_9BACL 3-octaprenyl-4-hydroxybenzoate carboxy-lyase 278 3e-73 P94300.1 PAAD_BACPF Probable aromatic acid decarboxylase EC=4.1.1.- 259 8e-68 C0ZCD3.1 C0ZCD3_BREBN Probable aromatic acid decarboxylase EC=4.... 253 6e-66 Q9KCC2.1 PAAD_BACHD Probable aromatic acid decarboxylase EC=4.1.1.- 245 2e-63 >B3KLR4.1 B3KLR4_9BACI 3-octaprenyl-4-hydroxybenzoate carboxy-lyase Length = 200 Score = 310 bits (795), Expect = 4e-83, Method: Compositional matrix adjust. Identities = 144/200 (72%), Positives = 173/200 (86%) Query: 1 MNELKPKNYVVGITGASGGIYGVRLARTLLALGHSVHLVVSNAGWRVFKEELGFAASDRE 60 M ++ K++VVGITGASG IYGVRL TLL+LG+ V L++SNAGWRV KEE+GF A +RE Sbjct: 1 MKSMRRKSWVVGITGASGSIYGVRLTETLLSLGYDVQLIISNAGWRVLKEEMGFQAGNRE 60 Query: 61 GFLNEQFGSYPGSLHYHPVADIGASIASGSFRTEAMIIMPCSMGTLSAVAHGSSDNLMTR 120 L E+FG + G++ YHP++DIGA+IASGS+ E MIIMPCSMGTLS+VAHGSSDNLM R Sbjct: 61 EVLAEKFGGFEGNVIYHPISDIGATIASGSYLVEGMIIMPCSMGTLSSVAHGSSDNLMGR 120 Query: 121 AADVMLKEGRPLVLVPRETPLHAIHLENMLKLSRLGVKMIPAMPAFYYGPTTVDDLVDFM 180 AADVM+KEGRPLVLVPRETPLHAIHLENML L++LGVK+IPAMPAFY+GP T+DD+V F+ Sbjct: 121 AADVMMKEGRPLVLVPRETPLHAIHLENMLTLAKLGVKIIPAMPAFYHGPKTIDDIVHFL 180 Query: 181 VGKVLDSLNIEHSLFRRWGE 200 VGKVLDSL IEH L+ RWG+ Sbjct: 181 VGKVLDSLRIEHELYTRWGD 200 >B1DMB6.1 B1DMB6_9BACL 3-octaprenyl-4-hydroxybenzoate carboxy-lyase Length = 209 Score = 278 bits (710), Expect = 3e-73, Method: Compositional matrix adjust. Identities = 129/198 (65%), Positives = 163/198 (82%), Gaps = 2/198 (1%) Query: 5 KPKNYVVGITGASGGIYGVRLARTLLALGHSVHLVVSNAGWRVFKEELGFAASDREGFLN 64 +PK +VVGITGASG IYG+RL LL + VH+VV+ AGWRV KEELG+ S R+ L Sbjct: 9 RPKRWVVGITGASGAIYGIRLIEELLQANYVVHVVVTEAGWRVLKEELGWDTSRRQAALE 68 Query: 65 EQFGS--YPGSLHYHPVADIGASIASGSFRTEAMIIMPCSMGTLSAVAHGSSDNLMTRAA 122 ++FG G L +HP ADIGASIASGSF+ E M I+PCSMG+L+++A G SD+L+TRAA Sbjct: 69 QRFGEAVTAGRLVFHPNADIGASIASGSFQVEGMAIVPCSMGSLASMARGISDDLLTRAA 128 Query: 123 DVMLKEGRPLVLVPRETPLHAIHLENMLKLSRLGVKMIPAMPAFYYGPTTVDDLVDFMVG 182 DVMLKEGR L++VPRETPLHAIHLENML L+RLGV++IPAMPAFYY P ++D++++F+VG Sbjct: 129 DVMLKEGRKLIIVPRETPLHAIHLENMLTLARLGVRIIPAMPAFYYKPQSMDEMINFLVG 188 Query: 183 KVLDSLNIEHSLFRRWGE 200 KVLDS++IEH LFRRWG+ Sbjct: 189 KVLDSMSIEHQLFRRWGD 206 >P94300.1 PAAD_BACPF Probable aromatic acid decarboxylase EC=4.1.1.- Length = 200 Score = 259 bits (663), Expect = 8e-68, Method: Compositional matrix adjust. Identities = 121/200 (60%), Positives = 153/200 (76%) Query: 1 MNELKPKNYVVGITGASGGIYGVRLARTLLALGHSVHLVVSNAGWRVFKEELGFAASDRE 60 M+E K VGITGASGG+YGVRL + LL + VHLV++ A W+VFKEEL +DR+ Sbjct: 1 MSERIEKIMTVGITGASGGMYGVRLTQELLRQEYKVHLVLTEAAWQVFKEELLLDTTDRQ 60 Query: 61 GFLNEQFGSYPGSLHYHPVADIGASIASGSFRTEAMIIMPCSMGTLSAVAHGSSDNLMTR 120 ++E FG PG LH H + D A IASGS+R+ M+I+PCSMGTLS +AHG+S NL+ R Sbjct: 61 KVIHELFGDLPGELHTHDLHDYAAPIASGSYRSAGMVIIPCSMGTLSGMAHGASGNLLER 120 Query: 121 AADVMLKEGRPLVLVPRETPLHAIHLENMLKLSRLGVKMIPAMPAFYYGPTTVDDLVDFM 180 ADVMLKE R LV+VPRETPLH IHLENMLKLS++G ++PAMP +Y+ P T+DDL++F+ Sbjct: 121 TADVMLKEKRKLVIVPRETPLHDIHLENMLKLSKMGATILPAMPGYYHLPKTIDDLINFL 180 Query: 181 VGKVLDSLNIEHSLFRRWGE 200 VGK LDSL +EH+LF RWGE Sbjct: 181 VGKALDSLGVEHTLFTRWGE 200 >C0ZCD3.1 C0ZCD3_BREBN Probable aromatic acid decarboxylase EC=4.1.1.- Length = 198 Score = 253 bits (646), Expect = 6e-66, Method: Compositional matrix adjust. Identities = 115/198 (58%), Positives = 152/198 (76%), Gaps = 1/198 (0%) Query: 4 LKPKNYVVGITGASGGIYGVRLARTLLALGHSVHLVVSNAGWRVFKEELGFAASDREGFL 63 + + + VGITGASG IYGVR+ + LL GH VHL+++ AGW+VF++EL + DRE L Sbjct: 1 MNNQKWAVGITGASGAIYGVRVVQELLRAGHIVHLMITEAGWQVFRDELDWHTDDREALL 60 Query: 64 NEQFGS-YPGSLHYHPVADIGASIASGSFRTEAMIIMPCSMGTLSAVAHGSSDNLMTRAA 122 E+ + G LHY + D ASGS+R + MI++PCSMGT+S +AHG+S NL+ R A Sbjct: 61 QEKLQQDFSGELHYWGLRDFNCPAASGSYRCDGMIVVPCSMGTVSGIAHGASGNLLERVA 120 Query: 123 DVMLKEGRPLVLVPRETPLHAIHLENMLKLSRLGVKMIPAMPAFYYGPTTVDDLVDFMVG 182 DVM+KEGR LV+VPRETPL+AI LENMLKLSRLGVK++PAMP +Y P T+DDL++F+VG Sbjct: 121 DVMIKEGRRLVIVPRETPLNAIQLENMLKLSRLGVKILPAMPGYYQKPQTMDDLINFVVG 180 Query: 183 KVLDSLNIEHSLFRRWGE 200 K LD++++ HSLFRRWGE Sbjct: 181 KTLDAIDVPHSLFRRWGE 198 >Q9KCC2.1 PAAD_BACHD Probable aromatic acid decarboxylase EC=4.1.1.- Length = 206 Score = 245 bits (625), Expect = 2e-63, Method: Compositional matrix adjust. Identities = 113/192 (58%), Positives = 143/192 (74%) Query: 9 YVVGITGASGGIYGVRLARTLLALGHSVHLVVSNAGWRVFKEELGFAASDREGFLNEQFG 68 Y VGITGASG YG+RL + + G+ VHLV++ AGW+VF+EEL DR+ ++E F Sbjct: 8 YTVGITGASGAQYGIRLVQEMKKKGYKVHLVITEAGWQVFREELLIQTDDRKMVIDELFA 67 Query: 69 SYPGSLHYHPVADIGASIASGSFRTEAMIIMPCSMGTLSAVAHGSSDNLMTRAADVMLKE 128 + G + +H + D A IASGS++ MII+PCSMGTLS +A G+S NL+ R ADVMLKE Sbjct: 68 AGDGEVSFHQLDDYTAPIASGSYQNRGMIIIPCSMGTLSGIAQGASGNLLERTADVMLKE 127 Query: 129 GRPLVLVPRETPLHAIHLENMLKLSRLGVKMIPAMPAFYYGPTTVDDLVDFMVGKVLDSL 188 R LV+VPRETPL+ IHLENMLKLS++GV ++PAMP FY P T+DDL+DF+VGK LD L Sbjct: 128 KRRLVIVPRETPLNQIHLENMLKLSKMGVTILPAMPGFYQLPKTMDDLIDFVVGKALDQL 187 Query: 189 NIEHSLFRRWGE 200 IEH LF RWGE Sbjct: 188 GIEHELFTRWGE 199 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.137 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,191,089,245 Number of extensions: 46518505 Number of successful extensions: 121622 Number of sequences better than 1.0e-05: 924 Number of HSP's gapped: 120743 Number of HSP's successfully gapped: 924 Length of query: 200 Length of database: 3,060,696,203 Length adjustment: 129 Effective length of query: 71 Effective length of database: 1,845,385,268 Effective search space: 131022354028 Effective search space used: 131022354028 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 128 (53.9 bits)