BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0256.0039.p (317 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KLR5.1 B3KLR5_9BACI Polyprenyl synthetase 468 e-130 A4IQ92.1 A4IQ92_GEOTN Component II of heptaprenyl diphosphate sy... 350 1e-94 B4BKZ7.1 B4BKZ7_9BACI Heptaprenyl diphosphate synthase component II 350 1e-94 P55785.1 HEPS2_BACST Heptaprenyl diphosphate synthase component ... 349 2e-94 Q2B611.1 Q2B611_9BACI HepT 348 3e-94 >B3KLR5.1 B3KLR5_9BACI Polyprenyl synthetase Length = 324 Score = 468 bits (1205), Expect = e-130, Method: Compositional matrix adjust. Identities = 232/319 (72%), Positives = 263/319 (82%), Gaps = 2/319 (0%) Query: 1 MFGLLNKDMDQIEKELYRSVQGDDDLLTETSLHLLKAGGKRLRPVFVLMGGKFGQYDLEK 60 +FG L KDMD IE+ELY SV+GD LTETSLHLL+AGGKRLRPVFVLMGGKFG YD++K Sbjct: 6 IFGTLKKDMDVIERELYLSVEGDSPELTETSLHLLRAGGKRLRPVFVLMGGKFGDYDIDK 65 Query: 61 LKRVAIPLELIHSASLVHDDVIDDAELRRGELTVKAKWGDKIAMYTGDYIYAKALVMTSE 120 LK VA+PLELIHSASLVHDDVIDDA+ RRG+ TVKAKW ++IAMYTGDYIY KAL +T+ Sbjct: 66 LKHVAVPLELIHSASLVHDDVIDDADTRRGKPTVKAKWDNRIAMYTGDYIYGKALQLTTH 125 Query: 121 LGNPRIHQILSKAMVEMSIGEMEQIRDFFNSEQSVRHYLRRIRRKTALLIAISCQLGALA 180 L P IHQIL+KAMVEMSIGEMEQIRDFFN+EQ VR YL RIRRKT+LLIA+SCQLGALA Sbjct: 126 LKQPEIHQILAKAMVEMSIGEMEQIRDFFNTEQDVRRYLLRIRRKTSLLIAVSCQLGALA 185 Query: 181 ADAEPNTARLLYDYGYNVGMAFQIRDDLLDLSGTEKQIGKPPGSDMRQGNITLPVIYSLE 240 ADA+ A LY +GYNVGMAFQI+DDLLDL GTEKQIGKPPGSDMRQGNITLPVI++LE Sbjct: 186 ADADRKVANSLYRFGYNVGMAFQIQDDLLDLCGTEKQIGKPPGSDMRQGNITLPVIFALE 245 Query: 241 DQRLRSALLTELASIR--EGNSGVGRAIDLILSGEGIARAEELASRYIAKALEALDQLPN 298 D + R LL EL I G V A+ L+ S EGI+R+E LA RYI KAL ALD LP+ Sbjct: 246 DSQRREPLLQELERIHALNGQCDVSGAVKLVTSSEGISRSEALADRYIGKALAALDTLPD 305 Query: 299 NRTKRNLKDIAFFVTGRSY 317 +TK+ LKDIA F+T RSY Sbjct: 306 IKTKKQLKDIANFITKRSY 324 >A4IQ92.1 A4IQ92_GEOTN Component II of heptaprenyl diphosphate synthase Length = 320 Score = 350 bits (897), Expect = 1e-94, Method: Compositional matrix adjust. Identities = 171/317 (53%), Positives = 232/317 (73%), Gaps = 2/317 (0%) Query: 1 MFGLLNKDMDQIEKELYRSVQGDDDLLTETSLHLLKAGGKRLRPVFVLMGGKFGQYDLEK 60 M+ L+KD+ +E+EL R+VQ + L E +LHLL+AGGKR+RPVFVL+ +FG YDLE+ Sbjct: 6 MYSFLSKDLAAVEQELERTVQSEYGPLGEAALHLLQAGGKRIRPVFVLLAARFGHYDLER 65 Query: 61 LKRVAIPLELIHSASLVHDDVIDDAELRRGELTVKAKWGDKIAMYTGDYIYAKALVMTSE 120 +K VA+ LELIH ASLVHDDVIDDA+LRRG T+KAKW ++ AMYTGDY++A++L +E Sbjct: 66 IKHVAVALELIHMASLVHDDVIDDADLRRGRPTIKAKWSNRFAMYTGDYLFARSLERMAE 125 Query: 121 LGNPRIHQILSKAMVEMSIGEMEQIRDFFNSEQSVRHYLRRIRRKTALLIAISCQLGALA 180 L +PR HQ+L+K +VE+ GE+EQI+D + EQ +R YLRRIRRKTALLIA SCQLGALA Sbjct: 126 LDDPRAHQVLAKTIVEVCRGEIEQIKDKYWFEQPLRTYLRRIRRKTALLIAASCQLGALA 185 Query: 181 ADAEPNTARLLYDYGYNVGMAFQIRDDLLDLSGTEKQIGKPPGSDMRQGNITLPVIYSLE 240 A A + A+ LY +G+ VGM+FQI DD+LD +GTE+Q+GKP GSD+ QGNITLPV+Y+L Sbjct: 186 AGAPESVAKRLYWFGHYVGMSFQITDDILDFTGTEEQLGKPAGSDLLQGNITLPVLYALR 245 Query: 241 DQRLRSALLTELASIREGNSGVGRAIDLILSGEGIARAEELASRYIAKALEALDQLPNNR 300 D+RL++A+ S + I I + I ++ L+ RY+ KAL L +LP N+ Sbjct: 246 DERLKAAITA--VSPETNADEMAAVISAIKRTDAIDQSYALSDRYLEKALRLLGELPANK 303 Query: 301 TKRNLKDIAFFVTGRSY 317 + L D+A ++ R Y Sbjct: 304 ARTLLHDLALYIGKRDY 320 >B4BKZ7.1 B4BKZ7_9BACI Heptaprenyl diphosphate synthase component II Length = 320 Score = 350 bits (897), Expect = 1e-94, Method: Compositional matrix adjust. Identities = 171/317 (53%), Positives = 232/317 (73%), Gaps = 2/317 (0%) Query: 1 MFGLLNKDMDQIEKELYRSVQGDDDLLTETSLHLLKAGGKRLRPVFVLMGGKFGQYDLEK 60 M+ L+KD+ +E+EL R+VQ + L E +LHLL+AGGKR+RPVFVL+ +FG YDLE+ Sbjct: 6 MYSFLSKDLAAVEQELERTVQSEYGPLGEAALHLLQAGGKRIRPVFVLLAARFGHYDLER 65 Query: 61 LKRVAIPLELIHSASLVHDDVIDDAELRRGELTVKAKWGDKIAMYTGDYIYAKALVMTSE 120 +K VA+ LELIH ASLVHDDVIDDA+LRRG T+KAKW ++ AMYTGDY++A++L +E Sbjct: 66 IKHVAVALELIHMASLVHDDVIDDADLRRGRPTIKAKWSNRFAMYTGDYLFARSLERMAE 125 Query: 121 LGNPRIHQILSKAMVEMSIGEMEQIRDFFNSEQSVRHYLRRIRRKTALLIAISCQLGALA 180 L +PR HQ+L+K +VE+ GE+EQI+D + EQ +R YLRRIRRKTALLIA SCQLGALA Sbjct: 126 LDDPRAHQVLAKTIVEVCRGEIEQIKDKYWFEQPLRTYLRRIRRKTALLIAASCQLGALA 185 Query: 181 ADAEPNTARLLYDYGYNVGMAFQIRDDLLDLSGTEKQIGKPPGSDMRQGNITLPVIYSLE 240 A A + A+ LY +G+ VGM+FQI DD+LD +GTE+Q+GKP GSD+ QGNITLPV+Y+L Sbjct: 186 AGAPESVAKRLYWFGHYVGMSFQITDDILDFTGTEEQLGKPAGSDLLQGNITLPVLYALR 245 Query: 241 DQRLRSALLTELASIREGNSGVGRAIDLILSGEGIARAEELASRYIAKALEALDQLPNNR 300 D+RL++A+ S + I I + I ++ L+ RY+ KAL L +LP N+ Sbjct: 246 DERLKAAITA--VSPETNADEMAAVISAIKRTDAIDQSYALSDRYLEKALRLLGELPANK 303 Query: 301 TKRNLKDIAFFVTGRSY 317 + L D+A ++ R Y Sbjct: 304 ARTLLHDLALYIGKRDY 320 >P55785.1 HEPS2_BACST Heptaprenyl diphosphate synthase component 2 EC=2.5.1.30 Length = 320 Score = 349 bits (895), Expect = 2e-94, Method: Compositional matrix adjust. Identities = 172/322 (53%), Positives = 235/322 (72%), Gaps = 12/322 (3%) Query: 1 MFGLLNKDMDQIEKELYRSVQGDDDLLTETSLHLLKAGGKRLRPVFVLMGGKFGQYDLEK 60 M+ L+ D+ +E+EL R+VQ + L E +LHLL+AGGKR+RPVFVL+ +FGQYDLE+ Sbjct: 6 MYSFLSDDLAAVEEELERAVQSEYGPLGEAALHLLQAGGKRIRPVFVLLAARFGQYDLER 65 Query: 61 LKRVAIPLELIHSASLVHDDVIDDAELRRGELTVKAKWGDKIAMYTGDYIYAKALVMTSE 120 +K VA+ LELIH ASLVHDDVIDDA+LRRG T+KAKW ++ AMYTGDY++A++L +E Sbjct: 66 MKHVAVALELIHMASLVHDDVIDDADLRRGRPTIKAKWSNRFAMYTGDYLFARSLERMAE 125 Query: 121 LGNPRIHQILSKAMVEMSIGEMEQIRDFFNSEQSVRHYLRRIRRKTALLIAISCQLGALA 180 LGNPR HQ+L+K +VE+ GE+EQI+D + +Q +R YLRRIRRKTALLIA SCQLGALA Sbjct: 126 LGNPRAHQVLAKTIVEVCRGEIEQIKDKYRFDQPLRTYLRRIRRKTALLIAASCQLGALA 185 Query: 181 ADAEPNTARLLYDYGYNVGMAFQIRDDLLDLSGTEKQIGKPPGSDMRQGNITLPVIYSLE 240 A A + LY +G+ VGM+FQI DD+LD +GTE+Q+GKP GSD+ QGN+TLPV+Y+L Sbjct: 186 AGAPEPIVKRLYWFGHYVGMSFQITDDILDFTGTEEQLGKPAGSDLLQGNVTLPVLYALS 245 Query: 241 DQRLRSALL-----TELASIREGNSGVGRAIDLILSGEGIARAEELASRYIAKALEALDQ 295 D+R+++A+ T++A + S + R + I R+ L+ RY+ KAL LD Sbjct: 246 DERVKAAIAAVGPETDVAEMAAVISAIKRT-------DAIERSYALSDRYLDKALHLLDG 298 Query: 296 LPNNRTKRNLKDIAFFVTGRSY 317 LP N + L+D+A ++ R Y Sbjct: 299 LPMNEARGLLRDLALYIGKRDY 320 >Q2B611.1 Q2B611_9BACI HepT Length = 320 Score = 348 bits (894), Expect = 3e-94, Method: Compositional matrix adjust. Identities = 165/319 (51%), Positives = 236/319 (73%), Gaps = 6/319 (1%) Query: 1 MFGLLNKDMDQIEKELYRSVQGDDDLLTETSLHLLKAGGKRLRPVFVLMGGKFGQYDLEK 60 M+ L+ D++ IE+ L +VQ + LL S+HLL+AGGKR+RPVFVL+ KFG YD+ + Sbjct: 6 MYSFLDSDINIIEQSLEETVQAESPLLRNASMHLLQAGGKRIRPVFVLLSAKFGSYDISE 65 Query: 61 LKRVAIPLELIHSASLVHDDVIDDAELRRGELTVKAKWGDKIAMYTGDYIYAKALVMTSE 120 +K+VA+ LELIH ASLVHDDVIDDAELRRG+ T+KAKW ++ AMYTGDYI+AK+L + +E Sbjct: 66 IKKVAVALELIHMASLVHDDVIDDAELRRGKPTIKAKWDNRTAMYTGDYIFAKSLEVMTE 125 Query: 121 LGNPRIHQILSKAMVEMSIGEMEQIRDFFNSEQSVRHYLRRIRRKTALLIAISCQLGALA 180 + P H+IL+K +VE+ +GE+EQI+D + +Q++R YL RI+RKTALLIA SCQLGA+A Sbjct: 126 IERPEAHEILAKTIVEVCVGEIEQIKDKYRFDQNLRDYLLRIKRKTALLIAASCQLGAVA 185 Query: 181 ADAEPNTARLLYDYGYNVGMAFQIRDDLLDLSGTEKQIGKPPGSDMRQGNITLPVIYSLE 240 A + + + LY +GY VGM+FQI DD+LD +GTEK++GKP G D+ QGNITLPV++++E Sbjct: 186 AGVDESIHKKLYKFGYYVGMSFQITDDILDFTGTEKELGKPAGGDLLQGNITLPVLFAME 245 Query: 241 DQRLRSALLTELASIREG--NSGVGRAIDLILSGEGIARAEELASRYIAKALEALDQLPN 298 D+ +R E+ + E + + I LI + I R+ ++++Y++KAL LD LP Sbjct: 246 DEDIRR----EIVKVNETMDRKEIEKVISLIKNSGAIERSFTVSNQYLSKALAVLDTLPQ 301 Query: 299 NRTKRNLKDIAFFVTGRSY 317 NR K++L+DIA F+ R + Sbjct: 302 NRAKKSLRDIAKFIGKRKF 320 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.138 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,806,264,119 Number of extensions: 73775179 Number of successful extensions: 205393 Number of sequences better than 1.0e-05: 3670 Number of HSP's gapped: 199556 Number of HSP's successfully gapped: 3715 Length of query: 317 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 182 Effective length of database: 1,788,859,178 Effective search space: 325572370396 Effective search space used: 325572370396 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)