BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0256.0034.p (395 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KLR8.1 B3KLR8_9BACI Chorismate synthase EC=4.2.3.5 621 e-176 C0ZCD8.1 AROC_BREBN Chorismate synthase EC=4.2.3.5 467 e-129 Q5WGS8.1 AROC_BACSK Chorismate synthase EC=4.2.3.5 456 e-126 A8VXV0.1 A8VXV0_9BACI Chorismate synthase EC=4.2.3.5 430 e-118 A3F3T6.1 A3F3T6_BACSU Chorismate synthase EC=4.2.3.5 425 e-117 >B3KLR8.1 B3KLR8_9BACI Chorismate synthase EC=4.2.3.5 Length = 389 Score = 621 bits (1602), Expect = e-176, Method: Compositional matrix adjust. Identities = 299/389 (76%), Positives = 331/389 (85%) Query: 7 MSLRYLTAGETHGPQLTAIIEGLPSNLTLDFEELNFXXXXXXXXXXXXXXMQIEKDSAEI 66 MSLR+LTAGETHGPQLT IIEGLPSNL LDF+ LNF MQIEKD+A+I Sbjct: 1 MSLRFLTAGETHGPQLTTIIEGLPSNLELDFDALNFQLHRRQKGYGRGRRMQIEKDTAQI 60 Query: 67 VGGVRHGYTTGAPVALVVENKDWTHWKNIMNIEPIPGSDEEKRRVNRPRPGHADLNGGLK 126 +GGVRHGYTTGAP+ALVV+N DW HW+NIMN+EPI G+DE KRRV+RPRPGHADLNGGLK Sbjct: 61 LGGVRHGYTTGAPLALVVQNNDWKHWQNIMNVEPIEGNDEAKRRVHRPRPGHADLNGGLK 120 Query: 127 YNLTDLRNVLERSSXXXXXXXXXXXXXXXXXXXXFGVKIAGQVIRIGEIEAPANDLPVDE 186 YNL DLRNVLERSS FGVK+AGQVIRIGEIEAP ++L +DE Sbjct: 121 YNLKDLRNVLERSSARETTVRVAVGAIARQFLAQFGVKVAGQVIRIGEIEAPPHNLSIDE 180 Query: 187 LIEQTEASSVRVVDKETEKKMEAYIDKIKEEGDSIGGIVECIVEGLPVGLGSHVQSDRKL 246 LIE+TEASSVRVVD+ETEKKMEAYID+IK+EGDSIGGIVECI+EGLPVGLGSHVQ DRKL Sbjct: 181 LIERTEASSVRVVDEETEKKMEAYIDEIKKEGDSIGGIVECIIEGLPVGLGSHVQYDRKL 240 Query: 247 DGAIAGAVMSINAFKGVEIGIGFEAGKLRGSQVHDEIMYEASRGYYRASNRLGGFEGGMT 306 DG IA VMSINAFKGVE+GIGFEAG+LRGSQVHDEIMY RGY+RA+NRLGGFEGGMT Sbjct: 241 DGRIAQGVMSINAFKGVEVGIGFEAGELRGSQVHDEIMYSEERGYHRATNRLGGFEGGMT 300 Query: 307 NGMPVVVRGVMKPIPTLYKPLQSVDIDTKEPFTAQVERSDACAVPAACVVLENVVAWEIA 366 NGMP+VVRGVMKPIPTLYKPLQSVDIDTKE FTAQVERSDACAVPAA VV+E+VV WE+A Sbjct: 301 NGMPIVVRGVMKPIPTLYKPLQSVDIDTKEAFTAQVERSDACAVPAASVVMEHVVTWEVA 360 Query: 367 KAFMDKFGGDSIEEIRANYNNYQAQLESY 395 KAF++KFGGDS+EEI ANY NY QLE+Y Sbjct: 361 KAFLEKFGGDSMEEIHANYQNYLKQLENY 389 >C0ZCD8.1 AROC_BREBN Chorismate synthase EC=4.2.3.5 Length = 387 Score = 467 bits (1201), Expect = e-129, Method: Compositional matrix adjust. Identities = 237/387 (61%), Positives = 284/387 (73%) Query: 9 LRYLTAGETHGPQLTAIIEGLPSNLTLDFEELNFXXXXXXXXXXXXXXMQIEKDSAEIVG 68 +RYLTAGE+HGPQLTAIIEG+PSNL + EE+N MQIEKD +I+ Sbjct: 1 MRYLTAGESHGPQLTAIIEGVPSNLPISIEEINEQLARRQKGHGRGRRMQIEKDQVKILS 60 Query: 69 GVRHGYTTGAPVALVVENKDWTHWKNIMNIEPIPGSDEEKRRVNRPRPGHADLNGGLKYN 128 GVRHGYTTGAP+ LVVENKDWTHW+ IM+ EP+ EEKRRV+RPRPGHADLNG +KY+ Sbjct: 61 GVRHGYTTGAPITLVVENKDWTHWQGIMSAEPVEEGSEEKRRVSRPRPGHADLNGAIKYH 120 Query: 129 LTDLRNVLERSSXXXXXXXXXXXXXXXXXXXXFGVKIAGQVIRIGEIEAPANDLPVDELI 188 D+RN+LERSS FG++I GQV++I EI A ++ +DELI Sbjct: 121 QRDMRNILERSSARETTVRVAVGAVARQLLAQFGIRIGGQVLQINEIVAKRQEVSLDELI 180 Query: 189 EQTEASSVRVVDKETEKKMEAYIDKIKEEGDSIGGIVECIVEGLPVGLGSHVQSDRKLDG 248 +TE S VR +DKE E M A IDK KE+GDS+GG VE IVEG+P+GLGSHVQ DRKLDG Sbjct: 181 ARTEESPVRCLDKEAEPLMMAAIDKAKEDGDSLGGTVEVIVEGVPIGLGSHVQWDRKLDG 240 Query: 249 AIAGAVMSINAFKGVEIGIGFEAGKLRGSQVHDEIMYEASRGYYRASNRLGGFEGGMTNG 308 +A A+MSI AFKGVEIGIGFEA L+GSQVHDEI++ GY R +NR GG EGGMT G Sbjct: 241 RLAQAIMSIQAFKGVEIGIGFEAAGLKGSQVHDEIVWNEETGYSRKTNRAGGLEGGMTTG 300 Query: 309 MPVVVRGVMKPIPTLYKPLQSVDIDTKEPFTAQVERSDACAVPAACVVLENVVAWEIAKA 368 MPVVVRGVMKPIPTLYKPL SVDID++EPF+A +ERSD+CAVPAA VV E VVAWEIA A Sbjct: 301 MPVVVRGVMKPIPTLYKPLMSVDIDSREPFSASIERSDSCAVPAASVVAEAVVAWEIANA 360 Query: 369 FMDKFGGDSIEEIRANYNNYQAQLESY 395 +KF DS++++ N Y+A E + Sbjct: 361 MCEKFPSDSLDDMEENVRQYRAYTEKF 387 >Q5WGS8.1 AROC_BACSK Chorismate synthase EC=4.2.3.5 Length = 390 Score = 456 bits (1174), Expect = e-126, Method: Compositional matrix adjust. Identities = 229/390 (58%), Positives = 274/390 (70%), Gaps = 3/390 (0%) Query: 9 LRYLTAGETHGPQLTAIIEGLPSNLTLDFEELNFXXXXXXXXXXXXXXMQIEKDSAEIVG 68 +R+LTAGE+HGPQLTAIIEG+P+ L L E+++ MQIEKD +IV Sbjct: 1 MRFLTAGESHGPQLTAIIEGVPAQLPLLAEDIDRELARRQGGYGRGRRMQIEKDRVQIVS 60 Query: 69 GVRHGYTTGAPVALVVENKDWTHWKNIMNIEPIPGSDEEK--RRVNRPRPGHADLNGGLK 126 GVRHG TTGAP+ VVENKDW +W IM EPI + EK R++ RPRPGHADLNG +K Sbjct: 61 GVRHGMTTGAPITFVVENKDWKNWTKIMGAEPISEEEAEKMRRKLTRPRPGHADLNGAIK 120 Query: 127 YNLTDLRNVLERSSXXXXXXXXXXXXXXXXXXXXFGVKIAGQVIRIGEIEAP-ANDLPVD 185 Y D+RNVLERSS G+++AG V IG I A N + Sbjct: 121 YGHRDMRNVLERSSARETTVRVACGALAKVILKACGIEVAGHVREIGGIRAEETNYATIQ 180 Query: 186 ELIEQTEASSVRVVDKETEKKMEAYIDKIKEEGDSIGGIVECIVEGLPVGLGSHVQSDRK 245 EL +++E S VR +D++ KKM A IDK K++GDSIGGIVE +V G+P+GLGSHV DRK Sbjct: 181 ELQKRSEESPVRCLDEDASKKMMAAIDKAKQDGDSIGGIVEVVVAGVPIGLGSHVHYDRK 240 Query: 246 LDGAIAGAVMSINAFKGVEIGIGFEAGKLRGSQVHDEIMYEASRGYYRASNRLGGFEGGM 305 LDG IAGAVMSINAFKGVEIGIGFEA +L GSQVHDEI + GY R +NRLGGFEGGM Sbjct: 241 LDGKIAGAVMSINAFKGVEIGIGFEAARLPGSQVHDEITWSEEEGYKRKTNRLGGFEGGM 300 Query: 306 TNGMPVVVRGVMKPIPTLYKPLQSVDIDTKEPFTAQVERSDACAVPAACVVLENVVAWEI 365 TNGMP+VV+GVMKPIPTLYKPLQSVDID+KEPF A VERSD+CAVPAA VV ENVVAWE+ Sbjct: 301 TNGMPIVVKGVMKPIPTLYKPLQSVDIDSKEPFAAGVERSDSCAVPAASVVCENVVAWEV 360 Query: 366 AKAFMDKFGGDSIEEIRANYNNYQAQLESY 395 A A +D FG D + I+A + A + + Sbjct: 361 ANALLDTFGSDQLPLIQAAVERHHAASKEF 390 >A8VXV0.1 A8VXV0_9BACI Chorismate synthase EC=4.2.3.5 Length = 390 Score = 430 bits (1105), Expect = e-118, Method: Compositional matrix adjust. Identities = 228/390 (58%), Positives = 278/390 (71%), Gaps = 3/390 (0%) Query: 9 LRYLTAGETHGPQLTAIIEGLPSNLTLDFEELNFXXXXXXXXXXXXXXMQIEKDSAEIVG 68 +R+LTAGE+HGP+LTAIIEG+PS L+L E++N MQIEKD I Sbjct: 1 MRFLTAGESHGPELTAIIEGVPSQLSLTAEDINKHLARRQKGYGRGRRMQIEKDQVRITS 60 Query: 69 GVRHGYTTGAPVALVVENKDWTHWKNIMNIEPIPGSDEE--KRRVNRPRPGHADLNGGLK 126 GVRHG TTGAP+ LVVENKDWTHW IM EP+ +EE KR+++RPRPGHADLNG LK Sbjct: 61 GVRHGKTTGAPITLVVENKDWTHWTKIMGPEPLEPGEEEEVKRKISRPRPGHADLNGALK 120 Query: 127 YNLTDLRNVLERSSXXXXXXXXXXXXXXXXXXXXFGVKIAGQVIRIGEIEAPAND-LPVD 185 Y D+RNVLERSS FG+KIAG V+ IG++ A N+ L V+ Sbjct: 121 YRHRDMRNVLERSSARETTVRVAVGAVAQRILEEFGMKIAGHVLEIGDVVAEKNESLTVE 180 Query: 186 ELIEQTEASSVRVVDKETEKKMEAYIDKIKEEGDSIGGIVECIVEGLPVGLGSHVQSDRK 245 ++I +EAS VR D E+KM+ ID K+ GDSIGGIVE + E +PVGLGS VQ DRK Sbjct: 181 DIIALSEASEVRCTDPNAEEKMKQAIDDAKQNGDSIGGIVEVVAENVPVGLGSFVQYDRK 240 Query: 246 LDGAIAGAVMSINAFKGVEIGIGFEAGKLRGSQVHDEIMYEASRGYYRASNRLGGFEGGM 305 LD I+ +VMSINAFKGVE+G+GFEA + GS+VHDEI+Y GY R +N LGGFEGGM Sbjct: 241 LDAKISMSVMSINAFKGVEVGLGFEAARRNGSKVHDEIIYSEGTGYQRRTNNLGGFEGGM 300 Query: 306 TNGMPVVVRGVMKPIPTLYKPLQSVDIDTKEPFTAQVERSDACAVPAACVVLENVVAWEI 365 T GMPVVV+G MKPIPTLYKPLQSVDI+TKEPF+A +ERSD+CAVPAA VV E VAWEI Sbjct: 301 TTGMPVVVKGAMKPIPTLYKPLQSVDIETKEPFSASIERSDSCAVPAAAVVCEAAVAWEI 360 Query: 366 AKAFMDKFGGDSIEEIRANYNNYQAQLESY 395 A AF+DKFG D+I +I A+ +YQ ++ + Sbjct: 361 ADAFLDKFGSDTIPDIHAHLEHYQNEVRDF 390 >A3F3T6.1 A3F3T6_BACSU Chorismate synthase EC=4.2.3.5 Length = 390 Score = 425 bits (1092), Expect = e-117, Method: Compositional matrix adjust. Identities = 221/384 (57%), Positives = 272/384 (70%), Gaps = 3/384 (0%) Query: 9 LRYLTAGETHGPQLTAIIEGLPSNLTLDFEELNFXXXXXXXXXXXXXXMQIEKDSAEIVG 68 +RYLTAGE+HGPQLT IIEG+P+ L + E++NF MQIEKD A+I+ Sbjct: 1 MRYLTAGESHGPQLTTIIEGVPAGLYITEEDINFELARRQKGHGRGRRMQIEKDQAKIMS 60 Query: 69 GVRHGYTTGAPVALVVENKDWTHWKNIMNIEPIPGSDEE--KRRVNRPRPGHADLNGGLK 126 GVRH T G+P+ALVVEN DW HW IM PI +E+ KR+++RPRPGHADLNG +K Sbjct: 61 GVRHARTLGSPIALVVENNDWKHWTKIMGAAPITEDEEKEMKRQISRPRPGHADLNGAIK 120 Query: 127 YNLTDLRNVLERSSXXXXXXXXXXXXXXXXXXXXFGVKIAGQVIRIGEIEAPANDL-PVD 185 YN D+RNVLERSS G+K+AG V++IG ++A + +D Sbjct: 121 YNHRDMRNVLERSSARETTVRVAAGAVAKKILSELGIKVAGHVLQIGAVKAEKTEYTSID 180 Query: 186 ELIEQTEASSVRVVDKETEKKMEAYIDKIKEEGDSIGGIVECIVEGLPVGLGSHVQSDRK 245 +L TE S VR D+E KKM A ID+ K GDSIGGIVE IVEG+PVG+GS+V DRK Sbjct: 181 DLQRVTEESPVRCYDEEAGKKMMAAIDEAKANGDSIGGIVEVIVEGMPVGVGSYVHYDRK 240 Query: 246 LDGAIAGAVMSINAFKGVEIGIGFEAGKLRGSQVHDEIMYEASRGYYRASNRLGGFEGGM 305 LD +A AV+SINAFKGVE GIGFEA GS+VHDEI+++ +GY RA+NRLGG EGGM Sbjct: 241 LDSKLAAAVLSINAFKGVEFGIGFEAAGRNGSEVHDEIIWDEEKGYTRATNRLGGLEGGM 300 Query: 306 TNGMPVVVRGVMKPIPTLYKPLQSVDIDTKEPFTAQVERSDACAVPAACVVLENVVAWEI 365 T GMP+VVRGVMKPIPTLYKPL+SVDI+TKEPF+A +ERSD+CAVPAA VV E VAWEI Sbjct: 301 TTGMPIVVRGVMKPIPTLYKPLKSVDIETKEPFSASIERSDSCAVPAASVVAEAAVAWEI 360 Query: 366 AKAFMDKFGGDSIEEIRANYNNYQ 389 A A +++FG D I+ IR N N + Sbjct: 361 ANAVVEQFGLDQIDRIRENVENMR 384 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.315 0.135 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,202,365,873 Number of extensions: 89096760 Number of successful extensions: 180649 Number of sequences better than 1.0e-05: 1639 Number of HSP's gapped: 173029 Number of HSP's successfully gapped: 1691 Length of query: 395 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 257 Effective length of database: 1,760,596,133 Effective search space: 452473206181 Effective search space used: 452473206181 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 132 (55.5 bits)