BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0256.0033.p (368 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KLR9.1 B3KLR9_9BACI 3-dehydroquinate synthase EC=4.2.3.4 483 e-134 B1DMG9.1 B1DMG9_9BACL 3-dehydroquinate synthase 425 e-117 C0ZCD9.1 C0ZCD9_BREBN 3-dehydroquinate synthase EC=4.2.3.4 336 2e-90 A4J3A3.1 A4J3A3_DESRM 3-dehydroquinate synthase EC=4.2.3.4 308 8e-82 C4LB07.1 C4LB07_TOLAT 3-dehydroquinate synthase 287 1e-75 >B3KLR9.1 B3KLR9_9BACI 3-dehydroquinate synthase EC=4.2.3.4 Length = 364 Score = 483 bits (1243), Expect = e-134, Method: Compositional matrix adjust. Identities = 240/364 (65%), Positives = 285/364 (78%) Query: 2 MRSITVELGERSYPIHIGSGLLRDIGQHCVKAGFPLRSPLLIVSDSEVAPRYLPVVEAAL 61 MR++ VELGERSYPI+IG+G+L +G+ K G +SP LIV+D +VAP YL VE L Sbjct: 1 MRTLHVELGERSYPIYIGAGILNTLGELSAKHGITRKSPHLIVTDDQVAPHYLAQVEEQL 60 Query: 62 RGEGYAVVSHVIPAGEASKSLAVYEEVITTAIQAGLDRSSXXXXXXXXXXXXXXXXXXXT 121 + EGY V+SH++P+GE+SKSL VYEEV+TTAI LDRSS T Sbjct: 61 KSEGYTVISHIVPSGESSKSLQVYEEVMTTAINGKLDRSSAVFALGGGVVGDLAGFVAAT 120 Query: 122 FMRGIGFIQIPTTILAHDSSVGGKVAVNHPLAKNMIGAFYQPSLVLYDLDTLSTLPPRQI 181 +MRG+ F+QIPTTILAHDSSVGGKVAVNHPLAKNMIGAF+QP +V+YD++TL +LP R++ Sbjct: 121 YMRGVTFVQIPTTILAHDSSVGGKVAVNHPLAKNMIGAFHQPVMVVYDVNTLMSLPAREV 180 Query: 182 SSGLAEVVKHGLILDRDFAYWCLEHASELLSLNTEALGYALERGCAIKAQVVSNDEREHG 241 SSGLAE+VKHGLILDRDFAYWC E+ S+LLSL EAL Y LERGCAIKA VVS DERE G Sbjct: 181 SSGLAEMVKHGLILDRDFAYWCRENGSKLLSLEPEALIYGLERGCAIKADVVSQDEREGG 240 Query: 242 QRAFLNLGHTIGHAIEAVGGYGVFLHGEAIAIGMAGSALLAAKLGRERAIYEDTIAMLSA 301 RA LNLGHTIGHAIEAVGGYG FLHGEAIAIGMAGSA LA KLGR ++E+T++ML A Sbjct: 241 LRAILNLGHTIGHAIEAVGGYGRFLHGEAIAIGMAGSARLAEKLGRPADLHEETVSMLKA 300 Query: 302 LSLPVKLPAEYSEDELMEAMMHDKKFKEGKMTFIVPDSIGAVSIVNDVQPSAVREVLAQL 361 ++LPV LP + S D +M+AM HDKKF EG+M FIVP+SIG+VSIV DV AVR V+ QL Sbjct: 301 VNLPVILPDDVSVDRIMDAMQHDKKFAEGQMVFIVPESIGSVSIVKDVPVEAVRSVVEQL 360 Query: 362 KKEG 365 K EG Sbjct: 361 KGEG 364 >B1DMG9.1 B1DMG9_9BACL 3-dehydroquinate synthase Length = 364 Score = 425 bits (1092), Expect = e-117, Method: Compositional matrix adjust. Identities = 209/363 (57%), Positives = 261/363 (71%) Query: 2 MRSITVELGERSYPIHIGSGLLRDIGQHCVKAGFPLRSPLLIVSDSEVAPRYLPVVEAAL 61 MR + VELGERSYPI+IG GLL++ + K L+SPLLI++D VAP YL +E L Sbjct: 1 MRELKVELGERSYPIYIGEGLLQEAAAYFKKHQISLKSPLLIITDERVAPHYLEPLEGTL 60 Query: 62 RGEGYAVVSHVIPAGEASKSLAVYEEVITTAIQAGLDRSSXXXXXXXXXXXXXXXXXXXT 121 R G+ V S V+PAGE+SKSL++ E ++T A++AGLDR S + Sbjct: 61 REAGFTVASAVVPAGESSKSLSMLESLVTKALEAGLDRKSTIVALGGGVVGDLAGFVAAS 120 Query: 122 FMRGIGFIQIPTTILAHDSSVGGKVAVNHPLAKNMIGAFYQPSLVLYDLDTLSTLPPRQI 181 +MRG+ F+QIPTTILAHDSSVGGKVAVNHPLAKN+IGAF+QP +VLYDL+TL +LPPR++ Sbjct: 121 YMRGVKFVQIPTTILAHDSSVGGKVAVNHPLAKNIIGAFHQPEMVLYDLNTLQSLPPREV 180 Query: 182 SSGLAEVVKHGLILDRDFAYWCLEHASELLSLNTEALGYALERGCAIKAQVVSNDEREHG 241 S+GL+EVVKHGLI D +F WC E+A LL+L +ALGYAL +GC++KA VVS DERE+ Sbjct: 181 SAGLSEVVKHGLIWDAEFTTWCEENADRLLALEPDALGYALYKGCSVKAAVVSQDEREND 240 Query: 242 QRAFLNLGHTIGHAIEAVGGYGVFLHGEAIAIGMAGSALLAAKLGRERAIYEDTIAMLSA 301 RA LNLGHTIGHA+EAV GYG LHGEAI+IGM GSA L + G +Y T +LS Sbjct: 241 LRAILNLGHTIGHALEAVAGYGELLHGEAISIGMIGSARLGLRYGAPEEVYTTTKRVLSK 300 Query: 302 LSLPVKLPAEYSEDELMEAMMHDKKFKEGKMTFIVPDSIGAVSIVNDVQPSAVREVLAQL 361 + LPVKLPA + D +M+AMMHDKKF E M F+VP IG V I DVQ + VR ++ QL Sbjct: 301 VGLPVKLPAHFDVDAIMDAMMHDKKFSESTMVFVVPTEIGKVEIKKDVQSAWVRYIVEQL 360 Query: 362 KKE 364 K E Sbjct: 361 KLE 363 >C0ZCD9.1 C0ZCD9_BREBN 3-dehydroquinate synthase EC=4.2.3.4 Length = 366 Score = 336 bits (861), Expect = 2e-90, Method: Compositional matrix adjust. Identities = 176/360 (48%), Positives = 227/360 (63%), Gaps = 1/360 (0%) Query: 2 MRSITVELGERSYPIHIGSGLLRDIGQHCVKAGFPLRSPLLIVSDSEVAPRYLPVVEAAL 61 + +TVELGERSY I IG GLL V+AG S L+IV+D VA YL + L Sbjct: 3 VEKLTVELGERSYEIVIGEGLLDQAAALLVEAGIASTSKLMIVTDENVAVHYLEPLLGVL 62 Query: 62 RGEGYAVVSHVIPAGEASKSLAVYEEVITTAIQAGLDRSSXXXXXXXXXXXXXXXXXXXT 121 R GY S VI AGE SKSLAVYE ++T AI AGLDR S T Sbjct: 63 RQHGYQAHSAVIAAGEQSKSLAVYERLMTEAIDAGLDRKSAVLALGGGVVGDLAGFVAAT 122 Query: 122 FMRGIGFIQIPTTILAHDSSVGGKVAVNHPLAKNMIGAFYQPSLVLYDLDTLSTLPPRQI 181 +MRGI F+Q+PTT+LAHDSSVGGKVA+NHPL KN+IGAF+QP +V+YD L +LP R++ Sbjct: 123 YMRGIDFVQMPTTLLAHDSSVGGKVAINHPLGKNLIGAFHQPKVVIYDTIALHSLPKREV 182 Query: 182 SSGLAEVVKHGLILDRDFAYWCLEHASELLSLNTEALGYALERGCAIKAQVVSNDEREHG 241 ++G AEVVKHGLI D F W ++A L L++E LG A+E+GCA+KA +VS DE E G Sbjct: 183 AAGFAEVVKHGLIADAAFVDWLEDNADRLWQLDSELLGKAIEKGCAVKAAIVSQDETEQG 242 Query: 242 QRAFLNLGHTIGHAIEAVGGYGVFLHGEAIAIGMAGSALLAAKLG-RERAIYEDTIAMLS 300 QRA LNLGHT GHA EA+ Y HGEAI+IGM +A +A ++G E +Y T ML Sbjct: 243 QRALLNLGHTFGHAFEALSAYSTLNHGEAISIGMCLAAKVAERIGFAETGVYHRTKRMLE 302 Query: 301 ALSLPVKLPAEYSEDELMEAMMHDKKFKEGKMTFIVPDSIGAVSIVNDVQPSAVREVLAQ 360 LP P S + ++EAM DKK GK+ ++P +IG V +V +++ + V+ + Sbjct: 303 LFHLPTAWPGNLSPEAVLEAMKRDKKTVGGKLALVLPRAIGQVEVVKNIEEELILGVMRE 362 >A4J3A3.1 A4J3A3_DESRM 3-dehydroquinate synthase EC=4.2.3.4 Length = 359 Score = 308 bits (788), Expect = 8e-82, Method: Compositional matrix adjust. Identities = 166/361 (45%), Positives = 224/361 (62%), Gaps = 4/361 (1%) Query: 2 MRSITVELGERSYPIHIGSGLLRDIGQHCVKAGFPLRSPLLIVSDSEVAPRYLPVVEAAL 61 M +I V+LG RSYPI+I S +L IG + + PL +L+V+D +VAP Y P V +L Sbjct: 1 MGTIQVDLGNRSYPIYIASAILDKIGTYLSEQ--PLGKKVLLVTDQQVAPLYAPRVIESL 58 Query: 62 RGEGYAVVSHVIPAGEASKSLAVYEEVITTAIQAGLDRSSXXXXXXXXXXXXXXXXXXXT 121 + G+ V IPAGE SK+L + A LDR S T Sbjct: 59 KNAGFKVAVAEIPAGEPSKTLEQASRLYDLAFDHALDRQSSVIALGGGVVGDLAGFVAAT 118 Query: 122 FMRGIGFIQIPTTILAH-DSSVGGKVAVNHPLAKNMIGAFYQPSLVLYDLDTLSTLPPRQ 180 +MRG+ FIQIPTT+LA DSSVGGKVAVNH KNMIGAFYQP +V+ D+ TL TLP R+ Sbjct: 119 YMRGVPFIQIPTTLLAQVDSSVGGKVAVNHSRGKNMIGAFYQPQMVMIDVATLQTLPERE 178 Query: 181 ISSGLAEVVKHGLILDRDFAYWCLEHASELLSLNTEALGYALERGCAIKAQVVSNDEREH 240 + +GLAEV+K+G+I D F W ++ S +L+L+T AL E C IKA VV DERE Sbjct: 179 LKAGLAEVIKYGVIWDGSFFTWLEKNYSRILNLDTTALEQVAETCCKIKASVVEQDEREQ 238 Query: 241 GQRAFLNLGHTIGHAIEAVGGYGVFLHGEAIAIGMAGSALLAAKLG-RERAIYEDTIAML 299 G RA LN GHT+GHAIE++ GYG +LHGEA+A+GM +A LA G + +E ++ Sbjct: 239 GCRAILNYGHTVGHAIESLSGYGTYLHGEAVAMGMISAARLALNEGILSQQDFERIYRLI 298 Query: 300 SALSLPVKLPAEYSEDELMEAMMHDKKFKEGKMTFIVPDSIGAVSIVNDVQPSAVREVLA 359 SA+ LP +LP +++++M HDKK GK+ F++P S+G V I DV+ + + VL+ Sbjct: 299 SAVGLPTELPRGLGPQDIIDSMYHDKKTVLGKLVFVLPRSLGQVDIFKDVKEADILAVLS 358 Query: 360 Q 360 Q Sbjct: 359 Q 359 >C4LB07.1 C4LB07_TOLAT 3-dehydroquinate synthase Length = 358 Score = 287 bits (735), Expect = 1e-75, Method: Compositional matrix adjust. Identities = 156/363 (42%), Positives = 224/363 (61%), Gaps = 15/363 (4%) Query: 2 MRSITVELGERSYPIHIGSGLLRDIGQHCVKAGFPLRSPLLIVSDSEVAPRYLPVVEAAL 61 M ++TV L ERSYPI++G+GLL + A ++IV++ VAP YL V+ L Sbjct: 1 METLTVNLAERSYPIYVGAGLLT---SSALFAPHIAGQRVMIVTNDTVAPLYLDVLRKTL 57 Query: 62 RGEGYAVVSHVIPAGEASKSLAVYEEVITTAIQAGLDRSSXXXXXXXXXXXXXXXXXXXT 121 GY + + ++P GEA K+LA +E +++ ++ R + Sbjct: 58 --AGYQIDTLILPDGEAHKTLASFEVIMSALLKGTHGRDTTLIALGGGVIGDLVGFAAAC 115 Query: 122 FMRGIGFIQIPTTILAH-DSSVGGKVAVNHPLAKNMIGAFYQPSLVLYDLDTLSTLPPRQ 180 + RG+ FIQ+PTT+L+ DSSVGGK AVNHPL KNMIGAFYQP V+ D+D L TLP R+ Sbjct: 116 YQRGVPFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQPKAVIIDIDCLRTLPSRE 175 Query: 181 ISSGLAEVVKHGLILDRDFAYWCLEHASELLSLNTEALGYALERGCAIKAQVVSNDEREH 240 +++G+AEV+K+G+I D DF W +H S++ SL+ +AL + R CAIKA VV+ DE E+ Sbjct: 176 LAAGMAEVIKYGIIWDADFFSWLEQHISDIQSLSPDALSKIILRCCAIKADVVAQDETEN 235 Query: 241 GQRAFLNLGHTIGHAIEAVGGYGVFLHGEAIAIGMAGSALLAAKLGRERAIYEDT----- 295 G RA LNLGHT GHAIEA GYG +LHGEA+A G +A + +LG + DT Sbjct: 236 GVRALLNLGHTFGHAIEAEQGYGKWLHGEAVAAGTVQAAETSLRLG----LLSDTDVSRI 291 Query: 296 IAMLSALSLPVKLPAEYSEDELMEAMMHDKKFKEGKMTFIVPDSIGAVSIVNDVQPSAVR 355 A+L A +LPV PA+ + D+ + M+ DKK K GK+ ++P +IG + DV+ + +R Sbjct: 292 KALLKAANLPVTAPADMTYDDYIHHMLRDKKAKAGKLRLVLPQAIGRADLFTDVEQTVLR 351 Query: 356 EVL 358 V+ Sbjct: 352 AVI 354 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.136 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,922,998,935 Number of extensions: 74455496 Number of successful extensions: 207665 Number of sequences better than 1.0e-05: 1664 Number of HSP's gapped: 201667 Number of HSP's successfully gapped: 1676 Length of query: 368 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 231 Effective length of database: 1,770,017,148 Effective search space: 408873961188 Effective search space used: 408873961188 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)