BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0256.0031.p (509 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KLS1.1 B3KLS1_9BACI Anthranilate synthase component I EC=4.1.3.27 813 0.0 B1DMG7.1 B1DMG7_9BACL Anthranilate synthase component I EC=4.1.3.27 690 0.0 C0ZCE1.1 C0ZCE1_BREBN Anthranilate synthase component I EC=4.1.3.27 510 e-142 A3DDT0.1 A3DDT0_CLOTH Anthranilate synthase, component I EC=4.1.... 483 e-134 P14953.1 TRPE_CLOTM Anthranilate synthase component 1 EC=4.1.3.27 483 e-134 >B3KLS1.1 B3KLS1_9BACI Anthranilate synthase component I EC=4.1.3.27 Length = 517 Score = 813 bits (2101), Expect = 0.0, Method: Compositional matrix adjust. Identities = 385/509 (75%), Positives = 434/509 (85%) Query: 1 VLSLSREYNLIPVVTRLLADMETPIRLFQRFAGQDRAFLLESVEGGIQWARYSFIGSDPF 60 V++++ EYNLIPV RLLADMETPIR+FQR+A + RAFLLESVEGG+QWARYSFIG+DPF Sbjct: 9 VVAMAGEYNLIPVAIRLLADMETPIRIFQRYAERKRAFLLESVEGGVQWARYSFIGTDPF 68 Query: 61 LMVSXXXXXXXXXXXXXXRRLSGKPVEELKALLRSYRSPKLDGMPPFTGGAIGFFGYDLL 120 LM++ L GKPVEELKALLR+YRSP+L MPPFTGGAIGFFGYDLL Sbjct: 69 LMITGKKGKIEIEKNGERSLLEGKPVEELKALLRTYRSPQLKEMPPFTGGAIGFFGYDLL 128 Query: 121 QYYEKLSAHAVDDLNMDDIRFMFCDRIIVFDHVKQQILLVGNLHIKDGDTDSDIRAGYEE 180 QYYEKL AHA+DDL MDDIRFMFCD++IVFDHVKQQI+LVGN+H+K GD+D IR Y + Sbjct: 129 QYYEKLPAHAMDDLGMDDIRFMFCDQVIVFDHVKQQIMLVGNVHVKPGDSDDTIRQTYMD 188 Query: 181 LSRKLENMAEELQKEGPKENVNRRSIPQDIELGEIHSNLTREQYISNVEQAKEYIRAGDI 240 + R LE AE+LQ +GP+ENVN R IP D+++G+I SN+T+E+Y+SNVEQAKEYIRAGDI Sbjct: 189 VCRTLEAAAEQLQDQGPRENVNSRGIPADVDMGDIKSNMTKEKYLSNVEQAKEYIRAGDI 248 Query: 241 FQVVLSQRLHIETEVSPLHVYRMLRTLNPSPYMYYLKMDEEIIVGTSPEALVKVDGGRVE 300 FQVVLSQR HIETEVSPLHVYR+LRTLNPSPYMYYLKMD++IIVGTSPEALVKVDG R+E Sbjct: 249 FQVVLSQRFHIETEVSPLHVYRVLRTLNPSPYMYYLKMDDDIIVGTSPEALVKVDGRRLE 308 Query: 301 TRPIAGTRPRGENXXXXXXXXXXXXXXXXXXXXHLMLVDLGRNDLGRVSKFGTVKCDSFM 360 TRPIAGTRPRG N HLMLVDLGRNDLGRVS+FGTVKCD+FM Sbjct: 309 TRPIAGTRPRGSNEAEDQALADDLLQDEKERAEHLMLVDLGRNDLGRVSEFGTVKCDTFM 368 Query: 361 EIERYSHVMHIVSNVSGTLDEGKDFFDAFLSCLPAGTVSGAPKLRAMEIIAELEREARGA 420 EIE+YSHVMH+VS+VSG L E KDFFDAFLSCLPAGTVSGAPKLRAMEIIAELEREARGA Sbjct: 369 EIEKYSHVMHMVSSVSGQLSENKDFFDAFLSCLPAGTVSGAPKLRAMEIIAELEREARGA 428 Query: 421 YAGAIGYLGFSGNMDSCITIRTIIFRKGRAYVQAGAGIVWDSVPEKEYEETVNKAKAMLK 480 YAGAIGYLGF GNMDSCITIRTIIFR+G+AYVQAGAGIVWDSVPEKEYEETVNKAK MLK Sbjct: 429 YAGAIGYLGFGGNMDSCITIRTIIFRQGKAYVQAGAGIVWDSVPEKEYEETVNKAKGMLK 488 Query: 481 AIRMAEAMFPAEVKEKQVINQDYMYEYTP 509 AIR+AEAMFP+ +INQDY+Y+YTP Sbjct: 489 AIRVAEAMFPSSTGANDLINQDYLYQYTP 517 >B1DMG7.1 B1DMG7_9BACL Anthranilate synthase component I EC=4.1.3.27 Length = 520 Score = 690 bits (1780), Expect = 0.0, Method: Compositional matrix adjust. Identities = 334/505 (66%), Positives = 392/505 (77%), Gaps = 2/505 (0%) Query: 1 VLSLSREYNLIPVVTRLLADMETPIRLFQRFAGQDRAFLLESVEGGIQWARYSFIGSDPF 60 V L+ +YN+IP+V +LAD ETPIRLFQ FA + AFLLESVEGG++WARYSFIG+DPF Sbjct: 8 VTKLADQYNIIPIVRYILADTETPIRLFQHFAKEQHAFLLESVEGGVKWARYSFIGTDPF 67 Query: 61 LMVSXXXXXXXXXXXXXXRRLSGKPVEELKALLRSYRSPKLDGMPPFTGGAIGFFGYDLL 120 +M+ + KP+E LKA LR +RSP L +PPFTGGAIG+FGYDLL Sbjct: 68 MMLYGKNGKMVLEHNGEKQEFDEKPIELLKAQLRGFRSPSLPELPPFTGGAIGYFGYDLL 127 Query: 121 QYYEKLSAHAVDDLNMDDIRFMFCDRIIVFDHVKQQILLVGNLHIKDGDTDSDIRAGYEE 180 QYYE L AH +DDL M+D++FMFCD++IVFDH KQQ+ ++GN+HI T++DI Y Sbjct: 128 QYYENLPAHRIDDLQMNDLQFMFCDQVIVFDHFKQQLQIIGNVHIPHAATNADIEKAYNR 187 Query: 181 LSRKLENMAEELQKEGPKENVNRRSIPQDIELGEIHSNLTREQYISNVEQAKEYIRAGDI 240 + K+E E LQ+ + SIP D ELG++ SNLT+EQ+ISNVEQAKEYIRAGDI Sbjct: 188 AAAKIEATIERLQQPITVPVTSGSSIPVDPELGDVQSNLTKEQFISNVEQAKEYIRAGDI 247 Query: 241 FQVVLSQRLHIETEVSPLHVYRMLRTLNPSPYMYYLKMDEEIIVGTSPEALVKVDGGR-V 299 FQVVLSQR IETEV PLHVYR+LRT+NPSPYMYYLKM +E+IVGTSPEALVKV+G R V Sbjct: 248 FQVVLSQRFSIETEVDPLHVYRVLRTMNPSPYMYYLKMGDEVIVGTSPEALVKVNGDRKV 307 Query: 300 ETRPIAGTRPRGENXXXXXXXXXXXXXXXXXXXXHLMLVDLGRNDLGRVSKFGTVKCDSF 359 ETRPIAGTRPRG+ HLMLVDLGRND+GRVS+FGTV+CD++ Sbjct: 308 ETRPIAGTRPRGKTPEEDKALEVELLADEKERAEHLMLVDLGRNDIGRVSEFGTVRCDAY 367 Query: 360 MEIERYSHVMHIVSNVSGTLDEGKDFFDAFLSCLPAGTVSGAPKLRAMEIIAELEREARG 419 MEIERYSHVMHIVSNVSG L E KDFFDAFLSCLPAGTVSGAPKLRAMEIIAELE EARG Sbjct: 368 MEIERYSHVMHIVSNVSGKLREDKDFFDAFLSCLPAGTVSGAPKLRAMEIIAELENEARG 427 Query: 420 AYAGAIGYLGFSGNMDSCITIRTIIFRKGRAYVQAGAGIVWDSVPEKEYEETVNKAKAML 479 AYAGAIGYLGF G++D+CITIRTI+F+ G+AYVQ+GAGIVWDSVPE EY ETVNKAKA L Sbjct: 428 AYAGAIGYLGFGGSLDTCITIRTIVFKNGKAYVQSGAGIVWDSVPESEYMETVNKAKACL 487 Query: 480 KAIRMAEAMF-PAEVKEKQVINQDY 503 KAIR AEA+F + +Q +N DY Sbjct: 488 KAIRAAEAVFGKRKSSAEQAVNADY 512 >C0ZCE1.1 C0ZCE1_BREBN Anthranilate synthase component I EC=4.1.3.27 Length = 504 Score = 510 bits (1313), Expect = e-142, Method: Compositional matrix adjust. Identities = 261/496 (52%), Positives = 337/496 (67%), Gaps = 5/496 (1%) Query: 1 VLSLSREYNLIPVVTRLLADMETPIRLFQRFAGQDRAFLLESVEGGIQWARYSFIGSDPF 60 V +LS Y+LIPV +LLAD ETPIRL+Q+ D +FLLESVEGG +WAR+SFIG +PF Sbjct: 9 VKTLSASYSLIPVSMKLLADQETPIRLYQKIRKSD-SFLLESVEGGARWARFSFIGMNPF 67 Query: 61 LMVSXXXXXXXXXXXXXXRRL-SGKPVEELKALLRSYRSPKLDGMPPFTGGAIGFFGYDL 119 +V + + +G PV L+ Y+SPKL G+P +GGA+GFFGY+ Sbjct: 68 QIVEAKGEEITVSYRTGEKHVHTGNPVSFLREETDRYKSPKLPGLPRLSGGAVGFFGYNT 127 Query: 120 LQYYEKLSAHAVDDLNMDDIRFMFCDRIIVFDHVKQQILLVGNLHIKDGDTDSDIRAGYE 179 L+Y+E L AH + + + D+RF+F D +I FDH+KQ+I L+ NLH++ GDT I Y+ Sbjct: 128 LRYFENLPAHRKEAVRVPDMRFLFVDEMIAFDHLKQEIQLIVNLHVEPGDTADTIGEKYK 187 Query: 180 ELSRKLENMAEELQKEGPKENVNRRSIPQDIELG-EIHSNLTREQYISNVEQAKEYIRAG 238 ++ ++E +A ++ P E R + D + N+TREQY V QAKEYI AG Sbjct: 188 QVCERIEELATKVM--APLEMDQRIQVAADTPAPLTVQPNMTREQYEQLVVQAKEYIAAG 245 Query: 239 DIFQVVLSQRLHIETEVSPLHVYRMLRTLNPSPYMYYLKMDEEIIVGTSPEALVKVDGGR 298 DIFQVVLSQR ++T+V P VYR+LRTLNPSPYMYYL+ + E +VGTSPE LV+V+ + Sbjct: 246 DIFQVVLSQRFSVKTDVDPFAVYRLLRTLNPSPYMYYLEYESETVVGTSPELLVRVEDEK 305 Query: 299 VETRPIAGTRPRGENXXXXXXXXXXXXXXXXXXXXHLMLVDLGRNDLGRVSKFGTVKCDS 358 VE RPIAGTR RG H ML+DLGRND+G+VS +G+VK + Sbjct: 306 VEMRPIAGTRKRGATPQEDAALAADLLADKKERAEHYMLLDLGRNDVGKVSTYGSVKVEE 365 Query: 359 FMEIERYSHVMHIVSNVSGTLDEGKDFFDAFLSCLPAGTVSGAPKLRAMEIIAELEREAR 418 + IE YSHVMH+VS+V+G L EG FDA LS PAGTVSG+PKLRAMEIIAELE +AR Sbjct: 366 ALVIENYSHVMHMVSHVTGKLREGLHAFDALLSAFPAGTVSGSPKLRAMEIIAELEPDAR 425 Query: 419 GAYAGAIGYLGFSGNMDSCITIRTIIFRKGRAYVQAGAGIVWDSVPEKEYEETVNKAKAM 478 YAGAIGY+ F G++DSCITIRT+ F+ G A+VQAGAGIV DSVP EY+ETVNKA AM Sbjct: 426 HLYAGAIGYISFDGSLDSCITIRTLFFQDGYAHVQAGAGIVADSVPASEYQETVNKAAAM 485 Query: 479 LKAIRMAEAMFPAEVK 494 L A+ AE +F +V+ Sbjct: 486 LSALEKAEQLFVRKVE 501 >A3DDT0.1 A3DDT0_CLOTH Anthranilate synthase, component I EC=4.1.3.27 Length = 494 Score = 483 bits (1242), Expect = e-134, Method: Compositional matrix adjust. Identities = 247/485 (50%), Positives = 321/485 (66%), Gaps = 9/485 (1%) Query: 4 LSREYNLIPVVTRLLADMETPIRLFQRFAGQDRAFLLESVEGGIQWARYSFIGSDPFLMV 63 ++++YN+IPV + ADMETPI LF+RF FLLESVEGG +WARYS IG +PFL+V Sbjct: 12 MAKDYNIIPVTMEVYADMETPISLFKRFEESSCCFLLESVEGGEKWARYSIIGKNPFLVV 71 Query: 64 -SXXXXXXXXXXXXXXRRLSGKPVEELKALLRSYRSPKLDGMPPFTGGAIGFFGYDLLQY 122 S R + G PVE +K ++ ++ L +P F GGA+G+FGYDL+++ Sbjct: 72 ESYKNKTIIRERNGSQREVEGNPVEIIKGIMGKFKGANLPNLPRFNGGAVGYFGYDLIRH 131 Query: 123 YEKLSAHAVDDLNMDDIRFMFCDRIIVFDHVKQQILLVGNLHIKDGDTDSDIRAGYEELS 182 YE L DD+ + + FMF D ++V+DH+KQ+I ++ NLH+ + +I Y Sbjct: 132 YENLPNVPEDDMGLPECHFMFTDEVLVYDHLKQKIHIIVNLHV-----NGNIERAYISAV 186 Query: 183 RKLENMAEEL--QKEGPKENVNRRSIPQDIELGEIHSNLTREQYISNVEQAKEYIRAGDI 240 +++ + E+ + +N + EL + SN+++E + NV +AK+YIR GDI Sbjct: 187 DRIKTIHREILDTRWKTADNSVLSYNKKKNELA-VTSNISKEDFCRNVLKAKQYIRDGDI 245 Query: 241 FQVVLSQRLHIETEVSPLHVYRMLRTLNPSPYMYYLKMDEEIIVGTSPEALVKVDGGRVE 300 FQVVLSQRL +ET +P ++YR LR +NPSPYMYYLK I+G+SPE LV+V+ G VE Sbjct: 246 FQVVLSQRLCVETNENPFNIYRALRVINPSPYMYYLKFGGYRIIGSSPEMLVRVENGIVE 305 Query: 301 TRPIAGTRPRGENXXXXXXXXXXXXXXXXXXXXHLMLVDLGRNDLGRVSKFGTVKCDSFM 360 T PIAGTR RG H+MLVDLGRND+GRVSKFGTV + M Sbjct: 306 TCPIAGTRKRGRTKEEDEALEKELLSDEKEIAEHVMLVDLGRNDIGRVSKFGTVAVKNLM 365 Query: 361 EIERYSHVMHIVSNVSGTLDEGKDFFDAFLSCLPAGTVSGAPKLRAMEIIAELEREARGA 420 IERYSHVMH+V+NV G + E K FDA +S LPAGT+SGAPK+RAMEII ELE RG Sbjct: 366 HIERYSHVMHVVTNVQGEIREDKTPFDALMSILPAGTLSGAPKVRAMEIIDELETVKRGP 425 Query: 421 YAGAIGYLGFSGNMDSCITIRTIIFRKGRAYVQAGAGIVWDSVPEKEYEETVNKAKAMLK 480 Y GAIGYL F+GN+DSCITIRTII + G+AYVQAGAGIV DSVPE+EYEE NKA A+LK Sbjct: 426 YGGAIGYLSFNGNLDSCITIRTIILKDGKAYVQAGAGIVADSVPEREYEECYNKAMALLK 485 Query: 481 AIRMA 485 AI A Sbjct: 486 AIEEA 490 >P14953.1 TRPE_CLOTM Anthranilate synthase component 1 EC=4.1.3.27 Length = 494 Score = 483 bits (1242), Expect = e-134, Method: Compositional matrix adjust. Identities = 247/485 (50%), Positives = 321/485 (66%), Gaps = 9/485 (1%) Query: 4 LSREYNLIPVVTRLLADMETPIRLFQRFAGQDRAFLLESVEGGIQWARYSFIGSDPFLMV 63 ++++YN+IPV + ADMETPI LF+RF FLLESVEGG +WARYS IG +PFL+V Sbjct: 12 MAKDYNIIPVTMEVYADMETPISLFKRFEESSCCFLLESVEGGEKWARYSIIGKNPFLVV 71 Query: 64 -SXXXXXXXXXXXXXXRRLSGKPVEELKALLRSYRSPKLDGMPPFTGGAIGFFGYDLLQY 122 S R + G PVE +K ++ ++ L +P F GGA+G+FGYDL+++ Sbjct: 72 ESYKNKTIIRERNGSQREVEGNPVEIIKGIMGKFKGANLPNLPRFNGGAVGYFGYDLIRH 131 Query: 123 YEKLSAHAVDDLNMDDIRFMFCDRIIVFDHVKQQILLVGNLHIKDGDTDSDIRAGYEELS 182 YE L DD+ + + FMF D ++V+DH+KQ+I ++ NLH+ + +I Y Sbjct: 132 YENLPNVPEDDMGLPECHFMFTDEVLVYDHLKQKIHIIVNLHV-----NGNIERAYISAV 186 Query: 183 RKLENMAEEL--QKEGPKENVNRRSIPQDIELGEIHSNLTREQYISNVEQAKEYIRAGDI 240 +++ + E+ + +N + EL + SN+++E + NV +AK+YIR GDI Sbjct: 187 DRIKTIHREILDTRWKTADNSVLSYNKKKNELA-VTSNISKEDFCRNVLKAKQYIRDGDI 245 Query: 241 FQVVLSQRLHIETEVSPLHVYRMLRTLNPSPYMYYLKMDEEIIVGTSPEALVKVDGGRVE 300 FQVVLSQRL +ET +P ++YR LR +NPSPYMYYLK I+G+SPE LV+V+ G VE Sbjct: 246 FQVVLSQRLCVETNENPFNIYRALRVINPSPYMYYLKFGGYRIIGSSPEMLVRVENGIVE 305 Query: 301 TRPIAGTRPRGENXXXXXXXXXXXXXXXXXXXXHLMLVDLGRNDLGRVSKFGTVKCDSFM 360 T PIAGTR RG H+MLVDLGRND+GRVSKFGTV + M Sbjct: 306 TCPIAGTRKRGRTKEEDEALEKELLSDEKEIAEHVMLVDLGRNDIGRVSKFGTVAVKNLM 365 Query: 361 EIERYSHVMHIVSNVSGTLDEGKDFFDAFLSCLPAGTVSGAPKLRAMEIIAELEREARGA 420 IERYSHVMH+V+NV G + E K FDA +S LPAGT+SGAPK+RAMEII ELE RG Sbjct: 366 HIERYSHVMHVVTNVQGEIREDKTPFDALMSILPAGTLSGAPKVRAMEIIDELETVKRGP 425 Query: 421 YAGAIGYLGFSGNMDSCITIRTIIFRKGRAYVQAGAGIVWDSVPEKEYEETVNKAKAMLK 480 Y GAIGYL F+GN+DSCITIRTII + G+AYVQAGAGIV DSVPE+EYEE NKA A+LK Sbjct: 426 YGGAIGYLSFNGNLDSCITIRTIILKDGKAYVQAGAGIVADSVPEREYEECYNKAMALLK 485 Query: 481 AIRMA 485 AI A Sbjct: 486 AIEEA 490 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.138 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,840,280,404 Number of extensions: 116868323 Number of successful extensions: 305132 Number of sequences better than 1.0e-05: 3729 Number of HSP's gapped: 295694 Number of HSP's successfully gapped: 3759 Length of query: 509 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 369 Effective length of database: 1,741,754,103 Effective search space: 642707264007 Effective search space used: 642707264007 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)