BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0256.0027.p (398 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DMG3.1 B1DMG3_9BACL Tryptophan synthase, beta subunit 639 0.0 C0ZCE5.1 C0ZCE5_BREBN Tryptophan synthase beta chain EC=4.2.1.20 582 e-164 Q39SR7.1 Q39SR7_GEOMG Tryptophan synthase, beta chain EC=4.2.1.20 565 e-159 B7DPJ2.1 B7DPJ2_9BACL Tryptophan synthase, beta subunit 564 e-159 Q2B9F8.1 Q2B9F8_9BACI Tryptophan synthase subunit beta EC=4.2.1.20 563 e-158 >B1DMG3.1 B1DMG3_9BACL Tryptophan synthase, beta subunit Length = 398 Score = 639 bits (1647), Expect = 0.0, Method: Compositional matrix adjust. Identities = 302/396 (76%), Positives = 352/396 (88%) Query: 1 MIQVPDKNGRFGSFGGRFVPETLMNALIELEEAYNRFSADPAFQKEVDYLLKQYSGRETP 60 M QVPD+NGRFG FGGR+VPETLMNAL+ELEEAY +S D +F++EV LL +YSGR TP Sbjct: 1 MNQVPDENGRFGKFGGRYVPETLMNALLELEEAYKHYSQDESFKQEVHDLLHKYSGRPTP 60 Query: 61 LYYAERLSKHLGEAKIYLKREDLNHTGAHKINNAIGQGILAKMMGKTKVIAETGAGQHGV 120 LYYAERLS+HLG AKIYLKREDLNHTGAHKINN IGQ +LAK MGKTKVIAETGAGQHGV Sbjct: 61 LYYAERLSQHLGGAKIYLKREDLNHTGAHKINNTIGQAVLAKRMGKTKVIAETGAGQHGV 120 Query: 121 ATATVAALLGMECKVFMGEEDTRRQALNVFRMKLLGAEVIPVTSGSRTLKDAGNEALRYW 180 A+ATVAALLG+ECKVFMGEED +RQ LNVFRM+LLGAEV+PV SG+RTLKDA NE LRYW Sbjct: 121 ASATVAALLGLECKVFMGEEDMKRQQLNVFRMQLLGAEVVPVMSGTRTLKDACNETLRYW 180 Query: 181 VSNVEDTFYVLGSAVGPHPYPMMVRNFQRIIGDETRRQILEAEGRLPDLLVAAVGGGSNA 240 VS+V+DTFY+LGSA GPHPYPMMVR+FQRIIGDE+R+QI+EAE RLPD +VAAVGGGSNA Sbjct: 181 VSHVDDTFYILGSATGPHPYPMMVRDFQRIIGDESRKQIVEAEERLPDYVVAAVGGGSNA 240 Query: 241 IGMFYPFMEDKQVGMIGVEAAGKGVETPFHAATMSKGTRGVFQGSLSYLLQDEHGQVQEA 300 IG+FYPF++D+ V ++GVEAAG+G++T HAATM+ G+ GVFQGS+SYLLQD++GQVQ A Sbjct: 241 IGIFYPFIKDEAVRLVGVEAAGRGIDTEEHAATMTMGSHGVFQGSMSYLLQDKYGQVQPA 300 Query: 301 HSISAGLDYPGVGPEHSYLKDVHRAQYVPVTDDEALEALKLLCVTEGIIPALESAHALAH 360 HSISAGLDYPG+GPEH+YLKD RA+Y P+TD EAL+AL+LL TEGIIPALESAHA+A Sbjct: 301 HSISAGLDYPGIGPEHAYLKDSGRAEYFPITDAEALDALQLLSRTEGIIPALESAHAIAQ 360 Query: 361 VVKLGPTLTKDDLVVICLSGRGDKDVESIMAYTEGA 396 +KL PTL +D +VV+ LSGRGDKDVE+IM Y GA Sbjct: 361 TIKLAPTLDQDKIVVVSLSGRGDKDVEAIMGYLGGA 396 >C0ZCE5.1 C0ZCE5_BREBN Tryptophan synthase beta chain EC=4.2.1.20 Length = 406 Score = 582 bits (1500), Expect = e-164, Method: Compositional matrix adjust. Identities = 277/386 (71%), Positives = 325/386 (84%) Query: 4 VPDKNGRFGSFGGRFVPETLMNALIELEEAYNRFSADPAFQKEVDYLLKQYSGRETPLYY 63 VPD+NGRFG+FGG+F+PETLMNA+ ELE A+ DPAF +E++ LL +Y+GR TPL Y Sbjct: 11 VPDENGRFGAFGGKFIPETLMNAVTELEIAFEEARRDPAFVQELNGLLSEYAGRPTPLTY 70 Query: 64 AERLSKHLGEAKIYLKREDLNHTGAHKINNAIGQGILAKMMGKTKVIAETGAGQHGVATA 123 AERL+K +G A+IYLKREDLNHTGAHK+NNA+GQ +LAK MGK +IAETGAGQHGVA+A Sbjct: 71 AERLTKAVGGAQIYLKREDLNHTGAHKLNNALGQALLAKRMGKKSIIAETGAGQHGVASA 130 Query: 124 TVAALLGMECKVFMGEEDTRRQALNVFRMKLLGAEVIPVTSGSRTLKDAGNEALRYWVSN 183 TVAA LG+ CKVFMGEED RRQ+LNVFRMKLLGAEVIP SGSRTLKDA NEA+R+WVS+ Sbjct: 131 TVAAKLGLSCKVFMGEEDIRRQSLNVFRMKLLGAEVIPAVSGSRTLKDATNEAIRHWVSH 190 Query: 184 VEDTFYVLGSAVGPHPYPMMVRNFQRIIGDETRRQILEAEGRLPDLLVAAVGGGSNAIGM 243 VE+TFYV+GS VGPHPYP MVR FQ+IIG+ETR Q+L+ RLPD +VA VGGGSNAIGM Sbjct: 191 VEETFYVIGSVVGPHPYPYMVREFQKIIGEETRSQVLQMLRRLPDEVVACVGGGSNAIGM 250 Query: 244 FYPFMEDKQVGMIGVEAAGKGVETPFHAATMSKGTRGVFQGSLSYLLQDEHGQVQEAHSI 303 FYPF++D+ V + GVEAAGKGV+T HAAT++ G GV GSL+YLLQDE GQVQEAHSI Sbjct: 251 FYPFIQDESVALRGVEAAGKGVDTEKHAATLTLGRPGVIHGSLTYLLQDESGQVQEAHSI 310 Query: 304 SAGLDYPGVGPEHSYLKDVHRAQYVPVTDDEALEALKLLCVTEGIIPALESAHALAHVVK 363 SAGLDYPGVGPEH+YLKD R Y VTD EALEA+++LC TEGIIPALESAHA+A +K Sbjct: 311 SAGLDYPGVGPEHAYLKDTGRVTYTSVTDAEALEAVQVLCQTEGIIPALESAHAIAEAIK 370 Query: 364 LGPTLTKDDLVVICLSGRGDKDVESI 389 ++ D LVVICLSGRGDKDV +I Sbjct: 371 RAKEMSSDKLVVICLSGRGDKDVLTI 396 >Q39SR7.1 Q39SR7_GEOMG Tryptophan synthase, beta chain EC=4.2.1.20 Length = 396 Score = 565 bits (1456), Expect = e-159, Method: Compositional matrix adjust. Identities = 262/388 (67%), Positives = 321/388 (82%) Query: 2 IQVPDKNGRFGSFGGRFVPETLMNALIELEEAYNRFSADPAFQKEVDYLLKQYSGRETPL 61 +++PDK G FG FGGR+VPETLM AL+ELE+AYN + D F++E DY L+QY GR PL Sbjct: 1 MKLPDKQGHFGQFGGRYVPETLMPALLELEKAYNHYKNDREFKEEFDYYLRQYVGRPNPL 60 Query: 62 YYAERLSKHLGEAKIYLKREDLNHTGAHKINNAIGQGILAKMMGKTKVIAETGAGQHGVA 121 Y+AE+L++ LG AKIYLKREDLNHTGAHK+NN IGQG+LAK MGK +VIAETGAGQHGVA Sbjct: 61 YFAEKLTQKLGGAKIYLKREDLNHTGAHKVNNTIGQGLLAKRMGKKRVIAETGAGQHGVA 120 Query: 122 TATVAALLGMECKVFMGEEDTRRQALNVFRMKLLGAEVIPVTSGSRTLKDAGNEALRYWV 181 TAT+AAL GMEC+VFMGEED RRQALNVFRMKLLGA+V PVT+G+ TLKDA NEA+R WV Sbjct: 121 TATIAALFGMECEVFMGEEDIRRQALNVFRMKLLGAKVTPVTAGTATLKDAMNEAMRNWV 180 Query: 182 SNVEDTFYVLGSAVGPHPYPMMVRNFQRIIGDETRRQILEAEGRLPDLLVAAVGGGSNAI 241 + + +TFYV+G+ GPHPYP+MVR+FQ +IG E + Q + EGRLPD+LVA VGGGSNA+ Sbjct: 181 TYIHNTFYVIGTVAGPHPYPVMVRDFQSVIGREAKAQHKKIEGRLPDVLVACVGGGSNAL 240 Query: 242 GMFYPFMEDKQVGMIGVEAAGKGVETPFHAATMSKGTRGVFQGSLSYLLQDEHGQVQEAH 301 G+FYPF+ D++V M+GVEAAG G+ + HAA + GT GV G+ +YLLQDE GQ+ AH Sbjct: 241 GLFYPFLNDREVRMVGVEAAGHGIASGAHAAPLCAGTVGVLHGNKTYLLQDEFGQIANAH 300 Query: 302 SISAGLDYPGVGPEHSYLKDVHRAQYVPVTDDEALEALKLLCVTEGIIPALESAHALAHV 361 S+SAGLDYPGVGPEH++LKD RA+YV VTDDEALEA LL EGI+PALES+HA+AHV Sbjct: 301 SVSAGLDYPGVGPEHAWLKDSRRAEYVSVTDDEALEAFTLLTREEGIMPALESSHAIAHV 360 Query: 362 VKLGPTLTKDDLVVICLSGRGDKDVESI 389 VKL PTL ++ +V CLSGRGDKD+ ++ Sbjct: 361 VKLAPTLPREQTIVACLSGRGDKDIHTV 388 >B7DPJ2.1 B7DPJ2_9BACL Tryptophan synthase, beta subunit Length = 404 Score = 564 bits (1454), Expect = e-159, Method: Compositional matrix adjust. Identities = 266/387 (68%), Positives = 325/387 (83%) Query: 5 PDKNGRFGSFGGRFVPETLMNALIELEEAYNRFSADPAFQKEVDYLLKQYSGRETPLYYA 64 PD+ GR+G+FGGR+VPETLM+AL++LE+ Y R S D +FQ E+ Y L+QY+GR TPLYYA Sbjct: 7 PDETGRYGAFGGRYVPETLMSALLQLEQDYLRLSRDESFQAELKYYLEQYAGRPTPLYYA 66 Query: 65 ERLSKHLGEAKIYLKREDLNHTGAHKINNAIGQGILAKMMGKTKVIAETGAGQHGVATAT 124 ERL+KHLG KIYLKREDLNHTGAHKINNAIGQ +LA GK +VIAETGAGQHGVATAT Sbjct: 67 ERLTKHLGGPKIYLKREDLNHTGAHKINNAIGQALLALRTGKRRVIAETGAGQHGVATAT 126 Query: 125 VAALLGMECKVFMGEEDTRRQALNVFRMKLLGAEVIPVTSGSRTLKDAGNEALRYWVSNV 184 VAA G+EC VFMGEED RRQALNVFRM++LGAEV+ TSG+RTLKDA NEA+R+WV++V Sbjct: 127 VAARFGLECTVFMGEEDMRRQALNVFRMRMLGAEVVAATSGTRTLKDATNEAIRHWVAHV 186 Query: 185 EDTFYVLGSAVGPHPYPMMVRNFQRIIGDETRRQILEAEGRLPDLLVAAVGGGSNAIGMF 244 +DT Y++GS VGPHPYPM+VR+FQR+IGDE + QIL+ EGRLP +VA VGGGSNA+G+F Sbjct: 187 DDTHYIIGSVVGPHPYPMIVRDFQRVIGDEAKAQILQQEGRLPTSVVACVGGGSNAMGIF 246 Query: 245 YPFMEDKQVGMIGVEAAGKGVETPFHAATMSKGTRGVFQGSLSYLLQDEHGQVQEAHSIS 304 YPF+ED+ V +IG EAAG G+ETP+HAA++++G GV G+ + LLQDE+GQVQ AHSIS Sbjct: 247 YPFIEDEGVELIGCEAAGHGLETPYHAASIARGRPGVLHGAKTMLLQDEYGQVQPAHSIS 306 Query: 305 AGLDYPGVGPEHSYLKDVHRAQYVPVTDDEALEALKLLCVTEGIIPALESAHALAHVVKL 364 AGLDYPG+GPEH++L RA YVPVTD+EALEA LL EGIIPALESAHA+A+V++ Sbjct: 307 AGLDYPGIGPEHAHLAATGRATYVPVTDEEALEAFFLLSKLEGIIPALESAHAIAYVMRE 366 Query: 365 GPTLTKDDLVVICLSGRGDKDVESIMA 391 DD+V++CLSGRGDKDVE + A Sbjct: 367 AGRYGPDDVVIVCLSGRGDKDVEQVAA 393 >Q2B9F8.1 Q2B9F8_9BACI Tryptophan synthase subunit beta EC=4.2.1.20 Length = 402 Score = 563 bits (1450), Expect = e-158, Method: Compositional matrix adjust. Identities = 266/388 (68%), Positives = 320/388 (82%) Query: 4 VPDKNGRFGSFGGRFVPETLMNALIELEEAYNRFSADPAFQKEVDYLLKQYSGRETPLYY 63 +PD+ G FG +GGR+VPETLM A+ ELEE Y + D +F KE++ LLK Y GRETPLY+ Sbjct: 7 MPDEKGHFGIYGGRYVPETLMQAVTELEEEYKKALQDESFTKELNSLLKDYVGRETPLYF 66 Query: 64 AERLSKHLGEAKIYLKREDLNHTGAHKINNAIGQGILAKMMGKTKVIAETGAGQHGVATA 123 AE L+KH G AKI+LKREDLNHTGAHKINN IGQ +LA MGK K++AETGAGQHGVATA Sbjct: 67 AENLTKHAGGAKIFLKREDLNHTGAHKINNTIGQALLAVRMGKKKIVAETGAGQHGVATA 126 Query: 124 TVAALLGMECKVFMGEEDTRRQALNVFRMKLLGAEVIPVTSGSRTLKDAGNEALRYWVSN 183 TV ALL +EC ++MGEED RRQALNVFRM+LLGA+V+ V+SGS TLKDA NEALRYWV+N Sbjct: 127 TVCALLNLECVIYMGEEDIRRQALNVFRMELLGAKVVGVSSGSSTLKDAVNEALRYWVAN 186 Query: 184 VEDTFYVLGSAVGPHPYPMMVRNFQRIIGDETRRQILEAEGRLPDLLVAAVGGGSNAIGM 243 V DT Y+LGS +GPHP+PM+VR+FQ +IG ET++Q LE EG+LPD +VA +GGGSN++GM Sbjct: 187 VSDTHYILGSVMGPHPFPMIVRDFQSVIGAETKQQYLEKEGKLPDAVVACIGGGSNSMGM 246 Query: 244 FYPFMEDKQVGMIGVEAAGKGVETPFHAATMSKGTRGVFQGSLSYLLQDEHGQVQEAHSI 303 FYPF++DK V M GVEAAG GV+T HAA+++KG GV G+L YLLQDE GQ+QEAHSI Sbjct: 247 FYPFIKDKSVRMYGVEAAGLGVDTGNHAASLTKGKPGVLHGALMYLLQDEDGQIQEAHSI 306 Query: 304 SAGLDYPGVGPEHSYLKDVHRAQYVPVTDDEALEALKLLCVTEGIIPALESAHALAHVVK 363 SAGLDYPGVGPEHSYLKD RA Y +TD+EALEALKLLC EGIIPALESAHA+A +K Sbjct: 307 SAGLDYPGVGPEHSYLKDTGRAVYESITDEEALEALKLLCRLEGIIPALESAHAVAFALK 366 Query: 364 LGPTLTKDDLVVICLSGRGDKDVESIMA 391 L T+ + + +CLSGRGDKDVE++ A Sbjct: 367 LAGTMNNSEGIAVCLSGRGDKDVETVKA 394 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.136 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,460,200,647 Number of extensions: 108244150 Number of successful extensions: 277050 Number of sequences better than 1.0e-05: 2305 Number of HSP's gapped: 274958 Number of HSP's successfully gapped: 2307 Length of query: 398 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 260 Effective length of database: 1,760,596,133 Effective search space: 457754994580 Effective search space used: 457754994580 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)