BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0256.0026.p (273 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DMG2.1 B1DMG2_9BACL Tryptophan synthase alpha chain EC=4.2.1.20 332 1e-89 B3KKT4.1 B3KKT4_9BACI Tryptophan synthase alpha chain EC=4.2.1.20 329 1e-88 A8FEJ7.1 TRPA_BACP2 Tryptophan synthase alpha chain EC=4.2.1.20 245 3e-63 B4AKJ8.1 B4AKJ8_BACPU Tryptophan synthase alpha chain EC=4.2.1.20 242 3e-62 B7GHR0.1 B7GHR0_ANOFW Tryptophan synthase alpha chain EC=4.2.1.20 234 5e-60 >B1DMG2.1 B1DMG2_9BACL Tryptophan synthase alpha chain EC=4.2.1.20 Length = 268 Score = 332 bits (852), Expect = 1e-89, Method: Compositional matrix adjust. Identities = 160/266 (60%), Positives = 201/266 (75%) Query: 7 NRMDVAFRRLKEEGRTALIPFLTVGDPXXXXXXXXXXXXXXXXXXXXXXXVPYSDPLADG 66 N +D AF +LK+EG+TALIPFLT+GDP VPYSDPLADG Sbjct: 2 NLIDAAFAKLKQEGKTALIPFLTIGDPDIPTSIAIIKELEQAGADLVELGVPYSDPLADG 61 Query: 67 PVIQRASSRALRGNINLRTCMETALKARQAGSKLPFILFTYYNPVMQMGLDNFFSLLDAH 126 PVIQRAS RAL+ I++ C+ETA +AR+AG KLPFILFTY+NPV+Q GL++F +L+ Sbjct: 62 PVIQRASERALKNRISILDCIETAAQAREAGVKLPFILFTYFNPVLQFGLEDFMNLVVEK 121 Query: 127 EISGLIIPDLPVEESEDMRQRSRAAGVNLIPLVAPTSSERIARIVAGASGFVYCVSSLGV 186 ISGLIIPDLP+EE E++R + A GV+LIPLVAPTS +R+ RI A GFVYCVSSLGV Sbjct: 122 GISGLIIPDLPIEEDEEVRTLAEAKGVHLIPLVAPTSKDRVVRISKKARGFVYCVSSLGV 181 Query: 187 TGERSSFHSGVDDFIASVRQATDLPVAVGFGISTGEQVARFARICDGVVVGSAIVRKVED 246 TG R+ FHSG+D+F+A+VR+ATDLP+AVGFGIS+ EQV RF +ICDGVVVGSAIVRK+E+ Sbjct: 182 TGVRAEFHSGIDEFLATVREATDLPIAVGFGISSREQVERFEKICDGVVVGSAIVRKIEE 241 Query: 247 VIPLLDNPATRSEGLLQIREFVAQLR 272 +P L + + R +GL +IR FVA + Sbjct: 242 TLPKLSDSSKREDGLAEIRSFVAGFK 267 >B3KKT4.1 B3KKT4_9BACI Tryptophan synthase alpha chain EC=4.2.1.20 Length = 239 Score = 329 bits (844), Expect = 1e-88, Method: Compositional matrix adjust. Identities = 155/216 (71%), Positives = 186/216 (86%) Query: 57 VPYSDPLADGPVIQRASSRALRGNINLRTCMETALKARQAGSKLPFILFTYYNPVMQMGL 116 VPYSDPLADGPVIQRAS RAL+ + +RTCMETA AR+ GSKLPFILFTYYNPV+Q GL Sbjct: 23 VPYSDPLADGPVIQRASERALKSKVTIRTCMETARLARERGSKLPFILFTYYNPVLQTGL 82 Query: 117 DNFFSLLDAHEISGLIIPDLPVEESEDMRQRSRAAGVNLIPLVAPTSSERIARIVAGASG 176 D FF + H+ISGLIIPDLPVEESE++R+R+ AAGV+LIPLVAPTS ERIA I + A+G Sbjct: 83 DVFFEEVVRHDISGLIIPDLPVEESEEVRKRAHAAGVHLIPLVAPTSHERIASIASEATG 142 Query: 177 FVYCVSSLGVTGERSSFHSGVDDFIASVRQATDLPVAVGFGISTGEQVARFARICDGVVV 236 FVYCVSSLGVTGER+ F+SGV+ F+ +VRQ + +P+A+GFGIS+ EQVARF++ICDGVVV Sbjct: 143 FVYCVSSLGVTGERTDFYSGVESFLETVRQYSSVPIAIGFGISSSEQVARFSKICDGVVV 202 Query: 237 GSAIVRKVEDVIPLLDNPATRSEGLLQIREFVAQLR 272 GSAIVR++E IPLL+ TR +GLLQIREFVAQL+ Sbjct: 203 GSAIVRQIESTIPLLEKEETRQQGLLQIREFVAQLK 238 >A8FEJ7.1 TRPA_BACP2 Tryptophan synthase alpha chain EC=4.2.1.20 Length = 267 Score = 245 bits (626), Expect = 3e-63, Method: Compositional matrix adjust. Identities = 122/256 (47%), Positives = 160/256 (62%) Query: 18 EEGRTALIPFLTVGDPXXXXXXXXXXXXXXXXXXXXXXXVPYSDPLADGPVIQRASSRAL 77 E+G IPF+T GDP VPYSDPLADGPVIQRAS RAL Sbjct: 7 EQGEKLFIPFMTAGDPSEEITIDLALSLQAAGAHAIELGVPYSDPLADGPVIQRASKRAL 66 Query: 78 RGNINLRTCMETALKARQAGSKLPFILFTYYNPVMQMGLDNFFSLLDAHEISGLIIPDLP 137 +N+ +E A K ++ G K+P ILFTYYNPV+Q+ + FF+LL + ISGL+ PDLP Sbjct: 67 NHGMNIVKAIELAGKMKKNGVKIPVILFTYYNPVLQLDTEYFFALLRKNHISGLLTPDLP 126 Query: 138 VEESEDMRQRSRAAGVNLIPLVAPTSSERIARIVAGASGFVYCVSSLGVTGERSSFHSGV 197 EES ++++ ++ ++ I LVAPTS ER+ IV A GFVYCVSSLGVTG R +F + Sbjct: 127 FEESSELQKNCQSYDISYISLVAPTSKERLVNIVEQAEGFVYCVSSLGVTGVRQTFDESI 186 Query: 198 DDFIASVRQATDLPVAVGFGISTGEQVARFARICDGVVVGSAIVRKVEDVIPLLDNPATR 257 DFI V+Q + +PVAVGFGIST EQV ++ DGVVVGSA+V+K+E++ L TR Sbjct: 187 TDFIQQVKQLSHIPVAVGFGISTREQVDSMNKLSDGVVVGSALVKKIEELHDQLLASDTR 246 Query: 258 SEGLLQIREFVAQLRP 273 + L + + P Sbjct: 247 QDALREFETYAKTFSP 262 >B4AKJ8.1 B4AKJ8_BACPU Tryptophan synthase alpha chain EC=4.2.1.20 Length = 267 Score = 242 bits (617), Expect = 3e-62, Method: Compositional matrix adjust. Identities = 121/256 (47%), Positives = 158/256 (61%) Query: 18 EEGRTALIPFLTVGDPXXXXXXXXXXXXXXXXXXXXXXXVPYSDPLADGPVIQRASSRAL 77 E+ IPF+T GDP VPYSDPLADGPVIQRAS RAL Sbjct: 7 EQDEKLFIPFITAGDPSEEITIDLALSLQVAGAHAIELGVPYSDPLADGPVIQRASKRAL 66 Query: 78 RGNINLRTCMETALKARQAGSKLPFILFTYYNPVMQMGLDNFFSLLDAHEISGLIIPDLP 137 +N+ +E A K ++ G K+P ILFTYYNPV+Q+ + FF+LL + ISGL+ PDLP Sbjct: 67 NHGMNIVKAIELAGKMKKNGVKIPVILFTYYNPVLQLDTEYFFALLRKNHISGLLTPDLP 126 Query: 138 VEESEDMRQRSRAAGVNLIPLVAPTSSERIARIVAGASGFVYCVSSLGVTGERSSFHSGV 197 EES ++++ + ++ I LVAPTS ER+ IV A GFVYCVSSLGVTG R +F + Sbjct: 127 FEESSELQKNCQTYDISYISLVAPTSKERLVNIVEQAEGFVYCVSSLGVTGVRQTFDESI 186 Query: 198 DDFIASVRQATDLPVAVGFGISTGEQVARFARICDGVVVGSAIVRKVEDVIPLLDNPATR 257 DFI V+Q + +PVAVGFGIST EQV ++ DGVVVGSA+V+K+E++ L TR Sbjct: 187 TDFIQQVKQLSHIPVAVGFGISTREQVDAMNKLSDGVVVGSALVKKIEELQDQLLASDTR 246 Query: 258 SEGLLQIREFVAQLRP 273 + L + + P Sbjct: 247 QDALREFETYAKTFSP 262 >B7GHR0.1 B7GHR0_ANOFW Tryptophan synthase alpha chain EC=4.2.1.20 Length = 279 Score = 234 bits (598), Expect = 5e-60, Method: Compositional matrix adjust. Identities = 118/248 (47%), Positives = 154/248 (62%) Query: 24 LIPFLTVGDPXXXXXXXXXXXXXXXXXXXXXXXVPYSDPLADGPVIQRASSRALRGNINL 83 IPF+ GDP VPYSDPLADGP+IQRA+SRAL+ + L Sbjct: 4 FIPFIVAGDPCEEVTIDLAIALEQAGATILELGVPYSDPLADGPIIQRAASRALKNGMTL 63 Query: 84 RTCMETALKARQAGSKLPFILFTYYNPVMQMGLDNFFSLLDAHEISGLIIPDLPVEESED 143 +E + R+ G K+P I+FTYYNPV+Q+G + FF+L + SG++IPDLP EESE Sbjct: 64 TRALELIGRMRKKGVKIPIIVFTYYNPVLQLGEERFFALAQQNGASGVLIPDLPFEESEH 123 Query: 144 MRQRSRAAGVNLIPLVAPTSSERIARIVAGASGFVYCVSSLGVTGERSSFHSGVDDFIAS 203 MR + I LVAPTS ERIA I + A F+YCVSSLGVTG R S + +F+ Sbjct: 124 MRTLGDTYDIPYISLVAPTSHERIAMIASQAQSFLYCVSSLGVTGIRDSLPKQLHEFLQQ 183 Query: 204 VRQATDLPVAVGFGISTGEQVARFARICDGVVVGSAIVRKVEDVIPLLDNPATRSEGLLQ 263 V+ + +P+ VGFGIST EQV R ICDGVV+GSAIV+K+E + L++ TRS L+ Sbjct: 184 VKSYSRVPIVVGFGISTAEQVERLQHICDGVVIGSAIVKKIEQLQDELNDAHTRSNALIA 243 Query: 264 IREFVAQL 271 ++VA L Sbjct: 244 FSQYVASL 251 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.138 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,354,257,773 Number of extensions: 49854727 Number of successful extensions: 126381 Number of sequences better than 1.0e-05: 1559 Number of HSP's gapped: 122772 Number of HSP's successfully gapped: 1559 Length of query: 273 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 139 Effective length of database: 1,798,280,193 Effective search space: 249960946827 Effective search space used: 249960946827 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)