BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0256.0025.p (365 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KKT5.1 B3KKT5_9BACI Histidinol-phosphate aminotransferase 513 e-143 B1DMG1.1 B1DMG1_9BACL Histidinol-phosphate aminotransferase 453 e-125 C0ZCE7.1 HIS8_BREBN Histidinol-phosphate aminotransferase EC=2.6... 396 e-108 B7GHJ8.1 HIS8_ANOFW Histidinol-phosphate aminotransferase EC=2.6... 364 8e-99 Q9KCA8.1 HIS8_BACHD Histidinol-phosphate aminotransferase EC=2.6... 360 1e-97 >B3KKT5.1 B3KKT5_9BACI Histidinol-phosphate aminotransferase Length = 365 Score = 513 bits (1320), Expect = e-143, Method: Compositional matrix adjust. Identities = 244/353 (69%), Positives = 283/353 (80%) Query: 1 MNPKPNIVNLPVYKPGKPIEEVKKELGLNDVIKLASNENPYGSSPSAKAAILAELDNLYL 60 M PK IVNLPVY+PGKPIEEVKKELGL+ VIKLASNENPYG SP A AI AEL L L Sbjct: 1 MKPKAQIVNLPVYQPGKPIEEVKKELGLDVVIKLASNENPYGCSPRATEAITAELGTLSL 60 Query: 61 YPDGSAAELTSALAGHLGVQSGNIIFGCGSDEIIALIARAFFLPGDETIMADQTFSVYKS 120 YPDGSAA+LT+ LAG LG++ IIFGCGSDEIIALI RAF PGDETIMADQTFSVYK+ Sbjct: 61 YPDGSAADLTAELAGQLGIERNQIIFGCGSDEIIALIIRAFLQPGDETIMADQTFSVYKT 120 Query: 121 NADIEGAVSIEVPLADGTHDLDAMLARVTERTKVIWICNPNNPTGTIVPEEALITFLNAV 180 NADIEGA IEVPL DG HDL M V+++TK++W+CNPNNPTGTIV E L++FL+ V Sbjct: 121 NADIEGATCIEVPLVDGKHDLPGMAKAVSDKTKIVWVCNPNNPTGTIVSESELVSFLDEV 180 Query: 181 PSGVMVVLDEAYCEYVTDLSYSNGIKLLESYPNLVVLRTFSKIYGLAALRIGYGVASPQI 240 P+ VMVVLDEAY EYVTD SY + IKL+E YPNLV+LRTFSKIYGLAALRIGYG+ ++ Sbjct: 181 PAHVMVVLDEAYYEYVTDASYPDSIKLMERYPNLVILRTFSKIYGLAALRIGYGIGQQEV 240 Query: 241 ISLINKVREPFNTSRXXXXXXXXXXXDQEYVQECRRLNSAGILQLQAEFKRLDLAFFPAH 300 ISLIN+VREPFNT R DQE+V +CR++N+ GI +Q EF RL L+FFPA+ Sbjct: 241 ISLINRVREPFNTGRLAQVAAKAALKDQEFVAKCRQMNADGIHYVQGEFDRLGLSFFPAN 300 Query: 301 GNFIMVDVRRPAADIFDALLRRGIIVRAGHKLYPTYIRVTVGSAEQNKAFIAA 353 GNFIMVDV++P ++F +LL +GIIVR G +LYP YIRVTVGS EQNKAFI A Sbjct: 301 GNFIMVDVKKPGTEVFKSLLSKGIIVRPGFELYPNYIRVTVGSEEQNKAFIGA 353 >B1DMG1.1 B1DMG1_9BACL Histidinol-phosphate aminotransferase Length = 365 Score = 453 bits (1165), Expect = e-125, Method: Compositional matrix adjust. Identities = 209/353 (59%), Positives = 272/353 (77%) Query: 1 MNPKPNIVNLPVYKPGKPIEEVKKELGLNDVIKLASNENPYGSSPSAKAAILAELDNLYL 60 M PK NIV+LPVY+PGKP+E+VK+ELGL++VIKLASNENP+G S AKAAI+ ELD + Sbjct: 1 MQPKSNIVHLPVYQPGKPVEDVKRELGLDEVIKLASNENPFGYSEQAKAAIINELDQIAT 60 Query: 61 YPDGSAAELTSALAGHLGVQSGNIIFGCGSDEIIALIARAFFLPGDETIMADQTFSVYKS 120 YPDG++ ELT+A+A GV+S IIFG GS +II ++AR F PGDET+MAD+TFS YK Sbjct: 61 YPDGASFELTAAVAEKYGVESNQIIFGTGSSDIILMLARTFLAPGDETVMADETFSQYKH 120 Query: 121 NADIEGAVSIEVPLADGTHDLDAMLARVTERTKVIWICNPNNPTGTIVPEEALITFLNAV 180 NA+IE A IEVPL +G HDL AMLA++T+RTK++W+CNPNNPTGTIV EE L FL+ V Sbjct: 121 NAEIENARIIEVPLKEGKHDLPAMLAKITDRTKLVWVCNPNNPTGTIVSEEELTAFLDQV 180 Query: 181 PSGVMVVLDEAYCEYVTDLSYSNGIKLLESYPNLVVLRTFSKIYGLAALRIGYGVASPQI 240 P V+VVLDEAYCE+V + + +GIKLL+ Y N++VLRTFSKIYGLA+LRIGYG+ + Sbjct: 181 PKDVLVVLDEAYCEFVDEPGFPDGIKLLKKYRNVIVLRTFSKIYGLASLRIGYGIGHADV 240 Query: 241 ISLINKVREPFNTSRXXXXXXXXXXXDQEYVQECRRLNSAGILQLQAEFKRLDLAFFPAH 300 I +N++REPFNT+R D +++ CR+ N AGI + F RL L++FPA+ Sbjct: 241 IQYMNQIREPFNTTRLGQVAAKASLADDDFITFCRKQNRAGIEYMTNHFDRLGLSYFPAY 300 Query: 301 GNFIMVDVRRPAADIFDALLRRGIIVRAGHKLYPTYIRVTVGSAEQNKAFIAA 353 GNF+MVD + P+AD+F +LLR+G+IVR YPT+IRV+VG+ EQN+ FIAA Sbjct: 301 GNFVMVDTKLPSADVFQSLLRKGVIVRPRWTYYPTHIRVSVGTPEQNEKFIAA 353 >C0ZCE7.1 HIS8_BREBN Histidinol-phosphate aminotransferase EC=2.6.1.9 Length = 364 Score = 396 bits (1017), Expect = e-108, Method: Compositional matrix adjust. Identities = 189/353 (53%), Positives = 245/353 (69%), Gaps = 1/353 (0%) Query: 1 MNPKPNIVNLPVYKPGKPIEEVKKELGLNDVIKLASNENPYGSSPSAKAAILAELDNLYL 60 M PK I+N PVY+PGKPI++VK+E GL +VIKLASNENP+GSSP AKAAI +LDNL L Sbjct: 1 MQPKQRILNAPVYQPGKPIDDVKREFGLTEVIKLASNENPFGSSPKAKAAISEQLDNLAL 60 Query: 61 YPDGSAAELTSALAGHLGVQSGNIIFGCGSDEIIALIARAFFLPGDETIMADQTFSVYKS 120 YPDG++ L LA LGV+ + FG GSDEI+ +I+RAF G +MA QTFS Y+S Sbjct: 61 YPDGASLNLRWDLADFLGVKPSQLFFGNGSDEILLMISRAFLSEGTNAVMATQTFSQYRS 120 Query: 121 NADIEGAVSIEVPLADGTHDLDAMLARVTERTKVIWICNPNNPTGTIVPEEALITFLNAV 180 N IEGA IEVPL DG HDL+AM A + E+TKV+W+CNPNNP+GTIV L F+ Sbjct: 121 NGIIEGADLIEVPLKDGVHDLEAMAAAINEQTKVVWVCNPNNPSGTIVTTSELEAFMKKT 180 Query: 181 PSGVMVVLDEAYCEYVTDLSYSNGIKLLESYPNLVVLRTFSKIYGLAALRIGYGVASPQI 240 P V+VVLDEAY EYV D Y + +L YPNL++LRTFSKIYGLAALRIGYG+AS ++ Sbjct: 181 PKDVLVVLDEAYYEYVVDPEYPQTVPMLAEYPNLIILRTFSKIYGLAALRIGYGIASEEL 240 Query: 241 ISLINKVREPFNTSRXXXXXXXXXXXDQEYVQECRRLNSAGILQLQAEFKRLDLAFFPAH 300 IS + VREPFNT DQE+V+ CR N G+ Q F L+++P+ Sbjct: 241 ISSLEHVREPFNTGTLGQVAARAALKDQEFVKSCRDRNREGMKQFTDSFDEWGLSYYPSQ 300 Query: 301 GNFIMVDVRRPAADIFDALLRRGIIVRAGHKL-YPTYIRVTVGSAEQNKAFIA 352 NFI+VD++ + ++F LL +GIIVR+G+ L +P + R+T+G+ EQN ++ Sbjct: 301 TNFILVDLKMDSDEVFKKLLSQGIIVRSGNALGFPGFQRITIGTKEQNDKILS 353 >B7GHJ8.1 HIS8_ANOFW Histidinol-phosphate aminotransferase EC=2.6.1.9 Length = 371 Score = 364 bits (934), Expect = 8e-99, Method: Compositional matrix adjust. Identities = 179/354 (50%), Positives = 232/354 (65%), Gaps = 1/354 (0%) Query: 1 MNPKPNIVNLPVYKPGKPIEEVKKELGLNDVIKLASNENPYGSSPSAKAAILAELDNLYL 60 M K + L Y+PGKPIEEVK+E L VIKLASNENPYG SP + A++ ELD+L L Sbjct: 1 MKVKQQLKQLKPYQPGKPIEEVKREYQLETVIKLASNENPYGCSPLVRQAVMNELDDLAL 60 Query: 61 YPDGSAAELTSALAGHLGVQSGNIIFGCGSDEIIALIARAFFLPGDETIMADQTFSVYKS 120 YPDG + L ALA H+G+ +IFG GSDE++ +I RAF G T+MA TF Y+ Sbjct: 61 YPDGYSRTLREALAAHIGISEKQLIFGNGSDEVVQIICRAFLQKGTNTVMATPTFPQYRH 120 Query: 121 NADIEGAVSIEVPLADGTHDLDAMLARVTERTKVIWICNPNNPTGTIVPEEALITFLNAV 180 NA IEGA E+PL DG H L+AML + + T+V+WIC+PNNPTGT V + +L FLN V Sbjct: 121 NAIIEGAEVREIPLQDGHHHLEAMLEAIDDHTRVVWICSPNNPTGTYVNDASLRAFLNKV 180 Query: 181 PSGVMVVLDEAYCEYVTDLSYSNGIKLLESYPNLVVLRTFSKIYGLAALRIGYGVASPQI 240 P V+VV+DEAY EYV Y N + LL+ Y N+++LRTFSK YGLAALRIGYG+A + Sbjct: 181 PQHVLVVVDEAYYEYVQAEDYPNTVSLLQQYENMMILRTFSKAYGLAALRIGYGIAHEHL 240 Query: 241 ISLINKVREPFNTSRXXXXXXXXXXXDQEYVQECRRLNSAGILQLQAEFKRLDLAFFPAH 300 + I REPFNTSR DQ ++Q+C + N G+ Q A L ++ + Sbjct: 241 LQAIEPAREPFNTSRLAQAAAFAALKDQTFIQQCAQKNKQGLDQFYAFCDEYGLRYYKSE 300 Query: 301 GNFIMVDVRRPAADIFDALLRRGIIVRAGHKL-YPTYIRVTVGSAEQNKAFIAA 353 GNFI++D ++F LL+RGIIVR+G L +PT +R+TVGSAEQN+ I A Sbjct: 301 GNFILIDFGFSGDEVFTYLLQRGIIVRSGCALGFPTAVRITVGSAEQNETIIRA 354 >Q9KCA8.1 HIS8_BACHD Histidinol-phosphate aminotransferase EC=2.6.1.9 Length = 369 Score = 360 bits (923), Expect = 1e-97, Method: Compositional matrix adjust. Identities = 178/354 (50%), Positives = 234/354 (66%), Gaps = 1/354 (0%) Query: 1 MNPKPNIVNLPVYKPGKPIEEVKKELGLNDVIKLASNENPYGSSPSAKAAILAELDNLYL 60 M KP I+ LP YKPGKP+E+VK+ELGL V+KLASNENP+G+SP AI L + + Sbjct: 1 MKIKPQIIGLPPYKPGKPMEDVKRELGLETVVKLASNENPFGASPKVAEAIQEALHHTAI 60 Query: 61 YPDGSAAELTSALAGHLGVQSGNIIFGCGSDEIIALIARAFFLPGDETIMADQTFSVYKS 120 YPDG A L +A ++GV +IFG GSDE+I ++ RAF T+MA+ TFS YK Sbjct: 61 YPDGYARTLRDKVAHYVGVAPEELIFGNGSDEVIQILCRAFLNEQTNTVMAEPTFSQYKL 120 Query: 121 NADIEGAVSIEVPLADGTHDLDAMLARVTERTKVIWICNPNNPTGTIVPEEALITFLNAV 180 NA IEGA EVPL DG HDLD ML + E T+++W+CNPNNPTGT V EA +FL V Sbjct: 121 NAVIEGAELREVPLKDGVHDLDGMLEVIDENTRIVWVCNPNNPTGTYVSAEAFQSFLAKV 180 Query: 181 PSGVMVVLDEAYCEYVTDLSYSNGIKLLESYPNLVVLRTFSKIYGLAALRIGYGVASPQI 240 P V+VV DEAY EYVT Y + + ++ +Y NLVVLRTFSK++GLAALRIGYGVA+ + Sbjct: 181 PEDVLVVSDEAYVEYVTADDYPDTVPMIRTYKNLVVLRTFSKVFGLAALRIGYGVANQAL 240 Query: 241 ISLINKVREPFNTSRXXXXXXXXXXXDQEYVQECRRLNSAGILQLQAEFKRLDLAFFPAH 300 I I VR PFN S D+ +VQ N+ G+ QL R+ L +FP+ Sbjct: 241 IEKIEPVRPPFNNSTFSQVAAVAALEDEAFVQSSVEKNNEGMEQLTEWCSRMGLDYFPSQ 300 Query: 301 GNFIMVDVRRPAADIFDALLRRGIIVRAGHKL-YPTYIRVTVGSAEQNKAFIAA 353 NF+++ V R + D+F+A+L++G I+R+G L YP +IR+T+GS EQN +AA Sbjct: 301 TNFLLIHVNRSSDDVFNAMLKKGYIIRSGRALGYPAWIRITIGSKEQNDGCLAA 354 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.137 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,008,541,845 Number of extensions: 80272488 Number of successful extensions: 220737 Number of sequences better than 1.0e-05: 5334 Number of HSP's gapped: 214985 Number of HSP's successfully gapped: 5335 Length of query: 365 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 228 Effective length of database: 1,770,017,148 Effective search space: 403563909744 Effective search space used: 403563909744 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)