BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0256.0012.p (223 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KKU5.1 B3KKU5_9BACI Cytochrome b/b6 domain 399 e-110 B1DM16.1 B1DM16_9BACL Cytochrome b/b6 domain 396 e-109 Q9KCA0.1 Q9KCA0_BACHD Menaquinol-cytochrome c reductase (Cytochr... 374 e-102 B1HTE4.1 B1HTE4_LYSSC Menaquinol-cytochrome c reductase cytochro... 370 e-101 A3IG40.1 A3IG40_9BACI Cytochrome b6 370 e-101 >B3KKU5.1 B3KKU5_9BACI Cytochrome b/b6 domain Length = 223 Score = 399 bits (1026), Expect = e-110, Method: Compositional matrix adjust. Identities = 213/223 (95%), Positives = 219/223 (98%) Query: 1 MFKNVYNWIDERLDITPIWRDVADHEVPEHVNPAHHFSAFVYCFGGLTFFITVIQILSGM 60 M KNVYNWIDERLDITPIWRDVADHEVPEHVNPAHHFSAFVYCFGGLTFFITVIQILSGM Sbjct: 1 MLKNVYNWIDERLDITPIWRDVADHEVPEHVNPAHHFSAFVYCFGGLTFFITVIQILSGM 60 Query: 61 FLTMYYVPDIINAYASVEYLQTKVAFGQIVRGMHHWGASLVIVMMFLHTMRVFFTGSYKA 120 FLTMYYVPDIINAY SVEYLQT+VAFGQIVRGMHHWGASLVIVMMFLHTMRVFFTGSYKA Sbjct: 61 FLTMYYVPDIINAYNSVEYLQTQVAFGQIVRGMHHWGASLVIVMMFLHTMRVFFTGSYKA 120 Query: 121 PREMNWVVGMLIFFVMLGLGLTGYLLPWDNKAYFATKVTLEIANSVPVMGPVLKELMQGG 180 PREMNWVVGMLIFFVMLGLGLTGYLLPWDNKAYFATKVTLEIANSVP +GP++KEL+QGG Sbjct: 121 PREMNWVVGMLIFFVMLGLGLTGYLLPWDNKAYFATKVTLEIANSVPWLGPIIKELLQGG 180 Query: 181 TIVGAETLTRFFALHVFFLPAVLLILLVGHFIMIRRQGISGPL 223 TIVGAETLTRFFA+HVFFLPAVLL LLVGHFIMIRRQGISGPL Sbjct: 181 TIVGAETLTRFFAIHVFFLPAVLLALLVGHFIMIRRQGISGPL 223 >B1DM16.1 B1DM16_9BACL Cytochrome b/b6 domain Length = 223 Score = 396 bits (1018), Expect = e-109, Method: Compositional matrix adjust. Identities = 188/223 (84%), Positives = 206/223 (92%) Query: 1 MFKNVYNWIDERLDITPIWRDVADHEVPEHVNPAHHFSAFVYCFGGLTFFITVIQILSGM 60 MFK++YNWIDERLDITP+WRDVADHEVPEHVNPAHHFSAFVYCFGGLTFFITVIQILSGM Sbjct: 1 MFKSMYNWIDERLDITPLWRDVADHEVPEHVNPAHHFSAFVYCFGGLTFFITVIQILSGM 60 Query: 61 FLTMYYVPDIINAYASVEYLQTKVAFGQIVRGMHHWGASLVIVMMFLHTMRVFFTGSYKA 120 FLTMYYVPDIINAYASV+YL KVAFG IVRGMHH+GASLVIVMMFLHT+RVFFTGSYKA Sbjct: 61 FLTMYYVPDIINAYASVDYLNHKVAFGGIVRGMHHFGASLVIVMMFLHTLRVFFTGSYKA 120 Query: 121 PREMNWVVGMLIFFVMLGLGLTGYLLPWDNKAYFATKVTLEIANSVPVMGPVLKELMQGG 180 PREMNWVVGMLIFF+MLGLG TGYLLPWDNKAYFATKV ++IA SVP +GP + E + GG Sbjct: 121 PREMNWVVGMLIFFIMLGLGFTGYLLPWDNKAYFATKVGVQIAESVPYIGPYIGEFLYGG 180 Query: 181 TIVGAETLTRFFALHVFFLPAVLLILLVGHFIMIRRQGISGPL 223 IVGA+TLTRFFA+HVFFLP L+ +L HF+MIR+QGISGPL Sbjct: 181 DIVGAQTLTRFFAIHVFFLPGALIAMLAAHFLMIRKQGISGPL 223 >Q9KCA0.1 Q9KCA0_BACHD Menaquinol-cytochrome c reductase (Cytochrome b subunit) Length = 223 Score = 374 bits (959), Expect = e-102, Method: Compositional matrix adjust. Identities = 177/223 (79%), Positives = 197/223 (88%) Query: 1 MFKNVYNWIDERLDITPIWRDVADHEVPEHVNPAHHFSAFVYCFGGLTFFITVIQILSGM 60 M + +Y+WIDER D+TPIWRD+ADHEVPEHVNPAHHFSAFVYCFGGLTFF+TVIQILSGM Sbjct: 1 MLQRLYDWIDERFDLTPIWRDIADHEVPEHVNPAHHFSAFVYCFGGLTFFVTVIQILSGM 60 Query: 61 FLTMYYVPDIINAYASVEYLQTKVAFGQIVRGMHHWGASLVIVMMFLHTMRVFFTGSYKA 120 FLTMYYVPDIINAY SV YLQ +VAFG IVRGMHHWGASLVIVMMFLHT+RVFFTGSYK Sbjct: 61 FLTMYYVPDIINAYQSVAYLQNEVAFGVIVRGMHHWGASLVIVMMFLHTLRVFFTGSYKK 120 Query: 121 PREMNWVVGMLIFFVMLGLGLTGYLLPWDNKAYFATKVTLEIANSVPVMGPVLKELMQGG 180 PRE+NWV+G+LIFFVMLGLG TGYLLPWD KAYFAT V L+IA SVP++G K L+ GG Sbjct: 121 PRELNWVIGVLIFFVMLGLGFTGYLLPWDMKAYFATVVGLQIAESVPIIGDFAKTLLAGG 180 Query: 181 TIVGAETLTRFFALHVFFLPAVLLILLVGHFIMIRRQGISGPL 223 I+GA TL RFFA+HVFFLPA LL L+ HF +IR+QGISGPL Sbjct: 181 EIIGASTLARFFAIHVFFLPAALLGLIGVHFYLIRKQGISGPL 223 >B1HTE4.1 B1HTE4_LYSSC Menaquinol-cytochrome c reductase cytochrome b subunit Length = 224 Score = 370 bits (951), Expect = e-101, Method: Compositional matrix adjust. Identities = 178/224 (79%), Positives = 200/224 (89%), Gaps = 1/224 (0%) Query: 1 MFKNVYNWIDERLDITPIWRDVADHEVPEHVNPAHHFSAFVYCFGGLTFFITVIQILSGM 60 M +Y+W+DERLDITPIWRD+ADHEVPEHVNPAHHFSAFVYCFGGLTFFITVIQILSGM Sbjct: 1 MLNKIYDWVDERLDITPIWRDIADHEVPEHVNPAHHFSAFVYCFGGLTFFITVIQILSGM 60 Query: 61 FLTMYYVPDIINAYASVEYLQTKVAFGQIVRGMHHWGASLVIVMMFLHTMRVFFTGSYKA 120 FLTMYYVPD++NA+ SV YLQ +VAFG+IVRGMHHWGASLVIVMMFLHT+RVFFTGSYK Sbjct: 61 FLTMYYVPDVVNAWKSVYYLQNEVAFGEIVRGMHHWGASLVIVMMFLHTLRVFFTGSYKK 120 Query: 121 PREMNWVVGMLIFFVMLGLGLTGYLLPWDNKAYFATKVTLEIANSVPVMGPVLKELMQGG 180 PRE+NW+VG+ IF VM+GLG TGYLLPWD KA FATKV +EIA SVP +G ++K L+ G Sbjct: 121 PRELNWMVGVGIFGVMMGLGFTGYLLPWDMKALFATKVGIEIAASVPFIGSMIKVLLAGD 180 Query: 181 -TIVGAETLTRFFALHVFFLPAVLLILLVGHFIMIRRQGISGPL 223 TI+GA+TLTRFFA+HVFFLPAVL LL HFIMIRRQGISGPL Sbjct: 181 TTILGAQTLTRFFAIHVFFLPAVLFGLLAAHFIMIRRQGISGPL 224 >A3IG40.1 A3IG40_9BACI Cytochrome b6 Length = 224 Score = 370 bits (951), Expect = e-101, Method: Compositional matrix adjust. Identities = 178/224 (79%), Positives = 200/224 (89%), Gaps = 1/224 (0%) Query: 1 MFKNVYNWIDERLDITPIWRDVADHEVPEHVNPAHHFSAFVYCFGGLTFFITVIQILSGM 60 M +Y+W+DERLDITPIWRD+ADHEVPEHVNPAHHFSAFVYCFGGLTFFITVIQILSGM Sbjct: 1 MLNKIYDWVDERLDITPIWRDIADHEVPEHVNPAHHFSAFVYCFGGLTFFITVIQILSGM 60 Query: 61 FLTMYYVPDIINAYASVEYLQTKVAFGQIVRGMHHWGASLVIVMMFLHTMRVFFTGSYKA 120 FLTMYYVPD++NA+ SV YLQ +VAFG+IVRGMHHWGASLVIVMMFLHT+RVFFTGSYK Sbjct: 61 FLTMYYVPDVVNAWKSVYYLQNEVAFGEIVRGMHHWGASLVIVMMFLHTLRVFFTGSYKK 120 Query: 121 PREMNWVVGMLIFFVMLGLGLTGYLLPWDNKAYFATKVTLEIANSVPVMGPVLKELMQGG 180 PRE+NW+VG+ IF VM+GLG TGYLLPWD KA FATKV +EIA SVP +G ++K L+ G Sbjct: 121 PRELNWMVGVGIFGVMMGLGFTGYLLPWDMKALFATKVGIEIAASVPFIGSMIKVLLAGD 180 Query: 181 -TIVGAETLTRFFALHVFFLPAVLLILLVGHFIMIRRQGISGPL 223 TI+GA+TLTRFFA+HVFFLPAVL LL HFIMIRRQGISGPL Sbjct: 181 TTILGAQTLTRFFAIHVFFLPAVLFGLLAAHFIMIRRQGISGPL 224 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.332 0.145 0.466 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,351,295,399 Number of extensions: 54031632 Number of successful extensions: 315225 Number of sequences better than 1.0e-05: 59321 Number of HSP's gapped: 249375 Number of HSP's successfully gapped: 59330 Length of query: 223 Length of database: 3,060,696,203 Length adjustment: 131 Effective length of query: 92 Effective length of database: 1,826,543,238 Effective search space: 168041977896 Effective search space used: 168041977896 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 128 (53.9 bits)