BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0256.0003.p (140 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DM08.1 B1DM08_9BACL Methylglyoxal synthase EC=4.2.3.3 249 5e-65 B3KKV3.1 B3KKV3_9BACI Methylglyoxal synthase EC=4.2.3.3 248 1e-64 B7GHS5.1 B7GHS5_ANOFW Methylglyoxal synthase 206 4e-52 C1P6W0.1 C1P6W0_BACCO Methylglyoxal synthase EC=4.2.3.3 206 7e-52 Q5KXW7.1 MGSA_GEOKA Methylglyoxal synthase EC=4.2.3.3 199 6e-50 >B1DM08.1 B1DM08_9BACL Methylglyoxal synthase EC=4.2.3.3 Length = 141 Score = 249 bits (637), Expect = 5e-65, Method: Compositional matrix adjust. Identities = 116/139 (83%), Positives = 127/139 (91%) Query: 1 MLKIAFIAHDRKKDEMVNFVTAYEHVFVGHQLYSTGTTGQRIMEVTKLSIHRYMSGPLGG 60 MLKI+FIAHDRKK+EMVNFVTAYE VF H+LYSTGTTG RIME T L I R+MSGPLGG Sbjct: 1 MLKISFIAHDRKKEEMVNFVTAYESVFAPHELYSTGTTGTRIMEKTNLQIQRFMSGPLGG 60 Query: 61 DQQIGSMVAMDELDLIIFLRDPLMAQPHEPDITALLRLCDVYGIPVATNIATAEILVKAI 120 DQQIG+MVA +E+DLIIFLRDPLMAQPHEPDI ALLRLCDV GIPVATN+ATAE+LV+A+ Sbjct: 61 DQQIGAMVAQNEMDLIIFLRDPLMAQPHEPDIIALLRLCDVQGIPVATNVATAELLVRAL 120 Query: 121 DRGDFAWRELVHKYKPGVD 139 RGDFAWRELVHKYKPGVD Sbjct: 121 ARGDFAWRELVHKYKPGVD 139 >B3KKV3.1 B3KKV3_9BACI Methylglyoxal synthase EC=4.2.3.3 Length = 139 Score = 248 bits (633), Expect = 1e-64, Method: Compositional matrix adjust. Identities = 116/138 (84%), Positives = 128/138 (92%) Query: 2 LKIAFIAHDRKKDEMVNFVTAYEHVFVGHQLYSTGTTGQRIMEVTKLSIHRYMSGPLGGD 61 + IAFIAHDRKK+EMVNFV AYE+VF H+LYSTGTTG RIME TKLSIHR+ SGPLGGD Sbjct: 1 MNIAFIAHDRKKEEMVNFVIAYENVFEDHKLYSTGTTGTRIMEQTKLSIHRFASGPLGGD 60 Query: 62 QQIGSMVAMDELDLIIFLRDPLMAQPHEPDITALLRLCDVYGIPVATNIATAEILVKAID 121 QQIG++VA +E+DLIIFLRDPLMAQPHEPDI ALLRLCDV GIP+ATN+ATAEILVKA+D Sbjct: 61 QQIGALVADNEMDLIIFLRDPLMAQPHEPDINALLRLCDVQGIPLATNVATAEILVKALD 120 Query: 122 RGDFAWRELVHKYKPGVD 139 RGDFAWRELVHKYKPGVD Sbjct: 121 RGDFAWRELVHKYKPGVD 138 >B7GHS5.1 B7GHS5_ANOFW Methylglyoxal synthase Length = 142 Score = 206 bits (525), Expect = 4e-52, Method: Compositional matrix adjust. Identities = 87/134 (64%), Positives = 115/134 (85%) Query: 2 LKIAFIAHDRKKDEMVNFVTAYEHVFVGHQLYSTGTTGQRIMEVTKLSIHRYMSGPLGGD 61 LKIA IAHD+KK++MV FVTAY+H+ H+LY+TGTTG RI E T LS+HR+ SGPLGGD Sbjct: 8 LKIALIAHDKKKEDMVQFVTAYQHILAEHELYATGTTGLRIQEATGLSVHRFQSGPLGGD 67 Query: 62 QQIGSMVAMDELDLIIFLRDPLMAQPHEPDITALLRLCDVYGIPVATNIATAEILVKAID 121 QQIG+M+A +++D++IF RDPL AQPHEPD++AL+RLCDVY IP+ATN+ TAE+L++ ++ Sbjct: 68 QQIGAMIANNDMDMVIFFRDPLTAQPHEPDVSALIRLCDVYAIPLATNMGTAEVLIRGLE 127 Query: 122 RGDFAWRELVHKYK 135 RGDFAWR +V + K Sbjct: 128 RGDFAWRNIVKRGK 141 >C1P6W0.1 C1P6W0_BACCO Methylglyoxal synthase EC=4.2.3.3 Length = 140 Score = 206 bits (523), Expect = 7e-52, Method: Compositional matrix adjust. Identities = 92/132 (69%), Positives = 111/132 (84%) Query: 2 LKIAFIAHDRKKDEMVNFVTAYEHVFVGHQLYSTGTTGQRIMEVTKLSIHRYMSGPLGGD 61 +KIAFIAHD+KK E+VNF AYE VF H+LYSTGTTG+RIME T L+I R+ SGPLGGD Sbjct: 1 MKIAFIAHDKKKKEIVNFAVAYEPVFADHELYSTGTTGKRIMEKTNLNITRFNSGPLGGD 60 Query: 62 QQIGSMVAMDELDLIIFLRDPLMAQPHEPDITALLRLCDVYGIPVATNIATAEILVKAID 121 Q+IG+M+A +E+DL+ F RDPL AQPHEPDITALLRLCDVY IP+ATN+ TAE+LV+ + Sbjct: 61 QEIGAMIAKNEMDLVFFFRDPLTAQPHEPDITALLRLCDVYSIPLATNMGTAELLVQGLM 120 Query: 122 RGDFAWRELVHK 133 RGD WR LVH+ Sbjct: 121 RGDLNWRNLVHE 132 >Q5KXW7.1 MGSA_GEOKA Methylglyoxal synthase EC=4.2.3.3 Length = 144 Score = 199 bits (507), Expect = 6e-50, Method: Compositional matrix adjust. Identities = 87/139 (62%), Positives = 111/139 (79%) Query: 2 LKIAFIAHDRKKDEMVNFVTAYEHVFVGHQLYSTGTTGQRIMEVTKLSIHRYMSGPLGGD 61 +KIA IAHD+KK+EMV F TAY V H+LY+TGTTG RI E T L +HR+ SGP GGD Sbjct: 1 MKIALIAHDQKKEEMVAFATAYAPVLANHELYATGTTGLRIQEATGLPVHRFQSGPYGGD 60 Query: 62 QQIGSMVAMDELDLIIFLRDPLMAQPHEPDITALLRLCDVYGIPVATNIATAEILVKAID 121 Q+IG+M+A +E+DL+IF RDPL AQPHEPDI+AL+RLCDVY +P+ATNI TAE+L++A++ Sbjct: 61 QEIGAMIARNEMDLVIFFRDPLTAQPHEPDISALMRLCDVYAVPLATNIGTAELLIRALE 120 Query: 122 RGDFAWRELVHKYKPGVDE 140 RGD AWR +V G +E Sbjct: 121 RGDLAWRSIVRGQTKGGEE 139 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.324 0.140 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 804,740,362 Number of extensions: 28772784 Number of successful extensions: 63520 Number of sequences better than 1.0e-05: 687 Number of HSP's gapped: 63405 Number of HSP's successfully gapped: 687 Length of query: 140 Length of database: 3,060,696,203 Length adjustment: 104 Effective length of query: 36 Effective length of database: 2,080,910,643 Effective search space: 74912783148 Effective search space used: 74912783148 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 125 (52.8 bits)