BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0255.0009.p (412 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C3IAM6.1 C3IAM6_BACTU Multidrug resistance protein 138 1e-30 C3FVU6.1 C3FVU6_BACTB Multidrug resistance protein 135 5e-30 C3DCF4.1 C3DCF4_BACTU Multidrug resistance protein 135 5e-30 B7JJL9.1 B7JJL9_BACC0 Multidrug resistance protein; putative tet... 56 7e-06 Q6HK46.1 Q6HK46_BACHK Multidrug resistance protein; possible tet... 56 7e-06 >C3IAM6.1 C3IAM6_BACTU Multidrug resistance protein Length = 417 Score = 138 bits (347), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 69/167 (41%), Positives = 100/167 (59%) Query: 9 QAAPAVHSYPKLWANKKFMLLLCSFSISLFGNTFHSIALSLWVLQTTGSAKLVAVXXXXX 68 +A AV S LW N+ F + +++S+FGN+FHSIAL+LWVLQ TGSAKL++ Sbjct: 8 DSALAVSSKKTLWKNRTFTFVFTGYTLSMFGNSFHSIALNLWVLQATGSAKLMSTIAITH 67 Query: 69 XXXXXXXXXXXXXFADRTNRRKIMLITDXXXXXXXXXXXXXXXXXXXXFIIVAVLTALTT 128 ADR +RRK+M ITD FI++ +LTALT+ Sbjct: 68 MVMSMLFSSIAGTVADRVDRRKLMWITDIIRSLLILSIAFCIAIPGTPFILIIILTALTS 127 Query: 129 VSALFQSPAYQSSIVTVVGKEHVQQAVGMLNLSENICRTVGFSVGGI 175 + LFQSPA+ +S+V +VGK+ VQQA G+LN+++N+ R G ++GG+ Sbjct: 128 CAGLFQSPAFHASLVDIVGKDKVQQATGVLNVADNVSRISGLAIGGV 174 >C3FVU6.1 C3FVU6_BACTB Multidrug resistance protein Length = 417 Score = 135 bits (341), Expect = 5e-30, Method: Compositional matrix adjust. Identities = 67/167 (40%), Positives = 99/167 (59%) Query: 9 QAAPAVHSYPKLWANKKFMLLLCSFSISLFGNTFHSIALSLWVLQTTGSAKLVAVXXXXX 68 +A A S LW N+ F + +++S+FGN+FHSIAL+LWVLQ TGSAKL++ Sbjct: 8 DSALAASSKKTLWKNRTFTFVFTGYTLSMFGNSFHSIALNLWVLQATGSAKLMSTIAITH 67 Query: 69 XXXXXXXXXXXXXFADRTNRRKIMLITDXXXXXXXXXXXXXXXXXXXXFIIVAVLTALTT 128 ADR +RRK+M ITD FI++ +LTALT+ Sbjct: 68 MVMSMLFSSIAGTVADRVDRRKLMWITDIIRSLLILSIAFCIAIPGTPFILIIILTALTS 127 Query: 129 VSALFQSPAYQSSIVTVVGKEHVQQAVGMLNLSENICRTVGFSVGGI 175 + LFQSPA+ +S++ +VGK+ VQQA G+LN+++N+ R G ++GG+ Sbjct: 128 CAGLFQSPAFHASLIDIVGKDKVQQATGVLNVADNVSRISGLAIGGV 174 >C3DCF4.1 C3DCF4_BACTU Multidrug resistance protein Length = 417 Score = 135 bits (341), Expect = 5e-30, Method: Compositional matrix adjust. Identities = 67/167 (40%), Positives = 99/167 (59%) Query: 9 QAAPAVHSYPKLWANKKFMLLLCSFSISLFGNTFHSIALSLWVLQTTGSAKLVAVXXXXX 68 +A A S LW N+ F + +++S+FGN+FHSIAL+LWVLQ TGSAKL++ Sbjct: 8 DSALAASSKKTLWKNRTFTFVFTGYTLSMFGNSFHSIALNLWVLQATGSAKLMSTIAITH 67 Query: 69 XXXXXXXXXXXXXFADRTNRRKIMLITDXXXXXXXXXXXXXXXXXXXXFIIVAVLTALTT 128 ADR +RRK+M ITD FI++ +LTALT+ Sbjct: 68 MVMSMLFSSIAGTVADRVDRRKLMWITDIIRSLLILSIAFCIAIPGTPFILIIILTALTS 127 Query: 129 VSALFQSPAYQSSIVTVVGKEHVQQAVGMLNLSENICRTVGFSVGGI 175 + LFQSPA+ +S++ +VGK+ VQQA G+LN+++N+ R G ++GG+ Sbjct: 128 CAGLFQSPAFHASLIDIVGKDKVQQATGVLNVADNVSRISGLAIGGV 174 >B7JJL9.1 B7JJL9_BACC0 Multidrug resistance protein; putative tetracycline resistance determinant Length = 406 Score = 55.8 bits (133), Expect = 7e-06, Method: Compositional matrix adjust. Identities = 45/152 (29%), Positives = 73/152 (48%), Gaps = 2/152 (1%) Query: 15 HSYPKLWANKKFMLLLCSFSISLFGNTFHSIALSLWVLQTTGSAKLVAVXXXXXXXXXXX 74 +S +W+NK F + ISL GN+ ++ L LWV++ T S LV+ Sbjct: 11 NSTSSIWSNKIFSYFFLASCISLIGNSMVTLVLPLWVMKLTNSPLLVSGVNVAIATAAIL 70 Query: 75 XXXXXXXFADRTNRRKIMLITDXXXXXXXXXXXXXXXXXXXXFIIVAVLTALTTV-SALF 133 ADR +RRK+M+I D +I + +L + ++ SALF Sbjct: 71 FAPVTGTLADRMSRRKLMIIADVMRCIVMILIAVIAFYNKMLYIPLLILLIIRSIGSALF 130 Query: 134 QSPAYQSSIVTVVGKEHVQQAVGMLNLSENIC 165 +PA +++VT V ++HVQQA+ + +S I Sbjct: 131 -TPASNAALVTYVEEKHVQQAITLRQISIQII 161 >Q6HK46.1 Q6HK46_BACHK Multidrug resistance protein; possible tetracycline resistance determinant Length = 406 Score = 55.8 bits (133), Expect = 7e-06, Method: Compositional matrix adjust. Identities = 45/152 (29%), Positives = 73/152 (48%), Gaps = 2/152 (1%) Query: 15 HSYPKLWANKKFMLLLCSFSISLFGNTFHSIALSLWVLQTTGSAKLVAVXXXXXXXXXXX 74 +S +W+NK F + ISL GN+ ++ L LWV++ T S LV+ Sbjct: 11 NSTSSIWSNKIFSYFFLASCISLIGNSMVTLVLPLWVMKLTNSPLLVSGVNVAIATAAIL 70 Query: 75 XXXXXXXFADRTNRRKIMLITDXXXXXXXXXXXXXXXXXXXXFIIVAVLTALTTV-SALF 133 ADR +RRK+M+I D +I + +L + ++ SALF Sbjct: 71 FAPVTGTLADRMSRRKLMIIADVMRCIVMILIAVIAFYNKMLYIPLLILLIIRSIGSALF 130 Query: 134 QSPAYQSSIVTVVGKEHVQQAVGMLNLSENIC 165 +PA +++VT V ++HVQQA+ + +S I Sbjct: 131 -TPASNAALVTYVEEKHVQQAITLRQISIQII 161 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.324 0.137 0.434 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,228,291,104 Number of extensions: 88250759 Number of successful extensions: 186742 Number of sequences better than 1.0e-05: 50 Number of HSP's gapped: 187230 Number of HSP's successfully gapped: 50 Length of query: 412 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 274 Effective length of database: 1,760,596,133 Effective search space: 482403340442 Effective search space used: 482403340442 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 132 (55.5 bits)