BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0255.0008.p (298 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B2TMA2.1 B2TMA2_CLOBB Transcriptional regulator, LacI family 142 3e-32 A7B786.1 A7B786_RUMGN Putative uncharacterized protein 133 2e-29 Q48Y93.1 Q48Y93_STRP1 Transcriptional regulator, LacI family 79 7e-13 B4U478.1 B4U478_STREM Transcriptional regulator LacI family 79 7e-13 C0MBU1.1 C0MBU1_STRE4 LacI family regulatory protein 78 8e-13 >B2TMA2.1 B2TMA2_CLOBB Transcriptional regulator, LacI family Length = 305 Score = 142 bits (358), Expect = 3e-32, Method: Compositional matrix adjust. Identities = 92/297 (30%), Positives = 164/297 (55%), Gaps = 7/297 (2%) Query: 1 MKDIAIRANVSVATVSYVLNRVDNQTIPEKTRRQILQLAEELHYIPNLAARSLANQKTGL 60 +KDIA ANVS ATVSYVLN + + I +TR ++ + A +L+Y+PN+ ARSLA+QK+ L Sbjct: 8 LKDIAKEANVSPATVSYVLNYSEKEKISHETRLKVFEAARKLNYVPNMTARSLASQKSYL 67 Query: 61 VGVLVHRSRQLPYWKLHAQLAFIHTLEQRLTAAGYHTLLYSLDTGNPSLDIIVERKLEAV 120 +GV+++ S + K++ Q + ++++L + GY + + I +R L+AV Sbjct: 68 IGVIINLSEKNRKSKIYGQYNLVKEIQKKLYSIGYDLIFLPTKELQKDIQISQKRCLDAV 127 Query: 121 FLIDAQDASFYSISRHFVEGIPLILIDSMIEDKLFKQVVYNYRSALEKALPDDL-SQVCL 179 F+ID I HF +P+I IDS ++D +F +V+ +Y A+ L + L + + Sbjct: 128 FIIDMPSDQLKEIVNHFY--VPVIFIDSYLDDPIFNKVLTDY-EAIFNMLSEQLEKEFYV 184 Query: 180 IMESFNNSLLTRYIRSCVPLSDDAVFEIRELQDLLSATAHHRFKQAIVINEFIGSYVEKS 239 ++E ++N L + +R V + +F + DL+ ++ K+ I+ E +G EK Sbjct: 185 VIEDYSNDELLKIVRKKV--LEKNIFINKFDNDLIEFLHRNQNKKGIIFGEILGMQAEKI 242 Query: 240 GDFEHLTVICTCNSPQMLGERVEKILFQG-DKAEVAFELMQELLYHPDYSEQENNCY 295 D +L V+ + ML ++I+ +KA+ A E+M++L+Y + + E Y Sbjct: 243 VDNRNLVVVINSENDTMLLPDTKRIIVSDKEKAKKAVEIMEKLIYLENDKDIEKTSY 299 >A7B786.1 A7B786_RUMGN Putative uncharacterized protein Length = 316 Score = 133 bits (335), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 88/283 (31%), Positives = 151/283 (53%), Gaps = 5/283 (1%) Query: 1 MKDIAIRANVSVATVSYVLNRVDNQTIPEKTRRQILQLAEELHYIPNLAARSLANQKTGL 60 MKD+A A VS ATVSYVLN D + I +TR ++ + A +L+Y+PN+AA++L+ +K+ L Sbjct: 17 MKDVAKEAGVSTATVSYVLNYSDTERISHETRLRVFEAANKLNYVPNMAAKALSEKKSYL 76 Query: 61 VGVLVHRSRQLPYWKLHAQLAFIHTLEQRLTAAGYHTLLYSLDTGNPSLDIIVERKLEAV 120 VG+++++ + K++ + L A GY L S + + I + R L+AV Sbjct: 77 VGMIINKRARSKKSKIYQYYDLADEIRVILHALGYDMFLISAEEIEEGVSIGIRRTLDAV 136 Query: 121 FLIDAQDASFYSISRHFVEGIPLILIDSMIEDKLFKQVVYNYRSALEKALPDDLSQVCLI 180 F++D +++ F I+ F +P I ID + D LF +V+ + + L+KA + ++ Sbjct: 137 FIVDLEESLFKKIANKFF--VPAIFIDGYVADPLFCKVLVDSKELLDKAQEILGEEFYVV 194 Query: 181 MESFNNSLLTRYIRSCVPLSDDAVFEIRELQDLLSATAHHRFKQAIVINEFIGSYVEKSG 240 +E + N L YIR V + +F + +L H+ K+ ++I E +G VE Sbjct: 195 LEDYFNDYLLEYIRKRV--KQENIFVNKSGSNLPEFLQSHQQKKGLIIGEILGVQVENYV 252 Query: 241 DFEHLTVICTCNSPQMLGERVEKILFQG-DKAEVAFELMQELL 282 D ++ V+ ML + I+ +KA A E+MQ+LL Sbjct: 253 DNRNICVVVHSEKDIMLLPDTKIIVVSNKEKARKAVEVMQKLL 295 >Q48Y93.1 Q48Y93_STRP1 Transcriptional regulator, LacI family Length = 266 Score = 78.6 bits (192), Expect = 7e-13, Method: Compositional matrix adjust. Identities = 61/172 (35%), Positives = 91/172 (52%), Gaps = 16/172 (9%) Query: 1 MKDIAIRANVSVATVSYVLNRVDNQTIPEKTRRQILQLAEELHYIPNLAARSLANQKTGL 60 +KDIA A VSVATVSYVLN+ ++Q I E+TR ++L ++ L+Y PN AARSL Q++ Sbjct: 8 IKDIAREAGVSVATVSYVLNQRNDQKISEETRNKVLHISNLLNYTPNQAARSLVTQQSNS 67 Query: 61 VGVLVHRSRQLPYWKLHAQLAFIHTLEQRLTAAGYHTLLYSLDTGNPSLDIIVERKLEAV 120 V + Y+K + + EQ T + LL+ D L+ K + Sbjct: 68 VVL---------YYKYADSI--LARAEQLHTISFLSRLLHEQDYQLVCLNEDSTEKFDWA 116 Query: 121 FLI---DAQDASFYSISRHFVEGIPLILIDSMIEDKLFKQVVYNYRSALEKA 169 LI D + +FY I + + PLI IDS + D LF Q+ +Y + ++A Sbjct: 117 DLILTLDVDNDTFYKIGK--LNFKPLIAIDSFVNDPLFFQINTDYAALKDQA 166 >B4U478.1 B4U478_STREM Transcriptional regulator LacI family Length = 265 Score = 78.6 bits (192), Expect = 7e-13, Method: Compositional matrix adjust. Identities = 64/209 (30%), Positives = 99/209 (47%), Gaps = 30/209 (14%) Query: 1 MKDIAIRANVSVATVSYVLNRVDNQTIPEKTRRQILQLAEELHYIPNLAARSLANQKTGL 60 +KD+A A VSVATVSYV+N ++Q I +TR ++LQ+ L+Y PN AA+SL QK L Sbjct: 7 IKDVAREAGVSVATVSYVINNREDQKISPETRNKVLQMINLLNYRPNQAAKSLVTQKRQL 66 Query: 61 VGVLVHRSRQLPYWKLHA-------QLAFIHTLEQRLTAAGYHTLLYSLDTGNPSLDIIV 113 + + Y+K H+ QL FI L L YH + + + D+ Sbjct: 67 IAL---------YYKEHSSVLKRAEQLHFITQLSAFLYTKHYHLICLNQEYIG-KYDV-- 114 Query: 114 ERKLEAVFLIDAQDASFYSISRHFVEGIPLILIDSMIEDKLFKQV------VYNYRSALE 167 + + ++ FY I +PLI IDS+I+D LF Q+ ++N SA Sbjct: 115 ---ADVILTLEVTKEEFYKIGNS--NFVPLIAIDSIIDDPLFFQINSDPHLLFNKASAYF 169 Query: 168 KALPDDLSQVCLIMESFNNSLLTRYIRSC 196 P +L + + + L ++ C Sbjct: 170 AGEPFELVSLPIQNQEITQGLAQQFDNIC 198 >C0MBU1.1 C0MBU1_STRE4 LacI family regulatory protein Length = 265 Score = 78.2 bits (191), Expect = 8e-13, Method: Compositional matrix adjust. Identities = 64/209 (30%), Positives = 99/209 (47%), Gaps = 30/209 (14%) Query: 1 MKDIAIRANVSVATVSYVLNRVDNQTIPEKTRRQILQLAEELHYIPNLAARSLANQKTGL 60 +KD+A A VSVATVSYV+N ++Q I +TR ++LQ+ L+Y PN AA+SL QK L Sbjct: 7 IKDVAREAGVSVATVSYVINNREDQKISPETRNKVLQMINLLNYRPNQAAKSLVTQKRQL 66 Query: 61 VGVLVHRSRQLPYWKLHA-------QLAFIHTLEQRLTAAGYHTLLYSLDTGNPSLDIIV 113 + + Y+K H+ QL FI L L YH + + + D+ Sbjct: 67 IAL---------YYKEHSSVLKRAEQLHFITQLSAFLYTKHYHLICLNQEYIG-KYDV-- 114 Query: 114 ERKLEAVFLIDAQDASFYSISRHFVEGIPLILIDSMIEDKLFKQV------VYNYRSALE 167 + + ++ FY I +PLI IDS+I+D LF Q+ ++N SA Sbjct: 115 ---ADVILTLEVTKEEFYKIGNS--NFVPLIAIDSIIDDPLFFQINSDPHLLFNKASAYF 169 Query: 168 KALPDDLSQVCLIMESFNNSLLTRYIRSC 196 P +L + + + L ++ C Sbjct: 170 AGEPFELVSLPIQNQEITQGLAQQFDNIC 198 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.136 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,658,008,473 Number of extensions: 64784337 Number of successful extensions: 163261 Number of sequences better than 1.0e-05: 1212 Number of HSP's gapped: 166508 Number of HSP's successfully gapped: 1212 Length of query: 298 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 163 Effective length of database: 1,788,859,178 Effective search space: 291584046014 Effective search space used: 291584046014 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)