BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0255.0005.p (367 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B2D1U4.1 B2D1U4_9BACL Cyclodextrin glucanotransferase 252 3e-65 Q9F5W3.1 Q9F5W3_BACCI Cyclodextrin glucanotransferase 251 9e-65 Q197W1.1 Q197W1_9BACI Beta-cyclodextrin glycosyltransferase EC=2... 251 9e-65 Q6S3E3.1 Q6S3E3_9BACI CGTase 246 3e-63 P43379.1 CDGT2_BACCI Cyclomaltodextrin glucanotransferase EC=2.4... 245 5e-63 >B2D1U4.1 B2D1U4_9BACL Cyclodextrin glucanotransferase Length = 713 Score = 252 bits (644), Expect = 3e-65, Method: Compositional matrix adjust. Identities = 133/333 (39%), Positives = 187/333 (56%), Gaps = 6/333 (1%) Query: 23 GLPVVYYGTEQYMSGGDDPHNRAMMSSFSRETTXXXXVAQLNRLRACNLAIGYGSTKLLY 82 G+P +YYG+EQYMSGG+DP NRA + SFS TT + +L LR N AI YGST+ + Sbjct: 381 GVPAIYYGSEQYMSGGNDPDNRAAIPSFSTTTTAYQVIQKLAPLRKSNPAIAYGSTQERW 440 Query: 83 VNSGVLVFERSCKRDVALILINQEDNAQI-LPSLATSLPQGAYSSLLKDIYPDHPAVAGA 141 +N+ V+++ER +VA++ IN+ N + LATSLPQG+Y+ +L I + GA Sbjct: 441 INNDVIIYERKFGNNVAVVAINRNMNTPASITGLATSLPQGSYNDVLGGILNGNTLTVGA 500 Query: 142 EGDMQNVMLAPRSAFVYSYESDLEKPAAVHVYPKAAAPGSEFIIRGRHLGD-TGCLTVGG 200 G N LAP V+ Y +D P +V P A PG I GR G G + G Sbjct: 501 GGAASNFTLAPGGTAVWQYTTDATAPIIGNVGPMMAKPGVTITIDGRGFGSGKGTVYFGT 560 Query: 201 CE---AAVISWQSGKIVARVPAVPAGVHPVQGLSEGGQKFRLPEELKVWTGMLVTVRLVL 257 A +++W+ +I ++PAVP G++ ++ + G + + +V TG VTVR V+ Sbjct: 561 TAVTGADIVAWEDTQIRVKIPAVPGGIYDIKIANAAGAASNIYDNFEVLTGDQVTVRFVI 620 Query: 258 KNVHTDFDTRVYVTGNVYELGAWQAERAAGPFYNQIVYRYPTWYCDISLPADSRIEFKIM 317 N T V++TGNV ELG W A GP YNQ+VY+YPTWY D+S+PA IEFK + Sbjct: 621 NNATTALGQNVFLTGNVSELGNWDPNNAIGPMYNQVVYQYPTWYYDVSVPAGQTIEFKFL 680 Query: 318 LRSGSGMMRLEQGEAHCFHTPAEGVSEIIAEWQ 350 + GS + E G F TP G + + WQ Sbjct: 681 KKQGS-TVTWEGGANATFTTPTSGTATVNVNWQ 712 >Q9F5W3.1 Q9F5W3_BACCI Cyclodextrin glucanotransferase Length = 713 Score = 251 bits (640), Expect = 9e-65, Method: Compositional matrix adjust. Identities = 132/333 (39%), Positives = 186/333 (55%), Gaps = 6/333 (1%) Query: 23 GLPVVYYGTEQYMSGGDDPHNRAMMSSFSRETTXXXXVAQLNRLRACNLAIGYGSTKLLY 82 G+P +YYG+EQYMSGG+DP NRA + SFS TT + +L LR N AI YGST+ + Sbjct: 381 GVPAIYYGSEQYMSGGNDPDNRARIPSFSTTTTAYQVIQKLAPLRKSNPAIAYGSTQERW 440 Query: 83 VNSGVLVFERSCKRDVALILINQEDNAQI-LPSLATSLPQGAYSSLLKDIYPDHPAVAGA 141 +N+ V+++ER +VA++ IN+ N + L TSLPQG+Y+ +L I + GA Sbjct: 441 INNDVIIYERKFGNNVAVVAINRNMNTPASITGLVTSLPQGSYNDVLGGILNGNTLTVGA 500 Query: 142 EGDMQNVMLAPRSAFVYSYESDLEKPAAVHVYPKAAAPGSEFIIRGRHLGD-TGCLTVGG 200 G N LAP V+ Y +D P +V P A PG I GR G G + G Sbjct: 501 GGAASNFTLAPGGTAVWQYTTDATAPIIGNVGPMMAKPGVTITIDGRGFGSGKGTVYFGT 560 Query: 201 CE---AAVISWQSGKIVARVPAVPAGVHPVQGLSEGGQKFRLPEELKVWTGMLVTVRLVL 257 A +++W+ +I ++PAVP G++ ++ + G + + +V TG VTVR V+ Sbjct: 561 TAVTGADIVAWEDTQIQVKIPAVPGGIYDIRVANAAGAASNIYDNFEVLTGDQVTVRFVI 620 Query: 258 KNVHTDFDTRVYVTGNVYELGAWQAERAAGPFYNQIVYRYPTWYCDISLPADSRIEFKIM 317 N T V++TGNV ELG W A GP YNQ+VY+YPTWY D+S+PA IEFK + Sbjct: 621 NNATTALGQNVFLTGNVSELGNWDPNNAIGPMYNQVVYQYPTWYYDVSVPAGQTIEFKFL 680 Query: 318 LRSGSGMMRLEQGEAHCFHTPAEGVSEIIAEWQ 350 + GS + E G F TP G + + WQ Sbjct: 681 KKQGS-TVTWEGGANRTFTTPTSGTATMNVNWQ 712 >Q197W1.1 Q197W1_9BACI Beta-cyclodextrin glycosyltransferase EC=2.4.1.19 Length = 713 Score = 251 bits (640), Expect = 9e-65, Method: Compositional matrix adjust. Identities = 132/333 (39%), Positives = 186/333 (55%), Gaps = 6/333 (1%) Query: 23 GLPVVYYGTEQYMSGGDDPHNRAMMSSFSRETTXXXXVAQLNRLRACNLAIGYGSTKLLY 82 G+P +YYG+EQYMSGG+DP NRA + SFS TT + +L LR N AI YGST+ + Sbjct: 381 GVPAIYYGSEQYMSGGNDPDNRARIPSFSTTTTAYQVIQKLAPLRKSNPAIAYGSTQERW 440 Query: 83 VNSGVLVFERSCKRDVALILINQEDNAQI-LPSLATSLPQGAYSSLLKDIYPDHPAVAGA 141 +N+ V+++ER +VA++ IN+ N + L TSLPQG+Y+ +L I + GA Sbjct: 441 INNDVIIYERKFGNNVAVVAINRNMNTPASITGLVTSLPQGSYNDVLGGILNGNTLTVGA 500 Query: 142 EGDMQNVMLAPRSAFVYSYESDLEKPAAVHVYPKAAAPGSEFIIRGRHLGD-TGCLTVGG 200 G N LAP V+ Y +D P +V P A PG I GR G G + G Sbjct: 501 GGAASNFTLAPGGTAVWQYTTDATAPIIGNVGPMMAKPGVTITIDGRGFGSGKGTVYFGT 560 Query: 201 CE---AAVISWQSGKIVARVPAVPAGVHPVQGLSEGGQKFRLPEELKVWTGMLVTVRLVL 257 A +++W+ +I ++PAVP G++ ++ + G + + +V TG VTVR V+ Sbjct: 561 TAVTGADIVAWEDTQIQVKIPAVPGGIYDIRVANAAGAASNIYDNFEVLTGDQVTVRFVI 620 Query: 258 KNVHTDFDTRVYVTGNVYELGAWQAERAAGPFYNQIVYRYPTWYCDISLPADSRIEFKIM 317 N T V++TGNV ELG W A GP YNQ+VY+YPTWY D+S+PA IEFK + Sbjct: 621 NNATTALGQNVFLTGNVSELGNWDPNNAIGPMYNQVVYQYPTWYYDVSVPAGQTIEFKFL 680 Query: 318 LRSGSGMMRLEQGEAHCFHTPAEGVSEIIAEWQ 350 + GS + E G F TP G + + WQ Sbjct: 681 KKQGS-TVTWEGGANRTFTTPTSGTATMNVNWQ 712 >Q6S3E3.1 Q6S3E3_9BACI CGTase Length = 712 Score = 246 bits (627), Expect = 3e-63, Method: Compositional matrix adjust. Identities = 129/332 (38%), Positives = 183/332 (55%), Gaps = 5/332 (1%) Query: 23 GLPVVYYGTEQYMSGGDDPHNRAMMSSFSRETTXXXXVAQLNRLRACNLAIGYGSTKLLY 82 G+P +YYG+EQYMSGG+DP NRA + SFS TT + +L LR N AI YGST+ + Sbjct: 381 GVPAIYYGSEQYMSGGNDPDNRARIPSFSTTTTAYQVIQKLAPLRKSNPAIAYGSTQERW 440 Query: 83 VNSGVLVFERSCKRDVALILINQEDNAQI-LPSLATSLPQGAYSSLLKDIYPDHPAVAGA 141 +N+ V+++ER +VA++ IN+ N + L TSLPQG+Y+ +L I + GA Sbjct: 441 INNDVIIYERKFGNNVAVVAINRNMNTPASITGLVTSLPQGSYNDVLGGILNGNTLTVGA 500 Query: 142 EGDMQNVMLAPRSAFVYSYESDLEKPAAVHVYPKAAAPGSEFIIRGRHLGDTGCLTVGGC 201 G N LAP V+ Y +D P +V P A PG I GR G + G Sbjct: 501 GGAASNFTLAPGGTAVWQYTTDATAPIIGNVGPMMAKPGVTITIDGRASARQGTVYFGTT 560 Query: 202 E---AAVISWQSGKIVARVPAVPAGVHPVQGLSEGGQKFRLPEELKVWTGMLVTVRLVLK 258 A +++W+ +I ++ VP G++ ++ + G + + +V TG VTVR V+ Sbjct: 561 AVTGADIVAWEDTQIQVKILRVPGGIYDIRVANAAGAASNIYDNFEVLTGDQVTVRFVIN 620 Query: 259 NVHTDFDTRVYVTGNVYELGAWQAERAAGPFYNQIVYRYPTWYCDISLPADSRIEFKIML 318 N T V++TGNV ELG W A GP YNQ+VY+YPTWY D+S+PA IEFK + Sbjct: 621 NATTALGQNVFLTGNVSELGNWDPNNAIGPMYNQVVYQYPTWYYDVSVPAGQTIEFKFLK 680 Query: 319 RSGSGMMRLEQGEAHCFHTPAEGVSEIIAEWQ 350 + GS + E G F TP G + + WQ Sbjct: 681 KQGS-TVTWEGGANRTFTTPTSGTATVNVNWQ 711 >P43379.1 CDGT2_BACCI Cyclomaltodextrin glucanotransferase EC=2.4.1.19 Length = 713 Score = 245 bits (626), Expect = 5e-63, Method: Compositional matrix adjust. Identities = 130/335 (38%), Positives = 184/335 (54%), Gaps = 10/335 (2%) Query: 23 GLPVVYYGTEQYMSGGDDPHNRAMMSSFSRETTXXXXVAQLNRLRACNLAIGYGSTKLLY 82 G+P +YYGTEQYMSGG DP NRA + SFS TT + +L LR CN AI YGST+ + Sbjct: 381 GVPAIYYGTEQYMSGGTDPDNRARIPSFSTSTTAYQVIQKLAPLRKCNPAIAYGSTQERW 440 Query: 83 VNSGVLVFERSCKRDVALILINQEDNAQI-LPSLATSLPQGAYSSLLKDIYPDHPAVAGA 141 +N+ VL++ER +VA++ +N+ NA + L TSLPQG+Y+ +L + + G+ Sbjct: 441 INNDVLIYERKFGSNVAVVAVNRNLNAPASISGLVTSLPQGSYNDVLGGLLNGNTLSVGS 500 Query: 142 EGDMQNVMLAPRSAFVYSYESDLEKPAAVHVYPKAAAPGSEFIIRGRHLGDT------GC 195 G N LA V+ Y + P HV P A PG I GR G + G Sbjct: 501 GGAASNFTLAAGGTAVWQYTAATATPTIGHVGPMMAKPGVTITIDGRGFGSSKGTVYFGT 560 Query: 196 LTVGGCEAAVISWQSGKIVARVPAVPAGVHPVQGLSEGGQKFRLPEELKVWTGMLVTVRL 255 V G + + SW+ +I ++PAV G + ++ + G + + +V +G V+VR Sbjct: 561 TAVSGAD--ITSWEDTQIKVKIPAVAGGNYNIKVANAAGTASNVYDNFEVLSGDQVSVRF 618 Query: 256 VLKNVHTDFDTRVYVTGNVYELGAWQAERAAGPFYNQIVYRYPTWYCDISLPADSRIEFK 315 V+ N T VY+TG+V ELG W +A GP YNQ+VY+YP WY D+S+PA IEFK Sbjct: 619 VVNNATTALGQNVYLTGSVSELGNWDPAKAIGPMYNQVVYQYPNWYYDVSVPAGKTIEFK 678 Query: 316 IMLRSGSGMMRLEQGEAHCFHTPAEGVSEIIAEWQ 350 + + GS + E G H F P+ G + I WQ Sbjct: 679 FLKKQGS-TVTWEGGSNHTFTAPSSGTATINVNWQ 712 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.136 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,307,611,186 Number of extensions: 95970833 Number of successful extensions: 184831 Number of sequences better than 1.0e-05: 163 Number of HSP's gapped: 184872 Number of HSP's successfully gapped: 163 Length of query: 367 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 230 Effective length of database: 1,770,017,148 Effective search space: 407103944040 Effective search space used: 407103944040 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)