BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0255.0002.p
         (382 letters)

Database: pfamseq 
           9,421,015 sequences; 3,060,696,203 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Q4K7P6.1 Q4K7P6_PSEF5 Coenzyme F420-reducing hydrogenase, beta s...   194   9e-48
Q3ACD9.1 Q3ACD9_CARHZ Coenzyme F420 hydrogenase, beta subunit         155   4e-36
A5D272.1 A5D272_PELTS Coenzyme F420-reducing hydrogenase, beta s...   150   3e-34
A5FSQ6.1 A5FSQ6_DEHSB Coenzyme F420 hydrogenase/dehydrogenase be...   110   2e-22
O26441.1 O26441_METTH Coenzyme F420-reducing hydrogenase, beta s...   109   4e-22

>Q4K7P6.1 Q4K7P6_PSEF5 Coenzyme F420-reducing hydrogenase, beta
           subunit-related protein
          Length = 402

 Score =  194 bits (494), Expect = 9e-48,   Method: Compositional matrix adjust.
 Identities = 120/352 (34%), Positives = 180/352 (51%), Gaps = 18/352 (5%)

Query: 8   LPVFQGLESQAEDTXXXXGSCNLCSEVCPGYDTGVMESERRIFGRNRSELERWTGIYLST 67
           +P+ +G+    E       SC  C  VCPG       SE  +FGR R   ERW GI    
Sbjct: 36  VPLLEGIGDSCE-------SCTECLAVCPGLQPDTDRSELELFGRTRRAEERWLGISRQA 88

Query: 68  HQFSAADPEILGRAAAGGAGTILAVTALEEKLADAVIVVGRDEERPWVPKAYLADSVERI 127
               + DP+I+ R+A+GG  T L +TA E    D  + +GR   + W     L  +   I
Sbjct: 89  IGARSTDPQIVRRSASGGTATTLLLTAREHFDVDLTLTMGRHPGQGWRSAPQLGSAAAAI 148

Query: 128 IECAQTSYCITPNLDLLQDDRYD----KIGIIGVPCQIQGIHKLLNLPEHLPSSVLADKV 183
           I  AQ++Y + P L+ L++        +I ++G+ C +Q I KL      +     + ++
Sbjct: 149 INNAQSTYQLAPYLNALRECYEQQPDARIAVVGLACHVQAIRKLQRRDTAIGHWARS-RI 207

Query: 184 AFTIELGCASSTSLGGTEHLITEILGIELADVAVMRYREGQYPGQFMVKTRQGQEHYLPF 243
              IE  C+S+T   GT  +I+E+LG +   V  ++YREG YPG+  V     Q   + F
Sbjct: 208 ICVIETACSSNTLPAGTHSIISEVLGQDPQQVRDIKYREGDYPGRLQVTLLDSQTFGIDF 267

Query: 244 YRLVEEFKKFKTFRCLACPDWWSGIADISISDGDPNIFDSSKEGISAKASSTVMVRTKTG 303
           + ++ + K  KTFRCL+C DW SG+AD+S+ DGDPNIF++S  G   +     ++RT+ G
Sbjct: 268 WEILGKLKDNKTFRCLSCGDWMSGLADVSVCDGDPNIFEASLNGSEEQKHGRALIRTEMG 327

Query: 304 ARLLELAVRRNAARLVDYTFD---NNLGLERKRQRYRSYAAMGDRRIPLAPG 352
             L++ A+     RL  +  D    NLGLERK+ R R Y       +P  PG
Sbjct: 328 QALVDSAI--THGRLESWGVDLGGFNLGLERKKNRRRFYETQ-PLVLPAGPG 376


>Q3ACD9.1 Q3ACD9_CARHZ Coenzyme F420 hydrogenase, beta subunit
          Length = 400

 Score =  155 bits (393), Expect = 4e-36,   Method: Compositional matrix adjust.
 Identities = 95/299 (31%), Positives = 155/299 (51%), Gaps = 17/299 (5%)

Query: 28  CNLCSEVCPGYDTGVMESERRIFGRNRSELERWTGIYLSTHQFSAADPEILGRAAAGGAG 87
           C +C  +CPG D  ++E E+  FG NR+      G Y  ++   A +  I  + A+GG  
Sbjct: 53  CGICFRICPGSDVPLLEFEKHFFGSNRNPTLTDLGFYRGSYVGYAGEEIIRIKGASGGVV 112

Query: 88  TILAVTALEEKLADAVIVVGRDEERPWVPKAYLADSVERIIECAQTSYCITPNLDLLQDD 147
           T L V ALE+ + D  IV G ++E+PW+ +A LA S + II+ AQ+ Y + P   L ++ 
Sbjct: 113 TALLVYALEKGIIDCAIVAGFNKEKPWLTEAKLAKSKKEIIDAAQSKYAVVPVNMLFREA 172

Query: 148 RY---DKIGIIGVPCQIQGIHKL--LNLPEHLPSSVLADKVAFTIELGCASSTSLGGTEH 202
                 ++GI+G+PC I  + K+   ++P       +A K+   I L CAS     GT H
Sbjct: 173 VKAGCQRVGIVGLPCHIHAVRKMQYYDIPRD-----IAGKIKLAIGLFCASQFYFEGTRH 227

Query: 203 LITEILGI-ELADVAVMRYREGQYPGQFMVKTRQGQEHYLPFYRLVEE--FKKFKTFRCL 259
           ++ E  G+ ++ ++A + YR G++PG F+VK    +   +  +R V       +K  RC 
Sbjct: 228 ILVEWCGVDDMEEIANLEYRGGEWPGHFVVKLHSDKTITIDRHRYVYHMLMPNYKRDRCE 287

Query: 260 ACPDWWSGIADISISDGDPNIFDSSKEGISAKASSTVMVRTKTGARLLELAVRRNAARL 318
            C DW S +ADI++ D     + S   G +   +S ++VR+  G  L++ AV      L
Sbjct: 288 MCIDWSSELADIAVGD----YWASPTIGDNFSGTSALLVRSLPGEELIKSAVEEKVLYL 342


>A5D272.1 A5D272_PELTS Coenzyme F420-reducing hydrogenase, beta subunit
          Length = 404

 Score =  150 bits (378), Expect = 3e-34,   Method: Compositional matrix adjust.
 Identities = 107/320 (33%), Positives = 155/320 (48%), Gaps = 22/320 (6%)

Query: 27  SCNLCSEVCPGYDTGVMESERRIFGRNRSELERWTGIYLSTHQFSAADPEILGRAAAGGA 86
           +C +C  VCPG +  +   E+ IFGR R E     G Y       +AD  +  R A+GG 
Sbjct: 52  NCGICPAVCPGAEVNIPVLEKFIFGRERPEEIPDLGFYREALLAWSADLPVRKRGASGGL 111

Query: 87  GTILAVTALEEKLADAVIVVGRDEERPWVPKAYLADSVERIIECAQTSYCITPNLDLLQ- 145
            T + V ALEE + D  +V G   + P    A+LA S E I+  AQ+ Y   P   LL  
Sbjct: 112 VTSVLVYALEEGIIDCALVAGFRRDDPCRTGAWLAASKEDILAAAQSKYACVPVNTLLSR 171

Query: 146 --DDRYDKIGIIGVPCQIQGIHKLLNLPEHLPSSVLADKVAFTIELGCASSTSLGGTEHL 203
                Y +I  +G+PCQI G+ K+  L    P+S +A  + F   L CA+     GT HL
Sbjct: 172 ALAQGYRRIAAVGLPCQIHGLRKMQYLGR--PAS-MAKSIVFMTGLFCAAQFYFEGTRHL 228

Query: 204 ITEILGIE-LADVAVMRYREGQYPGQFMVKTRQGQ-----EHYLPFYRLVEEFKKFKTFR 257
           I E LG+  L ++A M YR G +PG  +V+ + G+      H   ++ L+  FK+    R
Sbjct: 229 IVERLGLTGLDEIAAMSYRGGDWPGHLVVELKDGRRLLLDRHQYMYHHLMPAFKR---DR 285

Query: 258 CLACPDWWSGIADISISDGDPNIFDSSKEGISAKASSTVMVRTKTGARLLELAVRRN--- 314
           C  C DW + +AD+S+ D     +D       A  +S+ +VRT  G  +L+ AV+     
Sbjct: 286 CEMCVDWSAELADLSVGD----YWDPGARAGEALGTSSCLVRTAAGEEVLKGAVQSGYLE 341

Query: 315 AARLVDYTFDNNLGLERKRQ 334
            A L        LG E K+ 
Sbjct: 342 TAGLEARRLAAGLGFELKKH 361


>A5FSQ6.1 A5FSQ6_DEHSB Coenzyme F420 hydrogenase/dehydrogenase beta subunit
           domain protein
          Length = 428

 Score =  110 bits (276), Expect = 2e-22,   Method: Compositional matrix adjust.
 Identities = 93/363 (25%), Positives = 155/363 (42%), Gaps = 30/363 (8%)

Query: 27  SCNLCSEVCPGYDTGVMESERRIFGRNRSELERWTGIYLSTHQFSAADPEILGRAAAGGA 86
           SC  C +VCPG    +    + IFG     L     I  S +   ++D      + +GG 
Sbjct: 56  SCGKCLKVCPGTGIDLDSYSQAIFGGASPSL-----IQNSCYTAFSSDHLQRNLSTSGGV 110

Query: 87  GTILAVTALEEKLADAVIVVGRDEERPWVPKAYLADSVERIIECAQTSYCITPNLDLLQD 146
            T L   ALE  L D  +V G +   P  P +++A + E + + A + YC       + +
Sbjct: 111 ATALLNYALETGLIDGALVSGANPSSPLTPASFIARTPEELKQAAGSKYCPVAAASAISE 170

Query: 147 --DRYDKIGIIGVPCQIQGIHKLLNLPEHLPSSVLADKVAFTIELGCASSTSLGGTEHLI 204
              +  +  ++G+PC IQ I K  N      + +L +++   + + C+S+ S  GTE+++
Sbjct: 171 ITAKPGRYAVVGLPCHIQAIRKAEN-----QNPLLKERIVLHLGIVCSSTMSFRGTEYIL 225

Query: 205 TEILGIELADVAVMRYREGQYPGQFMVKTRQGQEHYLPF--YRLVEEFKKFKTFRCLACP 262
            +  GI   ++  + YR G +PG   ++   G    LP   Y        F   RCL C 
Sbjct: 226 KQ-QGINKINLISLHYRTGGWPGDMHLEMACGDTQTLPLRNYHPYHGHGFFSPRRCLMCT 284

Query: 263 DWWSGIADISISDGDPNIFDSSKEGISAKASSTVMVRTKTGARLLELAVRRNAARLVDYT 322
           D  + +ADIS+ D      +   EG SA     V+VR  TG      AV+    +    +
Sbjct: 285 DQLNELADISLGDAWLRELEYDHEGTSA-----VIVRGDTGEDFYRNAVQYGCIKSQPLS 339

Query: 323 FDNNLGLERKRQRY---RSYAAMGDRRIP-----LAPGRDMDYSQILSDDEVIRIGIGPK 374
               +    KRQ Y   ++  ++   ++P       P R + Y   L  ++VI   +G  
Sbjct: 340 NKRVMEWRNKRQDYAAKKNILSLLGWKLPEYSHSCPPARKIAYLYTL--EQVINYHLGKT 397

Query: 375 QGR 377
             R
Sbjct: 398 NSR 400


>O26441.1 O26441_METTH Coenzyme F420-reducing hydrogenase, beta subunit
           homolog
          Length = 406

 Score =  109 bits (273), Expect = 4e-22,   Method: Compositional matrix adjust.
 Identities = 73/250 (29%), Positives = 120/250 (48%), Gaps = 10/250 (4%)

Query: 28  CNLCSEVCPGYDTGVMESERRIFGRNRSELERWTGIYLSTHQFSAADPEILGRAAAGGAG 87
           C +C  VCPG      +   R+FGR   ++    G Y   +   + D E+   A++GG  
Sbjct: 48  CGVCLRVCPGVGVDFTDLNMRVFGREPDDM--LMGNYEECYIAHSRDDELRYGASSGGMI 105

Query: 88  TILAVTALEEKLADAVIVVGRDEERPWVPKAYLADSVERIIECAQTSYCITPNLDLLQD- 146
           + + +  LEE L D  +V   + ERP  P+ ++A +   IIE   + YC  P    L++ 
Sbjct: 106 SQILIYLLEEGLIDGALVTRMNPERPLEPEPFIARTPSEIIESRGSKYCPVPANVALKEI 165

Query: 147 -DRYDKIGIIGVPCQIQGIHKLLNLPEHLPSSVLADKVAFTIELGCASSTSLGGTEHLIT 205
            +   +  ++G+PC I G+ K       + S  L +++ + + + C  + S   TE L+ 
Sbjct: 166 LEVPGRYAVVGLPCHIHGVRK-----AEVISRKLRERIVYHLGIVCNHAPSFRATEFLL- 219

Query: 206 EILGIELADVAVMRYREGQYPGQFMVKTRQGQEHYLPFYRLVEEFKKFKTFRCLACPDWW 265
           E LG++  DV  +RYR   +PG   V+T  G    LP Y      + F   RC  C D  
Sbjct: 220 ERLGVDPGDVKSIRYRGEGWPGSLRVETESGDLLLLPEYWGSGFGQLFMPERCRLCADHM 279

Query: 266 SGIADISISD 275
           + ++DIS +D
Sbjct: 280 AELSDISFAD 289


  Database: pfamseq
    Posted date:  Aug 5, 2010  3:22 PM
  Number of letters in database: 3,060,696,203
  Number of sequences in database:  9,421,015
  
Lambda     K      H
   0.319    0.137    0.407 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 9421015
Number of Hits to DB: 2,253,948,096
Number of extensions: 91621699
Number of successful extensions: 202136
Number of sequences better than 1.0e-05: 115
Number of HSP's gapped: 202026
Number of HSP's successfully gapped: 115
Length of query: 382
Length of database: 3,060,696,203
Length adjustment: 137
Effective length of query: 245
Effective length of database: 1,770,017,148
Effective search space: 433654201260
Effective search space used: 433654201260
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 132 (55.5 bits)