BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0254.0032.p (339 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C0Z5U2.1 C0Z5U2_BREBN Conserved hypothetical membrane protein 152 6e-35 C2PYS4.1 C2PYS4_BACCE Major facilitator superfamily MFS_1 128 9e-28 C3BV86.1 C3BV86_9BACI Major facilitator superfamily MFS_1 115 4e-24 C3BCM3.1 C3BCM3_BACMY Major facilitator superfamily MFS_1 115 4e-24 C3AV88.1 C3AV88_BACMY Major facilitator superfamily MFS_1 115 5e-24 >C0Z5U2.1 C0Z5U2_BREBN Conserved hypothetical membrane protein Length = 418 Score = 152 bits (383), Expect = 6e-35, Method: Compositional matrix adjust. Identities = 92/287 (32%), Positives = 139/287 (48%), Gaps = 2/287 (0%) Query: 17 PNMVWXXXXXXXXXXXXXXNVPAQQSMTRSIVREDQLLQATSLNGIVNQGSKIAGPLLGG 76 PN W N PAQQ++TR IV ++QLLQA++LNG V Q K+ GPL GG Sbjct: 93 PNPFWFLVVIALRSGASVFNDPAQQTLTRQIVPKNQLLQASALNGAVYQMGKLIGPLAGG 152 Query: 77 VALTLLSPQWCILLNAIFRVGSYLLLLSVKNIHTGDEHPAAEVQEERVPLRKMWAEGWSF 136 + + +P C+++NA + S L +++ + P+ V+E+ +P R+ W EGW Sbjct: 153 LVAAVFAPAICLMINASTFLLSTGFLFTIRKVEKTLSRPS--VEEQPLPYRQAWQEGWRI 210 Query: 137 MLRSRLLLHTMLFGLTGALAIQIIDFQFTSLFRVISPANESLLGWMXXXXXXXXXXXILL 196 L++R+LL++ +F + +LAIQ+ D QF +FR P N +LG+ + Sbjct: 211 FLQNRVLLYSTIFAIITSLAIQLADTQFPVIFREKLPDNPEMLGFTVSVIGLGALVTVTW 270 Query: 197 LGKLKPGTSYGLRLXXXXXXXXXXXXXXXXXXXXASVIWVXXXXXXXXXXXXXXXVAFNY 256 L +LK SYG L + W+ V NY Sbjct: 271 LHRLKEIRSYGWVLGGGVLLIGISFSWIGFFQPGDGMYWLLIAALLAGVGTGLTSVGANY 330 Query: 257 CLQKETPPHMTGRIFGIQSSILGLVMITAPLLGGWLVQVAGPGRIFM 303 +QKE+PP GR+ GI S+ I APLLGG ++ + GP F+ Sbjct: 331 LVQKESPPQALGRVRGIIDSLTSATFIIAPLLGGVIMTIWGPNTAFL 377 >C2PYS4.1 C2PYS4_BACCE Major facilitator superfamily MFS_1 Length = 419 Score = 128 bits (321), Expect = 9e-28, Method: Compositional matrix adjust. Identities = 82/288 (28%), Positives = 137/288 (47%), Gaps = 3/288 (1%) Query: 17 PNMVWXXXXXXXXXXXXXXNVPAQQSMTRSIVREDQLLQATSLNGIVNQGSKIAGPLLGG 76 PN+ W PA Q++T+ +V E L QATS NG+++Q +IAGPLLG Sbjct: 98 PNVYWLLLLLAVRSTFASFFQPAHQALTKQVVHEHHLAQATSWNGLMDQVGRIAGPLLGA 157 Query: 77 VALTLLSPQWCILLNAIFRVGSYLLLLSVKNIHTGDEHPAAEVQE-ERVPLRKMWAEGWS 135 + +T LSPQ + + + + S +LLS+ + D+ ++Q+ ER W EGW Sbjct: 158 MLITGLSPQISMSVRGVSSLCSAFILLSISKM-VKDKKQFEQIQKTERQGFFSAWLEGWK 216 Query: 136 FMLRSRLLLHTMLFGLTGALAIQIIDFQFTSLFRVISPANESLLGWMXXXXXXXXXXXIL 195 ++ +++ +TM +GL G +Q+++ Q L R + P + ++GW+ +L Sbjct: 217 YVWNRKIIFYTMFYGLCGVGILQLVESQVPVLMRELFPNSPEMVGWINAIVGLGGVCGVL 276 Query: 196 LLGKLKPGTSYGLRLXXXXXXXXXXXXXXXXXXXXASVIWVXXXXXXXXXXXXXXXVAFN 255 L+ ++ YG L V + ++FN Sbjct: 277 LIKRIGE-LKYGWFLGGGIALIGLGFGSLGLFTETTPVFFTFVIGFIAGIGSGLFFISFN 335 Query: 256 YCLQKETPPHMTGRIFGIQSSILGLVMITAPLLGGWLVQVAGPGRIFM 303 LQKET GR+FGIQSS+ L+ I PL+GG V++ G ++++ Sbjct: 336 IILQKETDIETAGRVFGIQSSLSNLMFIVPPLIGGVFVKLIGVSQVYV 383 >C3BV86.1 C3BV86_9BACI Major facilitator superfamily MFS_1 Length = 420 Score = 115 bits (289), Expect = 4e-24, Method: Compositional matrix adjust. Identities = 81/269 (30%), Positives = 137/269 (50%), Gaps = 6/269 (2%) Query: 38 PAQQSMTRSIVREDQLLQATSLNGIVNQGSKIAGPLLGGVALTLLSPQWCILLNAIFRVG 97 PAQQ++TR IV D L +A S+NGIV QG+KI GPL+GGV L+ P++C+++ AI + Sbjct: 121 PAQQTLTRQIVSPDLLTKAVSINGIVEQGTKIVGPLIGGVLLSWFQPEFCLVIRAICSLL 180 Query: 98 SYLLLLSVKNIHTGDEHPAAEVQEERVPLRKMWAEGWSFMLRSRLLLHTMLFGLTGALAI 157 + L+LL I + +++++ W +GWS++L +R++L TM+F G + Sbjct: 181 AALVLL--PTIRFKESLSREYIEKQQQSTWTAWLQGWSYVLSNRMILSTMIFFTIGMAVL 238 Query: 158 QIIDFQFTSLFRVISPANESLLGWMXXXXXXXXXXXILLLGKLKPGTSYGLRLXXXXXXX 217 Q++D QF +LF+ + P ++S +G++ L K+K YG + Sbjct: 239 QLVDSQFPTLFKSLFPNDKSKMGYIISIIGIGGILGAFLTQKIKH-FQYGWVVCGGIALM 297 Query: 218 XXXXXXXXXXXXXA---SVIWVXXXXXXXXXXXXXXXVAFNYCLQKETPPHMTGRIFGIQ 274 + S+I+ V+ LQ E+ GR+FGIQ Sbjct: 298 GIGFGGISLISLVSPGTSLIFGYLISFIAGIGSGLMLVSNQVILQIESHQEQVGRVFGIQ 357 Query: 275 SSILGLVMITAPLLGGWLVQVAGPGRIFM 303 SS+ V+I +P + G LV + G ++++ Sbjct: 358 SSLANAVLIISPAMSGPLVHLFGVIQLYL 386 >C3BCM3.1 C3BCM3_BACMY Major facilitator superfamily MFS_1 Length = 420 Score = 115 bits (289), Expect = 4e-24, Method: Compositional matrix adjust. Identities = 81/269 (30%), Positives = 137/269 (50%), Gaps = 6/269 (2%) Query: 38 PAQQSMTRSIVREDQLLQATSLNGIVNQGSKIAGPLLGGVALTLLSPQWCILLNAIFRVG 97 PAQQ++TR IV D L +A S+NGIV QG+KI GPL+GGV L+ P++C+++ AI + Sbjct: 121 PAQQTLTRQIVSPDLLTKAVSINGIVEQGTKIVGPLIGGVLLSWFQPEFCLVIRAICSLL 180 Query: 98 SYLLLLSVKNIHTGDEHPAAEVQEERVPLRKMWAEGWSFMLRSRLLLHTMLFGLTGALAI 157 + L+LL I + +++++ W +GWS++L +R++L TM+F G + Sbjct: 181 AALVLL--PTIRFKESLSREYIEKQQQSTWTAWLQGWSYVLSNRMILSTMIFFTIGMAVL 238 Query: 158 QIIDFQFTSLFRVISPANESLLGWMXXXXXXXXXXXILLLGKLKPGTSYGLRLXXXXXXX 217 Q++D QF +LF+ + P ++S +G++ L K+K YG + Sbjct: 239 QLVDSQFPTLFKSLFPNDKSKMGYIISIIGIGGILGAFLTQKIKH-FQYGWVVCGGIALM 297 Query: 218 XXXXXXXXXXXXXA---SVIWVXXXXXXXXXXXXXXXVAFNYCLQKETPPHMTGRIFGIQ 274 + S+I+ V+ LQ E+ GR+FGIQ Sbjct: 298 GIGFGGISLISLVSPGTSLIFGYLISFIAGIGSGLMLVSNQVILQIESHQEQVGRVFGIQ 357 Query: 275 SSILGLVMITAPLLGGWLVQVAGPGRIFM 303 SS+ V+I +P + G LV + G ++++ Sbjct: 358 SSLANAVLIISPAMSGPLVHLFGVIQLYL 386 >C3AV88.1 C3AV88_BACMY Major facilitator superfamily MFS_1 Length = 420 Score = 115 bits (289), Expect = 5e-24, Method: Compositional matrix adjust. Identities = 81/269 (30%), Positives = 137/269 (50%), Gaps = 6/269 (2%) Query: 38 PAQQSMTRSIVREDQLLQATSLNGIVNQGSKIAGPLLGGVALTLLSPQWCILLNAIFRVG 97 PAQQ++TR IV D L +A S+NGIV QG+KI GPL+GGV L+ P++C+++ AI + Sbjct: 121 PAQQTLTRQIVSPDLLTKAVSINGIVEQGTKIVGPLIGGVLLSWFQPEFCLVIRAICSLL 180 Query: 98 SYLLLLSVKNIHTGDEHPAAEVQEERVPLRKMWAEGWSFMLRSRLLLHTMLFGLTGALAI 157 + L+LL I + +++++ W +GWS++L +R++L TM+F G + Sbjct: 181 AALVLL--PTIRFKESLSREYIEKQQQSTWTAWLQGWSYVLSNRMILSTMIFFTIGMAVL 238 Query: 158 QIIDFQFTSLFRVISPANESLLGWMXXXXXXXXXXXILLLGKLKPGTSYGLRLXXXXXXX 217 Q++D QF +LF+ + P ++S +G++ L K+K YG + Sbjct: 239 QLVDSQFPTLFKSLFPNDKSKMGYIISIIGIGGILGAFLTQKIKH-FQYGWVVCGGIALM 297 Query: 218 XXXXXXXXXXXXXA---SVIWVXXXXXXXXXXXXXXXVAFNYCLQKETPPHMTGRIFGIQ 274 + S+I+ V+ LQ E+ GR+FGIQ Sbjct: 298 GIGFGGISLISLVSPGTSLIFGYLISFIAGIGSGLMLVSNQVILQIESHQEQVGRVFGIQ 357 Query: 275 SSILGLVMITAPLLGGWLVQVAGPGRIFM 303 SS+ V+I +P + G LV + G ++++ Sbjct: 358 SSLANAVLIISPAMSGPLVHLFGVIQLYL 386 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.324 0.139 0.434 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,377,331,337 Number of extensions: 44930736 Number of successful extensions: 135501 Number of sequences better than 1.0e-05: 41 Number of HSP's gapped: 135552 Number of HSP's successfully gapped: 41 Length of query: 339 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 203 Effective length of database: 1,779,438,163 Effective search space: 361225947089 Effective search space used: 361225947089 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 131 (55.1 bits)