BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0254.0028.p (259 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A3IFR4.1 A3IFR4_9BACI Sec-independent secretion TatD 272 3e-71 B3K4F5.1 B3K4F5_9BACI TatD-related deoxyribonuclease 270 6e-71 B1DJN3.1 B1DJN3_9BACL TatD-related deoxyribonuclease 260 7e-68 Q2B458.1 Q2B458_9BACI TatD-related deoxyribonuclease 233 1e-59 A6CPC2.1 A6CPC2_9BACI Deoxyribonuclease, TatD family, putative 226 2e-57 >A3IFR4.1 A3IFR4_9BACI Sec-independent secretion TatD Length = 249 Score = 272 bits (695), Expect = 3e-71, Method: Compositional matrix adjust. Identities = 134/259 (51%), Positives = 170/259 (65%), Gaps = 11/259 (4%) Query: 1 MIDAHIHLEQYEDSELQAMLPGLLEQDIQSLVAVSMNLDSCIRTQEIAARYPGRVKPAYG 60 +IDAHIHL+QY+D E+ P LLE ++++L+AVSM L SC +T +A YP +VK A+G Sbjct: 2 LIDAHIHLDQYKDVEI----PSLLE-EVEALIAVSMQLSSCQKTLNLAKVYP-KVKAAFG 55 Query: 61 FHPEQPAPTESELAALLKWISQHAGEMVAVGEIGLPYYSRAEALARGEAFEQEPYXXXXX 120 FHPEQP P+E E AL WI H+ EM+AVGE+GLPYY R E +A + PY Sbjct: 56 FHPEQPLPSEIEEKALFDWIRSHSDEMIAVGEVGLPYYLRQE-----QALDVRPYEALLE 110 Query: 121 XXXXXXXXXXKPVVLHAVYEDAITACNLLEKHGIARAHFHWFKGPEEAVTRMIERGYHIS 180 KP+VLHAVYEDA C LLEKH + +AHFHWFKG E V RMI++GY IS Sbjct: 111 RFIVLAKELDKPIVLHAVYEDATVVCELLEKHQLRQAHFHWFKGDEAVVKRMIQQGYFIS 170 Query: 181 FTPDIVYEPEIRELARRYPPGLVMAETDGPWPFEGPFAGRPTRPAMVHDVAAAWAELHGY 240 TPD +YE EI++L + YP L+M ETDGPWPFEGPF +PT P M+H A + G Sbjct: 171 VTPDALYEEEIQQLIQIYPIELMMVETDGPWPFEGPFTNKPTSPWMMHSTIEVIAAIKGL 230 Query: 241 SAAQAKAMLTANTARFYQL 259 S +A ++T NT FY+L Sbjct: 231 SIQEAAQIITQNTTNFYRL 249 >B3K4F5.1 B3K4F5_9BACI TatD-related deoxyribonuclease Length = 271 Score = 270 bits (691), Expect = 6e-71, Method: Compositional matrix adjust. Identities = 127/259 (49%), Positives = 174/259 (67%) Query: 1 MIDAHIHLEQYEDSELQAMLPGLLEQDIQSLVAVSMNLDSCIRTQEIAARYPGRVKPAYG 60 ++D HIHL+ Y S+ Q +L + + +++++++VSMNL S R E+A Y V+PA+G Sbjct: 13 LLDTHIHLDSYAFSDQQTILADIRKSEVKTIISVSMNLASSKRNLELAREYGPLVRPAFG 72 Query: 61 FHPEQPAPTESELAALLKWISQHAGEMVAVGEIGLPYYSRAEALARGEAFEQEPYXXXXX 120 +HPEQP P+ E LL W++ H +M+AVGE+GLPYY+R EA G ++ PY Sbjct: 73 YHPEQPVPSPEETKELLDWMANHVDDMIAVGEVGLPYYARLEAETEGRPWDNRPYECLLE 132 Query: 121 XXXXXXXXXXKPVVLHAVYEDAITACNLLEKHGIARAHFHWFKGPEEAVTRMIERGYHIS 180 KPV+LHAVYEDA TAC+LL+K+G+ RAHFHWFKG + + RM ++G +IS Sbjct: 133 QFIAFAKRHHKPVILHAVYEDADTACDLLDKYGVTRAHFHWFKGSRDTLQRMADKGCYIS 192 Query: 181 FTPDIVYEPEIRELARRYPPGLVMAETDGPWPFEGPFAGRPTRPAMVHDVAAAWAELHGY 240 FTPDI+YE EIR++AR YP LVM ETDGPWPF+GPF G+ T P M AAAWAEL G Sbjct: 193 FTPDILYEDEIRDIARMYPEHLVMTETDGPWPFDGPFTGQMTHPKMTASAAAAWAELRGL 252 Query: 241 SAAQAKAMLTANTARFYQL 259 + ++A+ + N R Y L Sbjct: 253 TVSEARRIFYENAVRLYDL 271 >B1DJN3.1 B1DJN3_9BACL TatD-related deoxyribonuclease Length = 266 Score = 260 bits (665), Expect = 7e-68, Method: Compositional matrix adjust. Identities = 126/260 (48%), Positives = 165/260 (63%), Gaps = 2/260 (0%) Query: 2 IDAHIHLEQYEDSELQAMLPGLLEQDIQSLVAVSMNLDSCIRTQEIAARYPGRVKPAYGF 61 IDAHIH++ YE+++ QA L E I+++VAVSM+LDSC +A +PG+V PAYG+ Sbjct: 7 IDAHIHIDSYEEADRQAFLEQAFEAGIEAVVAVSMHLDSCKTNASLAELFPGKVFPAYGY 66 Query: 62 HPEQPAPTESELAALLKWI-SQH-AGEMVAVGEIGLPYYSRAEALARGEAFEQEPYXXXX 119 HPEQP PT E L+ W+ ++H AGE A+GE+GLPYYSR A G F++ PY Sbjct: 67 HPEQPVPTAWEQDELVAWVKAKHGAGEAFAIGEVGLPYYSRTSAAEEGRPFDETPYLDLL 126 Query: 120 XXXXXXXXXXXKPVVLHAVYEDAITACNLLEKHGIARAHFHWFKGPEEAVTRMIERGYHI 179 +P++LHAVYED AC+++E+H + + HFHWFKG E + RMI GY I Sbjct: 127 DRFAALAAELGRPIILHAVYEDGAKACDIMERHQLKKVHFHWFKGDEATIKRMISLGYMI 186 Query: 180 SFTPDIVYEPEIRELARRYPPGLVMAETDGPWPFEGPFAGRPTRPAMVHDVAAAWAELHG 239 S TPD+ YE EIR L RYP +M ETDGPWPFEGP+ GR T P MV+DV A A + Sbjct: 187 SVTPDVAYEQEIRTLVERYPLEQMMVETDGPWPFEGPYEGRMTVPQMVYDVCAHIAAIKQ 246 Query: 240 YSAAQAKAMLTANTARFYQL 259 + + L +NT FY L Sbjct: 247 LPEQEVRERLLSNTREFYSL 266 >Q2B458.1 Q2B458_9BACI TatD-related deoxyribonuclease Length = 272 Score = 233 bits (594), Expect = 1e-59, Method: Compositional matrix adjust. Identities = 117/260 (45%), Positives = 161/260 (61%), Gaps = 8/260 (3%) Query: 1 MIDAHIHLEQYEDSELQAMLPGLLEQDIQSLVAVSMNLDSCIRTQEIAARYPGRVKPAYG 60 +ID+HIHL++Y D ++ + Q+++SL+ VS +L SC + +A R+ +VKPA+G Sbjct: 20 IIDSHIHLDRYADQDIANICSI---QELESLITVSWDLSSCKKNLLLAERF-SKVKPAFG 75 Query: 61 FHPEQPAPTESELAALLKWISQHAGEMVAVGEIGLPYYSRAEALARGEAF-EQEPYXXXX 119 +HPEQ P E+ELA L+ W+ H M+A+GE+GLPYY R E +G +F EPY Sbjct: 76 YHPEQVPPPEAELADLIGWMGDHKENMIAIGEVGLPYYLRQE---QGASFIGLEPYLEIL 132 Query: 120 XXXXXXXXXXXKPVVLHAVYEDAITACNLLEKHGIARAHFHWFKGPEEAVTRMIERGYHI 179 PV+LH++YEDA C+LLEK+ I +AHFHWFKG RM GY I Sbjct: 133 ELFVQKAKEWQLPVILHSIYEDAEIVCSLLEKYSIEKAHFHWFKGSALTAERMAANGYFI 192 Query: 180 SFTPDIVYEPEIRELARRYPPGLVMAETDGPWPFEGPFAGRPTRPAMVHDVAAAWAELHG 239 S TPD++YE EI+ L + YP VMAETDGPWPFEGPF + T P M+H AA AE+ Sbjct: 193 SVTPDVLYEGEIQSLVKDYPIRQVMAETDGPWPFEGPFNKQMTSPLMIHRSAAKIAEIKK 252 Query: 240 YSAAQAKAMLTANTARFYQL 259 + + +L NT FY++ Sbjct: 253 MALPEVYTLLLTNTRDFYRI 272 >A6CPC2.1 A6CPC2_9BACI Deoxyribonuclease, TatD family, putative Length = 268 Score = 226 bits (576), Expect = 2e-57, Method: Compositional matrix adjust. Identities = 106/261 (40%), Positives = 156/261 (59%), Gaps = 3/261 (1%) Query: 1 MIDAHIHLEQYEDSELQAMLPGLLEQDIQSLVAVSMNLDSCIRTQEIAARYPGRVKPAYG 60 +ID+HIHL+ Y SE + +L + ++++++VS +L SC ++ R R+KPA+G Sbjct: 9 IIDSHIHLDMYHPSEQKKILADCRKDIVKAMISVSFHLKSCYENLRLS-RMDARIKPAFG 67 Query: 61 FHPEQPAPTESELAALLKWISQHAGEMVAVGEIGLPYYSRAEALARGE--AFEQEPYXXX 118 +HPEQP P++ E+ +L ++++H M AVGE+GLPYY R + R + + PY Sbjct: 68 YHPEQPVPSDEEIQNILSFMNEHKDTMAAVGEVGLPYYMRQKHYMRQKHPELDLAPYLEI 127 Query: 119 XXXXXXXXXXXXKPVVLHAVYEDAITACNLLEKHGIARAHFHWFKGPEEAVTRMIERGYH 178 KPV+LHAVYEDA ++LE+ I +AHFHWFKG +A+ RM+E GY+ Sbjct: 128 LEVFVREAAKLDKPVILHAVYEDADPVISMLERSSIQKAHFHWFKGSSKALQRMVENGYY 187 Query: 179 ISFTPDIVYEPEIRELARRYPPGLVMAETDGPWPFEGPFAGRPTRPAMVHDVAAAWAELH 238 IS TPDI+YE EI +A YP ++ ETDGPW FEG F G+ T P M+H+ AE+ Sbjct: 188 ISVTPDILYEEEIITMAEAYPLERILVETDGPWKFEGMFKGKMTHPEMIHETVNKLAEIK 247 Query: 239 GYSAAQAKAMLTANTARFYQL 259 S ++ NT Y + Sbjct: 248 NESVRDVYKVILNNTRELYSI 268 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.134 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,519,823,189 Number of extensions: 60661364 Number of successful extensions: 147395 Number of sequences better than 1.0e-05: 1426 Number of HSP's gapped: 146441 Number of HSP's successfully gapped: 1426 Length of query: 259 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 126 Effective length of database: 1,807,701,208 Effective search space: 227770352208 Effective search space used: 227770352208 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)