BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0254.0026.p (314 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q3F0D1.1 Q3F0D1_BACTI Transcriptional regulators, LysR family 369 e-100 C3IW60.1 C3IW60_BACTU LysR family transcriptional regulator 369 e-100 B3KJS9.1 B3KJS9_9BACI LysR substrate-binding 358 4e-97 B1HXG1.1 B1HXG1_LYSSC Putative HTH-type transcriptional regulato... 352 3e-95 B1D6B2.1 B1D6B2_9BACL Transcriptional regulator, LysR family 350 1e-94 >Q3F0D1.1 Q3F0D1_BACTI Transcriptional regulators, LysR family Length = 295 Score = 369 bits (947), Expect = e-100, Method: Compositional matrix adjust. Identities = 171/293 (58%), Positives = 223/293 (76%) Query: 17 INIHALRLFYYVAETGSVTKAAARLNISQPAVTSQIKKFEKDLGLPLFNPSGRGVSLTPF 76 +NIH L+LFY VA TGS TKAA + ISQPAV+SQIKKFE ++G+PLF GRGV LT F Sbjct: 1 MNIHGLKLFYQVASTGSFTKAAKLMCISQPAVSSQIKKFEHEIGVPLFKQQGRGVVLTDF 60 Query: 77 GIELAKQAGNFFTYEEQIDEFVEDYRHGRKGKLRIAATYLPANFLVPGWAARFKAGYPEI 136 GI LA++A N T EE I+ F+EDYR + G ++I ATYLPANFL+P WAA FK+ ++ Sbjct: 61 GIVLAEKAKNLVTLEEHIESFIEDYRLAKTGTIQIVATYLPANFLIPKWAATFKSANEDV 120 Query: 137 EMEITTTNSQQAFEQLKRHEADVAFYGGGAQDRPEDIDWLELFEDELWFVVSPSHPYAGR 196 + ITT N++ AFEQL ++AD+A YGGG +RP+++ W ELFEDELWFVVS +HP+A + Sbjct: 121 NLVITTANTKDAFEQLIHYKADIAIYGGGVSERPKEVTWEELFEDELWFVVSTAHPFANK 180 Query: 197 TVSLQEMMLEPFIMREEGSSTRARLVSLCVTYGLKPPQVTLQFSGLGEAIRSVMAGYGAN 256 T+SL EM+ E FIMREEGSS R L SLC T+ ++PP+++LQFSG+ E IRSVMAGYGA+ Sbjct: 181 TISLVEMVKESFIMREEGSSMREHLFSLCQTHQVRPPKISLQFSGINETIRSVMAGYGAS 240 Query: 257 FISSLAVREYVEQKRLSRVQVEGIQLSNHIAICTRSHESVPPALQQFIDICRS 309 FISSLAV EYV+ +L+RV V+ I + + IAICTR E +Q+FI+I ++ Sbjct: 241 FISSLAVSEYVDNGQLARVYVQDIHIKHKIAICTRKAEKHSLLVQKFIEIVKN 293 >C3IW60.1 C3IW60_BACTU LysR family transcriptional regulator Length = 295 Score = 369 bits (947), Expect = e-100, Method: Compositional matrix adjust. Identities = 171/293 (58%), Positives = 223/293 (76%) Query: 17 INIHALRLFYYVAETGSVTKAAARLNISQPAVTSQIKKFEKDLGLPLFNPSGRGVSLTPF 76 +NIH L+LFY VA TGS TKAA + ISQPAV+SQIKKFE ++G+PLF GRGV LT F Sbjct: 1 MNIHGLKLFYQVASTGSFTKAAKLMCISQPAVSSQIKKFEHEIGVPLFKQQGRGVVLTDF 60 Query: 77 GIELAKQAGNFFTYEEQIDEFVEDYRHGRKGKLRIAATYLPANFLVPGWAARFKAGYPEI 136 GI LA++A N T EE I+ F+EDYR + G ++I ATYLPANFL+P WAA FK+ ++ Sbjct: 61 GIVLAEKAKNLVTLEEHIESFIEDYRLAKTGTIQIVATYLPANFLIPKWAATFKSANEDV 120 Query: 137 EMEITTTNSQQAFEQLKRHEADVAFYGGGAQDRPEDIDWLELFEDELWFVVSPSHPYAGR 196 + ITT N++ AFEQL ++AD+A YGGG +RP+++ W ELFEDELWFVVS +HP+A + Sbjct: 121 NLVITTANTKDAFEQLIHYKADIAIYGGGVSERPKEVTWEELFEDELWFVVSTAHPFANK 180 Query: 197 TVSLQEMMLEPFIMREEGSSTRARLVSLCVTYGLKPPQVTLQFSGLGEAIRSVMAGYGAN 256 T+SL EM+ E FIMREEGSS R L SLC T+ ++PP+++LQFSG+ E IRSVMAGYGA+ Sbjct: 181 TISLVEMVKESFIMREEGSSMREHLFSLCQTHQVRPPKISLQFSGINETIRSVMAGYGAS 240 Query: 257 FISSLAVREYVEQKRLSRVQVEGIQLSNHIAICTRSHESVPPALQQFIDICRS 309 FISSLAV EYV+ +L+RV V+ I + + IAICTR E +Q+FI+I ++ Sbjct: 241 FISSLAVSEYVDNGQLARVYVQDIHIKHKIAICTRKAEKHSLLVQKFIEIVKN 293 >B3KJS9.1 B3KJS9_9BACI LysR substrate-binding Length = 295 Score = 358 bits (919), Expect = 4e-97, Method: Compositional matrix adjust. Identities = 168/295 (56%), Positives = 219/295 (74%) Query: 17 INIHALRLFYYVAETGSVTKAAARLNISQPAVTSQIKKFEKDLGLPLFNPSGRGVSLTPF 76 +N+HALRLF+ +A TGSVT+AA LNISQPA+T+Q+KKFEK+L L LF P GRG+ LT Sbjct: 1 MNLHALRLFHVIATTGSVTRAAELLNISQPAITAQVKKFEKELSLTLFKPQGRGIGLTQA 60 Query: 77 GIELAKQAGNFFTYEEQIDEFVEDYRHGRKGKLRIAATYLPANFLVPGWAARFKAGYPEI 136 G EL A F+ E+QI++F DYR G +G +R+AATYLP++FL+P W A++K Y ++ Sbjct: 61 GAELLPLAKRLFSVEQQIEQFCRDYRSGSRGHIRLAATYLPSHFLLPAWLAKYKQRYEDV 120 Query: 137 EMEITTTNSQQAFEQLKRHEADVAFYGGGAQDRPEDIDWLELFEDELWFVVSPSHPYAGR 196 EM ITTTNS A +QL + D+A YGG ++ P+ I ELF DELWFVVSP H YA + Sbjct: 121 EMSITTTNSSDALKQLLNMDVDLAIYGGLPEESPDTIQTEELFRDELWFVVSPDHRYANQ 180 Query: 197 TVSLQEMMLEPFIMREEGSSTRARLVSLCVTYGLKPPQVTLQFSGLGEAIRSVMAGYGAN 256 +SL EMM EPF+MREEGSSTR RL +LC T+ P++TLQF+GL EAI +V+AGYGAN Sbjct: 181 HISLDEMMREPFVMREEGSSTRERLFALCRTHSAPSPRITLQFNGLHEAITAVIAGYGAN 240 Query: 257 FISSLAVREYVEQKRLSRVQVEGIQLSNHIAICTRSHESVPPALQQFIDICRSFP 311 F+SSL VREYVE+ LS+V V+GI+L N IA+CTR HE++ A F+++ R P Sbjct: 241 FVSSLVVREYVERGELSQVYVDGIELQNIIAVCTRKHEALSAAAMNFVEMIRERP 295 >B1HXG1.1 B1HXG1_LYSSC Putative HTH-type transcriptional regulator ycf30 Length = 297 Score = 352 bits (902), Expect = 3e-95, Method: Compositional matrix adjust. Identities = 170/294 (57%), Positives = 217/294 (73%), Gaps = 1/294 (0%) Query: 16 LINIHALRLFYYVAETGSVTKAAARLNISQPAVTSQIKKFEKDLGLPLFNPSGRGVSLTP 75 ++NIH L+LFY+VA TGS TKAA L ISQPAV+SQIK+FE ++G+ LF GRGV LT Sbjct: 1 MMNIHGLKLFYHVATTGSFTKAAELLCISQPAVSSQIKRFEHEIGVQLFKQQGRGVVLTE 60 Query: 76 FGIELAKQAGNFFTYEEQIDEFVEDYRHGRKGKLRIAATYLPANFLVPGWAARFKAGYPE 135 FG LA++A +F E+ I FVEDYR + G L I ATYLPANFL+P WAA FKA Sbjct: 61 FGKALAEKAQHFMALEQHIKSFVEDYRLAKAGDLHIVATYLPANFLIPKWAAAFKAMNEH 120 Query: 136 IEMEITTTNSQQAFEQLKRHEADVAFYGGGAQDRPEDIDWLELFEDELWFVVSPSHPYAG 195 + + ITTTN++ AFEQL ++AD+A YGGG +RP++I W E++EDELWFVVSP H YA Sbjct: 121 VNLMITTTNTKDAFEQLIHYKADIAIYGGGISERPKEIWWEEIYEDELWFVVSPHHRYAH 180 Query: 196 RTVSLQEMMLEPFIMREEGSSTRARLVSLCVTYGLKPPQVTLQFSGLGEAIRSVMAGYGA 255 +T++L EM EPFIMRE+GSS R L SLC TY ++PP++ LQF+G+ E IRSV+AGYGA Sbjct: 181 QTITLIEMFQEPFIMREKGSSMREHLFSLCQTYQVEPPKIALQFNGINETIRSVIAGYGA 240 Query: 256 NFISSLAVREYVEQKRLSRVQVEGIQLSNH-IAICTRSHESVPPALQQFIDICR 308 NFISSLAVREYVE +L+RV ++ I H I+ICTR +E +Q FI+ + Sbjct: 241 NFISSLAVREYVESGQLARVIIKDIHPVKHKISICTRKNEKQNLLVQNFIETIK 294 >B1D6B2.1 B1D6B2_9BACL Transcriptional regulator, LysR family Length = 298 Score = 350 bits (897), Expect = 1e-94, Method: Compositional matrix adjust. Identities = 168/295 (56%), Positives = 215/295 (72%) Query: 17 INIHALRLFYYVAETGSVTKAAARLNISQPAVTSQIKKFEKDLGLPLFNPSGRGVSLTPF 76 +N+HALRLF+ +A GSVT+A+ LNISQPA+T+QIKKFEK+L L LF P GRG++LT Sbjct: 1 MNLHALRLFHTIAAAGSVTRASELLNISQPAITAQIKKFEKELMLTLFKPQGRGIALTDA 60 Query: 77 GIELAKQAGNFFTYEEQIDEFVEDYRHGRKGKLRIAATYLPANFLVPGWAARFKAGYPEI 136 G ELA A F E QI++F +Y G G +R+AATYLPA+FL+P W A+FK + ++ Sbjct: 61 GEELAVLAKRLFAVEHQIEQFAMNYNSGTIGSIRLAATYLPAHFLIPAWIAKFKQQFEQV 120 Query: 137 EMEITTTNSQQAFEQLKRHEADVAFYGGGAQDRPEDIDWLELFEDELWFVVSPSHPYAGR 196 EM ITTTNS A +QL E D+A +GG A++ P+ I ELF DELWFVV+P H YA + Sbjct: 121 EMNITTTNSSGALQQLLDVEVDIAIFGGMAENYPDTIHIEELFHDELWFVVAPGHRYANQ 180 Query: 197 TVSLQEMMLEPFIMREEGSSTRARLVSLCVTYGLKPPQVTLQFSGLGEAIRSVMAGYGAN 256 V+L EMM EPF+MREEGSSTR RL+SLC TY P ++LQF+GL EAI +V+AGYGAN Sbjct: 181 QVTLPEMMKEPFVMREEGSSTRERLLSLCRTYNSAIPTISLQFNGLHEAITAVIAGYGAN 240 Query: 257 FISSLAVREYVEQKRLSRVQVEGIQLSNHIAICTRSHESVPPALQQFIDICRSFP 311 F+SSL VREYVE+ L+RV VEGI L N IAICTR +E +P + + + + R P Sbjct: 241 FVSSLVVREYVERGELARVYVEGIDLKNTIAICTRKNEPLPASAKNLVQMIRQNP 295 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.137 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,870,099,342 Number of extensions: 75400189 Number of successful extensions: 217412 Number of sequences better than 1.0e-05: 15000 Number of HSP's gapped: 212398 Number of HSP's successfully gapped: 15003 Length of query: 314 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 179 Effective length of database: 1,788,859,178 Effective search space: 320205792862 Effective search space used: 320205792862 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)