BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0254.0025.p (223 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q9KBU6.1 Q9KBU6_BACHD O-methyltransferase EC=2.1.1.- 291 3e-77 B1D6B1.1 B1D6B1_9BACL O-methyltransferase family 3 285 2e-75 Q3F0D0.1 Q3F0D0_BACTI O-methyltransferase EC=2.1.1.- 283 9e-75 C3IW59.1 C3IW59_BACTU O-methyltransferase family 3 283 9e-75 A9VQS5.1 A9VQS5_BACWK O-methyltransferase family 3 277 5e-73 >Q9KBU6.1 Q9KBU6_BACHD O-methyltransferase EC=2.1.1.- Length = 223 Score = 291 bits (745), Expect = 3e-77, Method: Compositional matrix adjust. Identities = 137/222 (61%), Positives = 171/222 (77%) Query: 1 MGEMNTWSEVDGYFNSRLLPADPVLDTVLDANAGAGLPAIDVAPNQGKLLYLLAKMKGAA 60 M E TW +D YF+ +L DPV+D VL+AN A LP IDVAPNQGK LYLLAK+KG Sbjct: 1 MEERETWEALDQYFSEKLHAFDPVMDWVLEANEKAELPPIDVAPNQGKFLYLLAKLKGVK 60 Query: 61 NILEIGTLGGYSTIWLARALPEAGRLVSLEYEHKHVVVALDNLRLAGLADKTEIIEGPAL 120 ILEIGTLGGYS+IW+ARALP+ GRLV+LE+ KH +A N++ AG+ K ++I GPAL Sbjct: 61 TILEIGTLGGYSSIWMARALPDDGRLVTLEFSEKHAAIAQSNMKKAGVDGKIDVIIGPAL 120 Query: 121 ESLAALEARGAAPFDFIFIDADKPNNPHYLKWALKLACPGAVIVADNVVRGGEVVQADSK 180 E+L L+ +G + FD IFIDADKPNNPHYL+WALKL+ PG +IV DNVVR G V+ +S Sbjct: 121 ETLPTLKDKGFSSFDMIFIDADKPNNPHYLEWALKLSTPGTLIVGDNVVRNGNVLNKESA 180 Query: 181 DDRVQGIRQFIDLLAAEPRVEATALQTVGSKGYDGFVLGVIS 222 D ++GIRQFI LL+ EPR+E+TA+QTVG KGYDGFV+GV+ Sbjct: 181 DPSIKGIRQFIHLLSEEPRIESTAIQTVGIKGYDGFVVGVVK 222 >B1D6B1.1 B1D6B1_9BACL O-methyltransferase family 3 Length = 224 Score = 285 bits (729), Expect = 2e-75, Method: Compositional matrix adjust. Identities = 136/222 (61%), Positives = 167/222 (75%) Query: 1 MGEMNTWSEVDGYFNSRLLPADPVLDTVLDANAGAGLPAIDVAPNQGKLLYLLAKMKGAA 60 M ++TW +VD Y +L+ D +L+ VL N A LPAIDVAPNQGKLL L A+M GA Sbjct: 2 MSTLHTWEKVDAYLTEQLIQQDTILEQVLANNKQADLPAIDVAPNQGKLLSLYAQMIGAE 61 Query: 61 NILEIGTLGGYSTIWLARALPEAGRLVSLEYEHKHVVVALDNLRLAGLADKTEIIEGPAL 120 ILEIGTLGGYSTIW+ARALPE G+L++LE + H VA N+ LAGL+ K E+ G AL Sbjct: 62 RILEIGTLGGYSTIWMARALPEHGKLITLELDPHHATVAGQNIGLAGLSQKVEVRIGEAL 121 Query: 121 ESLAALEARGAAPFDFIFIDADKPNNPHYLKWALKLACPGAVIVADNVVRGGEVVQADSK 180 E LA L+ GAAPFD IFIDADKPNNPHYL+WAL+ + PG VI+ DNVVR GEV S Sbjct: 122 EQLAKLDEEGAAPFDLIFIDADKPNNPHYLQWALRFSHPGTVIIGDNVVRDGEVTNRHST 181 Query: 181 DDRVQGIRQFIDLLAAEPRVEATALQTVGSKGYDGFVLGVIS 222 D RVQG+R+F++LLA +PR+ ATA+QTVG+KGYDGF LG++ Sbjct: 182 DPRVQGVRKFMELLAEDPRITATAIQTVGAKGYDGFALGIVK 223 >Q3F0D0.1 Q3F0D0_BACTI O-methyltransferase EC=2.1.1.- Length = 220 Score = 283 bits (723), Expect = 9e-75, Method: Compositional matrix adjust. Identities = 133/218 (61%), Positives = 171/218 (78%), Gaps = 1/218 (0%) Query: 5 NTWSEVDGYFNSRLLPADPVLDTVLDANAGAGLPAIDVAPNQGKLLYLLAKMKGAANILE 64 N W +VD YF +L+P+D L+ VL AN AG+P IDV+P QGKLLYLLAK+KGA NILE Sbjct: 4 NVWKDVDEYFIDKLIPSDVTLEGVLQANKEAGIPEIDVSPTQGKLLYLLAKIKGAKNILE 63 Query: 65 IGTLGGYSTIWLARALPEAGRLVSLEYEHKHVVVALDNLRLAGLADKTEIIEGPALESLA 124 IGTLGGYS+IWLARALPE G++ +LE E ++ +A N++ AG K ++I G ALE+L Sbjct: 64 IGTLGGYSSIWLARALPETGKVYTLEIEPEYAKIAKKNIQNAGCTSKVDVIVGKALETLP 123 Query: 125 ALEARGAAPFDFIFIDADKPNNPHYLKWALKLACPGAVIVADNVVRGGEVVQADSKDDRV 184 L+ +G FD IFIDADKPNNPHYLKWAL+LA GA+I++DNVVR GEV+ S DDRV Sbjct: 124 TLKEKGTF-FDLIFIDADKPNNPHYLKWALELANNGALIISDNVVRNGEVIDERSDDDRV 182 Query: 185 QGIRQFIDLLAAEPRVEATALQTVGSKGYDGFVLGVIS 222 QG+R+F+D+L EPR+E+TA+QTVG+KGYDGFV+ ++ Sbjct: 183 QGVRKFMDILEKEPRIESTAIQTVGNKGYDGFVISIVK 220 >C3IW59.1 C3IW59_BACTU O-methyltransferase family 3 Length = 220 Score = 283 bits (723), Expect = 9e-75, Method: Compositional matrix adjust. Identities = 133/218 (61%), Positives = 171/218 (78%), Gaps = 1/218 (0%) Query: 5 NTWSEVDGYFNSRLLPADPVLDTVLDANAGAGLPAIDVAPNQGKLLYLLAKMKGAANILE 64 N W +VD YF +L+P+D L+ VL AN AG+P IDV+P QGKLLYLLAK+KGA NILE Sbjct: 4 NVWKDVDEYFIDKLIPSDVTLEGVLQANKEAGIPEIDVSPTQGKLLYLLAKIKGAKNILE 63 Query: 65 IGTLGGYSTIWLARALPEAGRLVSLEYEHKHVVVALDNLRLAGLADKTEIIEGPALESLA 124 IGTLGGYS+IWLARALPE G++ +LE E ++ +A N++ AG K ++I G ALE+L Sbjct: 64 IGTLGGYSSIWLARALPETGKVYTLEIEPEYAKIAKKNIQNAGCTSKVDVIVGKALETLP 123 Query: 125 ALEARGAAPFDFIFIDADKPNNPHYLKWALKLACPGAVIVADNVVRGGEVVQADSKDDRV 184 L+ +G FD IFIDADKPNNPHYLKWAL+LA GA+I++DNVVR GEV+ S DDRV Sbjct: 124 TLKEKGTF-FDLIFIDADKPNNPHYLKWALELANNGALIISDNVVRNGEVIDERSDDDRV 182 Query: 185 QGIRQFIDLLAAEPRVEATALQTVGSKGYDGFVLGVIS 222 QG+R+F+D+L EPR+E+TA+QTVG+KGYDGFV+ ++ Sbjct: 183 QGVRKFMDILEKEPRIESTAIQTVGNKGYDGFVISIVK 220 >A9VQS5.1 A9VQS5_BACWK O-methyltransferase family 3 Length = 237 Score = 277 bits (708), Expect = 5e-73, Method: Compositional matrix adjust. Identities = 136/222 (61%), Positives = 160/222 (72%) Query: 1 MGEMNTWSEVDGYFNSRLLPADPVLDTVLDANAGAGLPAIDVAPNQGKLLYLLAKMKGAA 60 M + W+ VD Y + L+P D L+ VL ANA A LPA DV+P QGK L LL +++GA Sbjct: 15 MSTIEKWTAVDQYMSDVLIPKDSTLEEVLQANASANLPAHDVSPTQGKFLQLLVQIQGAR 74 Query: 61 NILEIGTLGGYSTIWLARALPEAGRLVSLEYEHKHVVVALDNLRLAGLADKTEIIEGPAL 120 NILEIGTLGGYSTIWLARALP GR+V+LE KH +A N+ A L DK EI G AL Sbjct: 75 NILEIGTLGGYSTIWLARALPSGGRIVTLEASEKHAEIARSNIERANLNDKIEIRLGLAL 134 Query: 121 ESLAALEARGAAPFDFIFIDADKPNNPHYLKWALKLACPGAVIVADNVVRGGEVVQADSK 180 +SL +E PFDFIFIDADK NNP Y +WALKL+ PG VI+ DNVVR GEV+ S Sbjct: 135 DSLKQIENEKYEPFDFIFIDADKQNNPAYFEWALKLSRPGTVIIGDNVVREGEVIDNTSS 194 Query: 181 DDRVQGIRQFIDLLAAEPRVEATALQTVGSKGYDGFVLGVIS 222 D RVQGIR F +L+AAEPRV ATALQTVGSKGYDGFV+ V++ Sbjct: 195 DPRVQGIRHFYELIAAEPRVSATALQTVGSKGYDGFVMAVVT 236 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.138 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,364,457,740 Number of extensions: 54868186 Number of successful extensions: 138511 Number of sequences better than 1.0e-05: 1309 Number of HSP's gapped: 137384 Number of HSP's successfully gapped: 1309 Length of query: 223 Length of database: 3,060,696,203 Length adjustment: 131 Effective length of query: 92 Effective length of database: 1,826,543,238 Effective search space: 168041977896 Effective search space used: 168041977896 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)