BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0254.0021.p (207 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q81BJ4.1 Q81BJ4_BACCR Transcriptional regulator, TetR family 284 3e-75 C2T2Y2.1 C2T2Y2_BACCE Transcriptional regulator, TetR 283 8e-75 C2V5B3.1 C2V5B3_BACCE Transcriptional regulator, TetR 283 9e-75 C2U7Q5.1 C2U7Q5_BACCE Transcriptional regulator, TetR 282 1e-74 C2XKJ8.1 C2XKJ8_BACCE Transcriptional regulator, TetR 281 3e-74 >Q81BJ4.1 Q81BJ4_BACCR Transcriptional regulator, TetR family Length = 192 Score = 284 bits (727), Expect = 3e-75, Method: Compositional matrix adjust. Identities = 134/190 (70%), Positives = 160/190 (84%) Query: 17 MDKKRGRPRNNEAEAAILAASYDLLLETGFGAVTVEKIAERAKVSKATIYKWWPNKAAVV 76 M KRGRPRN E + AIL+ASY+LLLE+GF AVTV+KIAERAKVSKATIYKWWPNKAAVV Sbjct: 1 MQSKRGRPRNIETQKAILSASYELLLESGFKAVTVDKIAERAKVSKATIYKWWPNKAAVV 60 Query: 77 IDGYLSAATARLPVPDTGSVKEDIRIHATNLAAFLYSREGKVITEMIGEGQFDAGLAEAY 136 +DG+LSAA ARLPVPDTGS DI IHAT+LA FL SREG +I E++GEGQFD+ LAE Y Sbjct: 61 MDGFLSAAAARLPVPDTGSALNDILIHATSLANFLISREGTIINELVGEGQFDSKLAEEY 120 Query: 137 RTRFFAPRRQEARQLLERGVMRGELKKDLDVNCSIDLMYGPIFYRLLITGDTLNGEFVEK 196 R R+F PRR +A+QLLE+G+ RGELK++LD+ SIDL+YGPIFYRLL+TG+ L+ +V Sbjct: 121 RVRYFQPRRLQAKQLLEKGIKRGELKENLDIELSIDLIYGPIFYRLLVTGEKLDDSYVHD 180 Query: 197 LLALAFEGIR 206 L+ AFEGIR Sbjct: 181 LVINAFEGIR 190 >C2T2Y2.1 C2T2Y2_BACCE Transcriptional regulator, TetR Length = 195 Score = 283 bits (723), Expect = 8e-75, Method: Compositional matrix adjust. Identities = 134/193 (69%), Positives = 162/193 (83%) Query: 14 VIIMDKKRGRPRNNEAEAAILAASYDLLLETGFGAVTVEKIAERAKVSKATIYKWWPNKA 73 +I + KRGRPRN E + AIL+ASY+LLLE+GF AVTV+KIAERAKVSKATIYKWWPNKA Sbjct: 1 MINVQSKRGRPRNIETQKAILSASYELLLESGFKAVTVDKIAERAKVSKATIYKWWPNKA 60 Query: 74 AVVIDGYLSAATARLPVPDTGSVKEDIRIHATNLAAFLYSREGKVITEMIGEGQFDAGLA 133 AVV+DG+LSAA ARLPVPDTGS DI IHAT+LA FL SREG +I E++GEGQFD+ LA Sbjct: 61 AVVMDGFLSAAAARLPVPDTGSALNDILIHATSLANFLISREGTIINELVGEGQFDSKLA 120 Query: 134 EAYRTRFFAPRRQEARQLLERGVMRGELKKDLDVNCSIDLMYGPIFYRLLITGDTLNGEF 193 E YR R+F PRR +A+QLLE+G+ RGELK++LD+ SIDL+YGPIFYRLL+TG+ L+ + Sbjct: 121 EEYRVRYFQPRRLQAKQLLEKGIKRGELKENLDIELSIDLIYGPIFYRLLVTGEKLDDSY 180 Query: 194 VEKLLALAFEGIR 206 V L+ AFEGIR Sbjct: 181 VHDLVINAFEGIR 193 >C2V5B3.1 C2V5B3_BACCE Transcriptional regulator, TetR Length = 200 Score = 283 bits (723), Expect = 9e-75, Method: Compositional matrix adjust. Identities = 135/198 (68%), Positives = 166/198 (83%) Query: 9 IFKQEVIIMDKKRGRPRNNEAEAAILAASYDLLLETGFGAVTVEKIAERAKVSKATIYKW 68 +F + +I + KRGRPRN E + AIL+ASY+LLLE+GF AVTV+KIA+RAKVSKATIYKW Sbjct: 1 MFLKGMINVQGKRGRPRNIETQKAILSASYELLLESGFKAVTVDKIADRAKVSKATIYKW 60 Query: 69 WPNKAAVVIDGYLSAATARLPVPDTGSVKEDIRIHATNLAAFLYSREGKVITEMIGEGQF 128 WPNKAAVV+DG+LSAA ARLPVPDTGS DI HAT+LA FL SREG +ITE++GEGQF Sbjct: 61 WPNKAAVVMDGFLSAAAARLPVPDTGSALTDILTHATSLANFLISREGTIITELVGEGQF 120 Query: 129 DAGLAEAYRTRFFAPRRQEARQLLERGVMRGELKKDLDVNCSIDLMYGPIFYRLLITGDT 188 D+ LAE YR R+F PRR +A+QLLE+G+ RGELK++LDV+ SIDL+YGPIFYRLL+TG+ Sbjct: 121 DSKLAEEYRARYFQPRRLQAKQLLEKGIKRGELKENLDVDLSIDLIYGPIFYRLLVTGEK 180 Query: 189 LNGEFVEKLLALAFEGIR 206 L+ +V L+ AFEGIR Sbjct: 181 LDDSYVHDLVINAFEGIR 198 >C2U7Q5.1 C2U7Q5_BACCE Transcriptional regulator, TetR Length = 200 Score = 282 bits (722), Expect = 1e-74, Method: Compositional matrix adjust. Identities = 134/198 (67%), Positives = 167/198 (84%) Query: 9 IFKQEVIIMDKKRGRPRNNEAEAAILAASYDLLLETGFGAVTVEKIAERAKVSKATIYKW 68 +F + +I + KRGRPRN E + AIL+ASY+LLLE+GF AVTV+KIA+RAKVSKATIYKW Sbjct: 1 MFLKGMINVQGKRGRPRNIETQKAILSASYELLLESGFKAVTVDKIADRAKVSKATIYKW 60 Query: 69 WPNKAAVVIDGYLSAATARLPVPDTGSVKEDIRIHATNLAAFLYSREGKVITEMIGEGQF 128 WPNKAAVV+DG+LSAA ARLPVPDTGS DI HAT+LA+FL SREG +ITE++GEGQF Sbjct: 61 WPNKAAVVMDGFLSAAAARLPVPDTGSALTDILTHATSLASFLISREGTIITELVGEGQF 120 Query: 129 DAGLAEAYRTRFFAPRRQEARQLLERGVMRGELKKDLDVNCSIDLMYGPIFYRLLITGDT 188 D+ LAE YR R+F PRR +A+QLLE+G+ RGELK++LDV+ SIDL+YGPIFYRLL+TG+ Sbjct: 121 DSKLAEEYRARYFQPRRLQAKQLLEKGIKRGELKENLDVDLSIDLIYGPIFYRLLVTGEK 180 Query: 189 LNGEFVEKLLALAFEGIR 206 L+ +V L+ AFEGI+ Sbjct: 181 LDESYVHDLVINAFEGIQ 198 >C2XKJ8.1 C2XKJ8_BACCE Transcriptional regulator, TetR Length = 195 Score = 281 bits (719), Expect = 3e-74, Method: Compositional matrix adjust. Identities = 133/193 (68%), Positives = 163/193 (84%) Query: 14 VIIMDKKRGRPRNNEAEAAILAASYDLLLETGFGAVTVEKIAERAKVSKATIYKWWPNKA 73 +I + KRGRPRN E + AIL+ASY+LLLE+GF AVTV+KIA+RAKVSKATIYKWWPNKA Sbjct: 1 MINVQGKRGRPRNVETQKAILSASYELLLESGFKAVTVDKIADRAKVSKATIYKWWPNKA 60 Query: 74 AVVIDGYLSAATARLPVPDTGSVKEDIRIHATNLAAFLYSREGKVITEMIGEGQFDAGLA 133 AVV+DG+L AA ARLP+PDTGS DI IHAT+L FL SREG +ITE++GEGQFD+ LA Sbjct: 61 AVVMDGFLFAAAARLPIPDTGSALNDILIHATSLTKFLISREGTIITELVGEGQFDSKLA 120 Query: 134 EAYRTRFFAPRRQEARQLLERGVMRGELKKDLDVNCSIDLMYGPIFYRLLITGDTLNGEF 193 E YRTR+F PRR +A+QLLE+G+ RGELK++LDV+ SIDL+YGPIFYRLL+TG+ L+ + Sbjct: 121 EEYRTRYFQPRRLQAKQLLEKGIKRGELKENLDVDLSIDLIYGPIFYRLLVTGEKLDDSY 180 Query: 194 VEKLLALAFEGIR 206 V L+ AFEGIR Sbjct: 181 VHDLVINAFEGIR 193 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.137 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,139,932,690 Number of extensions: 44395009 Number of successful extensions: 131321 Number of sequences better than 1.0e-05: 475 Number of HSP's gapped: 131133 Number of HSP's successfully gapped: 475 Length of query: 207 Length of database: 3,060,696,203 Length adjustment: 129 Effective length of query: 78 Effective length of database: 1,845,385,268 Effective search space: 143940050904 Effective search space used: 143940050904 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 128 (53.9 bits)