BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0254.0018.p (87 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value O65998.1 O65998_CLOAB Orf36 60 4e-08 Q97IY0.1 Q97IY0_CLOAB Putative uncharacterized protein 60 5e-08 >O65998.1 O65998_CLOAB Orf36 Length = 316 Score = 60.5 bits (145), Expect = 4e-08, Method: Compositional matrix adjust. Identities = 31/86 (36%), Positives = 51/86 (59%) Query: 2 NGKYLDLIFTSEKIGQKLSMQFRYSDEETAYEMSTSGKMEKVQVNGADAVMMDDRSLHWE 61 N KY+ + F + K + +Q R S EET Y M ++K+++N DAV+ + S+ WE Sbjct: 231 NNKYITMYFKNPKNPNVMFLQERLSCEETKYTMGGEDDIKKIKINNVDAVLSGNESIDWE 290 Query: 62 ADGVLYAMNTCGLERSEVLKIAESIR 87 + LY++ G+ +E+ KIAESI+ Sbjct: 291 YNKSLYSLILKGISVNELEKIAESIK 316 >Q97IY0.1 Q97IY0_CLOAB Putative uncharacterized protein Length = 316 Score = 60.5 bits (145), Expect = 5e-08, Method: Compositional matrix adjust. Identities = 31/86 (36%), Positives = 51/86 (59%) Query: 2 NGKYLDLIFTSEKIGQKLSMQFRYSDEETAYEMSTSGKMEKVQVNGADAVMMDDRSLHWE 61 N KY+ + F + K + +Q R S EET Y M ++K+++N DAV+ + S+ WE Sbjct: 231 NNKYITMYFKNPKNPNVMFLQERLSCEETKYTMGGEDDIKKIKINNVDAVLSGNESIDWE 290 Query: 62 ADGVLYAMNTCGLERSEVLKIAESIR 87 + LY++ G+ +E+ KIAESI+ Sbjct: 291 YNKSLYSLILKGISVNELEKIAESIK 316 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.314 0.129 0.356 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 444,323,753 Number of extensions: 13836607 Number of successful extensions: 29283 Number of sequences better than 1.0e-05: 2 Number of HSP's gapped: 29283 Number of HSP's successfully gapped: 2 Length of query: 87 Length of database: 3,060,696,203 Length adjustment: 57 Effective length of query: 30 Effective length of database: 2,523,698,348 Effective search space: 75710950440 Effective search space used: 75710950440 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 125 (52.8 bits)