BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0254.0013.p (400 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KE12.1 B3KE12_9BACI Alanine racemase EC=5.1.1.1 580 e-163 B1DH88.1 B1DH88_9BACL Alanine racemase EC=5.1.1.1 449 e-124 B2A571.1 B2A571_NATTJ Alanine racemase EC=5.1.1.1 357 1e-96 A3DIW7.1 A3DIW7_CLOTH Alanine racemase EC=5.1.1.1 340 1e-91 B4BHP3.1 B4BHP3_CLOTM Alanine racemase EC=5.1.1.1 340 1e-91 >B3KE12.1 B3KE12_9BACI Alanine racemase EC=5.1.1.1 Length = 395 Score = 580 bits (1494), Expect = e-163, Method: Compositional matrix adjust. Identities = 283/391 (72%), Positives = 321/391 (82%), Gaps = 1/391 (0%) Query: 8 VQASYRPTVAEINLDDLRANYEAFRKALPAETKFMGCVKGNAYGHGAVEVTRELERLGAD 67 +QA YRPT+AEINLD LRANY AFR+ALP K + CVK NAYGHGAVEV REL++LGA Sbjct: 1 MQAIYRPTLAEINLDHLRANYNAFREALPDHMKLLACVKANAYGHGAVEVARELQQLGAA 60 Query: 68 YVSVAFLDEALELRQAGILIPILVLGYTSPEGIPVAWKNHVTVTLFTPEVLEAVRQLPVD 127 Y+SVAFLDEA+ELRQ GI +PILVLGYT PE + +A++ VT+TLF+ EVL+A++ LPV Sbjct: 61 YLSVAFLDEAIELRQGGISVPILVLGYTPPEAVGIAYEYDVTITLFSSEVLQAIKALPVK 120 Query: 128 PEHRLKVHIKIDSGMGRLGLLPA-DAPDFIAEVHSVAQAELEGMFTHFAKADESNKSYTL 186 PE LKVHIKIDSGMGRLGLLP DA FI E S+ Q +EGMFTHFA+ADE NK YTL Sbjct: 121 PEKPLKVHIKIDSGMGRLGLLPGEDAISFIEEAFSLPQVVVEGMFTHFARADEENKDYTL 180 Query: 187 MQYRRFMSVAETLRDRDIHIPIIHTGNSATAIDTPLLSSNMVRVGISLYGFYPSTEVNRE 246 QYRRF VA+ LRD+D HIPIIHTGNSA AIDTP LS NMVRVGI LYG YPS EVNR+ Sbjct: 181 GQYRRFQGVADALRDKDYHIPIIHTGNSAAAIDTPELSYNMVRVGIGLYGLYPSDEVNRK 240 Query: 247 LVALHPVMTLKTQAVYVKTLPPDWGISYGTRYFTDSEEIIATLPVGYADGYSRMLTGKAE 306 V+L PVMTLKT+ VYVK LPP GISYGT+Y T+ EE I TLP+GYADG+SRML+GKAE Sbjct: 241 TVSLRPVMTLKTKLVYVKNLPPHSGISYGTKYVTEQEERIGTLPIGYADGFSRMLSGKAE 300 Query: 307 VLIRGRRVPVVGTICMDQCMVTLKSFAEEAEQIKAGEEVVLIGRQGAVSITADELALHLG 366 VLIRGRRVPVVG ICMDQCMV+LKSFAEEAEQIKAGEEVVLIG+Q ITADELA LG Sbjct: 301 VLIRGRRVPVVGNICMDQCMVSLKSFAEEAEQIKAGEEVVLIGQQSGECITADELAAKLG 360 Query: 367 TIHYEVICMLAHRVPRVYVREGAAPNLVNPL 397 TIHYEV+CM+AHRVPR+Y+R+ VN L Sbjct: 361 TIHYEVVCMIAHRVPRLYIRDDIPVKTVNAL 391 >B1DH88.1 B1DH88_9BACL Alanine racemase EC=5.1.1.1 Length = 394 Score = 449 bits (1155), Expect = e-124, Method: Compositional matrix adjust. Identities = 227/393 (57%), Positives = 281/393 (71%), Gaps = 3/393 (0%) Query: 8 VQASYRPTVAEINLDDLRANYEAFRKALPAETKFMGCVKGNAYGHGAVEVTRELERLGAD 67 +++ YRPT EI+LD L N AFR A+P M VK NAYGHGAVE+ RE ER G D Sbjct: 1 MESYYRPTRVEISLDALNRNLHAFRGAMPEGRLLMASVKANAYGHGAVEIAREAERFGVD 60 Query: 68 YVSVAFLDEALELRQAGILIPILVLGYTSPEGIPVAWKNHVTVTLFTPEVLEAVRQLPVD 127 Y+ VAFLDEAL+LR AGI PILVLG+ + + +A +T+ LF ++LEA LP Sbjct: 61 YLGVAFLDEALQLRHAGIATPILVLGFVPADALAIARDKDITIALFREDILEAAAALPAQ 120 Query: 128 PE-HRLKVHIKIDSGMGRLGLLPAD-APDFIAEVHSVAQAELEGMFTHFAKADESNKSYT 185 + +LK HIKIDSGMGRLG++ D A FI + V Q E+EGMFTH+AKADE++KSYT Sbjct: 121 ADGKKLKAHIKIDSGMGRLGIIGKDEAIAFIRKALDVPQLEIEGMFTHYAKADETDKSYT 180 Query: 186 LMQYRRFMSVAETLRDRDIHIPIIHTGNSATAIDTPLLSSNMVRVGISLYGFYPSTEVNR 245 QY RF +VAE +R + I I+H NSA IDTP +MVR+GIS+YG YPS EV+R Sbjct: 181 RQQYERFHAVAEYVRSNKLPIRILHAANSAAGIDTPEYGGDMVRLGISMYGLYPSDEVSR 240 Query: 246 ELVALHPVMTLKTQAVYVKTLPPDWGISYGTRYFTDSEEIIATLPVGYADGYSRMLTGKA 305 E + L PV++LK++ V VK PP WGISYGTRY T EE I TLP+GYADG+SRML+GKA Sbjct: 241 ERIKLEPVLSLKSEVVMVKKTPPGWGISYGTRYVTKQEECIGTLPIGYADGFSRMLSGKA 300 Query: 306 EVLIRGRRVPVVGTICMDQCMVTLKSFAEEAEQIKAGEEVVLIGRQGAVSITADELALHL 365 VL+RG +VPV GTICMDQCM+ L + +A ++ GEEVVLIGRQG I+A+ELAL L Sbjct: 301 HVLVRGVKVPVRGTICMDQCMIALDPASADA-AVEPGEEVVLIGRQGGEEISAEELALQL 359 Query: 366 GTIHYEVICMLAHRVPRVYVREGAAPNLVNPLL 398 GTI+YEV CMLA RVPRVYVR+G + NPL+ Sbjct: 360 GTINYEVTCMLAARVPRVYVRDGQVAAVSNPLV 392 >B2A571.1 B2A571_NATTJ Alanine racemase EC=5.1.1.1 Length = 390 Score = 357 bits (915), Expect = 1e-96, Method: Compositional matrix adjust. Identities = 174/376 (46%), Positives = 246/376 (65%), Gaps = 6/376 (1%) Query: 13 RPTVAEINLDDLRANYEAFRKALPAETKFMGCVKGNAYGHGAVEVTRELERLGADYVSVA 72 RPT +EINLD+L N+ R+ + K MG +K + YGHGAVE+ EL G DY+ VA Sbjct: 8 RPTFSEINLDNLSYNFNRIREHV-NNKKIMGIIKADGYGHGAVEIAEELLNQGVDYLGVA 66 Query: 73 FLDEALELRQAGILIPILVLGYTSPEGIPVAWKNHVTVTLFTPEVLEAVRQLPVDPEHRL 132 LDEA+ELR+AGI PIL+LGYT E + V K +T T++T E+ EA+ Q Sbjct: 67 ILDEAVELRKAGIAAPILILGYTPVEHVDVCLKYGITPTIYTYEMAEALSQTARKMGKTA 126 Query: 133 KVHIKIDSGMGRLGLLPADAPDFIAEVHSVAQAELEGMFTHFAKADESNKSYTLMQYRRF 192 VH+KID+GMGR+G+ P ++ +F+ + + E+EG+FTHF+ ADE +K+YT Q F Sbjct: 127 FVHVKIDTGMGRIGIAPENSAEFVKNISKLPSLEVEGVFTHFSVADEEDKTYTQQQINSF 186 Query: 193 MSVAETLRDRDIHIPIIHTGNSATAIDTPLLSSNMVRVGISLYGFYPSTEVNRELVALHP 252 + L D I IP+ H NS ID P +MVRVG++L+G YPS + +E ++L P Sbjct: 187 EKALKMLEDEGIEIPLKHAANSPATIDLPDAYFDMVRVGLALFGLYPSPHM-KEKISLKP 245 Query: 253 VMTLKTQAVYVKTLPPDWGISYGTRYFTDSEEIIATLPVGYADGYSRMLTGKAEVLIRGR 312 VM+LKTQ +VK +P ISYG ++ T+ E II TLPVGYADG++R+L+ EV++ G+ Sbjct: 246 VMSLKTQVSHVKQVPKGTSISYGRKFITEDESIIGTLPVGYADGFTRLLSNIGEVIVNGK 305 Query: 313 RVPVVGTICMDQCMVTLKSFAEEAEQIKAGEEVVLIGRQGAVSITADELALHLGTIHYEV 372 RVP+VG ICMDQCM ++ +I G+EVV+IG Q IT +++A LGTI+YE+ Sbjct: 306 RVPIVGAICMDQCMF----LGDDVPEIGIGDEVVIIGEQDGTQITVEDIADKLGTINYEI 361 Query: 373 ICMLAHRVPRVYVREG 388 + M+ RVPR+Y++ G Sbjct: 362 VTMVDKRVPRLYLKNG 377 >A3DIW7.1 A3DIW7_CLOTH Alanine racemase EC=5.1.1.1 Length = 391 Score = 340 bits (873), Expect = 1e-91, Method: Compositional matrix adjust. Identities = 168/383 (43%), Positives = 247/383 (64%), Gaps = 4/383 (1%) Query: 17 AEINLDDLRANYEAFRKALPAETKFMGCVKGNAYGHGAVEVTRELERLGADYVSVAFLDE 76 AE+NLD++ N + R+ + + + MG VK +AYGHG +E+ R L G ++V+ LDE Sbjct: 12 AEVNLDNIAHNVKEIRRIVDKKVEIMGVVKADAYGHGVMEIARTLLENGVTRLAVSMLDE 71 Query: 77 ALELRQAGILIPILVLGYTSPEGIPVAWKNHVTVTLFTPEVLEAVRQLPVDPEHRLKVHI 136 A++LRQ GI +PIL+L YT P N VT T+F+ ++ EA+ + V +K+HI Sbjct: 72 AIQLRQNGIKVPILILSYTDPVRAEEIVLNDVTQTVFSHDLAEALSEAAVKHHRNVKIHI 131 Query: 137 KIDSGMGRLGLLPA-DAPDFIAEVHSVAQAELEGMFTHFAKADESNKSYTLMQYRRFMSV 195 KID+GM R+G +P A + ++ + +EG+FTHFA ADE++KSYT MQ+ RFMS+ Sbjct: 132 KIDTGMTRVGFMPGYSAVKNVVQISKLPGIIIEGLFTHFASADEADKSYTYMQFERFMSI 191 Query: 196 AETLRDRDIHIPIIHTGNSATAIDTPLLSSNMVRVGISLYGFYPSTEVNRELVALHPVMT 255 L ++IP+ H NSA I+ P + NMVR GI+LYG YPS EV++ + L P M+ Sbjct: 192 VGELNRIGVYIPVKHVCNSAALIEFPEMHLNMVRPGIALYGLYPSDEVDKTKIDLRPAMS 251 Query: 256 LKTQAVYVKTLPPDWGISYGTRYFTDSEEIIATLPVGYADGYSRMLTGKAEVLIRGRRVP 315 LK + VK + D ISYG + T IAT+P+GYADGY+R+LTGK +VL+ G+ P Sbjct: 252 LKANVILVKDVEKDTFISYGRIFKTSRNSRIATIPIGYADGYTRLLTGKGKVLLNGQLAP 311 Query: 316 VVGTICMDQCMVTLKSFAEEAEQIKAGEEVVLIGRQGAVSITADELALHLGTIHYEVICM 375 +VG ICMDQCMV + + +K G+E VLIG+Q I ++LA +GTI+YE++ + Sbjct: 312 IVGRICMDQCMVDITDIEGD---VKVGDEAVLIGKQKDNEIKVEDLAKSVGTINYELVSI 368 Query: 376 LAHRVPRVYVREGAAPNLVNPLL 398 + R+PRVY++EG N++N L+ Sbjct: 369 IGKRIPRVYLKEGKIYNVLNYLI 391 >B4BHP3.1 B4BHP3_CLOTM Alanine racemase EC=5.1.1.1 Length = 391 Score = 340 bits (873), Expect = 1e-91, Method: Compositional matrix adjust. Identities = 168/383 (43%), Positives = 247/383 (64%), Gaps = 4/383 (1%) Query: 17 AEINLDDLRANYEAFRKALPAETKFMGCVKGNAYGHGAVEVTRELERLGADYVSVAFLDE 76 AE+NLD++ N + R+ + + + MG VK +AYGHG +E+ R L G ++V+ LDE Sbjct: 12 AEVNLDNIAHNVKEIRRIVDKKVEIMGVVKADAYGHGVMEIARTLLENGVTRLAVSMLDE 71 Query: 77 ALELRQAGILIPILVLGYTSPEGIPVAWKNHVTVTLFTPEVLEAVRQLPVDPEHRLKVHI 136 A++LRQ GI +PIL+L YT P N VT T+F+ ++ EA+ + V +K+HI Sbjct: 72 AIQLRQNGIKVPILILSYTDPVRAEEIVLNDVTQTVFSHDLAEALSEAAVKHHRNVKIHI 131 Query: 137 KIDSGMGRLGLLPA-DAPDFIAEVHSVAQAELEGMFTHFAKADESNKSYTLMQYRRFMSV 195 KID+GM R+G +P A + ++ + +EG+FTHFA ADE++KSYT MQ+ RFMS+ Sbjct: 132 KIDTGMTRVGFMPGYSAVKNVVQISKLPGIIIEGLFTHFASADEADKSYTYMQFERFMSI 191 Query: 196 AETLRDRDIHIPIIHTGNSATAIDTPLLSSNMVRVGISLYGFYPSTEVNRELVALHPVMT 255 L ++IP+ H NSA I+ P + NMVR GI+LYG YPS EV++ + L P M+ Sbjct: 192 VGELNRIGVYIPVKHVCNSAALIEFPEMHLNMVRPGIALYGLYPSDEVDKTKIDLRPAMS 251 Query: 256 LKTQAVYVKTLPPDWGISYGTRYFTDSEEIIATLPVGYADGYSRMLTGKAEVLIRGRRVP 315 LK + VK + D ISYG + T IAT+P+GYADGY+R+LTGK +VL+ G+ P Sbjct: 252 LKANVILVKDVEKDTFISYGRIFKTSRNSRIATIPIGYADGYTRLLTGKGKVLLNGQLAP 311 Query: 316 VVGTICMDQCMVTLKSFAEEAEQIKAGEEVVLIGRQGAVSITADELALHLGTIHYEVICM 375 +VG ICMDQCMV + + +K G+E VLIG+Q I ++LA +GTI+YE++ + Sbjct: 312 IVGRICMDQCMVDITDIEGD---VKVGDEAVLIGKQKDNEIKVEDLAKSVGTINYELVSI 368 Query: 376 LAHRVPRVYVREGAAPNLVNPLL 398 + R+PRVY++EG N++N L+ Sbjct: 369 IGKRIPRVYLKEGKIYNVLNYLI 391 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.137 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,374,473,734 Number of extensions: 99411211 Number of successful extensions: 255976 Number of sequences better than 1.0e-05: 1928 Number of HSP's gapped: 248349 Number of HSP's successfully gapped: 1935 Length of query: 400 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 262 Effective length of database: 1,760,596,133 Effective search space: 461276186846 Effective search space used: 461276186846 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)