BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0254.0007.p (272 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q2B7V7.1 Q2B7V7_9BACI Putative uncharacterized protein 285 2e-75 O32153.1 YURL_BACSU Uncharacterized sugar kinase yurL EC=2.7.1.- 240 8e-62 A7Z8G8.1 A7Z8G8_BACA2 YurL 223 2e-56 C1D442.1 C1D442_DEIDV Putative fructoselysine kinase 183 1e-44 Q44323.1 Q44323_9RHIZ MocE 171 6e-41 >Q2B7V7.1 Q2B7V7_9BACI Putative uncharacterized protein Length = 273 Score = 285 bits (730), Expect = 2e-75, Method: Compositional matrix adjust. Identities = 136/268 (50%), Positives = 178/268 (66%), Gaps = 1/268 (0%) Query: 1 MRMIAVGDNVADCYLDQGLYYPGGNAVNVAVNCKRNGCEEVSYIGVFGNDKKSDHIMDSL 60 M+++AVGDNV DCYLD+ Y+PGGN VNVAVN KRNG V YIG+F D ++HI +L Sbjct: 3 MKLMAVGDNVVDCYLDRNEYFPGGNCVNVAVNAKRNGASRVGYIGIFATDGPAEHIKTAL 62 Query: 61 DKEGINYRYSRRVYADSGSPGVKLV-GNDRVFVRGAQDTAQHLLRLRLMPADLEYIAGHD 119 KEG+++++SR +G P V L NDRVFV G +DT QH +++L+P +++YI D Sbjct: 63 QKEGVDFQFSRDAVGITGQPKVALTEDNDRVFVGGPKDTVQHRFKIQLIPEEIDYIKQFD 122 Query: 120 VCHTSCYSSIERELPVIKEHCDISFDFSTRTDEDYLGQVCPHIKYAFFSGAELDSVQIEA 179 VCH SCYSS+E EL I + +S+DFS R D +Y+ + P I YAFFS AEL ++ Sbjct: 123 VCHVSCYSSMESELAKIAKEIKVSYDFSNRKDLEYISSIAPSITYAFFSAAELSEAELHG 182 Query: 180 LIKTCHRLGTEIVGITKGSEGALFSKNGVLYKQGVTPAEVVDTMGAGDSFIAGFLVSYLK 239 I G EI+G+T+G E A+F +YKQ + VVDTMGAGDS IAGFL Y+K Sbjct: 183 FIAELAPFGFEIIGLTRGGEPAVFVHQENIYKQKLRKINVVDTMGAGDSLIAGFLTEYVK 242 Query: 240 KGVMEEALQSGANSAAYTCTFYGGFGYP 267 G +E+AL+ G SA TC YGGFGYP Sbjct: 243 SGDVEKALEKGTLSAEKTCQEYGGFGYP 270 >O32153.1 YURL_BACSU Uncharacterized sugar kinase yurL EC=2.7.1.- Length = 284 Score = 240 bits (613), Expect = 8e-62, Method: Compositional matrix adjust. Identities = 124/270 (45%), Positives = 174/270 (64%), Gaps = 2/270 (0%) Query: 1 MRMIAVGDNVADCYLDQGLYYPGGNAVNVAVNCKRNGCEEVSYIGVFGNDKKSDHIMDSL 60 M++IAVGDNV D Y DQ +YPGGNA+NVAV KR G E SYIG+ GND+ + H+++ L Sbjct: 1 MKLIAVGDNVVDYYQDQETFYPGGNALNVAVLAKRLG-HESSYIGIVGNDEAAAHLLNVL 59 Query: 61 DKEGINYRYSRRVYADSGSPGVKL-VGNDRVFVRGAQDTAQHLLRLRLMPADLEYIAGHD 119 E +N Y R+ + ++G V L DR+FVR + Q LRL D+ +I+GHD Sbjct: 60 KLEQVNADYIRQAHGENGMAIVTLDEQGDRIFVRSNKGGIQSRLRLAFQEKDVSFISGHD 119 Query: 120 VCHTSCYSSIERELPVIKEHCDISFDFSTRTDEDYLGQVCPHIKYAFFSGAELDSVQIEA 179 + HTS YS +E +LP + +SFDFST ++DYL +VCP++ YAFFSG++L + Sbjct: 120 LLHTSVYSRLENDLPQLCGLVPVSFDFSTNREDDYLRRVCPYVTYAFFSGSDLSESECGE 179 Query: 180 LIKTCHRLGTEIVGITKGSEGALFSKNGVLYKQGVTPAEVVDTMGAGDSFIAGFLVSYLK 239 L KT H G ++V +T+G +GA+ S +Y Q + A+++DT+GAGDSFIAGFL ++ Sbjct: 180 LAKTAHGYGAKMVCMTRGGQGAILSAGDRVYHQPIVEADIIDTLGAGDSFIAGFLTAFCV 239 Query: 240 KGVMEEALQSGANSAAYTCTFYGGFGYPKP 269 K + AL+ A +AA TC YG FGY P Sbjct: 240 KQDITYALRQAAETAAKTCGVYGAFGYGYP 269 >A7Z8G8.1 A7Z8G8_BACA2 YurL Length = 284 Score = 223 bits (567), Expect = 2e-56, Method: Compositional matrix adjust. Identities = 122/270 (45%), Positives = 167/270 (61%), Gaps = 2/270 (0%) Query: 1 MRMIAVGDNVADCYLDQGLYYPGGNAVNVAVNCKRNGCEEVSYIGVFGNDKKSDHIMDSL 60 M++IA GDNV D Y DQ +YPGGNA+NVAV +R G E SYIG+ G+D+ +DH+++ L Sbjct: 1 MKLIAAGDNVVDYYQDQETFYPGGNALNVAVLARRLG-HETSYIGILGSDEAADHVLNVL 59 Query: 61 DKEGINYRYSRRVYADSGSPGVKL-VGNDRVFVRGAQDTAQHLLRLRLMPADLEYIAGHD 119 E +N RR Y ++G V L DR+F + Q L L D+ +IAGHD Sbjct: 60 KLEKVNADLIRRAYGENGMAVVTLDERGDRIFAGSNKGGVQSRLTLAFQENDISFIAGHD 119 Query: 120 VCHTSCYSSIERELPVIKEHCDISFDFSTRTDEDYLGQVCPHIKYAFFSGAELDSVQIEA 179 + HTS YS IER+LP++ H ISFDFS + DE YL VCP++ YAFFSG++L + Sbjct: 120 LLHTSVYSRIERDLPMLSGHVPISFDFSVKRDEAYLEAVCPYVTYAFFSGSDLSESECVR 179 Query: 180 LIKTCHRLGTEIVGITKGSEGALFSKNGVLYKQGVTPAEVVDTMGAGDSFIAGFLVSYLK 239 L + G +IV +T+G GA+ S +Y Q V A+++DT+GAGDSFIAGFL + + Sbjct: 180 LAEKARGFGAKIVCLTRGEYGAVLSDGERVYHQPVVRADIIDTLGAGDSFIAGFLTDFFE 239 Query: 240 KGVMEEALQSGANSAAYTCTFYGGFGYPKP 269 + AL + A +AA TC +G FGY P Sbjct: 240 HQDIRRALTAAAGTAAKTCGGFGAFGYGHP 269 >C1D442.1 C1D442_DEIDV Putative fructoselysine kinase Length = 276 Score = 183 bits (465), Expect = 1e-44, Method: Compositional matrix adjust. Identities = 104/270 (38%), Positives = 144/270 (53%), Gaps = 5/270 (1%) Query: 2 RMIAVGDNVADCYLDQGLYYPGGNAVNVAVNCKRNGCEEVSYIGVFGNDKKSDHIMDSLD 61 R++ +GDN D YL G YPGGNAVNVAV R G SY+G G D + I+ +L Sbjct: 3 RLLGIGDNTVDLYLSDGTMYPGGNAVNVAVLSARLG-HSASYVGCVGTDAAGELILSALR 61 Query: 62 KEGINYRYSRRVYADSGSPGVKLVGNDRVFVRGAQDTAQHLLRLRLMPADLEYIAGHDVC 121 EG++ + R++ + G++ DR FV A L ADL + A HD+ Sbjct: 62 AEGVDLSHCRQMEGMTSWSGIEHRDGDRYFVGSAPGVQG---EWTLTEADLTFTAAHDLV 118 Query: 122 HTSCYSSIERELPVIKEHCDI-SFDFSTRTDEDYLGQVCPHIKYAFFSGAELDSVQIEAL 180 HTS YS ++ LP I++ + S+DFS+ D L +V P + AF S E + AL Sbjct: 119 HTSIYSRLDDALPRIRQAAALLSYDFSSEWTTDLLARVAPQLDVAFLSAGEDPLPEATAL 178 Query: 181 IKTCHRLGTEIVGITKGSEGALFSKNGVLYKQGVTPAEVVDTMGAGDSFIAGFLVSYLKK 240 + G +V +T+G+ GAL + VLY Q PA VVDT+GAGD+FI FL + Sbjct: 179 AEEVAGYGAGVVVVTRGARGALALTDTVLYTQNPVPAAVVDTLGAGDAFITAFLHCWATH 238 Query: 241 GVMEEALQSGANSAAYTCTFYGGFGYPKPI 270 + AL +GA+ AA C +G FGY PI Sbjct: 239 HDVPAALTAGAHEAARNCAVHGAFGYGTPI 268 >Q44323.1 Q44323_9RHIZ MocE Length = 272 Score = 171 bits (433), Expect = 6e-41, Method: Compositional matrix adjust. Identities = 101/272 (37%), Positives = 150/272 (55%), Gaps = 8/272 (2%) Query: 1 MRMIAVGDNVADCYLDQGLYYPGGNAVNVAVNCKRNGCEEVSYIGVFGNDKKSDHIMDSL 60 MR+ VGDNV D Y +Q L +PGGNAVN AV+ +R+G + +YIGV G D D I SL Sbjct: 1 MRVCGVGDNVVDRYFNQKLMFPGGNAVNFAVHAQRSGMD-AAYIGVIGTDADGDLIRSSL 59 Query: 61 DKEGINYRYSRRVYADSGSPGVKLV--GNDRVFVRGAQDTAQHLLRLRLMPADLEYIAGH 118 EG+ ++ R + V + GN+RV+ G + + +L ADLEY+ Sbjct: 60 QAEGVELKHLRVKEGPNAFATVHMDEDGNNRVW--GLCEKGVSMFQLD--TADLEYLGKF 115 Query: 119 DVCHTSCYSSIERELPVIKEHCDISFDFSTRTDEDYLGQVCPHIKYAFFSGAELDSVQIE 178 D+ HT S ++ +LP ++E ISFDFS R+ DY V P+IK A FS ++ +++ Sbjct: 116 DLAHTGETSQLDSQLPAMRERVAISFDFSDRS-LDYAASVLPYIKIAAFSRSDASDDEVK 174 Query: 179 ALIKTCHRLGTEIVGITKGSEGALFSKNGVLYKQGVTPAEVVDTMGAGDSFIAGFLVSYL 238 ++ T G E+V +T+G+ GA G + P + VDT+GAGD+F++ L Sbjct: 175 RVLDTAVSAGVELVTVTQGARGATVCHRGNVLFAPAVPVDAVDTLGAGDAFLSRLACRVL 234 Query: 239 KKGVMEEALQSGANSAAYTCTFYGGFGYPKPI 270 + EA + GA AA+ C G FG+ +PI Sbjct: 235 AGVTLNEAARDGAQYAAHICGTRGAFGHARPI 266 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.138 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,698,198,116 Number of extensions: 73280606 Number of successful extensions: 157931 Number of sequences better than 1.0e-05: 188 Number of HSP's gapped: 158263 Number of HSP's successfully gapped: 188 Length of query: 272 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 139 Effective length of database: 1,807,701,208 Effective search space: 251270467912 Effective search space used: 251270467912 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)