BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0254.0005.p (328 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q2B7W1.1 Q2B7W1_9BACI Putative uncharacterized protein 352 2e-95 B7CD74.1 B7CD74_9FIRM Putative uncharacterized protein 324 7e-87 O32157.1 O32157_BACSU Fructoselysine-6-P-deglycase EC=3.5.-.- 322 3e-86 A7Z8H2.1 A7Z8H2_BACA2 YurP 313 2e-83 B9K3K5.1 B9K3K5_AGRVS YurP 277 1e-72 >Q2B7W1.1 Q2B7W1_9BACI Putative uncharacterized protein Length = 324 Score = 352 bits (904), Expect = 2e-95, Method: Compositional matrix adjust. Identities = 172/328 (52%), Positives = 225/328 (68%), Gaps = 4/328 (1%) Query: 1 MNQEHVQQLEAAIQAVAQRKVSNFYFVACGGSLANVTPAQYILDREIEIPSAAYSSNEFI 60 M QEH Q++ AI+A+ + V N +FVACGGS+A+++ Y + ++ PS Y+SNEF+ Sbjct: 1 MKQEHTNQIQMAIEAIENKNVQNVFFVACGGSMASLSFGDYFITKDTGKPSFVYTSNEFV 60 Query: 61 HRSPKALGPSSVVITCSHSGNTPETVAATTFAREKGALTICLTNLVDSPLWEAAEFKLHY 120 H +PKAL +S+VI SHSG TPETV A T+AR KGALT+ ++ V+SPL + AE+ LHY Sbjct: 61 HLNPKALNENSLVILRSHSGTTPETVKAATYARSKGALTVAISMDVESPLCKEAEYVLHY 120 Query: 121 NYAPYGEALRGEETSMAILYRLVFGILQTLYPNEKYANAISSIENGLHGVYERNKALTLD 180 NY +A+ GE + I Y +F +L+ +EKY I + N L + E+N+ L + Sbjct: 121 NYKDGSDAIDGE---IGIYYTFIFKLLKIFTGDEKYERGIEQLSN-LGDLIEKNQQLFRE 176 Query: 181 AAKAFGRNYKREKLIYTMASGINYSVAYSFAICLLQEMQWIHSSAIHSGEYFHGPFEITD 240 AA FG+ KREK IYTMASG AYSF CLL EM WIHS+AIHSGEYFHGPFEITD Sbjct: 177 AAFEFGKRNKREKTIYTMASGAYIHHAYSFTSCLLMEMLWIHSNAIHSGEYFHGPFEITD 236 Query: 241 FDVPFIVIKGLGETRPLDDRAYDFCVKYSDKTILIDAETFDLEGISPDVRPYFTNLVAGV 300 FDVPF++I G G +RPLD+RA DF KYS+K ++DA F+ G+ D++ YF + G Sbjct: 237 FDVPFLLIAGEGPSRPLDERALDFVAKYSEKVEVVDASKFNYSGVDEDLKEYFGPALTGP 296 Query: 301 VLRQYAQHLSEETGHALSVRRYMWKMEY 328 V+R YA L+E TGH LSVRRYMWKMEY Sbjct: 297 VMRLYADGLAEHTGHPLSVRRYMWKMEY 324 >B7CD74.1 B7CD74_9FIRM Putative uncharacterized protein Length = 325 Score = 324 bits (830), Expect = 7e-87, Method: Compositional matrix adjust. Identities = 154/329 (46%), Positives = 220/329 (66%), Gaps = 5/329 (1%) Query: 1 MNQEHVQQLEAAIQAVAQRK-VSNFYFVACGGSLANVTPAQYILDREIEIPSAAYSSNEF 59 M H Q+ A++AVA RK V+ YFVACGGS A + QY+ D+E +PS Y++NEF Sbjct: 1 MRNLHELQITTAVEAVASRKEVNEVYFVACGGSQAVLMAGQYLFDKESSVPSHVYTANEF 60 Query: 60 IHRSPKALGPSSVVITCSHSGNTPETVAATTFAREKGALTICLTNLVDSPLWEAAEFKLH 119 ++ +PK L SVVI+CSHSGNTPETVAAT AR KGA+TICL+NL SPLWEAAE+ +H Sbjct: 61 VYDTPKNLCEKSVVISCSHSGNTPETVAATKLARSKGAITICLSNLEGSPLWEAAEYPVH 120 Query: 120 YNYAPYGEALRGEETSMAILYRLVFGILQTLYPNEKYANAISSIENGLHGVYERNKALTL 179 Y++ G+ + + + ILY L+F +L L + K+ +E L + + K Sbjct: 121 YDW---GKDVSDSDKNKGILYGLLFKLLGVLAADAKWDICFKELEK-LTELSTKAKEQYD 176 Query: 180 DAAKAFGRNYKREKLIYTMASGINYSVAYSFAICLLQEMQWIHSSAIHSGEYFHGPFEIT 239 AK + ++ KR+K IYT+ SGINY YS A+C EMQWI+S IHSGE+FHGPFE+T Sbjct: 177 AQAKQWAKDNKRKKTIYTIGSGINYGEVYSTAMCWFMEMQWINSGCIHSGEFFHGPFEVT 236 Query: 240 DFDVPFIVIKGLGETRPLDDRAYDFCVKYSDKTILIDAETFDLEGISPDVRPYFTNLVAG 299 D+DVPF+++K +G TR LD+R +F K+++ +++D + DL ++ + R Y ++ G Sbjct: 237 DYDVPFMLVKSIGNTRFLDERVENFAKKFTEDLLVLDQKDLDLSNVAEEARQYVAAILTG 296 Query: 300 VVLRQYAQHLSEETGHALSVRRYMWKMEY 328 VV+R + + ++ E GH+L VRRYMW+MEY Sbjct: 297 VVIRHFVEAIAFERGHSLDVRRYMWQMEY 325 >O32157.1 O32157_BACSU Fructoselysine-6-P-deglycase EC=3.5.-.- Length = 328 Score = 322 bits (825), Expect = 3e-86, Method: Compositional matrix adjust. Identities = 161/323 (49%), Positives = 215/323 (66%), Gaps = 5/323 (1%) Query: 7 QQLEAAIQAVAQRKVSNFYFVACGGSLANVTPAQYILDREIE-IPSAAYSSNEFIHRSPK 65 ++++A +Q + + + + +FVACGGS A + P++Y+ DRE + I S YS+NEFI R+P Sbjct: 10 REVQAFLQDLKGKTIDHVFFVACGGSSAIMYPSKYVFDRESKSINSDLYSANEFIQRNPV 69 Query: 66 ALGPSSVVITCSHSGNTPETVAATTFAREKGALTICLTNLVDSPLWEAAEFKLHYNYAPY 125 LG S+VI CSHSGNTPETV A FAR KGALTI +T +SPL + A++ Y++ Sbjct: 70 QLGEKSLVILCSHSGNTPETVKAAAFARGKGALTIAMTFKPESPLAQEAQYVAQYDW--- 126 Query: 126 GEALRGEETSMAILYRLVFGILQTLYPNEKYANAISSIENGLHGVYERNKALTLDAAKAF 185 G+ T+ +LY++VFG LQ L N K+ AI ++ L VYE+ D AK F Sbjct: 127 GDEALAINTNYGVLYQIVFGTLQVLENNTKFEQAIEGLDQ-LQAVYEKALKQEADNAKQF 185 Query: 186 GRNYKREKLIYTMASGINYSVAYSFAICLLQEMQWIHSSAIHSGEYFHGPFEITDFDVPF 245 + +++E +IYTMASG NY VAYS++IC+L EMQWIHS AIH+GEYFHGPFEI D VPF Sbjct: 186 AKAHEKESIIYTMASGANYGVAYSYSICILMEMQWIHSHAIHAGEYFHGPFEIIDESVPF 245 Query: 246 IVIKGLGETRPLDDRAYDFCVKYSDKTILIDAETFDLEGISPDVRPYFTNLVAGVVLRQY 305 I++ GL ETRPL++RA F KY K ++DA ++D I V+ Y LV VLR Y Sbjct: 246 IILLGLDETRPLEERALTFSKKYGKKLTVLDAASYDFTAIDDSVKGYLAPLVLNRVLRSY 305 Query: 306 AQHLSEETGHALSVRRYMWKMEY 328 A L+EE H LS RRYMWK+EY Sbjct: 306 ADELAEERNHPLSHRRYMWKVEY 328 >A7Z8H2.1 A7Z8H2_BACA2 YurP Length = 328 Score = 313 bits (801), Expect = 2e-83, Method: Compositional matrix adjust. Identities = 159/323 (49%), Positives = 212/323 (65%), Gaps = 5/323 (1%) Query: 7 QQLEAAIQAVAQRKVSNFYFVACGGSLANVTPAQYILDREIE-IPSAAYSSNEFIHRSPK 65 +++A + + + + + +FVACGGS A + P++Y+ DRE + I S YSSNEFI R+P Sbjct: 10 HKVQAFLDDLKGKTIDHVFFVACGGSSAIMYPSKYVFDREAKAISSDLYSSNEFIQRNPV 69 Query: 66 ALGPSSVVITCSHSGNTPETVAATTFAREKGALTICLTNLVDSPLWEAAEFKLHYNYAPY 125 LG +S+VI CS SGNTPETV A FAR+KGALTI +T DSPL + AE+ Y++ Sbjct: 70 QLGENSLVILCSQSGNTPETVKAAGFARKKGALTIAMTFEPDSPLAKEAEYVAVYDW--- 126 Query: 126 GEALRGEETSMAILYRLVFGILQTLYPNEKYANAISSIENGLHGVYERNKALTLDAAKAF 185 GE + + +LY++VFG L L + + AI + N L VY++ D AKAF Sbjct: 127 GENAQAINMNYGVLYQIVFGALHVLENHPVFEQAIDGL-NKLQTVYDKALKQEADHAKAF 185 Query: 186 GRNYKREKLIYTMASGINYSVAYSFAICLLQEMQWIHSSAIHSGEYFHGPFEITDFDVPF 245 + +++E +IYTMASG NY VAYS++IC+L EMQWIHS AIH+GEYFHGPFEI D VPF Sbjct: 186 AKAHEKEDIIYTMASGANYGVAYSYSICILMEMQWIHSHAIHAGEYFHGPFEIIDESVPF 245 Query: 246 IVIKGLGETRPLDDRAYDFCVKYSDKTILIDAETFDLEGISPDVRPYFTNLVAGVVLRQY 305 I++ GL ETRPL++RA F +Y K ++DA +D GI V+ Y LV VLR Y Sbjct: 246 IILLGLDETRPLEERALSFSKRYGKKLTVLDAAAYDFSGIDDSVKGYLAPLVLNRVLRSY 305 Query: 306 AQHLSEETGHALSVRRYMWKMEY 328 A L+E H LS RRYMWK+EY Sbjct: 306 ADALAEARNHPLSQRRYMWKVEY 328 >B9K3K5.1 B9K3K5_AGRVS YurP Length = 327 Score = 277 bits (708), Expect = 1e-72, Method: Compositional matrix adjust. Identities = 158/327 (48%), Positives = 211/327 (64%), Gaps = 10/327 (3%) Query: 8 QLEAAIQ----AVAQRK-VSNFYFVACGGSLANVTPAQYILDREIE-IPSAAYSSNEFIH 61 Q+E++IQ AVAQRK VS YFVACGGS A + +Y +DR I + ++S EFI Sbjct: 5 QIESSIQSALAAVAQRKSVSTIYFVACGGSFAQMHLPKYAVDRNSSTIFAETFNSAEFIA 64 Query: 62 RSPKALGPSSVVITCSHSGNTPETVAATTFAREKGALTICLTNLVDSPLWEAAEFKLHYN 121 R+P LG SVVI CS SGNTPETVAA FA++KGA TI LT +S L + ++ + Y Sbjct: 65 RNPVHLGEGSVVILCSSSGNTPETVAAAAFAKKKGAFTIGLTTKPESELGQVSDSTVPYV 124 Query: 122 YAPYGEALRGEETSMAILYRLVFGILQTLYPNEKYANAISSIENGLHGVYERNKALTLDA 181 P + ++ I+ R+ FGI+ + K A +S++E + + + + + Sbjct: 125 STPL---IGNQDAPSGIILRIAFGIVNARENSPKAAALLSALEK-VGDIAASAQKVHAED 180 Query: 182 AKAFGRNYKREKLIYTMASGINYSVAYSFAICLLQEMQWIHSSAIHSGEYFHGPFEITDF 241 A+ +G KRE IYTMASG N+ VAYSF+IC+LQEMQWI+S AIHSGEYFHGPFEITDF Sbjct: 181 AREWGERNKREPTIYTMASGSNWGVAYSFSICILQEMQWINSQAIHSGEYFHGPFEITDF 240 Query: 242 DVPFIVIKGLGETRPLDDRAYDFCVKYSDKTILIDAETFDLEGISPDVRPYFTNLVAGVV 301 D P +++ GLG TR +D RA F KYS +++DA D+ G++P+V Y T LV + Sbjct: 241 DTPILLLVGLGPTREMDTRAQKFAEKYSKNLLVLDAAKIDMHGVAPEVAEYITPLVFQPL 300 Query: 302 LRQYAQHLSEETGHALSVRRYMWKMEY 328 +R YA L+E GH L+VRRYMWKMEY Sbjct: 301 MRIYAVELAERRGHPLTVRRYMWKMEY 327 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.134 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,951,269,193 Number of extensions: 80261743 Number of successful extensions: 199194 Number of sequences better than 1.0e-05: 1343 Number of HSP's gapped: 198291 Number of HSP's successfully gapped: 1343 Length of query: 328 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 192 Effective length of database: 1,779,438,163 Effective search space: 341652127296 Effective search space used: 341652127296 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)