BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0251.0034.p (312 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C0EFB9.1 C0EFB9_9CLOT Putative uncharacterized protein 189 3e-46 A8ME37.1 A8ME37_CALMQ Putative esterase 182 3e-44 B1D6H6.1 B1D6H6_9BACL Acetyl esterase family enzyme 171 9e-41 A5ZN29.1 A5ZN29_9FIRM Putative uncharacterized protein 167 9e-40 A1A0I6.1 A1A0I6_BIFAA Possible xylan esterase 166 3e-39 >C0EFB9.1 C0EFB9_9CLOT Putative uncharacterized protein Length = 277 Score = 189 bits (480), Expect = 3e-46, Method: Compositional matrix adjust. Identities = 112/320 (35%), Positives = 161/320 (50%), Gaps = 56/320 (17%) Query: 2 MICKKIPIC---------ENKEEVFMEAYILHNSVEFNHNLKRPVALIIPGGAYISTSDR 52 M+C++IP+ N + ++AY++ EFN KRP ++ PGG Y TSDR Sbjct: 1 MVCQQIPVVVDYAKAGAVHNGFQPVLQAYVIERGPEFNVLQKRPAVILCPGGGYGFTSDR 60 Query: 53 EAEQVALAFATHGYHAFVLRYSIGKEALMPQPILEGFKAISIIRDHADEWCVDVNRIVPC 112 EAE VAL + G +AFVLRYS+ A P +LE +A+ ++R +A++W +D NR++ C Sbjct: 61 EAEPVALRLVSAGINAFVLRYSVAP-ARYPCALLELARAVELVRQNAEKWHIDRNRVIVC 119 Query: 113 GFSAGGHLAASLATMWNLPEFANALGCSSESIKPNAVVLSYPCVVVPN--HAGSLDTGMP 170 GFSAGGHLAA+L W P LG S ++PN ++LSYP + H GS Sbjct: 120 GFSAGGHLAANLGVAWEQPFLCETLGAKSRDLRPNGMILSYPVITAGEYAHIGS------ 173 Query: 171 AEALEAFKANPMMSELNEALVVKDGTIRLDLIDVMYRNMTGKPDWKAEDLQ-SYSTDLLV 229 + N+ G D K E+L S S + LV Sbjct: 174 -----------------------------------FENLLG--DTKNEELMASVSLEKLV 196 Query: 230 SEDTVPSFVWATENDILVPVSGAIQFVQAMLKNGRRIEFHLFAGGEHGLSLAKATTAGSP 289 + T PSF+W T+ D +VP+ + F A+ N E H++ G HGLSLA T+A P Sbjct: 197 NRSTPPSFLWHTQADPVVPLENTVLFAMALRNNKVPFELHIYERGGHGLSLADETSAVLP 256 Query: 290 NMVNDEVAVWFNMAIAFLAK 309 + +V W MAI +L + Sbjct: 257 RHIVPDVTNWIEMAIKWLKR 276 >A8ME37.1 A8ME37_CALMQ Putative esterase Length = 287 Score = 182 bits (463), Expect = 3e-44, Method: Compositional matrix adjust. Identities = 113/308 (36%), Positives = 162/308 (52%), Gaps = 33/308 (10%) Query: 10 CENKEEVFMEAYILHNSVEFNHNLKRPVALIIPGGAYISTSDREAEQVALAFATHGYHAF 69 + V + +YIL S E + +RP +I PGG ++ TSDREAE VA F + GYHAF Sbjct: 8 LRDDRPVTLTSYILDTSPEISWG-RRPAIIICPGGGFVRTSDREAEPVASIFLSRGYHAF 66 Query: 70 VLRYS---IGKEALMPQPILEGFKAISIIRDHADEWCVDVNRIVPCGFSAGGHLAASLAT 126 VLRYS +G + P ++E A+ IR +AD+W +D RI GFSAGG +AA + Sbjct: 67 VLRYSTESMGVSKVYPDVVIELANAVVSIRRNADKWNIDPGRIAIIGFSAGGTVAALYSV 126 Query: 127 MWNLPEFANALGCSSESIKPNAVVLSYPCV--VVPNHAGSLDTGMPAEALEAFKANPMMS 184 W+ + + +++KP+AV+L+YP V V N + PA + FK MMS Sbjct: 127 NWHRDWLSKLVNAPKDTLKPSAVILAYPVVDFAVMNEVTKNNRNTPAAGV-LFK---MMS 182 Query: 185 ELNEALVVKDGTIRLDLIDVMYRNMTGKPDWKAEDLQSYSTDLLVSEDTVPSFVWATEND 244 L + G D DL+ S V E+T P+F+W T +D Sbjct: 183 -----LALGSGKFTED------------------DLRELSATYHVDENTPPTFIWTTADD 219 Query: 245 ILVPVSGAIQFVQAMLKNGRRIEFHLFAGGEHGLSLAKATTAGSPNMVNDEVAVWFNMAI 304 +VPV I +V A+ +N EFH+F G HGLSLA TTA +P VN VA W +A+ Sbjct: 220 DVVPVESIISYVNALARNKVPFEFHVFEKGVHGLSLADKTTASNPQHVNPPVAKWIELAL 279 Query: 305 AFLAKTLD 312 ++L + ++ Sbjct: 280 SWLERHIE 287 >B1D6H6.1 B1D6H6_9BACL Acetyl esterase family enzyme Length = 267 Score = 171 bits (433), Expect = 9e-41, Method: Compositional matrix adjust. Identities = 105/285 (36%), Positives = 145/285 (50%), Gaps = 45/285 (15%) Query: 23 LHNSVEFNHNLKRPVALIIPGGAYISTSDREAEQVALAFATHGYHAFVLRYSIGKEALMP 82 LH+ VE RP+ L+ PGGAY S+REAE VA+AF GYHA VLRYS ++A Sbjct: 20 LHDIVELREK-ARPIVLVTPGGAYRFCSEREAEPVAIAFYNAGYHAAVLRYSCMEDARNM 78 Query: 83 QPILEGFKAISIIRDHADEWCVDVNRIVPCGFSAGGHLAASLATMWNLPEFANALGCSSE 142 QP+ E ++I I+R+HAD+W + ++I CGFSAGGHLAAS TMWN + L + + Sbjct: 79 QPMAEALESIRILREHADKWHIAEDKIAVCGFSAGGHLAASTGTMWNESALMDRLNYAGD 138 Query: 143 SIKPNAVVLSYPCVVVPNHAGSLDTGMPAEALEAFKANPMMSELNEALVVKDGTIRLDLI 202 +KPNA++L YP + A E+F Sbjct: 139 ILKPNALILGYPVITSGEFAHR----------ESFY------------------------ 164 Query: 203 DVMYRNMTGKPDWKAEDLQSYSTDLLVSEDTVPSFVWATENDILVPVSGAIQFVQAMLKN 262 N++G + E YS + V+ +T P+F+W TE+D V V A+ F+ A+ K+ Sbjct: 165 -----NISGSRE-DDEISGFYSLEKRVTSETPPTFLWHTEDDPAVTVENALLFISALRKH 218 Query: 263 GRRIEFHLFAGGEHGLSLAKATTAGSPNMVNDEVAVWFNMAIAFL 307 E H+F GEHGLSL N N A WF + I +L Sbjct: 219 QVPFELHIFNKGEHGLSLCNEEV----NTPNPHCAHWFGLCIEWL 259 >A5ZN29.1 A5ZN29_9FIRM Putative uncharacterized protein Length = 272 Score = 167 bits (424), Expect = 9e-40, Method: Compositional matrix adjust. Identities = 106/311 (34%), Positives = 153/311 (49%), Gaps = 47/311 (15%) Query: 2 MICKKIPI-CENKEEVF-MEAYILHNSVEFNHNLKRPVALIIPGGAYISTSDREAEQVAL 59 MI KK+ I E +E + YIL NSVE + KRPV +I PGG Y TSDREAE +A+ Sbjct: 1 MIYKKVEIKVEGYQETADLYTYILDNSVEMRPDRKRPVIVICPGGGYTMTSDREAEPIAM 60 Query: 60 AFATHGYHAFVLRYSIGKEALMPQPILEGFKAISIIRDHADEWCVDVNRIVPCGFSAGGH 119 + GYHA +LRYS+ A P +L+ K+++ +R+HA+E+ +D +I+ GFSAG H Sbjct: 61 QYLARGYHAVILRYSVAP-ARYPLALLQLAKSVAYLREHAEEFHIDTAKIILQGFSAGAH 119 Query: 120 LAASLATMWNLPEFANALGCSSESIKPNAVVLSYPCVVVPN--HAGSLDTGMPAEALEAF 177 LAASL W + L +E ++PN ++LSYP + H GS Sbjct: 120 LAASLGVFWKKKFISEVLHTETEMVRPNGMILSYPVISSGKFAHTGSF------------ 167 Query: 178 KANPMMSELNEALVVKDGTIRLDLIDVMYRNMTGKPDWKAEDLQSYSTDLLVSEDTVPSF 237 EAL+ D + E + S + V+ DT P+F Sbjct: 168 ----------EALLGADSS-------------------DPEKRKKQSLEFQVTTDTPPTF 198 Query: 238 VWATENDILVPVSGAIQFVQAMLKNGRRIEFHLFAGGEHGLSLAKATTAGSP-NMVNDEV 296 +W T D VPV ++ F +A+ K +E HL+ G HGLSLA T+ + E Sbjct: 199 LWHTVTDDCVPVENSLLFFEALHKYKVPVELHLYPTGVHGLSLANEETSHEDGGCIQPEC 258 Query: 297 AVWFNMAIAFL 307 W +A ++ Sbjct: 259 QSWMELACTWI 269 >A1A0I6.1 A1A0I6_BIFAA Possible xylan esterase Length = 277 Score = 166 bits (420), Expect = 3e-39, Method: Compositional matrix adjust. Identities = 111/309 (35%), Positives = 154/309 (49%), Gaps = 47/309 (15%) Query: 6 KIPICENKEEVFMEAYILHNSVEFNHNLKRPVALIIPGGAYISTSDREAEQVALAFATHG 65 KIP ++ F+ Y L NS E + + +RP L+IPGG Y TSDREAE +A+ F G Sbjct: 11 KIPGIDDSTATFV-GYCLDNSKEIDPDRRRPSVLVIPGGGYEMTSDREAEPIAMQFLAAG 69 Query: 66 YHAFVLRYSIGKEALMPQPILEGFKAISIIRDHADEWCVDVNRIVPCGFSAGGHLAASLA 125 ++AFVLRYS+ K ++ P +LE +A+ +R HA EW VD +RI GFSAGGHLAA+LA Sbjct: 70 FNAFVLRYSV-KPSVFPVALLEAAEAVRRMRAHAAEWHVDGDRIAVIGFSAGGHLAANLA 128 Query: 126 TMWNLPEFANALGCSSESIKPNAVVLSYPCVVVP--NHAGSLDTGMPAEALEAFKANPMM 183 T + A A G ++++PNA++L+YP + H GS D Sbjct: 129 TSASDDVLA-AHGYDPDAVRPNALMLAYPVITSGPLAHRGSFDA---------------- 171 Query: 184 SELNEALVVKDGTIRLDLIDVMYRNMTGKPDWKAEDLQSYSTDLLVSEDTVPSFVWATEN 243 + G AE L+ S + + T P FVW T Sbjct: 172 -------------------------LLGDRKADAEALEPVSIEKHIDAKTPPVFVWHTVP 206 Query: 244 DILVPVSGAIQFVQAMLKNGRRIEFHLFAGGEHGLSLAKATTA-GSPNMVNDEVAVWFNM 302 D VP + + A K G IE HLF G HGL+L A TA N + + V W + Sbjct: 207 DDCVPYENTLMLIDACKKAGVSIEAHLFPSGGHGLALGTAETAWQGVNGIEECVQAWPKL 266 Query: 303 AIAFLAKTL 311 A+A++ +TL Sbjct: 267 AVAWMRRTL 275 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.134 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,859,762,393 Number of extensions: 75851840 Number of successful extensions: 171158 Number of sequences better than 1.0e-05: 264 Number of HSP's gapped: 171249 Number of HSP's successfully gapped: 306 Length of query: 312 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 177 Effective length of database: 1,788,859,178 Effective search space: 316628074506 Effective search space used: 316628074506 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)