BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0251.0031.p (330 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A6BF68.1 A6BF68_9FIRM Putative uncharacterized protein 189 4e-46 C4Z067.1 C4Z067_9FIRM 2-isopropylmalate synthase 150 2e-34 A5ZM13.1 A5ZM13_9FIRM Putative uncharacterized protein 115 4e-24 A8S1Q1.1 A8S1Q1_9CLOT Putative uncharacterized protein 109 3e-22 C0EG54.1 C0EG54_9CLOT Putative uncharacterized protein 105 4e-21 >A6BF68.1 A6BF68_9FIRM Putative uncharacterized protein Length = 348 Score = 189 bits (479), Expect = 4e-46, Method: Compositional matrix adjust. Identities = 93/256 (36%), Positives = 158/256 (61%), Gaps = 1/256 (0%) Query: 63 RFKDRHRHDYVELAYVCKGQLPMEISGNHYIFQRDEVFIIDRNCIHSEDLTNTDSAVVFL 122 ++ H HDY+EL+YV +G+ I +FQ+ ++ +ID+NC+H + LT+ V+F+ Sbjct: 85 KYTQLHTHDYLELSYVVEGEFHQRILNKDVVFQKGDLCLIDKNCLHQDCLTDQSGVVLFI 144 Query: 123 CMSETFFDEIFLSELQEEPLQEFIRMCLKKQKRIRQFMRFTPVSDNNQMYNLISQVV-EE 181 ++ F EI + + F++ L KQK ++QF+ F P ++ + ++ +E Sbjct: 145 GIANDMFTEIMNENSTPQKILSFLQSALLKQKDVQQFLHFRPSDGASESLDDSLLLLLKE 204 Query: 182 AESKRKGYDYLLKGLMMRIMDVLANSYQIQLSKLEKQRKDEILFQELEKYLHQKYKEVTI 241 + S G Y+ KGL+ RI +L+ Y LSK +KQ + I+F+E+ Y+ ++++TI Sbjct: 205 SYSPDSGSRYITKGLLFRIFRILSTQYDFSLSKEQKQTMNWIVFEEISDYIRAHFRDITI 264 Query: 242 EELVLKYHYNADYFNRLIKKYTQLSFSQFLQTIRLSKAEEMLVASKLPVHQIIHEVGYQN 301 ++LV ++HY DYFNRLIK+ T L++S ++Q IRL +AE +LV++ + +I VGY N Sbjct: 265 QDLVDEFHYQEDYFNRLIKRKTGLTYSAYVQQIRLERAEHLLVSTDKSIEEIADSVGYHN 324 Query: 302 KGYFYKIFVQRYGITP 317 KGYFYK+F ++Y TP Sbjct: 325 KGYFYKLFQEKYEETP 340 >C4Z067.1 C4Z067_9FIRM 2-isopropylmalate synthase Length = 300 Score = 150 bits (378), Expect = 2e-34, Method: Compositional matrix adjust. Identities = 77/253 (30%), Positives = 145/253 (57%), Gaps = 2/253 (0%) Query: 66 DRHRHDYVELAYVCKGQLPMEISGNHYIFQRDEVFIIDRNCIHSEDLTNTDSAVVFLCMS 125 D ++H Y EL YV G L + G+ ++ +V ID C+H E D+ V+ L +S Sbjct: 35 DMYKHGYAELLYVSDGSLTEHVCGDKVHIKKGDVCFIDSGCVHKESFEEDDTFVIRLEVS 94 Query: 126 ETFFDEIFLSELQEEPLQEFIRMCLKKQKRIRQFMRFTPVSD-NNQMYNLISQVVEEAES 184 + + + + + ++ ++I+ +K K ++ F D +N++ ++++ ++ E Sbjct: 95 DEIINAVISNAMADKQAVDYIKELVKVLKN-SGYIHFGYKDDYDNELKDMLTAMISECGV 153 Query: 185 KRKGYDYLLKGLMMRIMDVLANSYQIQLSKLEKQRKDEILFQELEKYLHQKYKEVTIEEL 244 Y+ +GLM+RI+ L Y+ S+ +++ + +L QE+ ++ K+VTI+ L Sbjct: 154 ADMASAYICQGLMLRILWRLGTVYEYAQSRYIRKKINWLLHQEITDFIEDNMKDVTIDML 213 Query: 245 VLKYHYNADYFNRLIKKYTQLSFSQFLQTIRLSKAEEMLVASKLPVHQIIHEVGYQNKGY 304 V ++ Y DYFNR +K T ++F+++LQ RL+K+ +L + V +II +VGY+NKGY Sbjct: 214 VERFGYQEDYFNRFLKAQTGMTFTEYLQDCRLNKSAILLKEDSMDVDEIIKKVGYRNKGY 273 Query: 305 FYKIFVQRYGITP 317 FY+IF ++Y +TP Sbjct: 274 FYRIFTEKYNMTP 286 >A5ZM13.1 A5ZM13_9FIRM Putative uncharacterized protein Length = 327 Score = 115 bits (289), Expect = 4e-24, Method: Compositional matrix adjust. Identities = 74/252 (29%), Positives = 131/252 (51%), Gaps = 4/252 (1%) Query: 68 HRHDYVELAYVCKGQLPMEISGNHYIFQRDEVFIIDRNCIHS-EDLTNTDSAVVFLCMSE 126 HRH+Y+E+ YVC+GQL I G + E+ +++ H D A+ F+ + E Sbjct: 65 HRHNYIEVLYVCQGQLTNIIGGKEVVIHAGELLFLNQYTRHEILPAGEEDIAINFMILPE 124 Query: 127 TFFDEIFLSELQEEPLQEFIRMCLKKQKRIRQFMRFTPVSDNNQMYNLISQVVEEAESKR 186 FFD + L +F+ L++ + +++ F V++ Q+ NL+ ++ + + Sbjct: 125 -FFDVAYTMAGNNNVLADFLVNVLRQDEERGEYLHF-KVAEVLQIQNLLENMIYSLVTGK 182 Query: 187 KGYDYLLKGLMMRIMDVLANSYQIQLSKLEKQRKDEILFQELEKYLHQKYKEVTIEELVL 246 + + + M I L S Q +L Q ++ I+ L+ Y+ Q+YK T+ EL Sbjct: 183 GDQNRINQTTMGLIFLYLLESVQYAEMRLPNQYENMIIMTTLD-YIEQQYKTATLTELCE 241 Query: 247 KYHYNADYFNRLIKKYTQLSFSQFLQTIRLSKAEEMLVASKLPVHQIIHEVGYQNKGYFY 306 K H +++I+K T +F + LQ RL+KA E++ + LP+ II VGY+N YF+ Sbjct: 242 KLHLPMHMLSKMIRKNTGFNFKELLQRKRLNKAVELMCETDLPISDIIAAVGYENGSYFH 301 Query: 307 KIFVQRYGITPK 318 ++F +RY TP+ Sbjct: 302 RVFKERYHTTPR 313 >A8S1Q1.1 A8S1Q1_9CLOT Putative uncharacterized protein Length = 322 Score = 109 bits (273), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 74/271 (27%), Positives = 144/271 (53%), Gaps = 11/271 (4%) Query: 56 VDIDPTRRFKD--RHRHDYVELAYVCKGQLPMEISGNHYIFQRDEVFIIDRNCIHS-EDL 112 + I P RF H H+YVE+ Y+C+G+ + G + E+ ++++ + + Sbjct: 51 ITIRPHTRFVHFPEHTHNYVEVIYMCRGETTHVVDGRELKLKEGELLFLNQHAVQEIQPA 110 Query: 113 TNTDSAVVFLCMSETFFDEIFLSELQEEPL-QEFIRMCLKKQKRIRQFMRFTPVSDNNQM 171 D AV F+ + E FFD F +EE L ++FI CL R +++ F ++D + Sbjct: 111 GQDDIAVNFIILPE-FFDTAFRMMGEEENLLRDFIVGCLCDDTRYGRYLHFQ-IADVLPV 168 Query: 172 YNLISQVVEEAESKRKGYDYL---LKGLMMRIMDVLANSYQIQLSKLEKQRKDEILFQEL 228 NL+ +V +R+G + GL++R ++ + +I++++ +Q ++ L ++ Sbjct: 169 QNLVENLVWSLMHEREGMQAMNQTTMGLLLR--HLMHYTGRIRVNRDSEQSFEQQLTLQV 226 Query: 229 EKYLHQKYKEVTIEELVLKYHYNADYFNRLIKKYTQLSFSQFLQTIRLSKAEEMLVASKL 288 +Y+ + Y+E ++ EL Y+ + +R I + ++ + LQ RL++A +L ++++ Sbjct: 227 LRYIDEHYREGSLTELAALMGYDVYWLSRAINRLLGRNYKELLQIKRLNQAAFLLHSTRM 286 Query: 289 PVHQIIHEVGYQNKGYFYKIFVQRYGITPKE 319 PV + VGY N YFY+IF YG++PKE Sbjct: 287 PVADVSFAVGYDNTSYFYRIFRTYYGMSPKE 317 >C0EG54.1 C0EG54_9CLOT Putative uncharacterized protein Length = 346 Score = 105 bits (263), Expect = 4e-21, Method: Compositional matrix adjust. Identities = 71/264 (26%), Positives = 137/264 (51%), Gaps = 22/264 (8%) Query: 68 HRHDYVELAYVCKGQLPMEISGNHYIFQRDEVFIIDRNCIHSEDLTNTDSAVVFLCMSET 127 H+HD+ EL YV +G + + + + Q+ ++ I++ + +H+ V + + Sbjct: 77 HKHDFFELIYVYQGSVINVVDAHEVVLQQGDLCIMNASAVHTLGKLGKQDTVYNILIKPE 136 Query: 128 FFDEIFLSELQE-EPLQEFIRMCLKKQKRIRQFMRFTP---VSDNNQMYNLISQVVEEAE 183 F++ FL + E E + F + ++ R FM F SD+ + Y + +++ E Sbjct: 137 LFNQAFLGLIAENEIISSFFLNSIYQKSISRNFMTFHTQDFPSDDAETY--VHRLIMENH 194 Query: 184 SKRKGYDYLLKGLMMRIMDVLANSYQIQLSKLEKQRKDEILFQELE--------KYLHQK 235 K Y+ K + + ++ + I+L++ K R+D+ EL Y+ Sbjct: 195 FKDIYYN---KSIEISLVGLF-----IELTRGYKHRQDQNSSIELGMSNVSQIIAYISDH 246 Query: 236 YKEVTIEELVLKYHYNADYFNRLIKKYTQLSFSQFLQTIRLSKAEEMLVASKLPVHQIIH 295 YK+++I+++ + Y+ +Y + L+KKYT +FS+ LQ IR + A+E L +P+++I++ Sbjct: 247 YKDISIKQVADHFSYHPNYLSSLLKKYTDKTFSEILQNIRFTAAKEYLQKLDIPINEIVY 306 Query: 296 EVGYQNKGYFYKIFVQRYGITPKE 319 VGY NK +FY+ F Q GITP + Sbjct: 307 MVGYTNKSHFYRTFKQEMGITPAQ 330 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.323 0.138 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,852,015,421 Number of extensions: 76195123 Number of successful extensions: 216819 Number of sequences better than 1.0e-05: 1525 Number of HSP's gapped: 216937 Number of HSP's successfully gapped: 1525 Length of query: 330 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 194 Effective length of database: 1,779,438,163 Effective search space: 345211003622 Effective search space used: 345211003622 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 131 (55.1 bits)