BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0251.0030.p (496 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A8LEC9.1 A8LEC9_FRASN Glycoside hydrolase family 2 sugar binding 546 e-153 A8LE96.1 A8LE96_FRASN Glycoside hydrolase family 2 sugar binding 537 e-150 A8LDF3.1 A8LDF3_FRASN Glycoside hydrolase family 2 sugar binding 511 e-143 A8LEG1.1 A8LEG1_FRASN Glycoside hydrolase family 2 sugar binding 506 e-141 B0RCP1.1 B0RCP1_CLAMS Putative glycosyl hydrolase 465 e-129 >A8LEC9.1 A8LEC9_FRASN Glycoside hydrolase family 2 sugar binding Length = 825 Score = 546 bits (1408), Expect = e-153, Method: Compositional matrix adjust. Identities = 256/449 (57%), Positives = 328/449 (73%), Gaps = 1/449 (0%) Query: 20 VTLPHDAMIAKKRSAQAVSGSKKGYFSDGAYDYVKKFYVPEEYKDKRVTFEFEGVYMHAM 79 V LPHDAM+ +R ++GY+ GAY Y K F+ PE+Y+++RVT EFEGVY +A Sbjct: 33 VRLPHDAMVGLERDGADTEAGQRGYYPSGAYQYRKTFFAPEKYRNRRVTLEFEGVYRNAR 92 Query: 80 VYINGDFAGQHPFGYTNFYIKADRFLKYGAENEIKVVAKSHDDSRWYTGTGIYRNTKIMV 139 V+INGDFA Q +GY+NFY+ D LKYG+ NEI V A S +D+RWY+G G+YRNTK++V Sbjct: 93 VFINGDFAAQRAYGYSNFYVHTDHLLKYGSNNEIVVEAYSGNDTRWYSGGGVYRNTKLIV 152 Query: 140 ADLVHIAVDGVKINATDIESKHAVVVVATDVENEGINPRTVRIVTEIVDADGNSVASDTA 199 DLVH+A+DGVKI ++ AVV VAT+++NE R++ ++TEIVDADG VA DTA Sbjct: 153 GDLVHLALDGVKITTPAVDGDLAVVAVATELQNESPVTRSLEVLTEIVDADGTVVARDTA 212 Query: 200 PFTAFAGEKATSRQRLYIREPKLWNVETPYLYTCISKVMDGEHVLDEETHTFGIRTLSLD 259 P TAF G++ T RQRL + +P+LW V+ PYLY C + V +LD+ET FGIRTL++D Sbjct: 213 PVTAFIGDRLTLRQRLSVPQPELWGVDRPYLYLCRTSVTADGELLDQETTRFGIRTLTID 272 Query: 260 AEDGLRINGEVVKLRGACIHHDNGVIGASTIERADERRIEILKQAGFNAIRSAHHPASKA 319 GLR+NGEVV LRGACIHHDNGVIGA+TI+RA++RR+EILKQAGFNAIRS+H+P SKA Sbjct: 273 PRRGLRVNGEVVNLRGACIHHDNGVIGAATIDRAEQRRVEILKQAGFNAIRSSHNPMSKA 332 Query: 320 LLKACDRIGMLVMDESFDIWTHHKSDYDYALHFPTWWEQDIQAMVDKDYNHPSVILYSIG 379 LL CD++G+LV+DE FD WT K DYAL F TWWE D++AMVDKD+NHPSVILYSIG Sbjct: 333 LLDTCDQLGVLVIDELFDAWTRSKVSQDYALDFATWWESDVRAMVDKDFNHPSVILYSIG 392 Query: 380 NEIPETGSANGTAWGRKIAEKIRSLDSTRYVMNSINGMVAVMNHLQEMFQGNNNSSDTGT 439 NEIPETG+A G A R++AE IRS+DSTR+V N +NG++A L F G + D+ + Sbjct: 393 NEIPETGTAAGAAISRRLAETIRSIDSTRFVTNGVNGLLAGGPDLLASFPGESRKKDSES 452 Query: 440 -DVNSFMANLSSFTKGIMGMDVVTHSTAE 467 DVN FM +M +VV TAE Sbjct: 453 LDVNGFMTRFRELMPILMSSEVVGSKTAE 481 >A8LE96.1 A8LE96_FRASN Glycoside hydrolase family 2 sugar binding Length = 799 Score = 537 bits (1383), Expect = e-150, Method: Compositional matrix adjust. Identities = 256/452 (56%), Positives = 326/452 (72%), Gaps = 4/452 (0%) Query: 20 VTLPHDAMIAKKRSAQAVSGSKKGYFSDGAYDYVKKFYVPEEYKDKRVTFEFEGVYMHAM 79 V LPHDAM+A++R+ + ++G++ GAY Y K F+VPEEY+++RVTFEFEGVY A Sbjct: 5 VRLPHDAMVARERNRSDAAAGQRGFYPAGAYQYRKSFFVPEEYRNRRVTFEFEGVYRSAR 64 Query: 80 VYINGDFAGQHPFGYTNFYIKADRFLKYGAENEIKVVAKSHDDSRWYTGTGIYRNTKIMV 139 V++NG AGQH +GY++FY++AD FLKY +NEI V A S DD+RWY+G G+YRNTK++V Sbjct: 65 VFLNGGLAGQHAYGYSHFYVRADHFLKYNEDNEILVEAHSADDTRWYSGGGLYRNTKLIV 124 Query: 140 ADLVHIAVDGVKINATDIESKHAVVVVATDVENEGINPRTVRIVTEIVDADGNSVASDTA 199 DLVHI +DGVK+ I++ A+V VAT V NE RTV + TEIV ADG V D A Sbjct: 125 GDLVHIGLDGVKVTTPAIDADLALVAVATQVHNESSVTRTVEVTTEIVGADGVVVVRDVA 184 Query: 200 PFTAFAGEKATSRQRLYIREPKLWNVETPYLYTCISKVMDGEHVLDEETHTFGIRTLSLD 259 P T F + T RQRL + P+LW VE P+LYTC ++V +LDEE FG+R+L++D Sbjct: 185 PLTLFTDDPVTVRQRLPVPRPQLWGVEHPHLYTCRTRVTADGELLDEEATRFGVRSLTVD 244 Query: 260 AEDGLRINGEVVKLRGACIHHDNGVIGASTIERADERRIEILKQAGFNAIRSAHHPASKA 319 GLRING VV LRGACIHHDNGVIGA+TI+RA++RR+EILKQAGFNAIRS+H+P SKA Sbjct: 245 PHWGLRINGGVVNLRGACIHHDNGVIGAATIDRAEQRRVEILKQAGFNAIRSSHNPISKA 304 Query: 320 LLKACDRIGMLVMDESFDIWTHHKSDYDYALHFPTWWEQDIQAMVDKDYNHPSVILYSIG 379 LL ACDR+GMLV+DE FD WT K DYAL FP WW+ D+QAMVDKD+NHP VILYSIG Sbjct: 305 LLDACDRLGMLVIDELFDAWTRSKVAQDYALDFPLWWKSDVQAMVDKDFNHPCVILYSIG 364 Query: 380 NEIPETGSANGTAWGRKIAEKIRSLDSTRYVMNSINGMVAVMNHLQEMFQ--GNNNSSDT 437 NEIPETG+A G A R++AE+IR++D TR+V N +NG++A L F SS+ Sbjct: 365 NEIPETGTAAGAAISRQLAERIRAIDDTRFVTNGVNGLLAGGPELLASFSSGAREKSSEA 424 Query: 438 G--TDVNSFMANLSSFTKGIMGMDVVTHSTAE 467 G DVN+FM F +M ++V TAE Sbjct: 425 GEAVDVNAFMNRFREFMPILMASEMVGSKTAE 456 >A8LDF3.1 A8LDF3_FRASN Glycoside hydrolase family 2 sugar binding Length = 655 Score = 511 bits (1315), Expect = e-143, Method: Compositional matrix adjust. Identities = 247/449 (55%), Positives = 304/449 (67%), Gaps = 31/449 (6%) Query: 20 VTLPHDAMIAKKRSAQAVSGSKKGYFSDGAYDYVKKFYVPEEYKDKRVTFEFEGVYMHAM 79 V LPHDAM+ +R ++GY+ G Y Y K F+ PEEY+++RVT EFEGVY +A Sbjct: 33 VRLPHDAMVGLERDRADTEAGQRGYYPSGEYQYRKTFFAPEEYRNRRVTLEFEGVYRNAR 92 Query: 80 VYINGDFAGQHPFGYTNFYIKADRFLKYGAENEIKVVAKSHDDSRWYTGTGIYRNTKIMV 139 V+INGDFA Q +GY+NFY+ D LKYG+ NEI V A+S +D+RWY+G G+YRNTK++V Sbjct: 93 VFINGDFAAQRAYGYSNFYVHTDHLLKYGSNNEIVVEARSGNDTRWYSGGGVYRNTKLIV 152 Query: 140 ADLVHIAVDGVKINATDIESKHAVVVVATDVENEGINPRTVRIVTEIVDADGNSVASDTA 199 DLVH+A+DGV RI T VD DG VA DTA Sbjct: 153 GDLVHLALDGV------------------------------RITTPAVDDDGTVVAGDTA 182 Query: 200 PFTAFAGEKATSRQRLYIREPKLWNVETPYLYTCISKVMDGEHVLDEETHTFGIRTLSLD 259 P T F G+ T RQRL + P+LW VE PYLY C + V +LD+ET FGIRTL++D Sbjct: 183 PVTTFIGDTLTLRQRLPVPRPELWGVERPYLYLCRTSVTADGELLDQETTRFGIRTLTVD 242 Query: 260 AEDGLRINGEVVKLRGACIHHDNGVIGASTIERADERRIEILKQAGFNAIRSAHHPASKA 319 GLRINGE V LRGACIHHDNGVIGA+TI+RA++RR+EILKQAGFNAIRSAHHP SKA Sbjct: 243 PLRGLRINGETVNLRGACIHHDNGVIGAATIDRAEQRRVEILKQAGFNAIRSAHHPMSKA 302 Query: 320 LLKACDRIGMLVMDESFDIWTHHKSDYDYALHFPTWWEQDIQAMVDKDYNHPSVILYSIG 379 LL CD++G+LV+DE FD WT K DYAL F TWWE D++AMVDKD+NHPSVILYSIG Sbjct: 303 LLDTCDQLGVLVIDELFDAWTRSKVSQDYALDFATWWESDVRAMVDKDFNHPSVILYSIG 362 Query: 380 NEIPETGSANGTAWGRKIAEKIRSLDSTRYVMNSINGMVAVMNHLQEMFQGNNNSSDTGT 439 NEIPETG+A G A R++AE IRS+DSTR+V N +NG++A L F G + D+ + Sbjct: 363 NEIPETGTAAGAAISRRLAETIRSIDSTRFVTNGVNGLLAGGPDLLASFAGESRKKDSES 422 Query: 440 -DVNSFMANLSSFTKGIMGMDVVTHSTAE 467 DVN FM F +M +VV TAE Sbjct: 423 LDVNGFMTRFREFMPILMSSEVVGSKTAE 451 >A8LEG1.1 A8LEG1_FRASN Glycoside hydrolase family 2 sugar binding Length = 829 Score = 506 bits (1304), Expect = e-141, Method: Compositional matrix adjust. Identities = 241/468 (51%), Positives = 321/468 (68%), Gaps = 4/468 (0%) Query: 2 VGPVAGFFNMNSNEQPKA--VTLPHDAMIAKKRSAQAVSGSKKGYFSDGAYDYVKKFYVP 59 V P F S P A VTLPHDAMI RSA+ G++ GYF DG +YVK F VP Sbjct: 12 VRPRTSLFGEVSGNAPAAAGVTLPHDAMIVLDRSAEHSGGAETGYFPDGVVEYVKSFEVP 71 Query: 60 EEYKDKRVTFEFEGVYMHAMVYINGDFAGQHPFGYTNFYIKADRFLKYGAENEIKVVAKS 119 E Y+DKRV EFEGVY A VY+NG FAGQ P+GY+ F + D FL++G NE++V A++ Sbjct: 72 ESYRDKRVILEFEGVYRDAQVYVNGAFAGQRPYGYSLFRVSLDPFLRHGQANEVRVEARA 131 Query: 120 HDDSRWYTGTGIYRNTKIMVADLVHIAVDGVKINATDIESKHAVVVVATDVENEGINPRT 179 H DSRWY+G GI+R ++V + VHIA DGV+I D++ + AVV VATD+ N + T Sbjct: 132 HSDSRWYSGLGIHRPVSLIVNEPVHIAADGVRITTPDVDDERAVVAVATDIVNSTPHTVT 191 Query: 180 VRIVTEIVDADGNSVASDTAPFTAFAGEKATSRQRLYIREPKLWNVETPYLYTCISKVMD 239 V +V+E++ ADG VA + AP T GE AT+RQRLY+ P+ W V+ P+LY + + + Sbjct: 192 VELVSEVM-ADGERVARNAAPVTVRPGETATARQRLYVGSPRRWGVDHPHLYRLRTGLWN 250 Query: 240 GEHVLDEETHTFGIRTLSLDAEDGLRINGEVVKLRGACIHHDNGVIGASTIERADERRIE 299 L+E FGIRT+ +D GLRING VKLRGACIHHDNG++GA+ I RA+ERR+E Sbjct: 251 ANTGLEEVATDFGIRTIQVDPTHGLRINGATVKLRGACIHHDNGILGAAAISRAEERRVE 310 Query: 300 ILKQAGFNAIRSAHHPASKALLKACDRIGMLVMDESFDIWTHHKSDYDYALHFPTWWEQD 359 ILK AGFNA+R++H+P SKA+L ACDR+GMLV+DE+FD+WTH K +DY+L FP WW++D Sbjct: 311 ILKGAGFNAVRASHNPLSKAMLHACDRLGMLVLDEAFDVWTHAKMPFDYSLAFPEWWQRD 370 Query: 360 IQAMVDKDYNHPSVILYSIGNEIPETGSANGTAWGRKIAEKIRSLDSTRYVMNSINGMVA 419 I++MV KD+NHPSVILYSIGNEIP+ S G+AW R I+EKI +D TR+ N++NG +A Sbjct: 371 IESMVKKDFNHPSVILYSIGNEIPDLASPLGSAWSRDISEKIGEIDGTRFTTNAVNGFLA 430 Query: 420 VMNHLQEMFQGNNNSSDTGTDVNSFMANLSSFTKGIMGMDVVTHSTAE 467 VM+ L + TG +N+ MA++ I ++V+ T E Sbjct: 431 VMSDLVKQVDARPGPGQTG-GLNTMMASIGDVLDQINASEIVSERTRE 477 >B0RCP1.1 B0RCP1_CLAMS Putative glycosyl hydrolase Length = 875 Score = 465 bits (1196), Expect = e-129, Method: Compositional matrix adjust. Identities = 229/463 (49%), Positives = 302/463 (65%), Gaps = 14/463 (3%) Query: 18 KAVTLPHDAMIAKKRSAQAVSGSKKGYFSDGAYDYVKKFYVPEEYKDKRVTFEFEGVYMH 77 AV LPHDA+I +R A A SG+ +F DGA++Y K F VP E+ +K V+ EFEGVY Sbjct: 73 SAVRLPHDALIGLERRADAPSGAAGAFFPDGAFEYRKAFDVPSEWSEKHVSIEFEGVYRD 132 Query: 78 AMVYINGDFAGQHPFGYTNFYIKADRFLKYGAENEIKVVAKSHDDSRWYTGTGIYRNTKI 137 AMVY+NG FA Q P GY +F + D L YG EN I+V A+ HDDSRWYTG GIYR + Sbjct: 133 AMVYVNGAFAAQRPNGYASFVVDLDAHLAYGEENTIRVDARVHDDSRWYTGAGIYREVHL 192 Query: 138 MVADLVHIAVDG-VKINATDIESKHAVVVVATDVENEGINPRTVRIVTEIVDADGNSVAS 196 V+ VH+ G ++I D++ + AVV V T + N G+ TV + +E V DG V + Sbjct: 193 RVSGRVHVVEQGGLRITTPDVDDRRAVVQVETQLRNTGLRTVTVEVASEAVTPDGRVVGT 252 Query: 197 DTAPFTAFAGEKATSRQRLYIREPKLWNVETPYLYTCISKVM-DGEHVLDEETHTFGIRT 255 T+P T RQRLY+ EP+ W+V+ P L+T + V DGE LD TFGIRT Sbjct: 253 GTSPITLLPRISGVVRQRLYVAEPERWSVDRPALHTIRTSVRRDGEE-LDARETTFGIRT 311 Query: 256 LSLDAEDGLRINGEVVKLRGACIHHDNGVIGASTIERADERRIEILKQAGFNAIRSAHHP 315 L LD + GLRINGE VKLRGACIHHDNG++GA+T A+ERR+ +LK AGFNAIRS+H+P Sbjct: 312 LQLDPDHGLRINGETVKLRGACIHHDNGILGAATFADAEERRVVLLKAAGFNAIRSSHNP 371 Query: 316 ASKALLKACDRIGMLVMDESFDIWTHHKSDYDYALHFPTWWEQDIQAMVDKDYNHPSVIL 375 S A+L ACDR GMLVMDE+FD+W+ KS +DY+L FP WWE+D++AMV D NHPSVI+ Sbjct: 372 MSAAMLDACDRHGMLVMDETFDMWSESKSPFDYSLAFPEWWERDVEAMVADDINHPSVIM 431 Query: 376 YSIGNEIPETGSANGTAWGRKIAEKIRSLDSTRYVMNSINGMVAVMNHL----------- 424 YSIGNEIPETG+ G+ WGR +AE+IR LD TR++ N ING V+ + + Sbjct: 432 YSIGNEIPETGNPIGSGWGRLLAERIRELDDTRFITNGINGFVSALTEVTAMMEEAMAAA 491 Query: 425 QEMFQGNNNSSDTGTDVNSFMANLSSFTKGIMGMDVVTHSTAE 467 + ++ ++ VN+ M + +F + +VT + AE Sbjct: 492 ASVATAPSDGAEGDGGVNALMGDGDAFMDQVAVSPLVTAAIAE 534 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.134 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,969,886,073 Number of extensions: 124945567 Number of successful extensions: 297999 Number of sequences better than 1.0e-05: 1469 Number of HSP's gapped: 294637 Number of HSP's successfully gapped: 1500 Length of query: 496 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 356 Effective length of database: 1,741,754,103 Effective search space: 620064460668 Effective search space used: 620064460668 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)