BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0251.0029.p (304 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A8LE96.1 A8LE96_FRASN Glycoside hydrolase family 2 sugar binding 324 6e-87 A8LEC9.1 A8LEC9_FRASN Glycoside hydrolase family 2 sugar binding 318 4e-85 B7Y054.1 B7Y054_RUMHA Putative uncharacterized protein 252 3e-65 B0RCP1.1 B0RCP1_CLAMS Putative glycosyl hydrolase 241 7e-62 A8LEG1.1 A8LEG1_FRASN Glycoside hydrolase family 2 sugar binding 233 2e-59 >A8LE96.1 A8LE96_FRASN Glycoside hydrolase family 2 sugar binding Length = 799 Score = 324 bits (830), Expect = 6e-87, Method: Compositional matrix adjust. Identities = 162/308 (52%), Positives = 207/308 (67%), Gaps = 5/308 (1%) Query: 1 VICGSETFPRDIANNWKLVKENGHVIGDFTWTGWDYLGEAGLGKTVYDETAAQGATA--- 57 VI G+ET+P +I NW+LV++N HVIGDFTWTGWDYLGE G+G+ Y +T+ Sbjct: 484 VIVGTETYPSEIDRNWRLVQDNSHVIGDFTWTGWDYLGEPGIGRIEYQGDEENASTSPSH 543 Query: 58 -SYPWKTAYCGDIDITGYRRPVSYYREIVYGLRKQPFIAVQRPEHYGKKPGMTPXXXXXX 116 SYPW TA+CGDIDITG+RRP SYYREIV+GLR +P+IAV RP+ YG+ + Sbjct: 544 GSYPWLTAWCGDIDITGHRRPASYYREIVFGLRSEPYIAVHRPDRYGQPVTVAMWWSWSD 603 Query: 117 XXXXXXXR-YEGKPVKVEVYSEADEVELLINGKTAGRAAAGEAHEFKAEFDIVYAPGELV 175 +E +PV+VEVYS ADEVELL+NG+ G AGE + FKAEFD VY PGE+V Sbjct: 604 AISSWSWDGHETRPVRVEVYSAADEVELLVNGRLIGTVPAGEKNRFKAEFDTVYEPGEIV 663 Query: 176 AIAYTNGKESGRTSLLTAVGGVNLQVAIDRPQIVSDDNDLSFVTISLVGDNGVVRPLDDR 235 A+AYT G+E+GRT L +A G V L VA DR IV+DD DL+++ I+LV + G + DR Sbjct: 664 AVAYTAGRETGRTLLRSATGEVRLAVAADRTDIVADDTDLAYIAITLVDEAGNLYNTADR 723 Query: 236 KVSVHVEGAGILQGFGSANPKSXXXXXXXXXXXXXGRALAIIRPTNSGIINVLIEAEGCT 295 V+V V G G+LQGFGSA+PK+ GRALA+IRPT G I V + A+GC Sbjct: 724 TVAVEVAGPGVLQGFGSADPKTEENFFDTTRATFDGRALAVIRPTAPGTITVTLTAQGCE 783 Query: 296 AQNVKIEA 303 ++IEA Sbjct: 784 PSTIRIEA 791 >A8LEC9.1 A8LEC9_FRASN Glycoside hydrolase family 2 sugar binding Length = 825 Score = 318 bits (815), Expect = 4e-85, Method: Compositional matrix adjust. Identities = 162/309 (52%), Positives = 205/309 (66%), Gaps = 6/309 (1%) Query: 1 VICGSETFPRDIANNWKLVKENGHVIGDFTWTGWDYLGEAGLGKTVY---DET--AAQGA 55 VI G+ET+P DI NW+LV+++ HVIGDFTWTGWDYLGE G G+ Y DET +A Sbjct: 509 VIVGTETYPADIDTNWRLVQDHSHVIGDFTWTGWDYLGEPGTGRIEYEGDDETTNSALNH 568 Query: 56 TASYPWKTAYCGDIDITGYRRPVSYYREIVYGLRKQPFIAVQRPEHYGKKPGMTPXXXXX 115 YPW TA+CGDIDITG+RRP SYYREIV+GLR +P+IAV RPE +G+ + Sbjct: 569 QGGYPWLTAWCGDIDITGHRRPASYYREIVFGLRGKPYIAVHRPERFGRAVRVAMWWSWS 628 Query: 116 XXXXXXXXR-YEGKPVKVEVYSEADEVELLINGKTAGRAAAGEAHEFKAEFDIVYAPGEL 174 +E +PV++EVYS ADE+ELL+NG+ G A AGE + FKAEF+ VY PGE+ Sbjct: 629 DSVSSWSWNGHEDRPVRLEVYSAADEIELLVNGRRVGTAPAGEKNRFKAEFETVYEPGEI 688 Query: 175 VAIAYTNGKESGRTSLLTAVGGVNLQVAIDRPQIVSDDNDLSFVTISLVGDNGVVRPLDD 234 VA+AYT G+E+GRTSL +A G V L A DR +I +DD DL++V ++LV G + D Sbjct: 689 VAVAYTGGRETGRTSLRSATGEVRLDAAADRTRITADDTDLAYVALTLVDGAGNLYNTAD 748 Query: 235 RKVSVHVEGAGILQGFGSANPKSXXXXXXXXXXXXXGRALAIIRPTNSGIINVLIEAEGC 294 RKV+V V G +LQGFGSA+P+ GRALA+IRPT G I V AEGC Sbjct: 749 RKVAVEVTGPAVLQGFGSADPRPTENFFDTVRTTFDGRALAVIRPTAPGSITVTATAEGC 808 Query: 295 TAQNVKIEA 303 V+IEA Sbjct: 809 EPSTVRIEA 817 >B7Y054.1 B7Y054_RUMHA Putative uncharacterized protein Length = 827 Score = 252 bits (644), Expect = 3e-65, Method: Compositional matrix adjust. Identities = 134/302 (44%), Positives = 182/302 (60%), Gaps = 3/302 (0%) Query: 2 ICGSETFPRDIANNWKLVKENGHVIGDFTWTGWDYLGEAGLGKTVYDETAAQGATASYPW 61 + G+ETFP DI W LV++ H+IGDFTWTG+DYLGEAG G YD T G+ YP Sbjct: 526 VVGTETFPADIFRLWTLVEDYSHIIGDFTWTGYDYLGEAGCGIFYYDGTKNFGSV--YPD 583 Query: 62 KTAYCGDIDITGYRRPVSYYREIVYGLRKQPFIAVQRPEHYGKKPGMTPXXXXXXXXXXX 121 + AY GDI ITG RRP+SYYRE+VYGLR +P+IAV+R + G+ P TP Sbjct: 584 RLAYIGDITITGVRRPISYYREVVYGLRHEPYIAVERVDRNGQTPSKTPWMFKDNISSWT 643 Query: 122 XXRYEGKPVKVEVYSEADEVELLINGKTAGRAAAGEAHEFKAEFDIVYAPGELVAIAYTN 181 YEG V+VYS+A+EVEL +N ++ GR AG+ +++ AEF + Y G L+AI Y N Sbjct: 644 WTGYEGTSASVDVYSDAEEVELFLNDESLGRKKAGKENQYMAEFTVPYQAGTLMAIDYVN 703 Query: 182 GKESGRTSLLTAVGGVNLQVAIDRPQIVSDDNDLSFVTISLVGDNGVVRPLDDRKVSVHV 241 G E GR SL TA L V +D+ + ++ DL ++ I+L ++G + ++++V V Sbjct: 704 GMERGRYSLETAKKSTTLDVKMDKKVLTANGQDLCYLNITLCDESGKTNQQEIKQITVSV 763 Query: 242 EGAGILQGFGSANPKSXXXXXXXXXXXXXGRALAIIRPTN-SGIINVLIEAEGCTAQNVK 300 +GAG LQG GS+NP + G A+A +R + SGII V I A GC K Sbjct: 764 DGAGKLQGLGSSNPSNEEDYFDRCCNTFDGDAMACVRAGHESGIITVTISANGCEDVVRK 823 Query: 301 IE 302 IE Sbjct: 824 IE 825 >B0RCP1.1 B0RCP1_CLAMS Putative glycosyl hydrolase Length = 875 Score = 241 bits (614), Expect = 7e-62, Method: Compositional matrix adjust. Identities = 125/303 (41%), Positives = 174/303 (57%), Gaps = 1/303 (0%) Query: 1 VICGSETFPRDIANNWKLVKENGHVIGDFTWTGWDYLGEAGLGKTVYDETAAQGATASYP 60 ++ G+ETFP I NW+LV +N HVIGDFTW+GWDYLGE G+G+ Y E + G YP Sbjct: 562 IVVGTETFPSRIDANWRLVTDNPHVIGDFTWSGWDYLGEVGVGRVKY-EGESTGFDGEYP 620 Query: 61 WKTAYCGDIDITGYRRPVSYYREIVYGLRKQPFIAVQRPEHYGKKPGMTPXXXXXXXXXX 120 W T++ D+D+TG+RRP+SYYRE V+GLR PFIAV+RPE++G+ + Sbjct: 621 WLTSWSADLDMTGHRRPMSYYRETVFGLRSTPFIAVRRPENHGRAFTLGQWSWTDAIESW 680 Query: 121 XXXRYEGKPVKVEVYSEADEVELLINGKTAGRAAAGEAHEFKAEFDIVYAPGELVAIAYT 180 EG P+ +EVYS+ADE+ELL++G+ R G F A+F++ Y G L A+A+T Sbjct: 681 SWDAEEGTPMILEVYSDADEIELLLDGEVHARVQVGGRRRFVADFELPYRRGTLTAVAFT 740 Query: 181 NGKESGRTSLLTAVGGVNLQVAIDRPQIVSDDNDLSFVTISLVGDNGVVRPLDDRKVSVH 240 G E+GR L +A V L V +R + + D LSFV I L G + DR V+V Sbjct: 741 GGVETGRADLRSASDDVVLAVRAERETVGTGDGALSFVEIELRDREGALANHRDRPVTVQ 800 Query: 241 VEGAGILQGFGSANPKSXXXXXXXXXXXXXGRALAIIRPTNSGIINVLIEAEGCTAQNVK 300 V+G G L GS P + GR LA++RP G ++V + A V+ Sbjct: 801 VDGPGSLVALGSGRPLTTERFDGDTHGTYDGRVLAVVRPDGEGDLHVTVTAPELAPVTVR 860 Query: 301 IEA 303 + A Sbjct: 861 VRA 863 >A8LEG1.1 A8LEG1_FRASN Glycoside hydrolase family 2 sugar binding Length = 829 Score = 233 bits (593), Expect = 2e-59, Method: Compositional matrix adjust. Identities = 125/276 (45%), Positives = 161/276 (58%), Gaps = 2/276 (0%) Query: 1 VICGSETFPRDIANNWKLVKENGHVIGDFTWTGWDYLGEAGLGKTVY--DETAAQGATAS 58 VI G+E+ I W LV+EN VIGDFTWTGWDYLGEAG+G+ Y +++A G A Sbjct: 505 VILGTESHGPRIDRIWHLVEENSRVIGDFTWTGWDYLGEAGIGRVSYPGEQSAEGGLLAP 564 Query: 59 YPWKTAYCGDIDITGYRRPVSYYREIVYGLRKQPFIAVQRPEHYGKKPGMTPXXXXXXXX 118 +P A GDIDITG+RRP+SY+RE V+GLR QP++AV RP+++G+K P Sbjct: 565 FPSILAGSGDIDITGFRRPMSYFRETVFGLRSQPYVAVHRPQYHGRKRVAGPWSWSDTVS 624 Query: 119 XXXXXRYEGKPVKVEVYSEADEVELLINGKTAGRAAAGEAHEFKAEFDIVYAPGELVAIA 178 G PV VEVYS A+EVELL+N ++ GR + G F FD Y PG L A+A Sbjct: 625 SWAWGIASGSPVVVEVYSNAEEVELLLNTRSLGRKSVGSERAFSVFFDCAYEPGRLTAVA 684 Query: 179 YTNGKESGRTSLLTAVGGVNLQVAIDRPQIVSDDNDLSFVTISLVGDNGVVRPLDDRKVS 238 YT G E GRTSL ++ GG L V DR + +DD DL+F+ I LV G + ++ Sbjct: 685 YTAGVEQGRTSLQSSEGGTRLAVRADRTTLRADDTDLAFLEIELVDTAGTCLVDRELEIV 744 Query: 239 VHVEGAGILQGFGSANPKSXXXXXXXXXXXXXGRAL 274 V VEG +LQ GSA P + GR L Sbjct: 745 VVVEGPAVLQALGSARPDTEERYDGHTHRTYDGRLL 780 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.136 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,718,292,585 Number of extensions: 67910762 Number of successful extensions: 140087 Number of sequences better than 1.0e-05: 209 Number of HSP's gapped: 139784 Number of HSP's successfully gapped: 209 Length of query: 304 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 169 Effective length of database: 1,788,859,178 Effective search space: 302317201082 Effective search space used: 302317201082 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)