BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0251.0024.p (289 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DGT7.1 B1DGT7_9BACL Binding-protein-dependent transport system... 484 e-135 B3KKB6.1 B3KKB6_9BACI Binding-protein-dependent transport system... 222 3e-56 A9KKJ0.1 A9KKJ0_CLOPH Binding-protein-dependent transport system... 204 5e-51 B8I4H8.1 B8I4H8_CLOCE Binding-protein-dependent transport system... 199 3e-49 C4BFJ3.1 C4BFJ3_9MICO Carbohydrate ABC transporter membrane prot... 197 1e-48 >B1DGT7.1 B1DGT7_9BACL Binding-protein-dependent transport systems inner membrane component Length = 291 Score = 484 bits (1246), Expect = e-135, Method: Compositional matrix adjust. Identities = 241/291 (82%), Positives = 255/291 (87%), Gaps = 2/291 (0%) Query: 1 MLASRGEKVFVGIITGLLILFSIIALIPLXXXXXXXXXXXXXXDMNLVTLWPKQFTLDSW 60 M SR EKVFV IT +LIL S IA+IPL DMNLVTLWPKQFT DSW Sbjct: 1 MFESRSEKVFVSGITIILILLSAIAIIPLLSVISTSLSSKSAVDMNLVTLWPKQFTFDSW 60 Query: 61 KYIVDRPDLWKSFFLTLSTTLIGTVLALLMTALFAYPLSK--PEFRWGSVIMLAVVIAMI 118 YI+DRPDLWKSFFLTL TT+IGT +ALLMTAL AYPLSK EFRWG V+M+ VVIAMI Sbjct: 61 AYIIDRPDLWKSFFLTLVTTVIGTAIALLMTALLAYPLSKTKSEFRWGGVVMIGVVIAMI 120 Query: 119 FKAPIVPYFLTVRGIGLYDNPLVLIVPHILNPFNLIIMRTFFKQFSKELEEAAFLEGCGY 178 FKAPIVPYFLTVRGIGLY+NPLVLIVPHILNPFNLIIMRTFFKQFSKELEEAAFLEGCGY Sbjct: 121 FKAPIVPYFLTVRGIGLYNNPLVLIVPHILNPFNLIIMRTFFKQFSKELEEAAFLEGCGY 180 Query: 179 FRMLFQFVLPLSKAVLATLALFYGVVLWNQFQHPLFFLQDTNWFPLQIKIRQFITDDSVI 238 FRMLFQ VLPLSKAVLATLALFYGV+LWNQFQHPLFFLQ+ +WFPLQIKIRQFITDDSVI Sbjct: 181 FRMLFQLVLPLSKAVLATLALFYGVLLWNQFQHPLFFLQNPDWFPLQIKIRQFITDDSVI 240 Query: 239 MAGAASTAHLNYNERTLRAATVIFAIVPIVVVYPFLQKYFVKGAMIGSVKG 289 MAG+ASTA LNYN RTLRAATVIFAIVPI++VYPFLQKYFVKGAMIGSVKG Sbjct: 241 MAGSASTADLNYNSRTLRAATVIFAIVPIILVYPFLQKYFVKGAMIGSVKG 291 >B3KKB6.1 B3KKB6_9BACI Binding-protein-dependent transport systems inner membrane component Length = 281 Score = 222 bits (566), Expect = 3e-56, Method: Compositional matrix adjust. Identities = 109/267 (40%), Positives = 171/267 (64%) Query: 22 SIIALIPLXXXXXXXXXXXXXXDMNLVTLWPKQFTLDSWKYIVDRPDLWKSFFLTLSTTL 81 ++ L PL D V L P +FT SW++++ LW SF +T+ T+ Sbjct: 14 AVTMLFPLLQLLAVSLSSPVAADSKEVFLLPVEFTTASWEHVLKNKGLWTSFGITVYITI 73 Query: 82 IGTVLALLMTALFAYPLSKPEFRWGSVIMLAVVIAMIFKAPIVPYFLTVRGIGLYDNPLV 141 GT+L++L + + AYPLS+ EF +M +V+ MIF AP++P+FLTVR +G+ ++ Sbjct: 74 AGTLLSMLFSVMTAYPLSRREFLIRKQVMFGIVLTMIFNAPMIPFFLTVRELGMMNSLWA 133 Query: 142 LIVPHILNPFNLIIMRTFFKQFSKELEEAAFLEGCGYFRMLFQFVLPLSKAVLATLALFY 201 LI+P ++ FN+II+RTFF EL+++A ++GC F +LF+ LPLSK V+AT++LFY Sbjct: 134 LIIPGLVGTFNMIIIRTFFMGIPSELDDSARIDGCSEFGILFRMYLPLSKPVMATVSLFY 193 Query: 202 GVVLWNQFQHPLFFLQDTNWFPLQIKIRQFITDDSVIMAGAASTAHLNYNERTLRAATVI 261 V WN F + F++D +PLQ+K+R ++ + A ++N TL+AAT++ Sbjct: 194 AVGYWNTFSRAVLFIRDPEKWPLQMKLRAYLQSPEELAAVNLFLGDYDFNTTTLKAATIL 253 Query: 262 FAIVPIVVVYPFLQKYFVKGAMIGSVK 288 FA +PI++VYP+LQKYFVKG+M+GS+K Sbjct: 254 FATIPIILVYPYLQKYFVKGSMLGSLK 280 >A9KKJ0.1 A9KKJ0_CLOPH Binding-protein-dependent transport systems inner membrane component Length = 281 Score = 204 bits (520), Expect = 5e-51, Method: Compositional matrix adjust. Identities = 106/282 (37%), Positives = 173/282 (61%), Gaps = 3/282 (1%) Query: 7 EKVFVGIITGLLILFSIIALIPLXXXXXXXXXXXXXXDMNLVTLWPKQFTLDSWKYIVDR 66 ++++ I T LL L +I ++PL + N V WPK F ++++ + Sbjct: 2 RRMYIWISTILLSLLCMIMVLPLIKVVSESFSSKTFIEQNAVLFWPKGFNVEAYSKVFQN 61 Query: 67 PDLWKSFFLTLSTTLIGTVLALLMTALFAYPLSKPEFRWGSVIMLAVVIAMIFKAPIVPY 126 +++ + ++ TL+GT + L+MTA AYPLS+ EF++ I+L V + MIF +P++P Sbjct: 62 RNIFVALRNSIVVTLLGTTINLVMTATMAYPLSRTEFKFKKPILLMVTLTMIFTSPMIPS 121 Query: 127 FLTVRGIGLYDNPLVLIVPHILNPFNLIIMRTFFKQFSKELEEAAFLEGCGYFRMLFQFV 186 +L ++G+GL ++ +I+P +++ +N IMR+FF EL +AA ++G G F +LF+ V Sbjct: 122 YLVIKGMGLDNSIWAVILPGMISGYNFFIMRSFFAGVPGELIDAARIDGSGEFGILFKIV 181 Query: 187 LPLSKAVLATLALFYGVVLWNQFQHPLFFLQDTNWFPLQIKIRQFITDDSVIMAGAASTA 246 LP+SKAV+AT+ LFYGV WN Q PL +L+ LQIK+ Q + +D+ M Sbjct: 182 LPMSKAVMATMILFYGVNHWNALQQPLIYLRSPKVQTLQIKLYQILMEDNTSMVDVGV-- 239 Query: 247 HLNYNERTLRAATVIFAIVPIVVVYPFLQKYFVKGAMIGSVK 288 L T++ AT++ + +PI++VYPFLQK+F GAM+GSVK Sbjct: 240 -LQIAPTTIKMATIVISTIPILIVYPFLQKHFAAGAMLGSVK 280 >B8I4H8.1 B8I4H8_CLOCE Binding-protein-dependent transport systems inner membrane component Length = 290 Score = 199 bits (505), Expect = 3e-49, Method: Compositional matrix adjust. Identities = 108/290 (37%), Positives = 164/290 (56%), Gaps = 1/290 (0%) Query: 1 MLASRGEKVFVGIITGLLILFSIIALIPLXXXXXXXXXXXXXXDMNLVTLWPKQFTLDSW 60 M+ +KVF I + ++ I+ L P+ + V +WP F ++S+ Sbjct: 1 MIYKTSDKVFNIFINSITLISVILCLFPVLNVVAVSLSSNSAILASKVFVWPIDFNIESY 60 Query: 61 KYIVDRPDLWKSFFLTLSTTLIGTVLALLMTALFAYPLSKPEFRWGSVIMLAVVIAMIFK 120 K I+ + S T+ T T + + +T AYPL+K R + IML ++ M F Sbjct: 61 KAILKDTTMIWSLVYTIIITFAYTAIGMFLTICAAYPLTKQRLRGRNAIMLMIIFTMYFS 120 Query: 121 APIVPYFLTVRGIGLYDNPLVLIVPHILNPFNLIIMRTFFKQFSKELEEAAFLEGCGYFR 180 ++P ++ V+ + + D+ LI+P +L+ FNLI+++TFF + LEE+A L+GC Sbjct: 121 GGLIPDYMLVKNLNMLDSSWSLILPGVLSAFNLIVLKTFFTSLPESLEESAILDGCNDIG 180 Query: 181 MLFQFVLPLSKAVLATLALFYGVVLWNQFQHPLFFLQDTNWFPLQIKIRQFITDDSVIMA 240 +LF+ VLPLSK VLATL+LFY V WN F LF++ D+ +PLQ+K+ Q I + + A Sbjct: 181 VLFKIVLPLSKPVLATLSLFYAVGKWNSFMDALFYISDSKLYPLQLKLYQIIVNSQTVDA 240 Query: 241 GAASTA-HLNYNERTLRAATVIFAIVPIVVVYPFLQKYFVKGAMIGSVKG 289 N +L+AA V+FA +PIV+VYP LQKYFV G MIG+VKG Sbjct: 241 SIGDGGVSQNLPPESLKAACVVFATLPIVLVYPKLQKYFVSGIMIGAVKG 290 >C4BFJ3.1 C4BFJ3_9MICO Carbohydrate ABC transporter membrane protein 2, CUT1 family Length = 320 Score = 197 bits (500), Expect = 1e-48, Method: Compositional matrix adjust. Identities = 105/286 (36%), Positives = 173/286 (60%), Gaps = 1/286 (0%) Query: 4 SRGEKVFVGIITGLLILFSIIALIPLXXXXXXXXXXXXXXDMNLVTLWPKQFTLDSWKYI 63 +RGEK F I +L F+++ +IP + L P++FTLD+++YI Sbjct: 36 TRGEKGFRVINVIILSGFALLCVIPFVHVIGSSFATPGELATSNFLLVPREFTLDAYRYI 95 Query: 64 VDRPDLWKSFFLTLSTTLIGTVLALLMTALFAYPLSKPEFRWGSVIMLAVVIAMIFKAPI 123 + P ++++ ++ T+ GT L+LL+TAL AY L+KP VI VV M+F + Sbjct: 96 LSTPTIFRAIGVSTIVTVGGTFLSLLVTALMAYALAKPRLVGRRVINFMVVFTMLFSGGM 155 Query: 124 VPYFLTVRGIGLYDNPLVLIVPHILNPFNLIIMRTFFKQFSKELEEAAFLEGCGYFRMLF 183 +P F+ V +GL D+ L++P ++N FN +IMR FF + LEE+A ++GC + Sbjct: 156 IPTFIVVSKLGLLDSLWSLVLPVLINAFNFVIMRNFFSGIPESLEESARIDGCSELGVFV 215 Query: 184 QFVLPLSKAVLATLALFYGVVLWNQFQHPLFFLQDTNWFPLQIKIRQFITDDSVIMAGAA 243 + VLPLS A +AT+ LFYGV WN + H + ++ D++ +P+Q+ +RQ + S + A A Sbjct: 216 RIVLPLSIASIATIGLFYGVTYWNTYMHAVLYINDSSKWPIQVLLRQIVIVASGVNADAG 275 Query: 244 STAHLNYNERTLRAATVIFAIVPIVVVYPFLQKYFVKGAMIGSVKG 289 + + ++++ A + A +P+++VYPF+Q+YFVKGA+IGSVKG Sbjct: 276 AV-DVVPPAQSVKMAVITVATLPMLMVYPFIQRYFVKGALIGSVKG 320 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.332 0.145 0.449 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,524,966,153 Number of extensions: 56882830 Number of successful extensions: 235380 Number of sequences better than 1.0e-05: 5569 Number of HSP's gapped: 228885 Number of HSP's successfully gapped: 5570 Length of query: 289 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 155 Effective length of database: 1,798,280,193 Effective search space: 278733429915 Effective search space used: 278733429915 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 130 (54.7 bits)